Article(id=1236318328910836696, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236318325282763627, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.02.010, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1646064000000, receivedDateStr=2022-03-01, revisedDate=1651939200000, revisedDateStr=2022-05-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1772690859295, onlineDateStr=2026-03-05, pubDate=1677254400000, pubDateStr=2023-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772690859295, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772690859295, creator=13701087609, updateTime=1772690859295, updator=13701087609, issue=Issue{id=1236318325282763627, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='2', pageStart='225', pageEnd='445', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772690858431, creator=13701087609, updateTime=1772695119289, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236336196671033969, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236318325282763627, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236336196675228274, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236318325282763627, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=310, endPage=316, ext={EN=ArticleExt(id=1236318330794078248, articleId=1236318328910836696, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli, columnId=1236256434120348225, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Cultivation, Physiology & Biochemistry, runingTitle=null, highlight=null, articleAbstract=

Laticifer cells present in aging calli of Hevea brasiliensis. Methyl jasmonate (MeJA) and jasmonic acid (JA) belong to jasmonates (JAs). However, the effect of MeJA on laticifer cell differentiation of the rubber tree anther calli is unclear. In the present paper, the effect of MeJA on laticifer cell differentiation of anther calli was systematically studied. MeJA added to media in the range of 0-1 mg/L did not influence the growth of anther calli, while higher concentration of MeJA in media in the range of 2-3 mg/L severely inhibited callus growth. MeJA concentration in media was negatively correlated with callus growth. Histochemical sections showed that low concentration of MeJA in media greatly promoted the differentiation of callus laticifer cells and remarkably increased the occurring frequency, while high concentration of MeJA in media gravely impeded callus laticifer cell differentiation and strikingly decreased the occurring frequency. Among them, 1 mg/L MeJA in media was the optimal concentration to improve the differentiation of laticifer cells in calli. qPCR results showed that when the media were supplemented with MeJA, the change trend of the expression level of genes that was essential in latex biosynthesis, such as small rubber particle protein (SRPP), rubber elongation factor (REF), and cis-prenyl transferase (CPT), was basically consistent with that of the occurring frequency of callus laticifer cells, which further confirmed that MeJA promoted the differentiation of callus laticifer cells. Transmission electron microscopy observation showed that callus laticifer cells contained some specific organelles, such as rubber particles and lutoids. The results of this paper are of great significance for promoting the application of rubber tree callus in the study of laticifer differentiation, and for developing rubber tree novel yield-increasing reagents.

, correspAuthors=Deguan TAN, Jiaming ZHANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Zihan GUO, Deguan TAN, Dongyi HUANG, Lili FU, Xuepiao SUN, Jiaming ZHANG), CN=ArticleExt(id=1236318331460972640, articleId=1236318328910836696, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=茉莉酸甲酯对橡胶树花药愈伤乳管细胞分化的影响, columnId=1236256434313286224, journalTitle=热带作物学报, columnName=作物栽培与生理生化, runingTitle=null, highlight=null, articleAbstract=

橡胶树老化的愈伤组织存在乳管细胞。茉莉酸甲酯(MeJA)与茉莉酸(JA)同属茉莉酸类物质(JAs),但MeJA对橡胶树愈伤乳管细胞分化的效果尚不清楚。系统研究MeJA对橡胶树愈伤乳管细胞分化的效果,结果发现,低浓度的MeJA(0~1 mg/L)对花药愈伤组织的生长影响不明显,而高浓度的MeJA(2~3 mg/L)会抑制愈伤组织生长,且MeJA浓度与愈伤组织生长呈负相关关系。组织化学制片结果显示,低浓度的MeJA能显著提高愈伤乳管细胞发生频率,其中浓度为1 mg/L效果最佳;而高浓度的MeJA则会降低其发生频率。荧光定量PCR结果显示,培养基中添加MeJA后SRPPREFCPT等与橡胶生物合成相关的重要基因的表达量在愈伤组织中变化趋势与愈伤乳管细胞发生频率的变化趋势基本一致,进一步证实MeJA能促进愈伤乳管细胞分化。透射电镜观察发现愈伤乳管细胞内含有橡胶粒子、黄色体等细胞器。研究结果对促进橡胶树愈伤组织在乳管分化研究中的应用,以及开发新型橡胶树增产刺激剂,促进橡胶树天然橡胶的生产具有一定意义。

, correspAuthors=谭德冠, 张家明, authorNote=null, correspAuthorsNote=
* 谭德冠(TAN Deguan),E-mail:
张家明(ZHANG Jiaming),E-mail:
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郭子晗(1996—),女,硕士研究生,研究方向:橡胶树生物技术。

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郭子晗(1996—),女,硕士研究生,研究方向:橡胶树生物技术。

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郭子晗(1996—),女,硕士研究生,研究方向:橡胶树生物技术。

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茉莉酸甲酯对橡胶树花药愈伤乳管细胞分化的影响
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郭子晗 1, 2 , 谭德冠 2, * , 黄东益 1 , 付莉莉 2 , 孙雪飘 2 , 张家明 2, *
热带作物学报 | 作物栽培与生理生化 2023,44(2): 310-316
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热带作物学报 | 作物栽培与生理生化 2023, 44(2): 310-316
茉莉酸甲酯对橡胶树花药愈伤乳管细胞分化的影响
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郭子晗1, 2, 谭德冠2, * , 黄东益1, 付莉莉2, 孙雪飘2, 张家明2, *
作者信息
  • 1.海南大学热带作物学院,海南海口 570228
  • 2.中国热带农业科学院热带生物技术研究所/中国热带农业科学院海南热带农业资源研究院/农业农村部热带作物生物学与遗传资源利用重点实验室,海南海口 571101
  • 郭子晗(1996—),女,硕士研究生,研究方向:橡胶树生物技术。

