Article(id=1236320187490816603, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.08.006, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1662566400000, receivedDateStr=2022-09-08, revisedDate=1665244800000, revisedDateStr=2022-10-09, acceptedDate=null, acceptedDateStr=null, onlineDate=1772691302415, onlineDateStr=2026-03-05, pubDate=1692892800000, pubDateStr=2023-08-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772691302415, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772691302415, creator=13701087609, updateTime=1772691302415, updator=13701087609, issue=Issue{id=1236320187016860250, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='8', pageStart='1525', pageEnd='1733', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=0, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772691302302, creator=13701087609, updateTime=1772691825718, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236322382437216680, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236322382437216681, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236320187016860250, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1569, endPage=1578, ext={EN=ArticleExt(id=1236320187746669149, articleId=1236320187490816603, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Cloning and Expression Analysis of PR-10 Gene from Solanum sisymbriifolium Lam., columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Verticillium wilt, a soil-borne disease caused by Verticillium spp., is one of the most important diseases in eggplant production. It seriously affects the yield and quality of eggplant. At present, the control methods for eggplant verticillium wilt are limited to grafting and chemical controls, but neither of them can prevent the occurrence of verticillium wilt. The best way to control Verticillium wilt is to explore the verticillium wilt resistance genes in eggplant and to breed some resistant eggplant varieties with genetic engineering technology. Solanum symbriifolium Lam. is a wild eggplant in Yunnan, China with a high Verticillium wilt resistance. PR genes encode pathogenesis-related proteins, which are closely related to plant disease defense. Among them, PR10 protein is a nuclease-like protein, and the defense mechanism of PR10 protein in S. sisymbriifolium is still unclear. In this study, a wild eggplant S. sisymbriifolium was used as the experimental material. The pathogenesis related protein 10 (PR10) homologous gene was isolated and cloned by reverse transcription PCR (RT-PCR) technology from the previously established transcriptome database of S. sisymbriifolium and named as SsPR-10. The cloned sequence length of SsPR-10 was 645 bp, and its coding region was 480 bp long, encoding a protein of 159 amino acids residues in total. And the bioinformatics analysis showed that SsPR-10 protein was an acidic hydrophilic protein with a molecular mass of 17.71 kDa and a theoretical isoelectric point (pI) of 5.54. The prediction of transmembrane region and signal peptide showed that SsPR-10 protein had no transmembrane region and signal peptide. The prediction for subcellular localization showed that SsPR-10 was localized in the cytoplasm of plant cell. The results of conserved domain prediction showed that SsPR-10 contained a "P-LOOP" circle structure (sequence positions at 47-52 bp) and conserved sequence PATHOGENESIS_BETVI (sequence positions at 88-120 bp). The sequence alignment and phylogenetic tree analysis showed that SsPR-10 protein had the highest homology with PR10 protein from S. virginianum, followed by STH-2 protein from S. tuberosum. The expression of SsPR-10 gene was analyzed by real-time fluorescence quantitative PCR (RT-qPCR) experiments, and the expression level of SsPR-10 was the highest in the root than in other organs of S. sisymbriifolium, showing a tissue specificity. The expression level of SsPR-10 was multiplied to 8.16 times at 24 h after inoculation with V. dahliae, indicating that verticillium wilt might induce the expression of SsPR-10. In this study, SsPR-10 gene was cloned and its expression pattern was analyzed in the wild eggplant S. sisymbriifolium for the first time, which will provide a theoretical basis for further studying the mechanism of SsPR-10 responding to Verticillium wilt and other biological stresses.

, correspAuthors=Liyan WU, Guanghui DU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Jie CHENG, Hanxue ZHANG, Yanan YU, Mengying YIN, Xiangshu DONG, Liyan WU, Guanghui DU), CN=ArticleExt(id=1236320188052853343, articleId=1236320187490816603, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=蒜芥茄PR-10基因的克隆与表达分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

黄萎病是由轮枝菌属（Verticillium spp.）真菌引起的一种土传病害，是茄子生产中的重要病害之一，严重影响茄子的产量和品质。目前，对于茄子黄萎病的防治还限于嫁接和化学防治，但二者均不能较好防止该病的发生，而挖掘茄子中黄萎病抗性基因，结合基因工程技术培育抗病品种，是防治黄萎病的最佳途径。蒜芥茄（Solanum sisymbriifolium Lam.）为云南野生茄，对黄萎病具有高抗性。PR基因是编码植物病程相关蛋白（pathogenesis related protein）的基因，与植物抗病防御密切相关，其中，PR10蛋白是具有核酸酶相似结构的一类蛋白，但其在蒜芥茄中的抗病作用机制尚不明确。本研究以蒜芥茄为试验材料，利用前期建立的蒜芥茄转录组数据库，通过RT-PCR技术从中分离并克隆得到PR10同源基因，命名为SsPR-10，测序得到645 bp，其中基因编码区长480 bp，共编码159个氨基酸。将测序得到的编码区序列进行生物信息学分析，结果显示：SsPR-10蛋白是分子量为17.71 kDa、等电点为5.54的酸性亲水蛋白；跨膜区和信号肽预测显示SsPR-10蛋白无跨膜区和信号肽；亚细胞定位结果表明SsPR-10定位于细胞质；保守结构域预测结果显示，SsPR-10含有“P-LOOP”环（47~52位）和保守序列PATHOGENESIS_BETVI（88~120位）。序列比对与系统进化树分析结果表明，SsPR-10蛋白与黄果茄PR10蛋白同源性最高，其次是马铃薯STH-2蛋白。通过实时荧光定量PCR（RT-qPCR）技术对SsPR-10基因的表达进行分析，SsPR-10在蒜芥茄根部的表达量最高，具有组织特异性；在接种大丽轮枝菌（V. dahliae）24 h后，SsPR-10的表达量达到初始表达量的8.16倍，表明黄萎病病菌可能诱导SsPR-10的表达。本研究对野生蒜芥茄PR-10基因进行了克隆和表达分析，为进一步研究SsPR-10基因响应黄萎病等生物胁迫的机制提供一定的理论基础。

, correspAuthors=吴丽艳, 杜光辉, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=6dlkoQRJLPyL81sTrq4+LQ==, magXml=JkHbAvmckIRq88jW77vHAQ==, pdfUrl=null, pdf=4jptVwbBls1iw5iRmAiJJQ==, pdfFileSize=18390684, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=13IaBTcDUsCq0ccvY0JkGg==, mapNumber=null, authorCompany=null, fund=null, authors=, authorsList=程捷, 张涵雪, 蔚亚楠, 尹梦莹, 董相书, 吴丽艳, 杜光辉)}, authors=[Author(id=1236331656597009103, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1236320187490816603, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1236331656714449623, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1236320187490816603, authorId=1236331656597009103, language=EN, stringName=Jie CHENG, firstName=Jie, middleName=null, lastName=CHENG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=^1.Institute of Plant Resources, Yunnan University, Kunming, Yunnan 650500, China
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^2.云南省农业科学院园艺作物研究所，云南昆明　650205, bio={"content":"

程捷（1999—），女，硕士研究生，研究方向：茄果类蔬菜分子育种。

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程捷（1999—），女，硕士研究生，研究方向：茄果类蔬菜分子育种。

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