Article(id=1236337188141593200, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.06.018, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1663084800000, receivedDateStr=2022-09-14, revisedDate=1666627200000, revisedDateStr=2022-10-25, acceptedDate=null, acceptedDateStr=null, onlineDate=1772695355687, onlineDateStr=2026-03-05, pubDate=1687622400000, pubDateStr=2023-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772695355687, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772695355687, creator=13701087609, updateTime=1772695355687, updator=13701087609, issue=Issue{id=1236337182303113996, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='6', pageStart='1083', pageEnd='1305', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772695354295, creator=13701087609, updateTime=1772695447813, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236337574608950114, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236337574613144419, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236337182303113996, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1246, endPage=1254, ext={EN=ArticleExt(id=1236337188435194491, articleId=1236337188141593200, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Isolation, Identification and Biological Activities of Chemical Constituents from the Fruiting Body of Fuscoporia punctata (Fr.) Cunn, columnId=1236286112713470633, journalTitle=Chinese Journal of Tropical Crops, columnName=Post-harvest Treatment & Quality Safety, runingTitle=null, highlight=null, articleAbstract=

The constituents of the fruiting body of Fuscoporia punctata (Fr.) Cunn were isolated and purified by different column chromatography and HPLC method to study the the pharmacological basis, chemical constituents and biological activity. The structures of the compounds were identified by spectral data analysis including-NMR and MS. The inhibitory activity of α-glucosidase and protein tyrosine phosphatase 1B (PTP1B) for the compounds were evaluated by PNPG and pNPP methods, respectively. The DPPH scavenging activity of the compounds was determined by the DPPH method. The results preliminarily identified the main chemical composition types of F. punctata (Fr.) Cunn. 12 compounds, including 7 polyketides, were isolated from the EtOH extract of the fruiting body of F. punctata and were identified as inoscavin A (1), inoscavin C (2), phellibaumin A (3), phellibaumin D (4), inonotusin B (5), phellifuropyranone A (6), phelligridin D (7), protocatechualdehyde (8), catechol (9), pyrogallol (10), p-hydroxybenzoic acid (11), and 4-hydroxybenzylideneacetone (12). Compounds 4, 5 and 7 had definite PTP1B inhibitory activity with the IC₅₀ values of (60.83±2.01), (56.33±1.57), (66.89±0.96) μmol/L, respectively. Compounds 2 and 7 exhibited inhibitory activity againstα-glucosidase with the IC₅₀ values of (127.77±0.56), (122.99±1.39) μmol/L, respectively. The IC₅₀ value of compounds 4, 7, 2 and 6 scavenging DPPH radical was (6.71±0.69), (25.79±1.59), (30.45±1.67), (32.90±1.59) μmol/L, respectively. All the compounds were obtained from these fungi for the first time. Most of the polyketides had PTP1B inhibitory activity, α-glycosidase inhibitory activity and DPPH scavenging activity, which would provide theoretical basis for the further development and utilization of F. punctata (Fr.) Cunn.

, correspAuthors=Youxing ZHAO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Xiaopeng WU, Ran LI, Qingyun MA, Li YANG, Qingyi XIE, Haofu DAI, Youxing ZHAO), CN=ArticleExt(id=1236337188703629961, articleId=1236337188141593200, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=斑褐孔菌子实体化学成分的分离鉴定及生物活性研究, columnId=1236286112877048492, journalTitle=热带作物学报, columnName=采后处理与质量安全, runingTitle=null, highlight=null, articleAbstract=

为了研究斑褐孔菌（Fuscoporia punctata）子实体的药效物质基础、化学成分及生物活性，采用各种柱色谱与高效液相色谱相结合的方法进行分离纯化，采用核磁共振和质谱等方法鉴定化合物的结构；采用PNPG法、pNPP法和DPPH法分别测定化合物对α-糖苷酶、蛋白酪氨酸磷酸酶1B（PTP1B）的抑制活性和清除DPPH自由基的活性。研究结果初步明确了海南斑褐孔菌的主要化学成分类型，从斑褐孔菌子实体的乙醇提取物中分离得到12个化合物，分别为inoscavin A（1）、inoscavin C（2）、phellibaumin A（3）、phellibaumin D（4）、inonotusin B（5）、phellifuropyranone A（6）、phelligridin D（7）、原儿茶醛（8）、儿茶酚（9）、没食子酚（10）、对羟基苯甲酸（11）和对羟基苄叉丙酮（12），其中化合物1~7为聚酮类成分，化合物4、5、7抑制PTP1B的IC₅₀值分别为（60.83±2.01）、（56.33±1.57）、（66.89±0.96）μmol/L，化合物2、7抑制α-糖苷酶的IC₅₀值分别为（127.77±0.56）、（122.99±1.39）μmol/L，化合物4、7、2、6清除DPPH自由基的IC₅₀值分别为（6.71±0.69）、（25.79±1.59）、（30.45±1.67）、（32.90±1.59）μmol/L。所有化合物均为首次从斑褐孔菌中分离得到，大部分的聚酮类成分具有PTP1B抑制活性、α-糖苷酶抑制活性和清除DPPH活性，研究结果为海南斑褐孔菌的深度开发利用提供理论依据。

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^2.中国热带农业科学院热带生物技术研究所/海口市热带天然产物研究与利用重点实验室/海南热带农业资源研究院/海南省热带农业生物资源保护与利用重点实验室，海南海口　571101, bio={"content":"

吴晓鹏（1981—），男，硕士，高级实验师，研究方向：检测技术及精密仪器。

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吴晓鹏（1981—），男，硕士，高级实验师，研究方向：检测技术及精密仪器。

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