Article(id=1236256431763149277, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236256428533535066, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.11.006, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1654358400000, receivedDateStr=2022-06-05, revisedDate=1663171200000, revisedDateStr=2022-09-15, acceptedDate=null, acceptedDateStr=null, onlineDate=1772676101866, onlineDateStr=2026-03-05, pubDate=1700841600000, pubDateStr=2023-11-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772676101866, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772676101866, creator=13701087609, updateTime=1772676101866, updator=13701087609, issue=Issue{id=1236256428533535066, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='11', pageStart='2135', pageEnd='2353', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772676101096, creator=13701087609, updateTime=1772703795208, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236372586125390480, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236256428533535066, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236372586125390481, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236256428533535066, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=2188, endPage=2195, ext={EN=ArticleExt(id=1236256434011296307, articleId=1236256431763149277, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Selection of Suitable RT-qPCR Reference Genes for Floral Scent Biosynthesis in Phalaenopsis I-Hsin Venus, columnId=1236256431436002236, journalTitle=Chinese Journal of Tropical Crops, columnName=Special Topic of Orchid Science, runingTitle=null, highlight=null, articleAbstract=

Phalaenopsis I-Hsin Venus is an excellent variety of fragrant flowers, with elegant flower types, long flowering period and rich floral fragrance, which has high garden ornamental value. ACT1, ACT2, ACT3, GAPDH, EF1α, TUA, TUB and Ubi, were selected as the candidate internal reference genes based on the transcriptome data of different flower development stages in Phal. I-Hsin Venus to select the appropriate reference genes for RT-qPCR analysis of the correlated genes in the biosynthesis pathway of the floral scent in Phalaenopsis I-Hsin Venus. The expression of the candidate internal reference genes in inflorescences at different stages was detected by RT-qPCR. The candidate internal parameter genes were analyzed by combining three internal parameter gene stability analysis software: geNorm, NormFinder and BestKeeper. The results of comprehensive analysis showed that ACT1 was the most stable and could be used as the best internal reference gene for the expression analysis of Phal. I-Hsin Venus.

, correspAuthors=Yuzhen ZHOU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yangting ZHANG, Yanping ZHANG, Jingyan HUANG, Wenjun WANG, Yan TONG, Kai ZHAO, Yuzhen ZHOU), CN=ArticleExt(id=1236256434321674833, articleId=1236256431763149277, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=一心维纳斯蝴蝶兰花香物质合成相关基因RT-qPCR内参基因筛选, columnId=1236256432996283328, journalTitle=热带作物学报, columnName=兰花科学专题, runingTitle=null, highlight=null, articleAbstract=

一心维纳斯蝴蝶兰（Phalaenopsis I-Hsin Venus）花型优雅、花期持久、花香馥郁，是优良的香花品种，具有较高的园林观赏价值和经济价值。为筛选适用于一心维纳斯蝴蝶兰花香物质合成相关基因表达分析的内参基因，本研究以一心维纳斯蝴蝶兰不同花期（中蕾期、初开期、盛开前期、盛开中期、盛开后期、衰败期）的转录组数据为基础，选取了ACT1、ACT2、ACT3、GAPDH、EF1α、TUA、TUB和Ubi共8个管家基因作为候选内参基因，利用实时荧光定量PCR（RT-qPCR）技术检测候选内参基因在花朵不同发育时期的表达情况，并利用geNorm、NormFinder、BestKeeper三个内参基因稳定性分析软件对候选内参基因进行分析。综合分析结果表明，ACT1的稳定性最高，可作为一心维纳斯蝴蝶兰相关基因表达分析的最适内参基因。

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章杨婷（1998—），女，硕士研究生，研究方向：园林植物与应用。

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章杨婷（1998—），女，硕士研究生，研究方向：园林植物与应用。

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