Article(id=1236343707465732420, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.05.016, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1665417600000, receivedDateStr=2022-10-11, revisedDate=1672934400000, revisedDateStr=2023-01-06, acceptedDate=null, acceptedDateStr=null, onlineDate=1772696910014, onlineDateStr=2026-03-05, pubDate=1684944000000, pubDateStr=2023-05-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772696910014, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772696910014, creator=13701087609, updateTime=1772696910014, updator=13701087609, issue=Issue{id=1236343702042505492, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='5', pageStart='867', pageEnd='1082', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772696908722, creator=13701087609, updateTime=1772697049681, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236344293326115831, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236344293326115832, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1011, endPage=1022, ext={EN=ArticleExt(id=1236343707763528025, articleId=1236343707465732420, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Chemical Composition Analysis and in vitro Bacteriostasis Research of Cinnamomum camphora (L.) Presl Branches and Leaves From Diferent Habitats, columnId=1236292524264968282, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Protection & Bio-safety, runingTitle=null, highlight=null, articleAbstract=

C. camphora (L.) Presl is the most effective plant for extracting natural borneol in China. In order to help determine its proper habitat, this research plans to select branches and leaves of C. camphora (L.) Presl from eight habitats in China according to its habits, natural borneol of the leaves and branches of C. camphora (L.) Presl were extracted by steam distillation. The relative content of the chemical components in the extract was determined by GC-MS. The bacteriostatic circle diameter of Escherichia coli, Salmonella typhi and Salmonella enteritidis subsp was measured by the paper method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by 96 micro well plates. Chemical composition determination showed that the content of natural borneol extracted from the branches and leaves from different habitats was different, among them, d-borneol, camphor, d-limonene, eucalyptol, d-α-pinene, camphene, α-humulene and terpinen-4-ol were the common components, and the content of d-borneol was the highest, then camphor. The highest content of d-borneol was found from Yuan'an, Hubei, China (82.47%), and the lowest content was found from Ruyuan, Guangdong, China (41.63%). The natural borneol extracted from various habitats showed bacteriostatic effect on the three tested strains, and the diameter range of bacteriostatic ring was 14.60 to 27.95 mm, the minimum inhibitory concentration (MIC) ranged from 250 to 1250 μg/mL, the minimum bactericidal concentration (MBC) ranged from 0 to 1000 μg/mL. The natural borneol from Yuan'an and Jishui, Jiangxi had the best bacteriostatic effect, while that from Guangdong had the worst on the whole. In conclusion, the bacteriostatic activity of the extracts from the branches and leaves of C. camphora (L.) Presl is correlated with the content of its main components, and the habitat with the highest content of d-bornol has the best bacteriostatic effect, while the other has poor bacteriostatic effect. The data obtained in this research are accurate and reliable, and would provide a basis for the quality evaluation of the extracts of C. camphora (L.) Presl branches and leaves and the development of natural borneol resources.

, correspAuthors=Fulai YU, Yuxin PANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Siqi LI, Qian JIANG, Ying HUANG, Xuan HU, Kai WANG, Lingliang GUAN, Fulai YU, Yuxin PANG), CN=ArticleExt(id=1236343708019380585, articleId=1236343707465732420, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=不同产地龙脑樟枝叶化学成分分析及体外抑菌研究, columnId=1236292524520820846, journalTitle=热带作物学报, columnName=植物保护与生物安全, runingTitle=null, highlight=null, articleAbstract=

龙脑樟为目前国内提取天然冰片效益最高的植物,为助力其道地产区的确定,本研究根据龙脑樟的习性,拟选取国内8个产地的龙脑樟枝叶为材料,采用水蒸汽蒸馏法分别提取天然冰片;通过气质联用法(GC-MS)测定其中化学成分的相对含量;通过纸片法测定其对大肠杆菌、伤寒沙门氏菌以及肠炎沙门氏菌的抑菌圈直径;运用96微量孔板测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:各个产地龙脑樟枝叶提取的天然冰片中化合物含量具有差异性,其中以右旋龙脑、樟脑、d-柠檬烯、α-葎草烯、4-萜烯醇、桉叶油醇、莰烯以及d-α-蒎烯等为共有成分,且以右旋龙脑含量最高,樟脑次之,各产地中以湖北远安的右旋龙脑含量最高(82.47%),以广东乳源的右旋龙脑含量最低(41.63%);各个产地提取的天然冰片对3个供试菌种均有一定抑菌作用,且抑菌圈直径范围为14.60~27.95 mm;最低抑菌浓度(MIC)范围为250~1250 μg/mL;最低杀菌浓度(MBC)范围为0~1000 μg/mL,总体以湖北远安和江西吉水的综合抑菌效果最佳,广东乳源最差。由此可见,右旋龙脑含量最高的产地抑菌效果最佳,反之抑菌效果欠佳,因此,龙脑樟枝叶提取物的抑菌活性与其主要成分含量有一定相关性,本研究为龙脑樟枝叶提取物的品质评价及天然冰片的资源开发提供依据。

