Article(id=1236286117029400959, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236286111962690215, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.01.009, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1646755200000, receivedDateStr=2022-03-09, revisedDate=1650816000000, revisedDateStr=2022-04-25, acceptedDate=null, acceptedDateStr=null, onlineDate=1772683179384, onlineDateStr=2026-03-05, pubDate=1674576000000, pubDateStr=2023-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772683179384, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772683179384, creator=13701087609, updateTime=1772683179384, updator=13701087609, issue=Issue{id=1236286111962690215, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='1', pageStart='1', pageEnd='223', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772683178177, creator=13701087609, updateTime=1772683343075, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236286803662139491, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236286111962690215, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236286803662139492, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236286111962690215, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=83, endPage=91, ext={EN=ArticleExt(id=1236286117390111111, articleId=1236286117029400959, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Metabonomic Analysis of Metabolic Differences in Rucheng Baimaocha Tea Germplasm, columnId=1236256430060261740, journalTitle=Chinese Journal of Tropical Crops, columnName=Germplasm Resources, Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

Rucheng Baimaocha (Camellia sinensis var. pubilimba Chang) is one of the four tea plants native to Hunan Province, China. Previous studies have shown that Rucheng Baimaocha is different from other three local population resources in Hunan in evolutionary status, and has distinctive characteristics in botanical characteristics, biochemical components and tea making quality. However, there are few studies on the differences of secondary metabolites of Rucheng Baimaocha germplasm resources. Using UPLC-MS/MS analysis technology, taking Anhua population (AHQT, C. sinensis var. sinensis), Chengbu Dongcha [CBDC, C. sinensis var. assamica (Masters) Kitamura], and Jianghua Kucha [JHKC, C. sinensis var. assamica (Masters) Kitamura] of typical local tea resources in Hunan as controls, the significantly different metabolites in Rucheng Baimaocha were screened and clustered by K-means analysis and differential metabolite enrichment pathway analysis. Compared with the control, 189, 180 and 162 significantly different metabolites were screened in Rucheng Baimaotea, the most species were flavonoids (69, 51 and 60 respectively), followed by phenolic acids (31, 40 and 25 respectively), and the least were lignans and coumarins (5, 4 and 4 respectively). Compared with the other three populations, Rucheng Baimaotea increased the content of 15 metabolites. The content of flavonoids and phenolic acids increased the most in Rucheng Baimaocha, and the highest increase was pinocembrin, followed by 1,2,3,6-tetra-o-galloyl-d-glucose. The differential metabolites were analyzed by K-means clustering. The metabolites with the same change trend in the four groups could be divided into nine categories. Among the four populations, there were 32 metabolites with the same change trend and the highest content in Rucheng Baimaotea, mainly flavonoids and tannins. The results of KEGG pathway enrichment of differential metabolites showed that the differential metabolites of Rucheng Baimaotea were mainly enriched in flavonoid biosynthesis pathway, flavonoid and flavonol biosynthesis pathway, phenylpropane biosynthesis pathway and so on. In addition to the differential metabolites reported in some tea, some differential metabolites less concerned in tea research were detected, such as pinocembrin, ellagic acid, kaempferol, etc. The substances would provide an important reference for the excavation and breeding of characteristic tea germplasm resources of Rucheng white tea in the later stage.

, correspAuthors=Zhen LIU, authorNote=null, correspAuthorsNote=
*LIU Zhen,E-mail:
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汝城白毛茶是湖南四大茶树地方群体资源之一,也是白毛茶变种(Camellia sinensis var. pubilimba Chang)的典型代表,已有研究表明汝城白毛茶在演化地位上与湖南其他3个地方群体资源差异较大,且在植物学特征、生化成分、制茶品质等方面特色鲜明。但目前对汝城白毛茶种质资源次生代谢物的差异研究较少。本研究采用UPLC-MS/MS分析技术,以汝城白毛茶为研究对象,湖南典型地方茶树资源安化群体(AHQT, C. sinensis var. sinensis)、城步峒茶[CBDC, C. sinensis var. assamica (Masters) Kitamura]、江华苦茶[JHKC, C. sinensis var. assamica (Masters) Kitamura]为对照,筛选汝城白毛茶中的显著差异代谢物,并进行K均值聚类(K-means)分析和差异代谢物富集通路分析。结果表明,与AHQT、CBDC、JHKC相比,分别在汝城白毛茶中筛选到显著差异代谢物189、180、162个,种类最多的为黄酮类(69、51、60个),其次为酚酸类(31、40、25个),最少的为木脂素和香豆素(5、4、4个)。汝城白毛茶较对照量均增加的代谢物共15个,含量增加种类最多的均为黄酮和酚酸类,其中增加最多的均为乔松素、其次为1,2,3,6-四-O-没食子酰-D-葡萄糖;将差异代谢物进行K均值聚类(K-means)分析,4个群体中变化趋势一致的代谢物可分为9类,且在汝城白毛茶中含量最高的代谢物共有32个,主要为黄酮和鞣质类。差异代谢物的KEGG通路富集结果表明,汝城白毛茶的差异代谢物主要富集于类黄酮生物合成途径、黄酮与黄酮醇生物合成途径、苯丙烷生物合成途径等。本研究除检测到一些茶叶中已有研究报道的差异代谢物外,筛选出了一些茶叶研究中关注较少的差异代谢物,如乔松素、鞣花酸、山奈酚等,这些物质将为后期汝城白毛茶特色茶树种质资源的挖掘和选育研究提供重要参考。

