Article(id=1277255495811461882, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.03.016, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1760544000000, receivedDateStr=2025-10-16, revisedDate=null, revisedDateStr=null, acceptedDate=1766160000000, acceptedDateStr=2025-12-20, onlineDate=1782451040461, onlineDateStr=2026-06-26, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782451040461, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782451040461, creator=13701087609, updateTime=1782451040461, updator=13701087609, issue=Issue{id=1277255461552390652, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='3', pageStart='553', pageEnd='823', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782451032293, creator='13701087609', updateTime=1782451557265, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257664249852732, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257664249852733, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=717, endPage=730, ext={EN=ArticleExt(id=1277255496583213820, articleId=1277255495811461882, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Investigation and Pathogen Biological Characteristics of Konjac Soft Rot in Hainan, columnId=1236292524264968282, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Protection & Bio-safety, runingTitle=null, highlight=null, articleAbstract=

Konjac (Amorphophallus spp.) is an important medicine and food economic crop in China, but its industrial development has long been threatened by soft rot disease, known as the konjac 'cancer'. Hainan is an emerging konjac producing area, but the current epidemiological pattern of konjac soft rot disease and pathogenic bacteria population structure is not clarified. The study was aimed to carry out soft rot disease investigation, pathogen isolation and identification, and to provide a scientific basis for the control of the disease. The survey of konjac soft rot disease in six cities and counties in Hainan showed that the highest incidence rate (46.01%) was found in the third team of Danzhou Experimental Farm, while that of Wenchang planting base is low (8.25%). Combining morphology, molecular biology (16S rDNA, proA, gapA and mdh multigene phylogenetic analysis) identifications and distribution information of pathogens, Dickeya fangzhongdai (88.64% of the total), Pectobacterium aroidearum (6.82%) and P. colocasium (4.54%), were the cause of the disease. Pathogenicity assay showed that D. fangzhongdai was high pathogenicity, and indoor screening showed that 0.3% tetramycin had the best inhibitory effect on D. fangzhongdai, with the lowest inhibitory concentration (MIC) of 1.825 μg/mL, followed by 80% ethacrynicin (MIC of 25 μg/mL) and 25% bromoxynil (MIC of 125 μg/mL). The dynamic changes of defence antioxidant enzymes and malondialdehyde in konjac in response to D. fangzhongdai infestation were determined by pot inoculation experiment. The results showed that the activity of superoxide dismutase (SOD) and peroxidase (POD) reached 182.57 U/g and 300.33 U/g, respectively, after 72 h of infestation, which was 2.27-fold and 7.03-fold higher than that of the control, while the activity of catalase (CAT) peaked at 24 h (1093.67 U/g) and decreased to 658.01 U/g in the later period (72 h). Malondialdehyde (MDA) content increased incrementally with infestation time and increased by 30.51% at 72 h compared to the control, and β-galactosidase (β-GAL) activity continued to rise [97 nmol/(min·g)] and increased by 36.86% compared to the control.

, authors=null, authorsList=Xiaolan ZHENG, Lei LUO, Kexian YI, Weihuai WU, Yanqiong LIANG, Shibei TAN, authorCompany=null, correspAuthors=Kexian YI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277255497409491710, articleId=1277255495811461882, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=海南魔芋软腐病病害调查及病原生物学特性研究, columnId=1236292524520820846, journalTitle=热带作物学报, columnName=植物保护与生物安全, runingTitle=null, highlight=null, articleAbstract=

魔芋（Amorphophallus spp．）是我国重要的药食同源经济作物，然而其产业发展长期受软腐病威胁，魔芋软腐病被称为魔芋的“癌症”。海南作为新兴魔芋种植区，目前对魔芋软腐病病害流行规律及病原菌种群结构尚未明确，严重制约产业可持续发展。本研究开展海南魔芋软腐病病害调查和病原菌分离鉴定，旨在为海南魔芋软腐病高效绿色防控提供科学依据。对海南6个市（县）魔芋软腐病病害进行调查，结果表明：儋州试验场三队发病率最高（46.01%），文昌种植基地较低（8.25%）。结合形态学、分子生物学（16S rDNA、proA、gapA和mdh多基因系统发育分析）鉴定及病原菌分布调查，发现病原菌为方中达迪基氏菌（Dickeya fangzhongdai，占88.64%）、环状果胶杆菌（Pectobacterium aroidearum，占6.82%）和芋头果胶杆菌（P．colocasium，占4.54%）。致病力测定显示，D．fangzhongdai的致病力普遍高于其他病原菌；室内药剂筛选表明，0.3%四霉素对D．fangzhongdai的抑制效果最佳，最低抑制浓度（MIC）为1.825 μg/mL，80%乙蒜素（MIC为25 μg/mL）和25%溴菌腈（MIC为125 μg/mL）次之。通过盆栽接种试验测定魔芋响应D．fangzhongdai侵染过程中相关防御性抗氧化酶与丙二醛的动态变化，结果表明，侵染72 h后，超氧化物歧化酶（SOD）和过氧化物酶（POD）活性分别达182.57 U/g和300.33 U/g，分别比对照提高2.27倍和7.03倍；过氧化氢酶（CAT）活性在24 h达峰值（1093.67 U/g），后期降至658.01 U/g（72 h）；丙二醛（MDA）含量随侵染时间递增，72 h时较对照增加30.51%，β-半乳糖苷酶（β-GAL）活性持续上升［97 nmol/（min·g）］，比对照组增加36.86%。

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郑肖兰（1976—），女，硕士，副研究员，研究方向：植物病理学；

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郑肖兰（1976—），女，硕士，副研究员，研究方向：植物病理学；

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