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It is normally difficult to distinguish germplasms of Kadsura coccinea with morphological traits before they bear fruits because the evaluations on the specificity are mainly based on fruit and economic traits. Establishing DNA fingerprinting profile is useful for rapid and accurate identification of K. Coccinea germplasms. SSR analysis for 20 clones of K. coccinea was conducted using 15 pairs of primers, the clones were clustered based on Nei's (1972) genetic distance by UPGMA clustering method, and SSR fingerprinting profile was established through combinations of some markers. 15 pairs of primers produced 75 alleles at 15 loci from 20 clones of K. coccinea, with two to 15 alleles per locus, the Shannon diversity index varied from 0.199 to 2.456, polymorphic information index from 0.095 to 0.894, and genetic similarity coefficient from 0.328 to 0.891. The 20 clones could be divided into two groups by the UPGMA clustering method. All the 15 loci could not distinguish 20 clones separately, among which KCZ023 showed the strongest ability to distinguish. Two-loci combinations of KCZ023 with KCZ135, KCZ100 or KCZ147 as well as 28 three-loci combinations could distinguish all the 20 clones separately. The 20 clones of K. coccinea show abundant genetic diversity, and can be fully distinguished by any of three two-loci combinations or 28 three-loci combinations. The findings would provide evidences for germplasm identification and hybrid parent selection.

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由于黑老虎种质的特异性评价以其果实及经济性状为主，未结实时仅依据形态性状较难区分，因此，构建黑老虎DNA指纹图谱有助于黑老虎种质的快速精准鉴定。选择15对引物对20个黑老虎品系进行SSR分析，依据Nei's（1972）遗传距离，采用非加权组平均法（UPGMA）进行聚类分析，通过位点间的组合构建SSR指纹图谱。结果表明：15对引物在20个黑老虎品系中共扩增出15个位点75个等位基因，每个位点的等位基因数为2~15，其Shannon信息指数（I）为0.199~2.456，多态信息含量（PIC）为0.095~0.894。20个品系间遗传相似系数为0.328~0.891，基于UPGMA聚类可分为2个类群。所有15个位点均不能单独完全区分20个黑老虎品系，以KCZ023位点的区分能力最强；KCZ023与KCZ135、KCZ023与KCZ100以及KCZ023与KCZ147的2位点组合以及28个3位点组合能够将20个品系完全区分。20个黑老虎品系的遗传多样性较丰富，应用3个2位点组合和28个3位点组合能够完全区分各品系，该研究结果可为黑老虎种质鉴定以及杂交亲本选配提供科学依据。

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邹建文（1968—），男，本科，副研究员，研究方向：森林培育与植物资源开发利用。E-mail: zou5671695@126.com。

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邹建文（1968—），男，本科，副研究员，研究方向：森林培育与植物资源开发利用。E-mail: zou5671695@126.com。

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