Article(id=1236337188602966659, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.06.010, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1655136000000, receivedDateStr=2022-06-14, revisedDate=1661875200000, revisedDateStr=2022-08-31, acceptedDate=null, acceptedDateStr=null, onlineDate=1772695355796, onlineDateStr=2026-03-05, pubDate=1687622400000, pubDateStr=2023-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772695355796, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772695355796, creator=13701087609, updateTime=1772695355796, updator=13701087609, issue=Issue{id=1236337182303113996, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='6', pageStart='1083', pageEnd='1305', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772695354295, creator=13701087609, updateTime=1772695447813, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236337574608950114, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236337574613144419, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1161, endPage=1169, ext={EN=ArticleExt(id=1236337188913345174, articleId=1236337188602966659, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Effects of Different Hormones and Trace Elements on Increasing Aroma of Pandanus amaryllifolius Roxb. Plantlets in vitro, columnId=1236256434120348225, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Cultivation, Physiology & Biochemistry, runingTitle=null, highlight=null, articleAbstract=

In this experiment, Pandanus amaryllifolius Roxb. plantlets in vitro were used as detected material. In order to investigate the most suitable fertilization formula for increasing aroma of P. amaryllifolius Roxb. plantlets in vitro. And the volatile components of P. amaryllifolius Roxb. plantlets in vitro were separated and identified by gas chromatography-mass spectrometry (GC-MS), and the results were analyzed by principal component analysis (PCA) and cluster heatmap analysis. The results showed that the volatile components were similar in seven different treatments, but their volatile contents were different greatly (P<0.05). A total of 21 volatile components were detected, including alcohols, pyrroles, esters, ketones, furans, furans, acids, olefins and phenols, and squalene, phytol. Among them 2-acetyl-1-pyrroline (2AP), neophytadiene, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran were the main volatile components. The average content of the characteristic aroma component 2AP were (48.07±13.14)μg/g of P. amaryllifolius Roxb. plantlets in vitro, and the treatment 6 (zinc+fertilizer combinations) was hightest to 72.03 μg/g; however the treatment 1 (IBA+zinc+fertilizer combinations) was the lowest to 31.96 μg/g, and the treatment 6 could significantly increase the content of the characteristic aroma component 2AP. The results of principal component analysis showed significant difference between the fertilizer combinations treatment (ammonium bicarbonate + calcium superphosphate+potassium chloride) and the water treatment, and the main volatile components of the treatment 2 and the treatment 6 were phytol, 2AP, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran, neophytadiene; and the main volatile components of the treatment 1 and the treatment 5 were ethyl oleate-based esters, 2,4-di-tert-butylphenol-based phenols, acetol-based ketones. The volatile components were mainly squalene in the treatment 3, treatment 4 and treatment 7. Cluster analysis showed that higher volatile component in the treatment 6 (zinc+fertilizer combinations), and the volatile component were significantly lower in the treatment 1 (IBA+zinc+fertilizer combinations). There were no obviously differences in other treated groups. The conclusion indicated that zinc sulfate immersion and ammonium bicarbonate+calcium superphosphate+potassium chloride fertilizer can improve aroma than other groups.

, correspAuthors=Xiaowei QIN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Youli LI, Xunzhi JI, Xiaowei QIN, Huan YU, Ang ZHANG, Ying ZONG, Shuzhen HE), CN=ArticleExt(id=1236337189156614826, articleId=1236337188602966659, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=不同激素及微量元素处理对斑兰叶组培苗增香的影响, columnId=1236256434313286224, journalTitle=热带作物学报, columnName=作物栽培与生理生化, runingTitle=null, highlight=null, articleAbstract=

以斑兰叶组培苗为材料,为探明最适宜斑兰叶增香的施肥配方,研究不同激素、微量元素对斑兰叶组培苗增香的影响。采用气相色谱-质谱联用技术(GC-MS)对斑兰叶的挥发性香气成分进行鉴定分析,并对结果进行主成分分析及聚类热图分析。结果显示:7个不同处理的斑兰叶组培苗挥发性成分种类组成无显著差异,但挥发性成分含量差异显著(P<0.05)。共分析鉴定出21种化合物,主要由醇类、吡咯类、酯类、酮类、呋喃类、呋喃酮类、酸类、烯烃类、酚类组成,其中角鲨烯、叶绿醇、2-乙酰-1-吡咯啉(2AP)、新植二烯、3-甲基-2-(5H)-呋喃酮、2,3-二氢苯并呋喃为主要香气挥发性成分。斑兰叶组培苗特征香气成分2AP平均含量为(48.07±13.14)μg/g,其中处理6(锌+组合肥)的2AP含量最高,为72.03 μg/g;处理1(IBA+锌+组合肥)2AP含量最低,为31.96 μg/g,表明处理6能显著提高特征性物质2AP的含量。主成分分析表明,施用组合肥(碳酸氢铵+过磷酸钙+氯化钾)与施用清水存在较大差异,并且处理2、处理6中主要挥发性成分为叶绿醇、2AP、3-甲基-2(5H)-呋喃酮、2,3-二氢苯并呋喃、新植二烯;处理1、处理5中挥发性成分主要以油酸乙酯为主的酯类、2,4-叔丁基苯酚为主的酚类、丙酮醇为主的酮类等化合物;处理3、处理4、处理7中挥发性物质主要以角鲨烯为主。通过聚类分析发现,处理6(锌+组合肥)中斑兰叶组培苗的挥发性香气成分均有较高的积累;而处理1(IBA+锌+组合肥)中挥发性物质含量较少;其余处理对斑兰叶组培苗主要挥发性香气成分的影响较小。研究表明:采用硫酸锌浸泡和配施碳酸氢铵+过磷酸钙+氯化钾溶液优于其他处理组,有较好的增香效果。

, correspAuthors=秦晓威, authorNote=null, correspAuthorsNote=
* 秦晓威(QINXiaowei),E-mail:
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李有丽(2000—),女,本科生,研究方向:热带作物组织培养。

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5.云南农业大学热带作物学院,云南普洱 665000, bio={"content":"

李有丽(2000—),女,本科生,研究方向:热带作物组织培养。

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李有丽(2000—),女,本科生,研究方向:热带作物组织培养。

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2.Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
3.Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
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2.农业农村部香辛饮料作物遗传资源利用重点实验室,海南万宁 5715333
3.海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
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2.Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
3.Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
4.Hainan Provincial Sim Soonliang Academician Workstation, Wanning, Hainan 571533, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1236337190905639723, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1236337188602966659, authorId=1236337190683341590, language=CN, stringName=秦晓威, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, 4, *, address=1.中国热带农业科学院香料饮料研究所,海南万宁 571533
2.农业农村部香辛饮料作物遗传资源利用重点实验室,海南万宁 5715333
3.海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
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不同激素及微量元素处理对斑兰叶组培苗增香的影响
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李有丽 1, 5 , 吉训志 1, 2, 3, 4 , 秦晓威 1, 2, 3, 4, * , 鱼欢 1, 2, 3 , 张昂 1, 2, 3 , 宗迎 1, 2, 3 , 贺书珍 1, 2, 3
热带作物学报 | 作物栽培与生理生化 2023,44(6): 1161-1169
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热带作物学报 | 作物栽培与生理生化 2023, 44(6): 1161-1169
不同激素及微量元素处理对斑兰叶组培苗增香的影响
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李有丽1, 5, 吉训志1, 2, 3, 4, 秦晓威1, 2, 3, 4, * , 鱼欢1, 2, 3, 张昂1, 2, 3, 宗迎1, 2, 3, 贺书珍1, 2, 3
作者信息
  • 1.中国热带农业科学院香料饮料研究所,海南万宁 571533
  • 2.农业农村部香辛饮料作物遗传资源利用重点实验室,海南万宁 5715333
  • 3.海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
  • 4.海南省Sim Soonliang院士工作站,海南万宁 571533
  • 5.云南农业大学热带作物学院,云南普洱 665000
  • 李有丽(2000—),女,本科生,研究方向:热带作物组织培养。