, correspAuthors=刘振, authorNote=null, correspAuthorsNote=
*刘振,E-mail:
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赵熙(1982—),女,硕士,副研究员,研究方向:茶叶加工与品质化学。

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赵熙(1982—),女,硕士,副研究员,研究方向:茶叶加工与品质化学。

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赵熙(1982—),女,硕士,副研究员,研究方向:茶叶加工与品质化学。

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茶树种质资源汝城白毛茶的代谢物差异研究
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赵熙 , 赵洋 , 杨培迪 , 宁静 , 杨阳 , 成杨 , 刘振 *
热带作物学报 | 种质资源与遗传育种 2023,44(1): 83-91
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热带作物学报 | 种质资源与遗传育种 2023, 44(1): 83-91
茶树种质资源汝城白毛茶的代谢物差异研究
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赵熙, 赵洋, 杨培迪, 宁静, 杨阳, 成杨, 刘振*
作者信息
  • 湖南省农业科学院茶叶研究所/国家茶树改良中心湖南分中心/湖南省茶树品种与种苗工程技术研究中心,湖南长沙 410125
  • 赵熙(1982—),女,硕士,副研究员,研究方向:茶叶加工与品质化学。

通讯作者:

*刘振,E-mail:
Metabonomic Analysis of Metabolic Differences in Rucheng Baimaocha Tea Germplasm
Xi ZHAO, Yang ZHAO, Peidi YANG, Jing NING, Yang YANG, Yang CHENG, Zhen LIU*
Affiliations
  • Tea Research Institute, Hunan Academy of Agricultural Sciences/National Center for Tea Improvement in Hunan Branch/Hunan Tea Plant Variety and Seedling Engineering Technology Research Center, Changsha, Hunan 410125, China
出版时间: 2023-01-25 doi: 10.3969/j.issn.1000-2561.2023.01.009
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汝城白毛茶是湖南四大茶树地方群体资源之一,也是白毛茶变种(Camellia sinensis var. pubilimba Chang)的典型代表,已有研究表明汝城白毛茶在演化地位上与湖南其他3个地方群体资源差异较大,且在植物学特征、生化成分、制茶品质等方面特色鲜明。但目前对汝城白毛茶种质资源次生代谢物的差异研究较少。本研究采用UPLC-MS/MS分析技术,以汝城白毛茶为研究对象,湖南典型地方茶树资源安化群体(AHQT, C. sinensis var. sinensis)、城步峒茶[CBDC, C. sinensis var. assamica (Masters) Kitamura]、江华苦茶[JHKC, C. sinensis var. assamica (Masters) Kitamura]为对照,筛选汝城白毛茶中的显著差异代谢物,并进行K均值聚类(K-means)分析和差异代谢物富集通路分析。结果表明,与AHQT、CBDC、JHKC相比,分别在汝城白毛茶中筛选到显著差异代谢物189、180、162个,种类最多的为黄酮类(69、51、60个),其次为酚酸类(31、40、25个),最少的为木脂素和香豆素(5、4、4个)。汝城白毛茶较对照量均增加的代谢物共15个,含量增加种类最多的均为黄酮和酚酸类,其中增加最多的均为乔松素、其次为1,2,3,6-四-O-没食子酰-D-葡萄糖;将差异代谢物进行K均值聚类(K-means)分析,4个群体中变化趋势一致的代谢物可分为9类,且在汝城白毛茶中含量最高的代谢物共有32个,主要为黄酮和鞣质类。差异代谢物的KEGG通路富集结果表明,汝城白毛茶的差异代谢物主要富集于类黄酮生物合成途径、黄酮与黄酮醇生物合成途径、苯丙烷生物合成途径等。本研究除检测到一些茶叶中已有研究报道的差异代谢物外,筛选出了一些茶叶研究中关注较少的差异代谢物,如乔松素、鞣花酸、山奈酚等,这些物质将为后期汝城白毛茶特色茶树种质资源的挖掘和选育研究提供重要参考。

