Article(id=1236367339789284105, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.04.020, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1656950400000, receivedDateStr=2022-07-05, revisedDate=1662480000000, revisedDateStr=2022-09-07, acceptedDate=null, acceptedDateStr=null, onlineDate=1772702544399, onlineDateStr=2026-03-05, pubDate=1682352000000, pubDateStr=2023-04-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772702544399, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772702544399, creator=13701087609, updateTime=1772702544399, updator=13701087609, issue=Issue{id=1236367331312595268, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='4', pageStart='661', pageEnd='866', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772702542379, creator=13701087609, updateTime=1772702636855, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236367727644955170, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236367727644955171, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=825, endPage=833, ext={EN=ArticleExt(id=1236367340015776530, articleId=1236367339789284105, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Ultrasound-assisted Extraction Process Optimization of Chlorogenic Acid from Solanum xanthocarpum Fruits and Its Antioxidant Activity, columnId=1236286112713470633, journalTitle=Chinese Journal of Tropical Crops, columnName=Post-harvest Treatment & Quality Safety, runingTitle=null, highlight=null, articleAbstract=

Solanum xanthocarpum fruits are used as the test material, and the extraction process of chlorogenic acid is studied in detail by using the ultrasonic-assisted ethanol extraction test method. Values of four group parameters for the volume solubility of ethanol aqueous solution, ratio of liquid to the material, ultrasonic time, ultrasonic temperature were adjusted, and then the proposed extraction process parameters of chlorogenic acid from single-factor experiments and the corresponding results were optimized. The optimized parameters were ethanol concentration 65%, ratio of liquid to material 50 mL/g, ultrasonic temperature 60 ℃ and ultrasonic time 40 min. On the basis of the above single factor optimization results, the final quadratic multiphase mathematical model was obtained further by optimization from the Box-Behnken response surface method. The extraction process parameters were ethanol concentration 64.7%, liquid-to-solid ratio 55.8 mL/g, and ultrasonic temperature 56.0 ℃ for 40.5 min, and the optimal extraction rate of chlorogenic acid was 6.81%. The extract of S. xanthocarpum fruits had good antioxidant capacity to DPPH and ABTS free radical scavenging with IC₅₀ values of 55.7 mg/L and 20.1 mg/L, respectively.

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以黄果茄（Solanum xanthocarpum）果实为试材，采用超声波辅助乙醇提取法，对绿原酸的提取工艺进行研究。通过调整乙醇水溶液体积浓度、液体与物料的比值、超声时间、超声温度等4组单因素实验的数值及结果，优化出拟定的绿原酸提取工艺参数，即乙醇溶液浓度、液料比值、超声时间、超声温度分别为65%、50 mL/g、40 min、60 ℃。在上述单因素优化结果基础上，进一步通过Box-Behnken响应面法优化得到最终的二次多项数学模型。研究结果表明，提取工艺参数为64.7%的乙醇浓度、55.8 mL/g的液料比值、56.0 ℃下超声40.5 min，黄果茄绿原酸提取率最佳，为6.81%。黄果茄果中的提取物具有较好的抗氧化能力，相应的1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-pierylhydrazy, DPPH）和2,2-连氮基-双-（3-乙基苯并二氢噻唑啉-6-磺酸）[2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate), ABTS]自由基清除能力的IC₅₀分别为55.7 mg/L和20.1 mg/L。

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郭蒙（1979—），男，博士，副教授，研究方向：药物分析与质量控制。

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郭蒙（1979—），男，博士，副教授，研究方向：药物分析与质量控制。

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