通讯作者:

* 秦晓威(QINXiaowei),E-mail:
Effects of Different Hormones and Trace Elements on Increasing Aroma of Pandanus amaryllifolius Roxb. Plantlets in vitro
Youli LI1, 5, Xunzhi JI1, 2, 3, 4, Xiaowei QIN1, 2, 3, 4, * , Huan YU1, 2, 3, Ang ZHANG1, 2, 3, Ying ZONG1, 2, 3, Shuzhen HE1, 2, 3
Affiliations
  • 1.Spice and Beverage of Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China
  • 2.Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture and Rural Affairs, Wanning, Hainan 571533, China
  • 3.Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China
  • 4.Hainan Provincial Sim Soonliang Academician Workstation, Wanning, Hainan 571533, China
  • 5.Tropical Crop College, Yunnan Agricultural University, Pu'er, Yunnan 665000, China
出版时间: 2023-06-25 doi: 10.3969/j.issn.1000-2561.2023.06.010
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以斑兰叶组培苗为材料,为探明最适宜斑兰叶增香的施肥配方,研究不同激素、微量元素对斑兰叶组培苗增香的影响。采用气相色谱-质谱联用技术(GC-MS)对斑兰叶的挥发性香气成分进行鉴定分析,并对结果进行主成分分析及聚类热图分析。结果显示:7个不同处理的斑兰叶组培苗挥发性成分种类组成无显著差异,但挥发性成分含量差异显著(P<0.05)。共分析鉴定出21种化合物,主要由醇类、吡咯类、酯类、酮类、呋喃类、呋喃酮类、酸类、烯烃类、酚类组成,其中角鲨烯、叶绿醇、2-乙酰-1-吡咯啉(2AP)、新植二烯、3-甲基-2-(5H)-呋喃酮、2,3-二氢苯并呋喃为主要香气挥发性成分。斑兰叶组培苗特征香气成分2AP平均含量为(48.07±13.14)μg/g,其中处理6(锌+组合肥)的2AP含量最高,为72.03 μg/g;处理1(IBA+锌+组合肥)2AP含量最低,为31.96 μg/g,表明处理6能显著提高特征性物质2AP的含量。主成分分析表明,施用组合肥(碳酸氢铵+过磷酸钙+氯化钾)与施用清水存在较大差异,并且处理2、处理6中主要挥发性成分为叶绿醇、2AP、3-甲基-2(5H)-呋喃酮、2,3-二氢苯并呋喃、新植二烯;处理1、处理5中挥发性成分主要以油酸乙酯为主的酯类、2,4-叔丁基苯酚为主的酚类、丙酮醇为主的酮类等化合物;处理3、处理4、处理7中挥发性物质主要以角鲨烯为主。通过聚类分析发现,处理6(锌+组合肥)中斑兰叶组培苗的挥发性香气成分均有较高的积累;而处理1(IBA+锌+组合肥)中挥发性物质含量较少;其余处理对斑兰叶组培苗主要挥发性香气成分的影响较小。研究表明:采用硫酸锌浸泡和配施碳酸氢铵+过磷酸钙+氯化钾溶液优于其他处理组,有较好的增香效果。