茶树  /  汝城白毛茶  /  代谢组学  /  液质联用  /  代谢物

Rucheng Baimaocha (Camellia sinensis var. pubilimba Chang) is one of the four tea plants native to Hunan Province, China. Previous studies have shown that Rucheng Baimaocha is different from other three local population resources in Hunan in evolutionary status, and has distinctive characteristics in botanical characteristics, biochemical components and tea making quality. However, there are few studies on the differences of secondary metabolites of Rucheng Baimaocha germplasm resources. Using UPLC-MS/MS analysis technology, taking Anhua population (AHQT, C. sinensis var. sinensis), Chengbu Dongcha [CBDC, C. sinensis var. assamica (Masters) Kitamura], and Jianghua Kucha [JHKC, C. sinensis var. assamica (Masters) Kitamura] of typical local tea resources in Hunan as controls, the significantly different metabolites in Rucheng Baimaocha were screened and clustered by K-means analysis and differential metabolite enrichment pathway analysis. Compared with the control, 189, 180 and 162 significantly different metabolites were screened in Rucheng Baimaotea, the most species were flavonoids (69, 51 and 60 respectively), followed by phenolic acids (31, 40 and 25 respectively), and the least were lignans and coumarins (5, 4 and 4 respectively). Compared with the other three populations, Rucheng Baimaotea increased the content of 15 metabolites. The content of flavonoids and phenolic acids increased the most in Rucheng Baimaocha, and the highest increase was pinocembrin, followed by 1,2,3,6-tetra-o-galloyl-d-glucose. The differential metabolites were analyzed by K-means clustering. The metabolites with the same change trend in the four groups could be divided into nine categories. Among the four populations, there were 32 metabolites with the same change trend and the highest content in Rucheng Baimaotea, mainly flavonoids and tannins. The results of KEGG pathway enrichment of differential metabolites showed that the differential metabolites of Rucheng Baimaotea were mainly enriched in flavonoid biosynthesis pathway, flavonoid and flavonol biosynthesis pathway, phenylpropane biosynthesis pathway and so on. In addition to the differential metabolites reported in some tea, some differential metabolites less concerned in tea research were detected, such as pinocembrin, ellagic acid, kaempferol, etc. The substances would provide an important reference for the excavation and breeding of characteristic tea germplasm resources of Rucheng white tea in the later stage.

tea plant  /  Rucheng Baimaocha  /  metabolomics  /  LC-MS  /  metabolites
赵熙, 赵洋, 杨培迪, 宁静, 杨阳, 成杨, 刘振. 茶树种质资源汝城白毛茶的代谢物差异研究. 热带作物学报, 2023 , 44 (1) : 83 -91 . DOI: 10.3969/j.issn.1000-2561.2023.01.009
Xi ZHAO, Yang ZHAO, Peidi YANG, Jing NING, Yang YANG, Yang CHENG, Zhen LIU. Metabonomic Analysis of Metabolic Differences in Rucheng Baimaocha Tea Germplasm[J]. Chinese Journal of Tropical Crops, 2023 , 44 (1) : 83 -91 . DOI: 10.3969/j.issn.1000-2561.2023.01.009
  • 国家茶叶产业技术体系项目(CARS-19)
  • 湖南省农业科技创新资金项目(2021CX26)
2023年第44卷第1期
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doi: 10.3969/j.issn.1000-2561.2023.01.009
  • 接收时间:2022-03-09
  • 首发时间:2026-03-05
  • 出版时间:2023-01-25
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  • 收稿日期:2022-03-09
  • 修回日期:2022-04-25
基金
国家茶叶产业技术体系项目(CARS-19)
湖南省农业科技创新资金项目(2021CX26)
作者信息
    湖南省农业科学院茶叶研究所/国家茶树改良中心湖南分中心/湖南省茶树品种与种苗工程技术研究中心,湖南长沙 410125

通讯作者:

*刘振,E-mail:
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.01.009
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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