Article(id=1236343704483582164, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.05.004, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1650729600000, receivedDateStr=2022-04-24, revisedDate=1658160000000, revisedDateStr=2022-07-19, acceptedDate=null, acceptedDateStr=null, onlineDate=1772696909304, onlineDateStr=2026-03-05, pubDate=1684944000000, pubDateStr=2023-05-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772696909304, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772696909304, creator=13701087609, updateTime=1772696909304, updator=13701087609, issue=Issue{id=1236343702042505492, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='5', pageStart='867', pageEnd='1082', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772696908722, creator=13701087609, updateTime=1772697049681, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236344293326115831, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236344293326115832, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236343702042505492, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=905, endPage=913, ext={EN=ArticleExt(id=1236343704710074583, articleId=1236343704483582164, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Analysis of the Whole Genome Sequence of Biocontrol Strain Bacillus velezensis HNU24, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

In order to reveal the genome characteristics of HNU24, which has excellent antagonistic ability to Ralstonia solanacearum, its complete genome was sequenced by the BGISEQ-500 and ONT PromethION technology, and the genome assembly, gene prediction, function annotation, and protein homology analysis also were conducted. Phylogenetic analysis results demonstrated that HNU24 belonged to Bacillus velezensis. Its completed genomic sequence length was 3 932 768 bp, GC content was 46.48%, and had 3822 genes. A total of 43 carbohydrate active enzyme gene families were identified in the genome, and the cellulase activity experiment further confirmed that the strain HNU24 had the ability to degrade cellulose. Thirteen clusters of secondary metabolite biosynthesis genes were predicted in genome, including eight compounds: surfactin, fengycin, plantazolicin, butirosin A/butirosin B, macrolactin, bacillaene, difficidin, bacillibactin and bacilysin, which have been reported as the antibacterial components. Five unknown components, which were found no homologous genes or were less similar to the known database sequences, may be new compounds that have not been identified and reported. In addition, greenhouse experiment demonstrated that HNU24 had significant growth promoting effect on tomato plants. Thus, HNU24 may antagonize R. solanacearum and promote tomato growth by secreting active compounds, and it is a potential biofertilizer strain. The relevant results would lay a foundation for further research and application of HNU24 in biological control.

, correspAuthors=Zhuangsheng WU, Peng LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Xiulan CAO, Yuting YE, Tianhao MA, Anna HU, Yu CAO, Pengze CHEN, Zhuangsheng WU, Peng LI), CN=ArticleExt(id=1236343704932372697, articleId=1236343704483582164, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=生防菌株贝莱斯芽孢杆菌HNU24全基因组测序及分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

本研究旨在解析对茄雷尔氏菌(Ralstonia solanacearum)具有较强拮抗活性的生防菌株HNU24的基因组特征。采用BGISEQ-500和ONT PromethION平台相结合的策略,对HNU24的全基因组进行了测序、组装、基因预测与功能注释、蛋白质同源分析。遗传进化分析结果表明:HNU24为贝莱斯芽孢杆菌(Bacillus velezensis),其完整基因组序列长度为3 932 768 bp,GC含量46.48%,编码3822个基因,其中有43个碳水化合物活性酶基因家族。纤维素酶活性实验进一步表明其具有分解纤维素的能力。在HNU24基因组中预测到13个次级代谢产物生物合成基因簇,其中包含表面活性素(surfactin)、泛革素(fengycin)、植物唑霉素(plantazolicin)、大环内酰亚胺(macrolactin)、杆菌烯(bacillaene)、地非西丁(difficidin)、杆菌毒素(bacillibactin)和杆溶菌素(bacilysin)8种已报道的对青枯菌等病原菌有拮抗活性的化合物。5个未能比对到同源基因或相似度较低,可能为未鉴定和报道的新化合物。此外,盆栽试验表明HNU24对番茄具有显著的促生长作用。综上,HNU24可能通过分泌活性化合物来拮抗茄雷尔氏菌并促进番茄生长,是一种潜在的生物肥料菌株,相关结果为进一步开展HNU24在生物防治方面的研究和应用奠定基础。

, correspAuthors=伍壮生, 李鹏, authorNote=null, correspAuthorsNote=
* 李鹏(LIPeng),E-mail:
伍壮生(WUZhuangsheng),E-mail:
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曹秀兰(1998—),女,硕士研究生,研究方向:修复生态学。

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曹秀兰(1998—),女,硕士研究生,研究方向:修复生态学。

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曹秀兰(1998—),女,硕士研究生,研究方向:修复生态学。

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曹秀兰 1 , 叶雨婷 1 , 马天浩 1 , 胡安娜 1 , 曹宇 1 , 陈鹏泽 1 , 伍壮生 2, * , 李鹏 1, *
热带作物学报 | 组学与生物技术 2023,44(5): 905-913
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热带作物学报 | 组学与生物技术 2023, 44(5): 905-913
生防菌株贝莱斯芽孢杆菌HNU24全基因组测序及分析
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曹秀兰1, 叶雨婷1, 马天浩1, 胡安娜1, 曹宇1, 陈鹏泽1, 伍壮生2, * , 李鹏1, *
作者信息
  • 1.海南师范大学/热带岛屿生态学教育部重点实验室/海南省热带动植物生态学重点实验室,海南海口 571158
  • 2.海南省农业科学院蔬菜研究所,海南海口 571100
  • 曹秀兰(1998—),女,硕士研究生,研究方向:修复生态学。