斑兰叶  /  挥发性成分  /  2-乙酰-1-吡咯啉  /  激素  /  微量元素

In this experiment, Pandanus amaryllifolius Roxb. plantlets in vitro were used as detected material. In order to investigate the most suitable fertilization formula for increasing aroma of P. amaryllifolius Roxb. plantlets in vitro. And the volatile components of P. amaryllifolius Roxb. plantlets in vitro were separated and identified by gas chromatography-mass spectrometry (GC-MS), and the results were analyzed by principal component analysis (PCA) and cluster heatmap analysis. The results showed that the volatile components were similar in seven different treatments, but their volatile contents were different greatly (P<0.05). A total of 21 volatile components were detected, including alcohols, pyrroles, esters, ketones, furans, furans, acids, olefins and phenols, and squalene, phytol. Among them 2-acetyl-1-pyrroline (2AP), neophytadiene, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran were the main volatile components. The average content of the characteristic aroma component 2AP were (48.07±13.14)μg/g of P. amaryllifolius Roxb. plantlets in vitro, and the treatment 6 (zinc+fertilizer combinations) was hightest to 72.03 μg/g; however the treatment 1 (IBA+zinc+fertilizer combinations) was the lowest to 31.96 μg/g, and the treatment 6 could significantly increase the content of the characteristic aroma component 2AP. The results of principal component analysis showed significant difference between the fertilizer combinations treatment (ammonium bicarbonate + calcium superphosphate+potassium chloride) and the water treatment, and the main volatile components of the treatment 2 and the treatment 6 were phytol, 2AP, 3-methyl-2-(5H)-furanone, 2,3-dihydrobenzofuran, neophytadiene; and the main volatile components of the treatment 1 and the treatment 5 were ethyl oleate-based esters, 2,4-di-tert-butylphenol-based phenols, acetol-based ketones. The volatile components were mainly squalene in the treatment 3, treatment 4 and treatment 7. Cluster analysis showed that higher volatile component in the treatment 6 (zinc+fertilizer combinations), and the volatile component were significantly lower in the treatment 1 (IBA+zinc+fertilizer combinations). There were no obviously differences in other treated groups. The conclusion indicated that zinc sulfate immersion and ammonium bicarbonate+calcium superphosphate+potassium chloride fertilizer can improve aroma than other groups.

Pandanus amaryllifolius Roxb.  /  volatile components  /  2-acetyl-1-pyrroline  /  hormones  /  trace elements
李有丽, 吉训志, 秦晓威, 鱼欢, 张昂, 宗迎, 贺书珍. 不同激素及微量元素处理对斑兰叶组培苗增香的影响. 热带作物学报, 2023 , 44 (6) : 1161 -1169 . DOI: 10.3969/j.issn.1000-2561.2023.06.010
Youli LI, Xunzhi JI, Xiaowei QIN, Huan YU, Ang ZHANG, Ying ZONG, Shuzhen HE. Effects of Different Hormones and Trace Elements on Increasing Aroma of Pandanus amaryllifolius Roxb. Plantlets in vitro[J]. Chinese Journal of Tropical Crops, 2023 , 44 (6) : 1161 -1169 . DOI: 10.3969/j.issn.1000-2561.2023.06.010
  • 海南省自然科学基金青年基金项目(322QN403)
  • 科技部、财政部国家科技资源共享服务平台(NPGRC 2022-014)
2023年第44卷第6期
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doi: 10.3969/j.issn.1000-2561.2023.06.010
  • 接收时间:2022-06-14
  • 首发时间:2026-03-05
  • 出版时间:2023-06-25
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  • 收稿日期:2022-06-14
  • 修回日期:2022-08-31
基金
海南省自然科学基金青年基金项目(322QN403)
科技部、财政部国家科技资源共享服务平台(NPGRC 2022-014)
作者信息
    1.中国热带农业科学院香料饮料研究所,海南万宁 571533
    2.农业农村部香辛饮料作物遗传资源利用重点实验室,海南万宁 5715333
    3.海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁 571533
    4.海南省Sim Soonliang院士工作站,海南万宁 571533
    5.云南农业大学热带作物学院,云南普洱 665000

通讯作者:

* 秦晓威(QINXiaowei),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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