热带作物学报

适合全周期间作的橡胶树品种热垦628花药再生体系研究

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成镜 , 黄天带 ^* , 戴雪梅 , 徐正伟

热带作物学报 | 作物栽培与生理生化 2023,44(9): 1794-1800

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热带作物学报 | 作物栽培与生理生化 2023, 44(9): 1794-1800

适合全周期间作的橡胶树品种热垦628花药再生体系研究

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成镜, 黄天带^*, 戴雪梅, 徐正伟

作者信息

中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地海南省热带作物栽培生理学重点实验室/热带林木种子种苗工程中心/海口市热带植物种苗创新重点实验室，海南海口 571101

成镜（1986—），男，硕士，助理研究员，研究方向：橡胶树等热带林木组织培养快繁。

通讯作者:

^* 黄天带（HUANG Tiandai），E-mail: zhuanjiyinyuzhong@163.com。

Anther Regeneration System of Rubber Tree Variety Reken 628 Suitable for Whole Period Intercropping

Jing CHENG, Tiandai HUANG^*, Xuemei DAI, Zhengwei XU

Affiliations

Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs/State Key Laboratory Incubation Base for Cultivation &Physiology of Tropical Crops/Engineering Center for Rapid Propagation of Tropical Woody Plants, Chinese Academy of Tropical Agricultural Sciences/Haikou Key Laboratory of Innovation of Seedlings of Tropical Plants, Haikou, Hainan 571101, China

出版时间: 2023-09-25 doi: 10.3969/j.issn.1000-2561.2023.09.007

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摘要

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在橡胶树体胚发生再生体系研究中，如何快速获得胚性愈伤组织是能否成功建立再生体系的关键。本研究基于课题组前期的研究基础，以橡胶树花药为外植体，通过正交试验设计方案研究了毒莠定、KT、6-BA、IAA四种激素的不同浓度组合对橡胶树花药胚性愈伤组织诱导的影响，结果表明：橡胶树花药愈伤组织诱导的最优组合培养基为MS基础培养基，硫胺素0.4 mg/L、天冬酰胺300 mg/L、水解酪蛋白100 mg/L、脯氨酸100 mg/L、精氨酸100 mg/L、谷氨酰胺100 mg/L、L-半胱氨酸-盐酸盐50 mg/L、蔗糖70 g/L、椰子水50 mL/L、植物凝胶2.2 g/L，并添加毒莠定浓度20 mg/L、KT浓度2 mg/L、6-BA浓度1 mg/L、IAA浓度0 mg/L，愈伤组织诱导率达73.3%~100%。所有处理在诱导愈伤组织25~28 d左右，愈伤组织达到快速增殖时期，对胚性愈伤组织的诱导起主要作用的是毒莠定浓度，其浓度在20~30 mg/L之间时，对胚性愈伤组织的诱导有促进作用。低浓度的毒莠定虽然也能诱导愈伤组织产生，但是这些愈伤组织几乎为无胚胎发生能力的非胚性愈伤组织。同时用获得的11个体胚进行植株再生，其中出苗2株，7个只长根不抽芽，2个既不抽芽也不长根，植株再生率达18%。因此，在此研究的基础上，对愈伤组织诱导培养基配方进行进一步优化，使其能够更快地从花药中诱导出胚性愈伤组织，缩短胚性愈伤组织的诱导时间，降低变异几率，可为RITA生物反应器提供更好条件的愈伤组织起始原料。

关键词

橡胶树 / 花药 / 胚性愈伤组织 / 植株再生

Abstract

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Quickly obtain embryogenic callus is the key step to successfully establish the regeneration system in the somatic embryogenesis regeneration system of rubber tree. Rubber tree anthers were used as explants in the study. The effects of different concentrations of picloram, KT, 6-BA, and IAA on the induction of rubber tree anther embryogenic callus were studied through an orthogonal experimental design. The results showed that the optimal combination medium for rubber tree anther callus induction was MS basic medium, 0.4 mg/L thiamine, asparagine 300 mg/L, hydrolyzed casein 100 mg/L, proline 100 mg/L, arginine 100 mg/L, glutathione amino amide 100 mg/L, L-cysteine-hydrochloride 50 mg/L, sucrose 70 g/L, coconut water 50 mL/L, vegetable gel 2.2 g/L, picloram 20 mg/L, KT 2 mg/L and KT 2 mg/L and 6-BA 1 mg/L, and the callus induction rate reached 73.3%-100%. In all treatments, the callus was induced for about 25-28 days, and the callus reached the stage of rapid proliferation. The main effect of the induction of embryogenic callus was the concentration of picloram. It could promote the induction of embryogenic callus. Although low concentrations of picloram could also induce callus production, the callus were basically non-embryogenic potential-ie non-embryogenic callus. At the same time, 11 somatic embryos were used for plant regeneration. Among them, two plants emerged, seven only had roots without sprouting, and two had neither buds nor roots. The plant regeneration rate was 18%. By observing the leaf morphology and plant height of the plants, there was no significant difference with the rubber tree tissue culture seedlings and seedlings in production. In the future, on the basis of the research, the formulation of the callus induction medium would be further optimized, so that it could induce embryogenic callus from anthers more quickly, shorten the induction time of embryogenic callus, and reduce variation. Probability, it could provide callus with better conditions for RITA bioreactors as starting material.

Key words

Hevea brasiliensis / anther / embryogenic callus / plant regeneration

引用本文

成镜, 黄天带, 戴雪梅, 徐正伟. 适合全周期间作的橡胶树品种热垦628花药再生体系研究. 热带作物学报, 2023 , 44 (9) : 1794 -1800 . DOI: 10.3969/j.issn.1000-2561.2023.09.007

Jing CHENG, Tiandai HUANG, Xuemei DAI, Zhengwei XU. Anther Regeneration System of Rubber Tree Variety Reken 628 Suitable for Whole Period Intercropping[J]. Chinese Journal of Tropical Crops, 2023 , 44 (9) : 1794 -1800 . DOI: 10.3969/j.issn.1000-2561.2023.09.007

基金

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国家重点研发计划项目(2019YFD1001105)
中央级科研院所基本科研业务费专项(1630022022008)
海南省国际科技合作研发项目(GHYF2022013)

参考文献

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2023年第44卷第9期

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文章信息

doi: 10.3969/j.issn.1000-2561.2023.09.007

接收时间：2022-08-29
首发时间：2026-03-05
出版时间：2023-09-25

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收稿日期：2022-08-29
修回日期：2022-09-15

基金

国家重点研发计划项目(2019YFD1001105)

中央级科研院所基本科研业务费专项(1630022022008)

海南省国际科技合作研发项目(GHYF2022013)

作者信息

通讯作者:

^* 黄天带（HUANG Tiandai），E-mail: zhuanjiyinyuzhong@163.com。

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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.09.007

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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