通讯作者:

* 李鹏(LIPeng),E-mail:
伍壮生(WUZhuangsheng),E-mail:
Analysis of the Whole Genome Sequence of Biocontrol Strain Bacillus velezensis HNU24
Xiulan CAO1, Yuting YE1, Tianhao MA1, Anna HU1, Yu CAO1, Pengze CHEN1, Zhuangsheng WU2, * , Peng LI1, *
Affiliations
  • 1.Hainan Normal University/Key Laboratory of Ministry of Education for Ecology of Tropical Islands/Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Haikou, Hainan 571158, China
  • 2.Vegetable Research Institute, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100, China
出版时间: 2023-05-25 doi: 10.3969/j.issn.1000-2561.2023.05.004
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本研究旨在解析对茄雷尔氏菌(Ralstonia solanacearum)具有较强拮抗活性的生防菌株HNU24的基因组特征。采用BGISEQ-500和ONT PromethION平台相结合的策略,对HNU24的全基因组进行了测序、组装、基因预测与功能注释、蛋白质同源分析。遗传进化分析结果表明:HNU24为贝莱斯芽孢杆菌(Bacillus velezensis),其完整基因组序列长度为3 932 768 bp,GC含量46.48%,编码3822个基因,其中有43个碳水化合物活性酶基因家族。纤维素酶活性实验进一步表明其具有分解纤维素的能力。在HNU24基因组中预测到13个次级代谢产物生物合成基因簇,其中包含表面活性素(surfactin)、泛革素(fengycin)、植物唑霉素(plantazolicin)、大环内酰亚胺(macrolactin)、杆菌烯(bacillaene)、地非西丁(difficidin)、杆菌毒素(bacillibactin)和杆溶菌素(bacilysin)8种已报道的对青枯菌等病原菌有拮抗活性的化合物。5个未能比对到同源基因或相似度较低,可能为未鉴定和报道的新化合物。此外,盆栽试验表明HNU24对番茄具有显著的促生长作用。综上,HNU24可能通过分泌活性化合物来拮抗茄雷尔氏菌并促进番茄生长,是一种潜在的生物肥料菌株,相关结果为进一步开展HNU24在生物防治方面的研究和应用奠定基础。

贝莱斯芽孢杆菌  /  番茄青枯病  /  全基因组  /  次级代谢产物

In order to reveal the genome characteristics of HNU24, which has excellent antagonistic ability to Ralstonia solanacearum, its complete genome was sequenced by the BGISEQ-500 and ONT PromethION technology, and the genome assembly, gene prediction, function annotation, and protein homology analysis also were conducted. Phylogenetic analysis results demonstrated that HNU24 belonged to Bacillus velezensis. Its completed genomic sequence length was 3 932 768 bp, GC content was 46.48%, and had 3822 genes. A total of 43 carbohydrate active enzyme gene families were identified in the genome, and the cellulase activity experiment further confirmed that the strain HNU24 had the ability to degrade cellulose. Thirteen clusters of secondary metabolite biosynthesis genes were predicted in genome, including eight compounds: surfactin, fengycin, plantazolicin, butirosin A/butirosin B, macrolactin, bacillaene, difficidin, bacillibactin and bacilysin, which have been reported as the antibacterial components. Five unknown components, which were found no homologous genes or were less similar to the known database sequences, may be new compounds that have not been identified and reported. In addition, greenhouse experiment demonstrated that HNU24 had significant growth promoting effect on tomato plants. Thus, HNU24 may antagonize R. solanacearum and promote tomato growth by secreting active compounds, and it is a potential biofertilizer strain. The relevant results would lay a foundation for further research and application of HNU24 in biological control.

Bacillus velezensis  /  tomato bacterial wilt  /  complete genome  /  secondary metabolites
曹秀兰, 叶雨婷, 马天浩, 胡安娜, 曹宇, 陈鹏泽, 伍壮生, 李鹏. 生防菌株贝莱斯芽孢杆菌HNU24全基因组测序及分析. 热带作物学报, 2023 , 44 (5) : 905 -913 . DOI: 10.3969/j.issn.1000-2561.2023.05.004
Xiulan CAO, Yuting YE, Tianhao MA, Anna HU, Yu CAO, Pengze CHEN, Zhuangsheng WU, Peng LI. Analysis of the Whole Genome Sequence of Biocontrol Strain Bacillus velezensis HNU24[J]. Chinese Journal of Tropical Crops, 2023 , 44 (5) : 905 -913 . DOI: 10.3969/j.issn.1000-2561.2023.05.004
  • 海南省重点研发计划项目(ZDYF2020080)
2023年第44卷第5期
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doi: 10.3969/j.issn.1000-2561.2023.05.004
  • 接收时间:2022-04-24
  • 首发时间:2026-03-05
  • 出版时间:2023-05-25
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  • 收稿日期:2022-04-24
  • 修回日期:2022-07-19
基金
海南省重点研发计划项目(ZDYF2020080)
作者信息
    1.海南师范大学/热带岛屿生态学教育部重点实验室/海南省热带动植物生态学重点实验室,海南海口 571158
    2.海南省农业科学院蔬菜研究所,海南海口 571100

通讯作者:

* 李鹏(LIPeng),E-mail:
伍壮生(WUZhuangsheng),E-mail:
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.05.004
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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