热带作物学报

桃蚜取食抗、感辣椒品种的茉莉酸途径基因表达差异分析

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税军 ¹^,²^,³ , 刘小强 ²^,³ , 陈青 ²^,³^,^* , 梁晓 ²^,³ , 伍春玲 ²^,³ , 刘迎 ²^,³ , 姚晓文 ¹^,²^,³ , 乔阳 ¹^,²^,³ , 毛立杰 ¹^,²^,³ , 陈银华 ¹

热带作物学报 | 植物保护与生物安全 2023,44(4): 774-784

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热带作物学报 | 植物保护与生物安全 2023, 44(4): 774-784

桃蚜取食抗、感辣椒品种的茉莉酸途径基因表达差异分析

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税军¹^,²^,³, 刘小强²^,³, 陈青²^,³^,^*, 梁晓²^,³, 伍春玲²^,³, 刘迎²^,³, 姚晓文¹^,²^,³, 乔阳¹^,²^,³, 毛立杰¹^,²^,³, 陈银华¹

作者信息

^1.海南大学热带作物学院，海南海口　570228

^2.中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室/海南省热带作物病虫害生物防治工程技术研究中心，海南海口　571101

^3.中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室，海南三亚　572000

税军（1997—），男，硕士，研究方向：抗虫种质资源挖掘与创新利用。

通讯作者:

^* 陈青（CHENQing），E-mail: chqingztq@163.com。

Differential Expression Analysis of Jasmonic Acid Pathway Genes Between Resistant and Susceptible Pepper Cultivars Against Myzus persicae Infestation

Jun SHUI¹^,²^,³, Xiaoqiang LIU²^,³, Qing CHEN²^,³^,^*, Xiao LIANG²^,³, Chunling WU²^,³, Ying LIU²^,³, Xiaowen YAO¹^,²^,³, Yang QIAO¹^,²^,³, Lijie MAO¹^,²^,³, Yinhua CHEN¹

Affiliations

^1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China

^2.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, Hainan 571101, China

^3.Sanya Research Academy, Chinese Academy of Tropical Agriculture Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan 572000, China

出版时间: 2023-04-25 doi: 10.3969/j.issn.1000-2561.2023.04.014

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摘要

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根据蚜口密度及取食时间效应，明确桃蚜取食前后辣椒茉莉酸（JA）合成及信号途径基因表达差异，初步阐明JA途径介导的辣椒抗蚜性分子机理。本研究以抗蚜辣椒品种猪大肠（ZDC）和感蚜辣椒品种大羊角椒（DYJJ）为材料，采用实时定量PCR技术分析与植物防御相关的JA合成途径基因（LOX2、AOS、AOC和OPR3）与信号途径基因（COI1和JAR1）在桃蚜不同蚜口密度（20、30、40、50、60蚜/叶）及取食不同时间（6、12、24、48、72 h）后的表达量变化情况。结果表明：在不同蚜口密度和取食为害时间下，JA合成途径的4个基因在ZDC中表达量总体上显著高于DYJJ，而信号途径2个基因表达量总体上显著低于DYJJ。在桃蚜较低为害水平状态下，抗、感辣椒品种之间表达量差异显著，且在部分相同蚜口密度下部分基因表达量峰值和低谷出现时间基本一致。较高水平的为害情况下，抗、感辣椒品种之间差异显著性下降。以上结果表明，JA合成及信号途径AOS、AOC及JAR1基因可能具有作为鉴定评价辣椒抗蚜性水平的分子指标的潜力，可为将来辣椒抗蚜种质资源评价与创新利用提供理论依据。

关键词

桃蚜 / 抗、感辣椒品种 / 密度 / 取食时间 / 茉莉酸 / 合成途径 / 信号途径 / 基因表达

Abstract

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In order to clarify the difference of jasmonic acid (JA) synthesis and signal pathway genes expression in pepper before and after aphid feeding, the molecular mechanism of JA pathway-mediated resistance to aphid in pepper was preliminarily clarified based on the effects of aphid densities and infestation time. The aphid-resistant pepper variety ZDC and aphid-susceptible pepper variety DYJJ were used as the materials. The expression changes of JA synthesis pathway genes (LOX2, AOS, AOC and OPR3) and signal pathway genes (COI1 and JAR1) related to plant defense were analyzed by real-time quantitative PCR after aphid density (20, 30, 40, 50 and 60 aphids/leaf) and feeding time (6, 12, 24, 48 and 72 h). The results showed that under different aphid population densities and infestation time, the expression levels of the four genes in JA synthesis pathway in ZDC were generally significantly higher than those in DYJJ, while the expression levels of the two genes in signal pathway were generally significantly lower than those in DYJJ. Under the condition of low aphid damage level, there were significant differences in the expression levels between resistant and susceptible pepper varieties, and the peak and trough time of some gene expression were basically the same under the same aphid population density. Under high level of damage, the difference between resistant and susceptible pepper varieties decreased significantly. The above results showed that JA synthesis and signaling pathways AOS, AOC and JAR1 genes might have the potential to be used as molecular indicators for the identification and evaluation of aphid resistance in pepper, which could provide a theoretical basis for the evaluation and innovative utilization of aphid-resistant germplasm resources in pepper in the future.

Key words

Myzus persicae / resistant and susceptible pepper varieties / density / feeding time / jasmonic acid / synthesis pathway / signal pathway / gene expression

引用本文

税军, 刘小强, 陈青, 梁晓, 伍春玲, 刘迎, 姚晓文, 乔阳, 毛立杰, 陈银华. 桃蚜取食抗、感辣椒品种的茉莉酸途径基因表达差异分析. 热带作物学报, 2023 , 44 (4) : 774 -784 . DOI: 10.3969/j.issn.1000-2561.2023.04.014

Jun SHUI, Xiaoqiang LIU, Qing CHEN, Xiao LIANG, Chunling WU, Ying LIU, Xiaowen YAO, Yang QIAO, Lijie MAO, Yinhua CHEN. Differential Expression Analysis of Jasmonic Acid Pathway Genes Between Resistant and Susceptible Pepper Cultivars Against Myzus persicae Infestation[J]. Chinese Journal of Tropical Crops, 2023 , 44 (4) : 774 -784 . DOI: 10.3969/j.issn.1000-2561.2023.04.014

基金

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海南省重点研发计划项目(ZDYF2020064)
农业农村部农业资源调查与保护利用专项(NFZX-2021)

参考文献

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2023年第44卷第4期

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文章信息

doi: 10.3969/j.issn.1000-2561.2023.04.014

接收时间：2022-06-21
首发时间：2026-03-05
出版时间：2023-04-25

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出版历史

收稿日期：2022-06-21
修回日期：2022-09-19

基金

海南省重点研发计划项目(ZDYF2020064)

农业农村部农业资源调查与保护利用专项(NFZX-2021)

作者信息

^1.海南大学热带作物学院，海南海口　570228

^3.中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室，海南三亚　572000

通讯作者:

^* 陈青（CHENQing），E-mail: chqingztq@163.com。

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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.04.014

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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