热带作物学报

基于广泛靶向代谢组学技术的不同花色秋石斛中花青素差异分析

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武美卿 ¹^,² , 廖易 ²^,³ , 陆顺教 ²^,³ , 殷涵泰 ¹^,² , 余文刚 ¹^,^* , 李崇晖 ²^,³^,^*

热带作物学报 | 兰花科学专题 2023,44(11): 2167-2178

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热带作物学报 | 兰花科学专题 2023, 44(11): 2167-2178

基于广泛靶向代谢组学技术的不同花色秋石斛中花青素差异分析

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武美卿¹^,², 廖易²^,³, 陆顺教²^,³, 殷涵泰¹^,², 余文刚¹^,^*, 李崇晖²^,³^,^*

作者信息

^1.海南大学热带农林学院，海南海口　570228

^2.中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室/海南省热带作物资源遗传改良与创新重点实验室，海南海口　571101

^3.海南省热带观赏植物种质创新利用工程技术研究中心，海南儋州　571737

武美卿（1999—），女，硕士研究生，研究方向：观赏园艺学。

通讯作者:

^* 李崇晖（LI Chonghui），E-mail: blchh@sina.com;

余文刚（YU Wengang），E-mail: yuwg2008@hainanu.edu.cn。

Metabolomics Analysis of Anthocyanin in Different Flower Colors of Phalaenopsis-Type Dendrobium

Meiqing WU¹^,², Yi LIAO²^,³, Shunjiao LU²^,³, Hantai YIN¹^,², Wengang YU¹^,^*, Chonghui LI²^,³^,^*

Affiliations

^1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

^2.Tropical Crop Germplasm Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, Hainan 571101, China

^3.Hainan Provincial Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, Hainan 571737, China

出版时间: 2023-11-25 doi: 10.3969/j.issn.1000-2561.2023.11.004

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摘要

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为了深入研究不同花色秋石斛品种花青素合成途径的差异性，本研究利用广泛靶向代谢组学技术对蓝紫色、浅桃红色、红色、紫红色和深紫红色5个不同花色秋石斛品种的花青素苷组成和含量进行了分析。结果表明：从供试秋石斛中共鉴定出38个代谢产物，主要为矢车菊素（Cy）、芍药花素（Pn）、天竺葵素（Pg）、飞燕草素（Dp）和矮牵牛素（Pt）的糖苷及其酰基化衍生物。5个秋石斛品种中花青素苷组成和含量存在显著差异。代谢物差异分析表明，紫红色花中Cy和Dp型糖苷显著高于浅桃红色花（P<0.05）；蓝紫色花的差异花青素中3种Dp型糖苷含量均显著高于其他颜色；仅筛选出1种Cy型糖苷在浅桃红色花中富集。绝大多数样品的Cy型糖苷含量最高，随着Cy和Dp及其衍生物含量增加，花色会加深并转向红紫色，推测Cy型糖苷使秋石斛偏向紫红色，而Dp糖苷赋予秋石斛蓝紫色调。基于以上结果推测秋石斛存在上述5种花青素苷的酰基化修饰途径，研究结果可为秋石斛花色形成机制和花色改良提供依据。

关键词

秋石斛 / 花色 / 代谢组 / 花青素

Abstract

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In order to further investigate the anthocyanin biosynthetic pathway in Phalaenopsis-type Dendrobium (Den-Phals) varieties with different flower color, this study used widely targeted metabolomics technology to analyze the anthocyanin composition and content of five Den-Phals varieties with blue-purple, light peach-red, red, purple-red and deep purple-red floral color, respectively. The results showed that a total of 38 metabolites were identified from the tested Den-Phals, most of which were glycosides and acylated derivatives of cyanidin (Cy), peonidin (Pn), pelargonidin (Pg), delphinidin (Dp) and petunidin (Pt). There were significant differences in the composition and content of anthocyanin in five Den-Phals varieties. Metabolite difference analysis showed that Cy and Dp glycosides in purple flowers were significantly higher than those in light peach red flowers. The contents of three Dp-type glycosides in blue-purple flowers were significantly higher than those in other colored varieties. Only one Cy-type glycoside was screened out, and the content of which in light pink flowers was high. The content of Cy-type glycosides was the highest in most samples. With the increase of cyanidin and delphinidin and the derivatives, the color of flowers deepened and turned to red-purple. It was speculated that Cy-type glycosides made Den-Phals tend to purple while Dp glycosides gave Den-Phals a blue-purple tone. Based on the above results, it is speculated that there are acylation synthesis pathways of the above five anthocyanins in Den-Phals, which can provide a basis for flower color formation mechanism and flower color improvement of Den-Phals.

Key words

Phalaenopsis-type Dendrobium / flower color / metabolome / anthocyanins

引用本文

武美卿, 廖易, 陆顺教, 殷涵泰, 余文刚, 李崇晖. 基于广泛靶向代谢组学技术的不同花色秋石斛中花青素差异分析. 热带作物学报, 2023 , 44 (11) : 2167 -2178 . DOI: 10.3969/j.issn.1000-2561.2023.11.004

Meiqing WU, Yi LIAO, Shunjiao LU, Hantai YIN, Wengang YU, Chonghui LI. Metabolomics Analysis of Anthocyanin in Different Flower Colors of Phalaenopsis-Type Dendrobium[J]. Chinese Journal of Tropical Crops, 2023 , 44 (11) : 2167 -2178 . DOI: 10.3969/j.issn.1000-2561.2023.11.004

基金

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中央级公益性科研院所基本科研业务费专项(1630032022002)
海南省自然科学基金高层次人才项目(321RC 648)

参考文献

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2023年第44卷第11期

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文章信息

doi: 10.3969/j.issn.1000-2561.2023.11.004

接收时间：2023-07-26
首发时间：2026-03-05
出版时间：2023-11-25

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出版历史

收稿日期：2023-07-26
修回日期：2023-09-12

基金

中央级公益性科研院所基本科研业务费专项(1630032022002)

海南省自然科学基金高层次人才项目(321RC 648)

作者信息

^1.海南大学热带农林学院，海南海口　570228

^3.海南省热带观赏植物种质创新利用工程技术研究中心，海南儋州　571737

通讯作者:

^* 李崇晖（LI Chonghui），E-mail: blchh@sina.com;

余文刚（YU Wengang），E-mail: yuwg2008@hainanu.edu.cn。

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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.11.004

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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