, correspAuthors=于福来, 庞玉新, authorNote=null, correspAuthorsNote=
* 庞玉新(PANGYuxin),E-mail:
于福来(YUFulai),E-mail:
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李思琪(1994—),女,硕士研究生,研究方向:中药资源开发与品质评价。

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2.中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口 571101, bio={"content":"

李思琪(1994—),女,硕士研究生,研究方向:中药资源开发与品质评价。

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李思琪(1994—),女,硕士研究生,研究方向:中药资源开发与品质评价。

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departmentName=null, remark=2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine (Haikou), Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Haikou, Hainan 571101, China), AuthorCompanyExt(id=1236343708329759099, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1236343707465732420, companyId=1236343708312981882, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口 571101)])]), Author(id=1236343709302837696, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1236343707465732420, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, 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不同产地龙脑樟枝叶化学成分分析及体外抑菌研究
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李思琪 1, 2 , 江芊 2 , 黄英 2 , 胡璇 2 , 王凯 2 , 官玲亮 2 , 于福来 2, * , 庞玉新 1, 3, *
热带作物学报 | 植物保护与生物安全 2023,44(5): 1011-1022
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热带作物学报 | 植物保护与生物安全 2023, 44(5): 1011-1022
不同产地龙脑樟枝叶化学成分分析及体外抑菌研究
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李思琪1, 2, 江芊2, 黄英2, 胡璇2, 王凯2, 官玲亮2, 于福来2, * , 庞玉新1, 3, *
作者信息
  • 1.广东药科大学中药资源学院,广东云浮 527300
  • 2.中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口 571101
  • 3.贵州中医药大学药学院,贵州贵阳 550025
  • 李思琪(1994—),女,硕士研究生,研究方向:中药资源开发与品质评价。

通讯作者:

* 庞玉新(PANGYuxin),E-mail:
于福来(YUFulai),E-mail:
Chemical Composition Analysis and in vitro Bacteriostasis Research of Cinnamomum camphora (L.) Presl Branches and Leaves From Diferent Habitats
Siqi LI1, 2, Qian JIANG2, Ying HUANG2, Xuan HU2, Kai WANG2, Lingliang GUAN2, Fulai YU2, * , Yuxin PANG1, 3, *
Affiliations
  • 1.School of Chinese Materia Medica Resources, Guangdong Pharmaceutical University, Yunfu, Guangdong 527300, China
  • 2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine (Haikou), Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Haikou, Hainan 571101, China
  • 3.School of Pharmacy, Guizhou University of Chinese Medicine, Guiyang, Guizhou 550025, China
出版时间: 2023-05-25 doi: 10.3969/j.issn.1000-2561.2023.05.016
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龙脑樟为目前国内提取天然冰片效益最高的植物,为助力其道地产区的确定,本研究根据龙脑樟的习性,拟选取国内8个产地的龙脑樟枝叶为材料,采用水蒸汽蒸馏法分别提取天然冰片;通过气质联用法(GC-MS)测定其中化学成分的相对含量;通过纸片法测定其对大肠杆菌、伤寒沙门氏菌以及肠炎沙门氏菌的抑菌圈直径;运用96微量孔板测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:各个产地龙脑樟枝叶提取的天然冰片中化合物含量具有差异性,其中以右旋龙脑、樟脑、d-柠檬烯、α-葎草烯、4-萜烯醇、桉叶油醇、莰烯以及d-α-蒎烯等为共有成分,且以右旋龙脑含量最高,樟脑次之,各产地中以湖北远安的右旋龙脑含量最高(82.47%),以广东乳源的右旋龙脑含量最低(41.63%);各个产地提取的天然冰片对3个供试菌种均有一定抑菌作用,且抑菌圈直径范围为14.60~27.95 mm;最低抑菌浓度(MIC)范围为250~1250 μg/mL;最低杀菌浓度(MBC)范围为0~1000 μg/mL,总体以湖北远安和江西吉水的综合抑菌效果最佳,广东乳源最差。由此可见,右旋龙脑含量最高的产地抑菌效果最佳,反之抑菌效果欠佳,因此,龙脑樟枝叶提取物的抑菌活性与其主要成分含量有一定相关性,本研究为龙脑樟枝叶提取物的品质评价及天然冰片的资源开发提供依据。

