Article(id=1236320187838951701, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.08.008, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1661702400000, receivedDateStr=2022-08-29, revisedDate=1664467200000, revisedDateStr=2022-09-30, acceptedDate=null, acceptedDateStr=null, onlineDate=1772691302499, onlineDateStr=2026-03-05, pubDate=1692892800000, pubDateStr=2023-08-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772691302499, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772691302499, creator=13701087609, updateTime=1772691302499, updator=13701087609, issue=Issue{id=1236320187016860250, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='8', pageStart='1525', pageEnd='1733', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=0, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772691302302, creator=13701087609, updateTime=1772691825718, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236322382437216680, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236322382437216681, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1588, endPage=1595, ext={EN=ArticleExt(id=1236320188115775767, articleId=1236320187838951701, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Establishment of Regeneration System of Stem Callus for Ardisia gigantifolia, a Rare and Endangered Species, columnId=1236256430060261740, journalTitle=Chinese Journal of Tropical Crops, columnName=Germplasm Resources, Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

Ardisia gigantifolia Stapf is native to Hekou county, Yunnan province, with few wild individuals, and is listed as a critically endangered species. It has important protection value and good prospect of garden development and application. The stem segments of aseptic seedlings of A. gigantifolia were used as the explants to establish a propagation in vitro. The results showed that the optimal medium for callus induction was WPM+0.50 mg/L TDZ+0.10 mg/L NAA, and the induction rate of callus was 96.67%. The optional medium for callus proliferation was WPM+0.50 mg/L TDZ and the multiplication coefficient was 4.42. The optimal medium for callus differentiation was WPM+2.00 mg/L 6-BA+0.20 mg/L NAA, and the induction rate reached 100%. The optional medium for adventitious bud proliferation culture was WPM+4.00 mg/L 6-BA, the multiplication coefficient was 3.90, and the cluster buds grew well. The optional rooting medium was WPM+10% coconut water+0.20 mg/L NAA, the rooting rate reached 100%, the root system developed well and the test-tube plantlets grew vigorously. Plantlets were transplanted into the mixture substrate with the volume ratio of perlite and peat soil of 1∶3, the survival rate was 96.67% after 60 days and plants grew well. This study can provide technical support for the large-scale seedling production of A. gigantifolia, as well as theoretical basis and technical support for the protection, seedling breeding, and molecular biology research of this species.

, correspAuthors=Zulin NING, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yan ZHOU, Dongmei LI, Kunlin WU, Zulin NING), CN=ArticleExt(id=1236320188350656795, articleId=1236320187838951701, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=濒危植物粗茎紫金牛茎段愈伤组织再生体系的建立, columnId=1236256430219645304, journalTitle=热带作物学报, columnName=种质资源与遗传育种, runingTitle=null, highlight=null, articleAbstract=

粗茎紫金牛（Ardisia gigantifolia Stapf）原产于云南河口县，野生个体数量稀少，被列为极危物种，其叶、花、果均具较好的观赏性，具有重要的保护价值和较好的园林开发应用前景。本研究以粗茎紫金牛茎段为外植体建立其高效愈伤组织再生体系。结果表明：最佳愈伤组织诱导培养基为WPM+0.50 mg/L TDZ+0.10 mg/L NAA，诱导率为96.67%；愈伤组织在培养基WPM+0.50 mg/L TDZ中增殖系数达4.42，在培养基WPM+2.00 mg/L 6-BA+0.20 mg/L NAA中不定芽诱导率为100%；不定芽在培养基WPM+4.00 mg/L 6-BA中增殖系数达3.90，于培养基WPM+10% CW+0.20 mg/L NAA中生根率为100%，且不定根发达，植株生长健壮；组培苗移栽至珍珠岩∶泥炭土=1∶3的混合基质中，60 d后成活率达96.67%。该研究可为种苗的规模化生产提供技术支撑，也为该物种的保护、种苗繁育和分子生物学研究提供理论基础和技术支持。

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周艳（1994—），女，硕士，研究实习员，研究方向：园林植物种质资源及利用。

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周艳（1994—），女，硕士，研究实习员，研究方向：园林植物种质资源及利用。

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