Article(id=1236324892514242677, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236324890941378645, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.12.015, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1661097600000, receivedDateStr=2022-08-22, revisedDate=1666022400000, revisedDateStr=2022-10-18, acceptedDate=null, acceptedDateStr=null, onlineDate=1772692424180, onlineDateStr=2026-03-05, pubDate=1703433600000, pubDateStr=2023-12-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772692424180, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772692424180, creator=13701087609, updateTime=1772692424180, updator=13701087609, issue=Issue{id=1236324890941378645, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='12', pageStart='2355', pageEnd='2578', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772692423806, creator=13701087609, updateTime=1772693190259, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236328105745380010, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236324890941378645, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236328105749574315, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236324890941378645, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=2498, endPage=2505, ext={EN=ArticleExt(id=1236324892837204097, articleId=1236324892514242677, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Analysis of Genetic Diversity and Evaluation of Heat Resistance of Anoectochilus roburghii, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Anoectochilus roburghii is a rare and endangered medicinal orchid with high economic value. High temperature is the primary limiting factor for its extensive production and cultivation. Breeding heat-resistant varieties is one of the most economical and effective measures to resist heat damage caused by high temperature. In this study, we analyzed the genetic diversity of 24 A. roburghii germplasm resources by Start codon targeted polymorphism (SCoT) molecular markers, and evaluated the heat tolerance of 20 samples by multivariate statistical analysis. The results of genetic diversity analysis showed that at the genetic coefficient was 0.602, A. roburghii germplasm could be divided into two groups: I and II, Group I was divided into two subgroups A and B, Anoectochilus formosanus was classifiedd into Group II alone. In subgroup A, except W from Yunnan, all the other resources were collected in the north and west of Fujian. Subgroup B mainly came from Southern Fujian and Guangxi. The cluster diagram showed that the relatives of A. roburghii from the same origin was close, and the presence or absence of reticulated veins was not the basis for the classification. Five physiological parameters were measured in the 20 samples. It was found that the chlorophyll content and SOD activity decreased obviously after heat treatment, and the relative conductance increased obviously, while the malondialdehyde and soluble protein contents of each resource were different after heat treatment. The preliminary evaluation of heat resistance was conducted by the multivariate statistical analysis method, and the results showed that four resources (TL, L1, A20, GZ1) were heat resistant, while five resources (TJ, HX, L, M, A21) were not.

, correspAuthors=Yimin DAI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Heping LI, Shuhe ZHANG, Jiangbo LIN, Hui ZOU, Yimin DAI), CN=ArticleExt(id=1236324893386657944, articleId=1236324892514242677, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=金线莲资源遗传多样性分析及耐热性评价, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

金线莲(Anoectochilus roburghii)是具有极高经济价值的珍稀濒危兰科药用植物,高温是制约其广泛生产栽培的首要限制因子,选育耐热品种是抵御高温热害最经济有效的措施之一。本研究利用目标起始密码子多态性(SCoT)分子标记技术对24份金线莲种质资源进行遗传多样性分析,并利用多元统计分析的方法对其中20份样本进行耐热性评价。遗传多样性分析结果显示:在遗传系数0.602水平上,可以把金线莲种质分成I和II两大类,I类分成A和B两个亚群,台湾金线莲(TJ)与其他资源遗传距离较远,单独归为II类;A亚群中除了W来源于云南,其他均为福建西北部收集的资源;B亚群主要来自闽南地区和广西。通过对20份金线莲资源5个生理参数测定,发现热处理后叶绿素含量和SOD活性明显下降,相对电导率明显升高,而丙二醛和可溶性蛋白含量热处理后各资源表现不一。利用多元统计分析方法进行耐热性初步评价,结果显示,TL、L1、A20、GZ1等4份资源较为耐热,TJ、HX、L、M、A21等5份资源不耐热。

, correspAuthors=戴艺民, authorNote=null, correspAuthorsNote=
* 戴艺民(DAI Yimin),E-mail:
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李和平(1982—),男,硕士,助理研究员,研究方向:经济作物育种与分子生物学。

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李和平(1982—),男,硕士,助理研究员,研究方向:经济作物育种与分子生物学。

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李和平(1982—),男,硕士,助理研究员,研究方向:经济作物育种与分子生物学。

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金线莲资源遗传多样性分析及耐热性评价
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李和平 , 张树河 , 林江波 , 邹晖 , 戴艺民 *
热带作物学报 | 组学与生物技术 2023,44(12): 2498-2505
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热带作物学报 | 组学与生物技术 2023, 44(12): 2498-2505
金线莲资源遗传多样性分析及耐热性评价
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李和平, 张树河, 林江波, 邹晖, 戴艺民*
作者信息
  • 福建省农业科学院亚热带农业研究所,福建漳州 363005
  • 李和平(1982—),男,硕士,助理研究员,研究方向:经济作物育种与分子生物学。