龙脑樟  /  右旋龙脑  /  相对含量  /  抑菌活性  /  天然冰片

C. camphora (L.) Presl is the most effective plant for extracting natural borneol in China. In order to help determine its proper habitat, this research plans to select branches and leaves of C. camphora (L.) Presl from eight habitats in China according to its habits, natural borneol of the leaves and branches of C. camphora (L.) Presl were extracted by steam distillation. The relative content of the chemical components in the extract was determined by GC-MS. The bacteriostatic circle diameter of Escherichia coli, Salmonella typhi and Salmonella enteritidis subsp was measured by the paper method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by 96 micro well plates. Chemical composition determination showed that the content of natural borneol extracted from the branches and leaves from different habitats was different, among them, d-borneol, camphor, d-limonene, eucalyptol, d-α-pinene, camphene, α-humulene and terpinen-4-ol were the common components, and the content of d-borneol was the highest, then camphor. The highest content of d-borneol was found from Yuan'an, Hubei, China (82.47%), and the lowest content was found from Ruyuan, Guangdong, China (41.63%). The natural borneol extracted from various habitats showed bacteriostatic effect on the three tested strains, and the diameter range of bacteriostatic ring was 14.60 to 27.95 mm, the minimum inhibitory concentration (MIC) ranged from 250 to 1250 μg/mL, the minimum bactericidal concentration (MBC) ranged from 0 to 1000 μg/mL. The natural borneol from Yuan'an and Jishui, Jiangxi had the best bacteriostatic effect, while that from Guangdong had the worst on the whole. In conclusion, the bacteriostatic activity of the extracts from the branches and leaves of C. camphora (L.) Presl is correlated with the content of its main components, and the habitat with the highest content of d-bornol has the best bacteriostatic effect, while the other has poor bacteriostatic effect. The data obtained in this research are accurate and reliable, and would provide a basis for the quality evaluation of the extracts of C. camphora (L.) Presl branches and leaves and the development of natural borneol resources.

C. camphora (L.) Presl  /  d-borneol  /  relative content  /  bacteriostatic activity  /  natural borneol
李思琪, 江芊, 黄英, 胡璇, 王凯, 官玲亮, 于福来, 庞玉新. 不同产地龙脑樟枝叶化学成分分析及体外抑菌研究. 热带作物学报, 2023 , 44 (5) : 1011 -1022 . DOI: 10.3969/j.issn.1000-2561.2023.05.016
Siqi LI, Qian JIANG, Ying HUANG, Xuan HU, Kai WANG, Lingliang GUAN, Fulai YU, Yuxin PANG. Chemical Composition Analysis and in vitro Bacteriostasis Research of Cinnamomum camphora (L.) Presl Branches and Leaves From Diferent Habitats[J]. Chinese Journal of Tropical Crops, 2023 , 44 (5) : 1011 -1022 . DOI: 10.3969/j.issn.1000-2561.2023.05.016
  • 广东省科研事业单位(省实验室分中心)重点领域研发计划项目(2021B0707010008)
  • 广东省重点领域研发计划项目(2020B020221002)
2023年第44卷第5期
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doi: 10.3969/j.issn.1000-2561.2023.05.016
  • 接收时间:2022-10-11
  • 首发时间:2026-03-05
  • 出版时间:2023-05-25
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  • 收稿日期:2022-10-11
  • 修回日期:2023-01-06
基金
广东省科研事业单位(省实验室分中心)重点领域研发计划项目(2021B0707010008)
广东省重点领域研发计划项目(2020B020221002)
作者信息
    1.广东药科大学中药资源学院,广东云浮 527300
    2.中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口 571101
    3.贵州中医药大学药学院,贵州贵阳 550025

通讯作者:

* 庞玉新(PANGYuxin),E-mail:
于福来(YUFulai),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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