Article(id=1236320608234042219, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320602324267792, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.07.005, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1663257600000, receivedDateStr=2022-09-16, revisedDate=1668700800000, revisedDateStr=2022-11-18, acceptedDate=null, acceptedDateStr=null, onlineDate=1772691402728, onlineDateStr=2026-03-05, pubDate=1690214400000, pubDateStr=2023-07-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772691402728, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772691402728, creator=13701087609, updateTime=1772691402728, updator=13701087609, issue=Issue{id=1236320602324267792, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='7', pageStart='1307', pageEnd='1524', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772691401319, creator=13701087609, updateTime=1772694620634, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236334105156187098, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320602324267792, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236334105156187099, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320602324267792, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1348, endPage=1364, ext={EN=ArticleExt(id=1236320609899180981, articleId=1236320608234042219, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Identification and Functional characterization of Protein in Hainan Noni (Morinda citrifolia L.) Fruit at Different Ripening Stages, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Noni fruit is a characteristic fruit of Hainan with a variety of medicinal effects, but there are few studies on its plant proteins. In the present study, two-dimensional gel electrophoresis (2-DE gel) technology based on the BPP+phenol extraction method was used to extract and separate the proteins of noni fruits at different ripening stages including bud stage, half ripe stage, and mature stage by optimizing the experimental conditions to obtain the fingerprint of plant proteins. The gel images were analyzed by Image Master 5.0 software and some proteins with high expression were screened out. The enzymatic hydrolysates of the proteins were further identified by using MALDI-TOF-MS technology. The functional analysis of the highly expressed proteins of three kinds of noni fruits were preliminarily carried out by bioinformatics methods. The results indicated that there were respectively forty four, forty nine, and forty highly expressed proteins at three maturites noni fruits. Among the proteins, there were respectively thirty three, twenty two and thirty nine proteins by mass spectrum identification, which subordinated to various types of protein with different plant functions. Moreover, significant difference existed in the highly expressed proteins of different maturity stages, which involved in a variety of proteins such as chloroplastic ligase, cysteine proteinase, protein kinase, ubiquitin ligase, and auxin response factors. It could provided a scientific and reasonable theoretical guidance for the further development and utilization of the plant protein of noni fruits.

, correspAuthors=Zhendong ZHAO, Wenying HE, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Jing QIU, Yuan SONG, Ni WANG, Jiaren WANG, Jing CHEN, Guangying CHEN, Zhendong ZHAO, Wenying HE), CN=ArticleExt(id=1236320610176005062, articleId=1236320608234042219, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=海南不同成熟期诺丽果的蛋白鉴定及功能分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

诺丽果（noni）是海南的一种特色水果，具有多种药效价值，但关于其植物蛋白的研究却很少。本研究采用基于BPP+酚提取法的双向电泳技术，通过优化实验条件，对花蕾期、半成熟及成熟期等3个时期产自海南的诺丽果实进行蛋白质的提取分离，获得其植物蛋白指纹图谱；利用Image Master 5.0软件分析凝胶图像筛选出高表达蛋白；再利用MALDI-TOF-MS技术对这些蛋白酶解产物进行质谱鉴定；通过生物信息学手段对3种诺丽果实的高表达蛋白初步进行蛋白功能分析。结果表明：花蕾期、半成熟及成熟诺丽果实分别有44、49和40个高表达蛋白，通过质谱鉴定，确定了其中分别有33、22和39个蛋白，从属不同植物功能的蛋白种类；不同成熟期的高表达蛋白差异较大，涉及叶绿体连接酶、半胱氨酸蛋白酶、蛋白激酶、泛素连接酶、生长素反应因子等多种蛋白质。该研究为进一步开发利用诺丽果的植物蛋白提供科学合理的理论指导。

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邱静（2000—），女，本科生，研究方向：植物蛋白质组学。

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邱静（2000—），女，本科生，研究方向：植物蛋白质组学。

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