通讯作者:

* 戴艺民(DAI Yimin),E-mail:
Analysis of Genetic Diversity and Evaluation of Heat Resistance of Anoectochilus roburghii
Heping LI, Shuhe ZHANG, Jiangbo LIN, Hui ZOU, Yimin DAI*
Affiliations
  • Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China
出版时间: 2023-12-25 doi: 10.3969/j.issn.1000-2561.2023.12.015
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金线莲(Anoectochilus roburghii)是具有极高经济价值的珍稀濒危兰科药用植物,高温是制约其广泛生产栽培的首要限制因子,选育耐热品种是抵御高温热害最经济有效的措施之一。本研究利用目标起始密码子多态性(SCoT)分子标记技术对24份金线莲种质资源进行遗传多样性分析,并利用多元统计分析的方法对其中20份样本进行耐热性评价。遗传多样性分析结果显示:在遗传系数0.602水平上,可以把金线莲种质分成I和II两大类,I类分成A和B两个亚群,台湾金线莲(TJ)与其他资源遗传距离较远,单独归为II类;A亚群中除了W来源于云南,其他均为福建西北部收集的资源;B亚群主要来自闽南地区和广西。通过对20份金线莲资源5个生理参数测定,发现热处理后叶绿素含量和SOD活性明显下降,相对电导率明显升高,而丙二醛和可溶性蛋白含量热处理后各资源表现不一。利用多元统计分析方法进行耐热性初步评价,结果显示,TL、L1、A20、GZ1等4份资源较为耐热,TJ、HX、L、M、A21等5份资源不耐热。

金线莲  /  SCoT  /  遗传多样性  /  耐热性

Anoectochilus roburghii is a rare and endangered medicinal orchid with high economic value. High temperature is the primary limiting factor for its extensive production and cultivation. Breeding heat-resistant varieties is one of the most economical and effective measures to resist heat damage caused by high temperature. In this study, we analyzed the genetic diversity of 24 A. roburghii germplasm resources by Start codon targeted polymorphism (SCoT) molecular markers, and evaluated the heat tolerance of 20 samples by multivariate statistical analysis. The results of genetic diversity analysis showed that at the genetic coefficient was 0.602, A. roburghii germplasm could be divided into two groups: I and II, Group I was divided into two subgroups A and B, Anoectochilus formosanus was classifiedd into Group II alone. In subgroup A, except W from Yunnan, all the other resources were collected in the north and west of Fujian. Subgroup B mainly came from Southern Fujian and Guangxi. The cluster diagram showed that the relatives of A. roburghii from the same origin was close, and the presence or absence of reticulated veins was not the basis for the classification. Five physiological parameters were measured in the 20 samples. It was found that the chlorophyll content and SOD activity decreased obviously after heat treatment, and the relative conductance increased obviously, while the malondialdehyde and soluble protein contents of each resource were different after heat treatment. The preliminary evaluation of heat resistance was conducted by the multivariate statistical analysis method, and the results showed that four resources (TL, L1, A20, GZ1) were heat resistant, while five resources (TJ, HX, L, M, A21) were not.

Anoectochilus roburghii (Wall.) Lindl  /  Start codon targeted polymorphism (SCoT)  /  genetic diversity  /  heatresistance
李和平, 张树河, 林江波, 邹晖, 戴艺民. 金线莲资源遗传多样性分析及耐热性评价. 热带作物学报, 2023 , 44 (12) : 2498 -2505 . DOI: 10.3969/j.issn.1000-2561.2023.12.015
Heping LI, Shuhe ZHANG, Jiangbo LIN, Hui ZOU, Yimin DAI. Analysis of Genetic Diversity and Evaluation of Heat Resistance of Anoectochilus roburghii[J]. Chinese Journal of Tropical Crops, 2023 , 44 (12) : 2498 -2505 . DOI: 10.3969/j.issn.1000-2561.2023.12.015
  • 福建省省属公益类科研院所基本科研专项(2020R1030003; 2022R1030003)
  • 福建省农业科学院南药植物科技创新团队项目(CXTD2021001-2)
2023年第44卷第12期
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doi: 10.3969/j.issn.1000-2561.2023.12.015
  • 接收时间:2022-08-22
  • 首发时间:2026-03-05
  • 出版时间:2023-12-25
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出版历史
  • 收稿日期:2022-08-22
  • 修回日期:2022-10-18
基金
福建省省属公益类科研院所基本科研专项(2020R1030003; 2022R1030003)
福建省农业科学院南药植物科技创新团队项目(CXTD2021001-2)
作者信息
    福建省农业科学院亚热带农业研究所,福建漳州 363005

通讯作者:

* 戴艺民(DAI Yimin),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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