热带作物学报

基于iPBS标记的石斛兰杂交子代真实性鉴定

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赵秋菊 ¹^,²^,³ , 崔学强 ³ , 邓杰玲 ³ , 黄昌艳 ³ , 李佳蔚 ¹^,²^,^* , 张自斌 ³^,^*

热带作物学报 | 种质资源与遗传育种 2023,44(6): 1153-1160

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热带作物学报 | 种质资源与遗传育种 2023, 44(6): 1153-1160

基于iPBS标记的石斛兰杂交子代真实性鉴定

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赵秋菊¹^,²^,³, 崔学强³, 邓杰玲³, 黄昌艳³, 李佳蔚¹^,²^,^*, 张自斌³^,^*

作者信息

^1.广西大学亚热带农业生物资源保护与利用国家重点实验室，广西南宁　530004

^2.广西大学林学院广西森林生态与保育重点实验室，广西南宁　530004

^3.广西壮族自治区农业科学院花卉研究所，广西南宁　530007

赵秋菊（1999—），女，硕士研究生，研究方向：兰科植物分子育种。

通讯作者:

^* 李佳蔚（LIJiawei），E-mail: lijiawei1662020@163.com。

张自斌（ZHANGZibin），E-mail: candou154@126.com。

Authenticity Identification of Hybrid Progenies of Dendrobium Based on iPBS Markers

Qiuju ZHAO¹^,²^,³, Xueqiang CUI³, Jieling DENG³, Changyan HUANG³, Jiawei LI¹^,²^,^*, Zibin ZHANG³^,^*

Affiliations

^1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi 530004, China

^2.Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning, Guangxi 530004, China

^3.Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China

出版时间: 2023-06-25 doi: 10.3969/j.issn.1000-2561.2023.06.009

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摘要

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石斛兰为石斛属所有植物的总称，是兰科植物中重要的花卉资源，具有极高的观赏和药用价值。杂交育种是培育石斛兰新品种的主要方法，但石斛兰的育种年限较长，整个育种周期通常需要几年的时间。种质资源的鉴定是育种工作的基础，本研究对石斛兰杂交后代株系进行鉴定，以期为石斛兰杂种后代的早期选择及新品种（系）的快速鉴定提供分子依据，进一步缩短育种周期，加快石斛兰育种工作进程。iPBS标记作为一种新型的分子标记，无需预先获取LTR序列，相较于其他分子标记具有简单、高效且应用范围广等优点，本研究采用iPBS分子标记技术对广美石斛×黄钻戒石斛的19个杂种F₁株系进行杂种真实性鉴定，并进行遗传多样性和聚类分析。研究结果表明：从83条iPBS引物中筛选出7条扩增条带清晰、多态性高、重复性好的引物用于石斛兰杂交子代真实性鉴定；7条引物共扩增出69条条带，其中多态性条带为51条，多态性比率为73.91%；较多的父本遗传信息导入子代个体中，供试的19个杂种F₁株系均能扩增出父本特征性条带，鉴定为真杂种；聚类分析表明，绝大多数杂交后代在亲缘关系上先倾向于父本后倾向于母本；遗传相似系数分析结果表明，杂交后代与父本的平均遗传相似系数大于母本，说明供试杂交后代在遗传上更倾向于父本，与聚类结果吻合；该杂交组合子代植株偏父本遗传。研究结果证明，iPBS分子标记技术可作为石斛兰杂交育种早期杂交种筛选鉴定的有效方法。该研究结果为进一步揭示石斛兰种群亲本与子代间的遗传多样性提供科学依据。

