Article(id=1277255492351165065, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.03.006, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1762012800000, receivedDateStr=2025-11-02, revisedDate=null, revisedDateStr=null, acceptedDate=1764000000000, acceptedDateStr=2025-11-25, onlineDate=1782451039635, onlineDateStr=2026-06-26, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782451039635, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782451039635, creator=13701087609, updateTime=1782451039635, updator=13701087609, issue=Issue{id=1277255461552390652, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='3', pageStart='553', pageEnd='823', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782451032293, creator='13701087609', updateTime=1782451557265, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257664249852732, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257664249852733, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=613, endPage=622, ext={EN=ArticleExt(id=1277255492728652427, articleId=1277255492351165065, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Construction of Core Germplasm of Mango Based on SSR Fluorescent Markers, columnId=1236256430060261740, journalTitle=Chinese Journal of Tropical Crops, columnName=Germplasm Resources, Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

Germplasm resources are the key material support for the breeding of new mango varieties and the sustainable development of the industry, and the efficient conservation and utilization are of great significance for mango research. In the study, 431 mango germplasm accessions were used. Combining different sampling strategies and genetic distances, the optimal sampling strategy combination for constructing the mango core collection was screened by the UPGMA clustering method, and the core collection was subsequently established. The results showed that the average effective number of alleles (Ne), average Shannon's information index (I), and average Nei's diversity index (H) of 12 pairs of primers was 4.0993, 1.534 and 0.7361, respectively, indicating that the mango germplasm possessed rich genetic diversity. The t-test results of genetic diversity parameters revealed that the core collection constructed by the combination of the locus priority sampling strategy and Nei & Li genetic distance had the highest Ne, H and I among all tested groups, with value of 4.2603, 0.7486 and 1.5855, respectively, demonstrating that the combination was the optimal method for constructing the mango core collection. The established core collection comprised 85 accessions, accounting for 19.72% of the original germplasm. The retention rate of Ne, I and H in the core collection reached 103.93%, 103.35% and 101.45%, respectively, indicating that although the core collection was much smaller in quantity than the original germplasm, it exhibited superior performance in genetic diversity-related indicators. Principal coordinate analysis (PCoA) showed that the distribution of the core collection uniformly covered the distribution range of the original germplasm in the principal coordinate space, suggesting that the core collection could comprehensively retain the genetic diversity characteristics of the original germplasm and had good representativeness.

, authors=null, authorsList=Yujuan TANG, Ying ZHAO, Enliang SONG, Yu ZHANG, Limei GUO, Guodi HUANG, Xiang LI, Shixing LUO, authorCompany=null, correspAuthors=Xiang LI, Shixing LUO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277255493131305613, articleId=1277255492351165065, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=基于SSR荧光标记的杧果核心种质构建, columnId=1236256430219645304, journalTitle=热带作物学报, columnName=种质资源与遗传育种, runingTitle=null, highlight=null, articleAbstract=

种质资源是杧果新品种培育与产业持续发展的关键物质支撑,其高效保存与利用对杧果研究具有重要意义。本研究以431份杧果种质为材料,结合不同取样策略和遗传距离,通过UPGMA聚类法筛选杧果核心种质构建的最优取样策略组合,并构建核心种质。结果显示:12对引物的平均有效等位基因数(Ne)为4.0993、平均Shannon's信息指数(I)为1.534、平均Nei's多样性指数(H)为0.7361,表明杧果种质具有丰富的遗传多样性。遗传多样性参数t检验结果表明,位点优先取样策略与Nei & Li遗传距离组合构建的核心种质中,Ne、HI在所有测试群体中均最高,分别为4.2603、0.7486和1.5855,说明该组合是构建杧果核心种质的最优方法;构建的核心种质包含85份种质,占原种质比例的19.72%,其Ne、IH的保留率分别达103.93%、103.35%、101.45%,说明核心种质虽在数量上远少于原种质,但在遗传多样性相关指标上表现更优。主坐标轴分析显示,核心种质的分布较均匀地覆盖了原种质在主坐标空间中的分布范围,表明核心种质能够较为全面地保留原种质的遗传多样性特征,具有良好的代表性。

, authors=

* 赵英(1980—),女,硕士,高级农艺师,研究方向:杧果遗传育种。

唐玉娟(1987—),女,硕士,助理研究员,研究方向:杧果遗传育种;

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** 罗世杏(LUO Shixing),E-mail:
李翔(LIXiang),E-mail:
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唐玉娟(1987—),女,硕士,助理研究员,研究方向:杧果遗传育种;

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唐玉娟(1987—),女,硕士,助理研究员,研究方向:杧果遗传育种;

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基于SSR荧光标记的杧果核心种质构建
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唐玉娟 , 赵英 , 宋恩亮 , 张宇 , 郭丽梅 , 黄国弟 , 李翔 **, ** , 罗世杏 **, **
热带作物学报 | 种质资源与遗传育种 2026,47(3): 613-622
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热带作物学报 |种质资源与遗传育种 2026 , 47 (3) : 613 -622
基于SSR荧光标记的杧果核心种质构建
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唐玉娟, 赵英, 宋恩亮, 张宇, 郭丽梅, 黄国弟, 李翔**, ** , 罗世杏**, **
作者信息
  • 广西亚热带作物研究所/广西杧果产业绿色高效发展工程研究中心/广西亚热带特色水果质量安全控制重点实验室,广西南宁 530001
通讯作者:
** 罗世杏(LUO Shixing),E-mail:
李翔(LIXiang),E-mail:
Construction of Core Germplasm of Mango Based on SSR Fluorescent Markers
Yujuan TANG, Ying ZHAO, Enliang SONG, Yu ZHANG, Limei GUO, Guodi HUANG, Xiang LI , Shixing LUO
Affiliations
  • Guangxi Subtropical Crops Research Institute/Guangxi Engineering Research Center of Green and Efficient Development for Mango Industry / Guangxi Key Laboratory of Quality and Safety Control for Subtropical Fruits, Nanning, Guangxi 530001, China
出版时间: 2026-03-25 doi: 10.3969/j.issn.1000-2561.2026.03.006
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种质资源是杧果新品种培育与产业持续发展的关键物质支撑,其高效保存与利用对杧果研究具有重要意义。本研究以431份杧果种质为材料,结合不同取样策略和遗传距离,通过UPGMA聚类法筛选杧果核心种质构建的最优取样策略组合,并构建核心种质。结果显示:12对引物的平均有效等位基因数(Ne)为4.0993、平均Shannon's信息指数(I)为1.534、平均Nei's多样性指数(H)为0.7361,表明杧果种质具有丰富的遗传多样性。遗传多样性参数t检验结果表明,位点优先取样策略与Nei & Li遗传距离组合构建的核心种质中,Ne、HI在所有测试群体中均最高,分别为4.2603、0.7486和1.5855,说明该组合是构建杧果核心种质的最优方法;构建的核心种质包含85份种质,占原种质比例的19.72%,其Ne、IH的保留率分别达103.93%、103.35%、101.45%,说明核心种质虽在数量上远少于原种质,但在遗传多样性相关指标上表现更优。主坐标轴分析显示,核心种质的分布较均匀地覆盖了原种质在主坐标空间中的分布范围,表明核心种质能够较为全面地保留原种质的遗传多样性特征,具有良好的代表性。

杧果  /  SSR  /  核心种质  /  位点优先取样策略  /  遗传距离

Germplasm resources are the key material support for the breeding of new mango varieties and the sustainable development of the industry, and the efficient conservation and utilization are of great significance for mango research. In the study, 431 mango germplasm accessions were used. Combining different sampling strategies and genetic distances, the optimal sampling strategy combination for constructing the mango core collection was screened by the UPGMA clustering method, and the core collection was subsequently established. The results showed that the average effective number of alleles (Ne), average Shannon's information index (I), and average Nei's diversity index (H) of 12 pairs of primers was 4.0993, 1.534 and 0.7361, respectively, indicating that the mango germplasm possessed rich genetic diversity. The t-test results of genetic diversity parameters revealed that the core collection constructed by the combination of the locus priority sampling strategy and Nei & Li genetic distance had the highest Ne, H and I among all tested groups, with value of 4.2603, 0.7486 and 1.5855, respectively, demonstrating that the combination was the optimal method for constructing the mango core collection. The established core collection comprised 85 accessions, accounting for 19.72% of the original germplasm. The retention rate of Ne, I and H in the core collection reached 103.93%, 103.35% and 101.45%, respectively, indicating that although the core collection was much smaller in quantity than the original germplasm, it exhibited superior performance in genetic diversity-related indicators. Principal coordinate analysis (PCoA) showed that the distribution of the core collection uniformly covered the distribution range of the original germplasm in the principal coordinate space, suggesting that the core collection could comprehensively retain the genetic diversity characteristics of the original germplasm and had good representativeness.

mango  /  SSR  /  core germplasm  /  locus-priority sampling strategy  /  genetic distance
唐玉娟, 赵英, 宋恩亮, 张宇, 郭丽梅, 黄国弟, 李翔, 罗世杏. 基于SSR荧光标记的杧果核心种质构建. 热带作物学报, 2026 , 47 (3) : 613 -622 . DOI: 10.3969/j.issn.1000-2561.2026.03.006
Yujuan TANG, Ying ZHAO, Enliang SONG, Yu ZHANG, Limei GUO, Guodi HUANG, Xiang LI, Shixing LUO. Construction of Core Germplasm of Mango Based on SSR Fluorescent Markers[J]. Chinese Journal of Tropical Crops, 2026 , 47 (3) : 613 -622 . DOI: 10.3969/j.issn.1000-2561.2026.03.006
  • 广西自然科学基金项目(2021GXNSFBA196011)
  • 广西农业科学院基本科研业务专项(桂农科2025YP132)
  • 广西创新驱动发展专项(桂科AA22068098-1)
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doi: 10.3969/j.issn.1000-2561.2026.03.006
  • 接收时间:2025-11-02
  • 首发时间:2026-06-26
  • 出版时间:2026-03-25
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  • 收稿日期:2025-11-02
  • 录用日期:2025-11-25
基金
广西自然科学基金项目(2021GXNSFBA196011)
广西农业科学院基本科研业务专项(桂农科2025YP132)
广西创新驱动发展专项(桂科AA22068098-1)
作者信息
    广西亚热带作物研究所/广西杧果产业绿色高效发展工程研究中心/广西亚热带特色水果质量安全控制重点实验室,广西南宁 530001

通讯作者:

** 罗世杏(LUO Shixing),E-mail:
李翔(LIXiang),E-mail:
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2026.03.006
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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