通讯作者:

* 谭德冠(TAN Deguan),E-mail:
张家明(ZHANG Jiaming),E-mail:
Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli
Zihan GUO1, 2, Deguan TAN2, * , Dongyi HUANG1, Lili FU2, Xuepiao SUN2, Jiaming ZHANG2, *
Affiliations
  • 1.College of Tropical Crops, Hainan University, Haikou, Hannan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Hainan Academy of Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
出版时间: 2023-02-25 doi: 10.3969/j.issn.1000-2561.2023.02.010
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橡胶树老化的愈伤组织存在乳管细胞。茉莉酸甲酯(MeJA)与茉莉酸(JA)同属茉莉酸类物质(JAs),但MeJA对橡胶树愈伤乳管细胞分化的效果尚不清楚。系统研究MeJA对橡胶树愈伤乳管细胞分化的效果,结果发现,低浓度的MeJA(0~1 mg/L)对花药愈伤组织的生长影响不明显,而高浓度的MeJA(2~3 mg/L)会抑制愈伤组织生长,且MeJA浓度与愈伤组织生长呈负相关关系。组织化学制片结果显示,低浓度的MeJA能显著提高愈伤乳管细胞发生频率,其中浓度为1 mg/L效果最佳;而高浓度的MeJA则会降低其发生频率。荧光定量PCR结果显示,培养基中添加MeJA后SRPPREFCPT等与橡胶生物合成相关的重要基因的表达量在愈伤组织中变化趋势与愈伤乳管细胞发生频率的变化趋势基本一致,进一步证实MeJA能促进愈伤乳管细胞分化。透射电镜观察发现愈伤乳管细胞内含有橡胶粒子、黄色体等细胞器。研究结果对促进橡胶树愈伤组织在乳管分化研究中的应用,以及开发新型橡胶树增产刺激剂,促进橡胶树天然橡胶的生产具有一定意义。

橡胶树  /  愈伤组织  /  乳管细胞分化  /  茉莉酸甲酯  /  基因表达

Laticifer cells present in aging calli of Hevea brasiliensis. Methyl jasmonate (MeJA) and jasmonic acid (JA) belong to jasmonates (JAs). However, the effect of MeJA on laticifer cell differentiation of the rubber tree anther calli is unclear. In the present paper, the effect of MeJA on laticifer cell differentiation of anther calli was systematically studied. MeJA added to media in the range of 0-1 mg/L did not influence the growth of anther calli, while higher concentration of MeJA in media in the range of 2-3 mg/L severely inhibited callus growth. MeJA concentration in media was negatively correlated with callus growth. Histochemical sections showed that low concentration of MeJA in media greatly promoted the differentiation of callus laticifer cells and remarkably increased the occurring frequency, while high concentration of MeJA in media gravely impeded callus laticifer cell differentiation and strikingly decreased the occurring frequency. Among them, 1 mg/L MeJA in media was the optimal concentration to improve the differentiation of laticifer cells in calli. qPCR results showed that when the media were supplemented with MeJA, the change trend of the expression level of genes that was essential in latex biosynthesis, such as small rubber particle protein (SRPP), rubber elongation factor (REF), and cis-prenyl transferase (CPT), was basically consistent with that of the occurring frequency of callus laticifer cells, which further confirmed that MeJA promoted the differentiation of callus laticifer cells. Transmission electron microscopy observation showed that callus laticifer cells contained some specific organelles, such as rubber particles and lutoids. The results of this paper are of great significance for promoting the application of rubber tree callus in the study of laticifer differentiation, and for developing rubber tree novel yield-increasing reagents.

Hevea brasiliensis Muell. Arg.  /  callus  /  laticifer cell differentiation  /  methyl jasmonate  /  gene expression
郭子晗, 谭德冠, 黄东益, 付莉莉, 孙雪飘, 张家明. 茉莉酸甲酯对橡胶树花药愈伤乳管细胞分化的影响. 热带作物学报, 2023 , 44 (2) : 310 -316 . DOI: 10.3969/j.issn.1000-2561.2023.02.010
Zihan GUO, Deguan TAN, Dongyi HUANG, Lili FU, Xuepiao SUN, Jiaming ZHANG. Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli[J]. Chinese Journal of Tropical Crops, 2023 , 44 (2) : 310 -316 . DOI: 10.3969/j.issn.1000-2561.2023.02.010
  • 国家重点研发计划项目(2019YFD1001102)
  • 海南省自然科学基金项目(2019RC296; 322RC761)
2023年第44卷第2期
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doi: 10.3969/j.issn.1000-2561.2023.02.010
  • 接收时间:2022-03-01
  • 首发时间:2026-03-05
  • 出版时间:2023-02-25
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  • 收稿日期:2022-03-01
  • 修回日期:2022-05-08
基金
国家重点研发计划项目(2019YFD1001102)
海南省自然科学基金项目(2019RC296; 322RC761)
作者信息
    1.海南大学热带作物学院,海南海口 570228
    2.中国热带农业科学院热带生物技术研究所/中国热带农业科学院海南热带农业资源研究院/农业农村部热带作物生物学与遗传资源利用重点实验室,海南海口 571101

通讯作者:

* 谭德冠(TAN Deguan),E-mail:
张家明(ZHANG Jiaming),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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