关键词

石斛兰 / iPBS标记 / 杂交子代 / 真实性鉴定

Abstract

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Dendrobium is the general name of all plants in Genus Dendrobium Sw. It is an important flower resource in orchid plants and has high ornamental and medicinal value. Crossbreeding is the main method to cultivate new varieties of Dendrobium, However, the breeding period of Dendrobium is long, and the whole breeding cycle usually takes several years. The identification of germplasm resources is the basis of breeding work. This study of the identification of the hybrid progeny lines of Dendrobium is expected to provide molecular basis for early selection of hybrid progeny and rapid identification of new varieties (lines), shorten the breeding cycle and accelerate the progress of Dendrobium breeding. As a new type of molecular marker, iPBS marker does not need to obtain the LTR sequence in advance. Compared with other molecular markers, it has the advantages of simplicity, efficiency and wide application. In the study, the authenticity of 19 hybrid F₁ lines of Dendrobium Pittero Gold 'Diamond Ring' × Dendrobium Btilliant Smile 'Hiromi' was identified by iPBS molecular marker technology, and perform genetic diversity and cluster analysis. Seven primers with clear amplification bands, high polymorphism and good repeatability were selected from 83 iPBS primers for the authenticity identification of hybrid progenies of Dendrobium. A total of 69 bands were amplified by the seven primers, of which 51 were polymorphic, and the polymorphic ratio was 73.91%. The 19 F₁ hybrid lines tested could amplify paternal characteristic bands and were identified as true hybrids. A large number of paternal characteristic bands were amplified in the offspring plants, indicating that more genetic information of Dendrobium Pittero Gold 'Diamond Ring' was introduced into the offspring individuals, showing obvious paternal inheritance. Cluster analysis showed that the vast majority of the hybrid offspring tended to be paternal first and then maternal. The results of genetic similarity coefficient analysis showed that the average genetic similarity coefficient between the hybrid offspring and the male parent was greater than that of the female parent, indicating that the tested hybrid offspring were more inclined to the male parent in heredity, which was consistent with the clustering results. The variation of genetic similarity coefficient between the two parents and the offspring was small, indicating that the genetic diversity between the offspring of the hybrid combination was not rich. This is not conducive to the emergence of heterosis varieties, in the subsequent experiments, plants with large genetic differences should be selected for hybrid breeding in order to obtain heterosis varieties. The results showed that the iPBS molecular marker technology could be used as an effective method for the screening and identification of early hybrids in Dendrobium cross breeding. The results of this study would provide a scientific basis for further revealing the genetic diversity between parents and progeny of Dendrobium population.

Key words

Dendrobium / iPBS marker / hybrid progeny / authenticity identification

引用本文

赵秋菊, 崔学强, 邓杰玲, 黄昌艳, 李佳蔚, 张自斌. 基于iPBS标记的石斛兰杂交子代真实性鉴定. 热带作物学报, 2023 , 44 (6) : 1153 -1160 . DOI: 10.3969/j.issn.1000-2561.2023.06.009

Qiuju ZHAO, Xueqiang CUI, Jieling DENG, Changyan HUANG, Jiawei LI, Zibin ZHANG. Authenticity Identification of Hybrid Progenies of Dendrobium Based on iPBS Markers[J]. Chinese Journal of Tropical Crops, 2023 , 44 (6) : 1153 -1160 . DOI: 10.3969/j.issn.1000-2561.2023.06.009

基金

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广西自然科学基金项目(2021GXNSFBA075059)
广西自然科学基金项目(2020GXNSFBA297021)
广西重点研发计划项目(桂科AB21220056)

参考文献

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2023年第44卷第6期

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文章信息

doi: 10.3969/j.issn.1000-2561.2023.06.009

接收时间：2022-07-01
首发时间：2026-03-05
出版时间：2023-06-25

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出版历史

收稿日期：2022-07-01
修回日期：2022-08-18

基金

广西自然科学基金项目(2021GXNSFBA075059)

广西自然科学基金项目(2020GXNSFBA297021)

广西重点研发计划项目(桂科AB21220056)

作者信息

^1.广西大学亚热带农业生物资源保护与利用国家重点实验室，广西南宁　530004

^2.广西大学林学院广西森林生态与保育重点实验室，广西南宁　530004

^3.广西壮族自治区农业科学院花卉研究所，广西南宁　530007

通讯作者:

^* 李佳蔚（LIJiawei），E-mail: lijiawei1662020@163.com。

张自斌（ZHANGZibin），E-mail: candou154@126.com。

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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.06.009

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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