Article(id=1236292524944445598, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236292518342619367, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.09.019, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1649433600000, receivedDateStr=2022-04-09, revisedDate=1655740800000, revisedDateStr=2022-06-21, acceptedDate=null, acceptedDateStr=null, onlineDate=1772684707150, onlineDateStr=2026-03-05, pubDate=1695571200000, pubDateStr=2023-09-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772684707150, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772684707150, creator=13701087609, updateTime=1772684707150, updator=13701087609, issue=Issue{id=1236292518342619367, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='9', pageStart='1735', pageEnd='1933', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772684705577, creator=13701087609, updateTime=1772695003280, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236335710127575662, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236292518342619367, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236335710127575663, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236292518342619367, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1909, endPage=1916, ext={EN=ArticleExt(id=1236292525217075388, articleId=1236292524944445598, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Cryopreservation of Anthurium andraeanum by Droplet Vitrification, columnId=1236286112713470633, journalTitle=Chinese Journal of Tropical Crops, columnName=Post-harvest Treatment & Quality Safety, runingTitle=null, highlight=null, articleAbstract=

Using the in vitro axillary bud of Anthurium (Anthurium andraeanum Lind.) as the materials, this article reports the influence of the droplet vitrification on Anthurium after cryopreservation, and plants regenerated tissues genetic stability were assessment. The results showed the highest survival rate of Anthurium (63.70%) was achieved after preculture for 4 days on MS solid medium containing 0.4 mmol/L sucrose and 2 mmol/L glycerol, dehydration with 80%PVS2 at 0 ℃ for 50 min, cooling in droplets of PVS2 placed on aluminum foil strips, after immersing in liquid nitrogen for 2 s, directly transferred it to a cryotube filled with liquid nitrogen, and kept it in liquid nitrogen for at least 30 min after rewarming them with MS liquid medium containing 1.2 mmol/L sucrose at room temperature for 20 minutes, transferred to recovery medium. Through ISSR and SSR molecular marker detection, the genetic stability of regenerated plants did not changed. The results would provide an effective way for long-term preservation of Anthurium andraeanum germplasm resources.

, correspAuthors=Li XU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Jie GAO, Zhiying LI, Xuanbing ZHANG, Longhai XIE, Ying CHEN, Zhenfen ZHU, Yunliu FU, Li XU), CN=ArticleExt(id=1236292525649088746, articleId=1236292524944445598, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=红掌小滴玻璃化法超低温保存研究, columnId=1236286112877048492, journalTitle=热带作物学报, columnName=采后处理与质量安全, runingTitle=null, highlight=null, articleAbstract=

以红掌腋芽为试材,探讨小滴玻璃化法对红掌超低温保存的影响因素,并对再生植株进行遗传稳定性检测。结果表明,红掌腋芽置于含0.4 mmol/L蔗糖和2 mmol/L甘油的MS固体培养基中预培养4 d后,在0 ℃下80% PVS2处理50 min,转到铝箔条上PVS2液滴中,将铝箔条迅速浸入液氮中,2 s后直接转入装满液氮的冻存管中,投入液氮至少保持30 min;室温下用含有1.2 mmol/L蔗糖的MS液体培养基复温并卸载20 min后置于恢复培养基上,腋芽存活率高达63.70%。通过ISSR和SSR分子标记检测,再生植株的遗传稳定性未发生改变。该研究结果为红掌种质资源的长期保存提供有效途径。

, correspAuthors=徐立, authorNote=null, correspAuthorsNote=
* 徐立(XU Li),E-mail:
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高洁(1995—),女,硕士,研究方向:种质资源保存。

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高洁(1995—),女,硕士,研究方向:种质资源保存。

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高洁(1995—),女,硕士,研究方向:种质资源保存。

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红掌小滴玻璃化法超低温保存研究
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高洁 1 , 李志英 2 , 张玄兵 1 , 谢龙海 2 , 陈莹 3 , 朱振芬 3 , 符运柳 4 , 徐立 5, *
热带作物学报 | 采后处理与质量安全 2023,44(9): 1909-1916
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热带作物学报 | 采后处理与质量安全 2023, 44(9): 1909-1916
红掌小滴玻璃化法超低温保存研究
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高洁1, 李志英2, 张玄兵1, 谢龙海2, 陈莹3, 朱振芬3, 符运柳4, 徐立5, *
作者信息
  • 1.海南大学,海南海口 570228
  • 2.中国热带农业科学院热带作物品种资源研究所,海南海口 571101
  • 3.华南作物基因资源与种质创制重点实验室,北京 100000
  • 4.海南省热带作物资源遗传改良与创新重点实验室,海南儋州 571737
  • 5.国家热带作物中期库,海南儋州 571737
  • 高洁(1995—),女,硕士,研究方向:种质资源保存。

通讯作者:

* 徐立(XU Li),E-mail:
Cryopreservation of Anthurium andraeanum by Droplet Vitrification
Jie GAO1, Zhiying LI2, Xuanbing ZHANG1, Longhai XIE2, Ying CHEN3, Zhenfen ZHU3, Yunliu FU4, Li XU5, *
Affiliations
  • 1. Hainan University, Haikou, Hainan 570228, China
  • 2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Beijing 100000, China
  • 4. Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Danzhou, Hainan 571737, China
  • 5. National Gene Bank of Tropical Crops, Danzhou, Hainan 571737, China
出版时间: 2023-09-25 doi: 10.3969/j.issn.1000-2561.2023.09.019
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以红掌腋芽为试材,探讨小滴玻璃化法对红掌超低温保存的影响因素,并对再生植株进行遗传稳定性检测。结果表明,红掌腋芽置于含0.4 mmol/L蔗糖和2 mmol/L甘油的MS固体培养基中预培养4 d后,在0 ℃下80% PVS2处理50 min,转到铝箔条上PVS2液滴中,将铝箔条迅速浸入液氮中,2 s后直接转入装满液氮的冻存管中,投入液氮至少保持30 min;室温下用含有1.2 mmol/L蔗糖的MS液体培养基复温并卸载20 min后置于恢复培养基上,腋芽存活率高达63.70%。通过ISSR和SSR分子标记检测,再生植株的遗传稳定性未发生改变。该研究结果为红掌种质资源的长期保存提供有效途径。

红掌  /  小滴玻璃化法  /  超低温保存  /  腋芽  /  遗传稳定性

Using the in vitro axillary bud of Anthurium (Anthurium andraeanum Lind.) as the materials, this article reports the influence of the droplet vitrification on Anthurium after cryopreservation, and plants regenerated tissues genetic stability were assessment. The results showed the highest survival rate of Anthurium (63.70%) was achieved after preculture for 4 days on MS solid medium containing 0.4 mmol/L sucrose and 2 mmol/L glycerol, dehydration with 80%PVS2 at 0 ℃ for 50 min, cooling in droplets of PVS2 placed on aluminum foil strips, after immersing in liquid nitrogen for 2 s, directly transferred it to a cryotube filled with liquid nitrogen, and kept it in liquid nitrogen for at least 30 min after rewarming them with MS liquid medium containing 1.2 mmol/L sucrose at room temperature for 20 minutes, transferred to recovery medium. Through ISSR and SSR molecular marker detection, the genetic stability of regenerated plants did not changed. The results would provide an effective way for long-term preservation of Anthurium andraeanum germplasm resources.

Anthurium andraeanum Lind  /  droplet vitrification  /  cryopreservation  /  axillary buds  /  genetic stability
高洁, 李志英, 张玄兵, 谢龙海, 陈莹, 朱振芬, 符运柳, 徐立. 红掌小滴玻璃化法超低温保存研究. 热带作物学报, 2023 , 44 (9) : 1909 -1916 . DOI: 10.3969/j.issn.1000-2561.2023.09.019
Jie GAO, Zhiying LI, Xuanbing ZHANG, Longhai XIE, Ying CHEN, Zhenfen ZHU, Yunliu FU, Li XU. Cryopreservation of Anthurium andraeanum by Droplet Vitrification[J]. Chinese Journal of Tropical Crops, 2023 , 44 (9) : 1909 -1916 . DOI: 10.3969/j.issn.1000-2561.2023.09.019
  • 中央级科研院所基本科研业务费专项(1630032019039; 1630032020026)
2023年第44卷第9期
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doi: 10.3969/j.issn.1000-2561.2023.09.019
  • 接收时间:2022-04-09
  • 首发时间:2026-03-05
  • 出版时间:2023-09-25
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  • 收稿日期:2022-04-09
  • 修回日期:2022-06-21
基金
中央级科研院所基本科研业务费专项(1630032019039; 1630032020026)
作者信息
    1.海南大学,海南海口 570228
    2.中国热带农业科学院热带作物品种资源研究所,海南海口 571101
    3.华南作物基因资源与种质创制重点实验室,北京 100000
    4.海南省热带作物资源遗传改良与创新重点实验室,海南儋州 571737
    5.国家热带作物中期库,海南儋州 571737

通讯作者:

* 徐立(XU Li),E-mail:
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.09.019
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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