热带作物学报

木霉与椰子水复配中量元素水溶肥促进黄瓜种子萌发和幼苗生长

PDF下载

徐宁 , 徐秋实 , 王剑宁 , 施雨桐 , 刘震 , 刘铜 ^*

热带作物学报 | 作物栽培与生理生化 2023,44(7): 1426-1434

收起

热带作物学报 | 作物栽培与生理生化 2023, 44(7): 1426-1434

木霉与椰子水复配中量元素水溶肥促进黄瓜种子萌发和幼苗生长

全屏

徐宁, 徐秋实, 王剑宁, 施雨桐, 刘震, 刘铜^*

作者信息

海南大学植物保护学院/海南省绿色农用生物制剂创制工程中心，海南海口　570228

徐宁（1998—），男，硕士研究生，研究方向：植物病理学。

通讯作者:

^* 刘铜（LIU Tong），E-mail: liutongamy@sina.com。

Water-soluble Fertilizer Compounded with Medium Element in Trichoderma spp. and Coconut Water Promoted Cucumber Seed Germination and Seedling Growth

Ning XU, Qiushi XU, Jianning WANG, Yutong SHI, Zhen LIU, Tong LIU^*

Affiliations

School of Plant Protection, Hainan University/Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan, Haikou, Hainan 570228, China

出版时间: 2023-07-25 doi: 10.3969/j.issn.1000-2561.2023.07.013

文章导航

摘要

收起

木霉菌在绿色农业病害防控中是一种重要的生防功能微生物，普遍开发为木霉菌剂用于生物防治、生物肥料及土壤改良剂。传统木霉菌剂的开发局限于木霉菌株的筛选及发酵条件的优化等，因此探索木霉菌新型应用方式具有重要的意义。椰子水在椰子工业生产中因其不耐储存、运输及开发成本高常作为废弃物直接排放丢弃。本研究以木霉孢子粉和热带废弃椰子水为材料，将废弃椰子水与木霉孢子粉复配成中量元素水溶肥，探究该水溶肥对黄瓜种子萌发与黄瓜幼苗生理指标的影响。结果表明：椰子水中添加中量元素并调整pH 7对木霉产孢效果最好，产孢量达到2.32×10⁷ CFU/mL；木霉与椰子水复配中量元素水溶肥（A处理）和椰子水与木霉复配水溶肥（B处理）黄瓜种子萌发率分别为94.44%、87.50%，相对于无菌水（CK）提高了28.30%、18.87%，显著促进黄瓜种子的萌发。盆栽试验表明A处理株高与根长分别为24.01、27.76 cm，较CK分别提高48.03%、47.27%，显著促进黄瓜幼苗的生长；同时该处理组黄瓜幼苗茎鲜重与茎干重以及根鲜重与根干重也显著高于CK。进一步测定黄瓜幼苗的总叶绿素、还原糖与可溶性蛋白含量以及过氧化物酶（POD）与过氧化氢酶（CAT）活性，相较于CK分别提高97.46%、66.32%、58.85%、32.67%、40.31%；并且能显著降低丙二醛（MDA）含量，较CK降低79.96%。综上，木霉与椰子水复配中量元素水溶肥处理组可以显著促进黄瓜种子萌发和幼苗生长，提高了幼苗的总叶绿素、还原糖、可溶性蛋白含量以及POD和CAT活性，降低MDA含量，增强黄瓜抵抗逆境胁迫的能力。

关键词

木霉 / 椰子水 / 水溶肥 / 黄瓜

Abstract

收起

Trichoderma spp. is an important biocontrol microorganism in the fungal disease prevention and control of green agriculture. Which is widely developed as Trichoderma agent for biological control, biofertilizer and soil amendment. The development of traditional Trichoderma fungicide is limited to the screening of Trichoderma strains and the optimization of fermentation conditions. Therefore, it is necessary to explore a new application mode of Trichoderma. Due to intolerance to storage and high costs of transportation and development, coconut water is often directly discarded as waste in the production of coconut industry. In this study, coconut water was compounded with Trichoderma spores and medium elements as water-soluble fertilizers to explore the effects on seed germination and seedling physiological indexes of cucumber. The results showed that adding medium element to coconut water and adjusting pH to 7 had the best effect on the production of Trichoderma spore, and the sporulation reached 2.32×10⁷ CFU/mL. The treatment group A of water-soluble fertilizer compounded with medium element in Trichoderma spp. and coconut water and treatment group B of water-soluble fertilizer compounded with Trichoderma spp. and coconut water significantly promoted the germination of cucumber seeds, and the germination rates of cucumber seeds was 94.44%and 87.50%, respectively, compared with sterile water (CK), which increased by 28.30% and 18.87%, respectively. Pot experiment showed that the plant height and root length of treatment group A was 24.01 cm and 27.76 cm, respectively, which was 48.03% and 47.27% higher than those of CK, which significantly promoted the growth of cucumber seedlings. At the same time, the fresh weight and dry weight of stem and root of cucumber seedlings in treatment group A were also significantly higher than those of CK. Further, we determined the content of total chlorophyll, reducing sugar and soluble protein, the activity of peroxidase (POD) and catalase (CAT) of cucumber seedlings in treatment group A, compared with CK, which increased by 97.46%, 66.32%, 58.85%, 32.67%, and 40.31%, respectively; and the content of malondialdehyde (MDA) was significantly decreased, which was 79.96% lower than that of CK. In summary, the treatment group of water-soluble fertilizer compounded with medium element in Trichoderma spp. and coconut water could significantly promote cucumber seed germination and seedling growth, improve the contents of total chlorophyll, reducing sugar, soluble protein, POD and CAT activity, decrease MDA content of seedlings, and enhance the ability of cucumber to resist stress.

Key words

Trichoderma / coconut water / water-soluble fertilizer / cucumber

引用本文

徐宁, 徐秋实, 王剑宁, 施雨桐, 刘震, 刘铜. 木霉与椰子水复配中量元素水溶肥促进黄瓜种子萌发和幼苗生长. 热带作物学报, 2023 , 44 (7) : 1426 -1434 . DOI: 10.3969/j.issn.1000-2561.2023.07.013

Ning XU, Qiushi XU, Jianning WANG, Yutong SHI, Zhen LIU, Tong LIU. Water-soluble Fertilizer Compounded with Medium Element in Trichoderma spp. and Coconut Water Promoted Cucumber Seed Germination and Seedling Growth[J]. Chinese Journal of Tropical Crops, 2023 , 44 (7) : 1426 -1434 . DOI: 10.3969/j.issn.1000-2561.2023.07.013

基金

收起

国家级大学生创新创业训练项目(202110589047)

参考文献

收起

2023年第44卷第7期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.3969/j.issn.1000-2561.2023.07.013

接收时间：2022-06-21
首发时间：2026-03-05
出版时间：2023-07-25

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2022-06-21
修回日期：2022-09-06

基金

国家级大学生创新创业训练项目(202110589047)

作者信息

海南大学植物保护学院/海南省绿色农用生物制剂创制工程中心，海南海口　570228

通讯作者:

^* 刘铜（LIU Tong），E-mail: liutongamy@sina.com。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.07.013

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT