热带作物学报

番石榴叶挥发油的抗菌性及优化提取研究

PDF下载

张晓南 ¹^,² , 王建豪 ² , 王佳丽 ² , 朱鸿维 ² , 牛亚倩 ² , 熊婷婷 ² , 张英迪 ² , 徐福 ²

热带作物学报 | 采后处理与质量安全 2023,44(1): 174-185

收起

热带作物学报 | 采后处理与质量安全 2023, 44(1): 174-185

番石榴叶挥发油的抗菌性及优化提取研究

全屏

张晓南¹^,², 王建豪², 王佳丽², 朱鸿维², 牛亚倩², 熊婷婷², 张英迪², 徐福²

作者信息

1.嘉应学院，广东梅州　541000

2.东北农业大学食品学院，黑龙江哈尔滨　150030

张晓南（1982—），男，博士，副教授，研究方向：功能食品，E-mail: xiaonanzhang@neau.edu.cn。

Antibacterial Activity and Optimal Extraction of Volatile Oil from Guava Leaves

Xiaonan ZHANG¹^,², Jianhao WANG², Jiali WANG², Hongwei ZHU², Yaqian NIU², Tingting XIONG², Yingdi ZHANG², Fu XU²

Affiliations

1. Jiaying University, Meizhou, Guangdong 541000, China

2. College of Food Sciences, Northeast Agricultural University, Harbin, Heilongjiang 150030, China

出版时间: 2023-01-25 doi: 10.3969/j.issn.1000-2561.2023.01.018

文章导航

摘要

收起

分别由酸疮痂链霉菌（Streptomyces acidiscabies, SA）、茄科雷尔氏菌（Ralstonia solanacearum, RS）和胡萝卜软腐欧文氏菌（Erwinia carotovora subsp. carotovora Borgey, ECCB）引起的植物疮痂病、青枯病、软腐病是农作物生产中常见多发的细菌病害。番石榴叶挥发油由多种活性物质组成，具有广谱抗微生物活性。本研究旨在提取并配置番石榴叶挥发油抗菌液，针对酸疮痂链霉菌、茄科雷尔氏菌、胡萝卜软腐欧文氏菌等3种病原菌进行挥发油的抗性研究，为农作物病害生物防治储备资源。采用添加锂盐结合微波辅助水蒸馏法提取番石榴叶挥发油，通过滤纸片扩散法和最小抑制浓度（MIC）评价了番石榴叶挥发油对3种供试菌株的抗菌效果，单因素结合响应面试验优化提取工艺，采用气相色谱-质谱联用技术（GC-MS）测定番石榴叶挥发油的化学成分。结果表明：番石榴叶挥发油对酸疮痂链霉菌、茄科雷尔氏菌、胡萝卜软腐欧文氏菌均表现出良好的抑制效果，抑菌圈直径范围为20.57~23.24 mm，抑菌率均达到60%以上，MIC值分别为3.13、1.56、3.13 μg/mL。挥发油对茄科雷尔氏菌的抑制效果最好，抑菌圈直径为（23.24±0.47）mm，属于高度敏感，对酸疮痂链霉菌、胡萝卜软腐欧文氏菌也具有一定的抑制效果。提取的最佳工艺条件为：液料比10 mL/g（干重）、微波功率660 W、微波时间43 min，在此条件获得的挥发油得率为（10.23±0.17）mL/kg（干重），3次平行试验验证了该方法的有效性和重现性。检测出番石榴叶挥发油的主要成分为萜烯类、醇类等物质，其中β-石竹烯（28.36%）、去氢白菖烯（11.47%）、蓝桉醇（11.31%）、α-蒎烯（10.01%）的含量相对较高；抗菌试验中，发挥主要作用的物质为β-石竹烯、α-蒎烯、丁香酚、D-柠檬烯，推测番石榴叶挥发油对供试菌株的抑菌机理是对其细胞结构造成了破坏，导致细胞内物质泄漏。上述结果表明，番石榴叶挥发油具有良好的抗菌活性以及可观的提取得率，研究结果为番石榴叶的开发应用和农作物病害综合防治提供参考。

关键词

番石榴叶 / 挥发油 / 抗菌性 / 响应面优化 / GC-MS分析

Abstract

收起

Streptomyces acidiscabies (SA), Ralstonia solanacearum (RS) and Erwinia carotovora subsp carotovora borgey (ECCB) cause plant scab, brucellosis and soft rot of carrot, respectively. These are common and prevalent bacterial disease in crop production. The volatile oil of Guava leaves consists of a variety of active substances with broad-spectrum anti-microbial activity. The aim of this study was to extract and configure an antimicrobial solution of guava leaves volatile oil to conduct a volatile oil resistance study against three disease bacteria, SA, RS and ECCB, to reserve resources for biological control agents. The volatile oil of guava leaves was extracted by the addition of lithium salt combined with microwave-assisted hydrodistillation, the antibacterial effect of guava leaf volatile oil on three test strains was evaluated by the filter paper diffusion method and minimum inhibitory concentration (MIC), and the extraction process was optimized by single factor combined with response surface experiments. The chemical components of guava leaf volatile oil were determined by gas chromatography-mass spectrometry (GC-MS). The results showed that the oil exhibited excellent inhibitory effects against SA, RS and ECCB, with the inhibition circle diameters ranging from 20.57 to 23.24 mm and the inhibition rates reaching more than 60%, and the MIC values of 3.13 μg/mL, 1.56 μg/mL and 3.13 μg/mL, respectively. The oil had the greatest inhibitory effect on RS, the diameter of the inhibition circle was (23.24±0.47)mm, which was highly sensitive, and also had certain inhibitory effect on SA and ECCB. The optimum process conditions for the extraction process were liquid to material ratio of 10 mL/g DW, microwave power of 660 W and microwave time of 43 min. The yield of the oil obtained under the conditions was (10.23±0.17)mL/kg DW, and the validity and reproducibility of the method were verified by three parallel experiments. The components of the oil were detected to be mainly terpenes and alcohols, with relatively high content of β-caryophyllene (28.36%), l-calamenene (11.47%), (-)-globulol (11.31%) and α-pinene (10.01%). The substances that played a major role in the antibacterial experiment were β-caryophyllene, α-pinene, eugenol, and D-limonene. It was speculated that the mechanism of guava leaf volatile oil inhibition on the test strain was the damage to its cell structure, which resulting in the leakage of intracellular material. The above results demonstrated that the guava leaf volatile oil has an excellent antimicrobial activity and considerable extraction rate, the results of the study would provide a reference for the development and application of guava leaves and integrated control of crop diseases.

Key words

guava leaf / volatile oil / antibacterial activity / response surface optimization / GC-MS analysis

引用本文

张晓南, 王建豪, 王佳丽, 朱鸿维, 牛亚倩, 熊婷婷, 张英迪, 徐福. 番石榴叶挥发油的抗菌性及优化提取研究. 热带作物学报, 2023 , 44 (1) : 174 -185 . DOI: 10.3969/j.issn.1000-2561.2023.01.018

Xiaonan ZHANG, Jianhao WANG, Jiali WANG, Hongwei ZHU, Yaqian NIU, Tingting XIONG, Yingdi ZHANG, Fu XU. Antibacterial Activity and Optimal Extraction of Volatile Oil from Guava Leaves[J]. Chinese Journal of Tropical Crops, 2023 , 44 (1) : 174 -185 . DOI: 10.3969/j.issn.1000-2561.2023.01.018

基金

收起

梅州市科技专项资金项目(2021B0204007)

参考文献

收起

2023年第44卷第1期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.3969/j.issn.1000-2561.2023.01.018

接收时间：2022-01-25
首发时间：2026-03-05
出版时间：2023-01-25

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2022-01-25
修回日期：2022-04-21

基金

梅州市科技专项资金项目(2021B0204007)

作者信息

1.嘉应学院，广东梅州　541000

2.东北农业大学食品学院，黑龙江哈尔滨　150030

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.01.018

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT