Article(id=1236320194667270880, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.08.020, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1659283200000, receivedDateStr=2022-08-01, revisedDate=1663171200000, revisedDateStr=2022-09-15, acceptedDate=null, acceptedDateStr=null, onlineDate=1772691304127, onlineDateStr=2026-03-05, pubDate=1692892800000, pubDateStr=2023-08-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772691304127, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772691304127, creator=13701087609, updateTime=1772691304127, updator=13701087609, issue=Issue{id=1236320187016860250, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='8', pageStart='1525', pageEnd='1733', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=0, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772691302302, creator=13701087609, updateTime=1772691825718, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236322382437216680, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236322382437216681, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1698, endPage=1703, ext={EN=ArticleExt(id=1236320195485160184, articleId=1236320194667270880, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver, columnId=1236292524264968282, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Protection & Bio-safety, runingTitle=null, highlight=null, articleAbstract=

In order to clearify the potential of entomopathogenic nemotodes (EPNs) on the control of Rhynchophorus ferrugineus, the pathogenucity of Heterorhabditis bacteriophora H06 against all stages of R. ferrugineus and the behavior of the invading red palm weevil larvae was studied using laboratory bioassay methods. The results showed that the mortality of eggs was the highest in all stages after R. ferrugineus was infected by EPNs, and LC50 was 104.85 IJs/mL, followed by 1-2 instar larvae, 3-4 instar larvae and 5-6 instar larvae, LC50 was 165.34 IJs/mL, 215.31 IJs/mL and 292.26 IJs/mL respectively; No significant differences were observed in the sensitivity between 7-10 instar larvae and pupae to EPNs. Among the effects of inoculation site on the mortality of different instar larvae, the mortality of the 3rd instar larvae was the highest when inoculated with spiracle and anus, which was 91.11% and 78.89% respectively, while the lethal rate of inoculation with mouthparts was the highest in the treatment of the 6th and 8th instar larvae. No significant difference presented in the mortality of different instar R. ferrugineus larvae when treated with vent inoculation. The R. ferrugineus body color changed to red or reddish brown, no spontaneous response when touching, and the body wall of R. ferrugineus shrinked after treated by EPNs. When the nematode approached the spiracle of R. ferrugineus larval, it stayed around the spiracle for a moment, and finally entered the insect body, and the number of EPNs successfully invaded was about 33.33%.

, correspAuthors=Baozhu ZHONG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG, Chuyu ZHANG, Tuo CHEN, Wenlian LI), CN=ArticleExt(id=1236320196202386200, articleId=1236320194667270880, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及侵染行为, columnId=1236292524520820846, journalTitle=热带作物学报, columnName=植物保护与生物安全, runingTitle=null, highlight=null, articleAbstract=

为阐明昆虫病原线虫(entomopathogenic nematodes, EPNs)对红棕象甲(Rhynchophorus ferrugineus)的防控潜力,采用室内生物测定法研究了嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及其侵染行为学特性。结果表明,各虫态红棕象甲被EPNs侵染后,卵的死亡率最高,LC50为104.85 IJs/mL,其次为1~2龄幼虫、3~4龄幼虫和5~6龄幼虫,其LC50分别为165.34、215.31、292.26 IJs/mL;7~10龄幼虫和蛹对EPNs的敏感性无显著差异;不同接种部位对不同龄期幼虫死亡率的影响中,3龄幼虫处理以气孔和肛门接种的死亡率较高,分别为91.11%和78.89%,而6龄和8龄幼虫处理中均以口器接种致死率最高;在肛门接种处理中,各龄期的红棕象甲幼虫死亡率无显著差异。经EPNs侵染后,红棕象甲各虫态体色均变成红色或红褐色,轻触虫体无自主反应,同时幼虫伴随体壁皱缩;线虫接近供试虫体表气孔后,会在气孔周围停留片刻,最后从气孔处进入虫体,成功侵入的EPNs个体数量约占33.33%。

, correspAuthors=钟宝珠, authorNote=null, correspAuthorsNote=
* 钟宝珠(ZHONG Baozhu),E-mail:
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吕朝军(1980—),男,博士,副研究员,研究方向:热带有害生物预警监测与综合治理。

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吕朝军(1980—),男,博士,副研究员,研究方向:热带有害生物预警监测与综合治理。

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吕朝军(1980—),男,博士,副研究员,研究方向:热带有害生物预警监测与综合治理。

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嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及侵染行为
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吕朝军 , 赵建超 , 钟宝珠 * , 张楚毓 , 陈拓 , 李文莲
热带作物学报 | 植物保护与生物安全 2023,44(8): 1698-1703
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热带作物学报 | 植物保护与生物安全 2023, 44(8): 1698-1703
嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及侵染行为
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吕朝军, 赵建超, 钟宝珠* , 张楚毓, 陈拓, 李文莲
作者信息
  • 中国热带农业科学院椰子研究所,海南文昌 571339
  • 吕朝军(1980—),男,博士,副研究员,研究方向:热带有害生物预警监测与综合治理。

通讯作者:

* 钟宝珠(ZHONG Baozhu),E-mail:
Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver
Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG* , Chuyu ZHANG, Tuo CHEN, Wenlian LI
Affiliations
  • Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China
出版时间: 2023-08-25 doi: 10.3969/j.issn.1000-2561.2023.08.020
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为阐明昆虫病原线虫(entomopathogenic nematodes, EPNs)对红棕象甲(Rhynchophorus ferrugineus)的防控潜力,采用室内生物测定法研究了嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及其侵染行为学特性。结果表明,各虫态红棕象甲被EPNs侵染后,卵的死亡率最高,LC50为104.85 IJs/mL,其次为1~2龄幼虫、3~4龄幼虫和5~6龄幼虫,其LC50分别为165.34、215.31、292.26 IJs/mL;7~10龄幼虫和蛹对EPNs的敏感性无显著差异;不同接种部位对不同龄期幼虫死亡率的影响中,3龄幼虫处理以气孔和肛门接种的死亡率较高,分别为91.11%和78.89%,而6龄和8龄幼虫处理中均以口器接种致死率最高;在肛门接种处理中,各龄期的红棕象甲幼虫死亡率无显著差异。经EPNs侵染后,红棕象甲各虫态体色均变成红色或红褐色,轻触虫体无自主反应,同时幼虫伴随体壁皱缩;线虫接近供试虫体表气孔后,会在气孔周围停留片刻,最后从气孔处进入虫体,成功侵入的EPNs个体数量约占33.33%。

嗜菌异小杆线虫  /  致病力  /  红棕象甲  /  形态学

In order to clearify the potential of entomopathogenic nemotodes (EPNs) on the control of Rhynchophorus ferrugineus, the pathogenucity of Heterorhabditis bacteriophora H06 against all stages of R. ferrugineus and the behavior of the invading red palm weevil larvae was studied using laboratory bioassay methods. The results showed that the mortality of eggs was the highest in all stages after R. ferrugineus was infected by EPNs, and LC50 was 104.85 IJs/mL, followed by 1-2 instar larvae, 3-4 instar larvae and 5-6 instar larvae, LC50 was 165.34 IJs/mL, 215.31 IJs/mL and 292.26 IJs/mL respectively; No significant differences were observed in the sensitivity between 7-10 instar larvae and pupae to EPNs. Among the effects of inoculation site on the mortality of different instar larvae, the mortality of the 3rd instar larvae was the highest when inoculated with spiracle and anus, which was 91.11% and 78.89% respectively, while the lethal rate of inoculation with mouthparts was the highest in the treatment of the 6th and 8th instar larvae. No significant difference presented in the mortality of different instar R. ferrugineus larvae when treated with vent inoculation. The R. ferrugineus body color changed to red or reddish brown, no spontaneous response when touching, and the body wall of R. ferrugineus shrinked after treated by EPNs. When the nematode approached the spiracle of R. ferrugineus larval, it stayed around the spiracle for a moment, and finally entered the insect body, and the number of EPNs successfully invaded was about 33.33%.

Heterorhabditis bacteriophora  /  pathogenicity  /  Rhynchophorus ferrugineus Oliver  /  morphological
吕朝军, 赵建超, 钟宝珠, 张楚毓, 陈拓, 李文莲. 嗜菌异小杆线虫H06品系对红棕象甲各虫态的致病力及侵染行为. 热带作物学报, 2023 , 44 (8) : 1698 -1703 . DOI: 10.3969/j.issn.1000-2561.2023.08.020
Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG, Chuyu ZHANG, Tuo CHEN, Wenlian LI. Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver[J]. Chinese Journal of Tropical Crops, 2023 , 44 (8) : 1698 -1703 . DOI: 10.3969/j.issn.1000-2561.2023.08.020
  • 海南省自然科学基金高层次人才项目(320RC740)
2023年第44卷第8期
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doi: 10.3969/j.issn.1000-2561.2023.08.020
  • 接收时间:2022-08-01
  • 首发时间:2026-03-05
  • 出版时间:2023-08-25
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  • 收稿日期:2022-08-01
  • 修回日期:2022-09-15
基金
海南省自然科学基金高层次人才项目(320RC740)
作者信息
    中国热带农业科学院椰子研究所,海南文昌 571339

通讯作者:

* 钟宝珠(ZHONG Baozhu),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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