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Article(id=1277255463477579841, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.03.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1763395200000, receivedDateStr=2025-11-18, revisedDate=null, revisedDateStr=null, acceptedDate=1766851200000, acceptedDateStr=2025-12-28, onlineDate=1782451032751, onlineDateStr=2026-06-26, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782451032751, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782451032751, creator=13701087609, updateTime=1782451032751, updator=13701087609, issue=Issue{id=1277255461552390652, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='3', pageStart='553', pageEnd='823', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782451032293, creator='13701087609', updateTime=1782451557265, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257664249852732, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257664249852733, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=579, endPage=587, ext={EN=ArticleExt(id=1277255465327267907, articleId=1277255463477579841, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Protein Interaction Verification of Cassava MeGBSSI and MeSSV, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Granule-bound starch synthase I (MeGBSSI) is the key enzyme for amylose synthesis in cassava, primarily responsible for catalyzing the formation of amylose through α-1,4-glucosidic linkages on the surface of starch granules. However, how MeGBSSI cooperates with other starch-synthetic enzymes to orchestrate starch biosynthesis remains unclear. In the previous stage of this research group, through yeast two-hybrid technology, it was hypothesized that the soluble starch synthase MeSSV was a potential binding protein of MeGBSSI. In this study, the MeGBSSI and MeSSV genes were cloned using the main cultivated variety of cassava, Huanshan 8 (SC8), as the material. Yeast two-hybrid vectors pGBKT7-MeGBSSI and pGADT7-MeSSV were constructed. Through yeast two-hybrid point-to-point experiments, the interaction relationship between MeGBSSI and MeSSV was preliminarily verified. The luciferase complementation (LCA) and double-molecule fluorescence complementation (BiFC) techniques were used to further clarify the direct protein interaction between the two. The results showed that the protein sequences encoded by the MeGBSSI and MeSSV genes, compared with the sequenced variety AM560, respectively, had 4 and 2 amino acid substitutions; the MeGBSSI protein was non-toxic to yeast cells and had no self-activation activity; this protein interacted with the soluble starch synthase MeSSV in yeast; the LCA experiment showed that the chemical signals significantly enhanced after co-expression of MeGBSSI and MeSSV; the BiFC experiment further proved that the two could form a complex in plant cells, and the interaction signal was located in the chloroplast. The findings would provide new insight into the synergistic regulation among cassava starch-synthetic enzymes.

, authors=null, authorsList=Junwen ZHENG, Xiaohua LU, Yuheng LI, Baochang NONG, Yujian GE, Yajie WANG, Yuan YAO, Mengting GENG, authorCompany=null, correspAuthors=Mengting GENG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277255466002550853, articleId=1277255463477579841, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=木薯MeGBSSI与MeSSV的蛋白互作验证, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

颗粒结合淀粉合成酶I（MeGBSSI）是木薯直链淀粉合成的关键酶，主要催化葡萄糖单元在淀粉颗粒上通过α-1,4-糖苷键形成直链淀粉。然而，MeGBSSI与其他淀粉合成相关酶如何协同调控淀粉生物合成的机制仍不明确。本课题组前期通过酵母双杂交技术推测可溶性淀粉合成酶MeSSV为MeGBSSI的潜在结合蛋白。本研究以木薯主栽品种华南8号（SC8）为材料，克隆MeGBSSI和MeSSV基因，构建酵母双杂交载体pGBKT7-MeGBSSI和pGADT7-MeSSV，通过酵母双杂交点对点试验初步验证MeGBSSI与MeSSV之间的互作关系，并采用萤火虫荧光素酶互补（LCA）和双分子荧光互补（BiFC）技术进一步明确二者的直接蛋白互作。结果显示：MeGBSSI和MeSSV基因编码的蛋白序列与测序品种AM560相比，分别发生了4个和2个氨基酸替换；MeGBSSI蛋白对酵母细胞无毒性，且无自激活活性；该蛋白与可溶性淀粉合成酶MeSSV在酵母中发生互作；LCA试验结果显示MeGBSSI与MeSSV共表达后化学信号显著增强；BiFC试验进一步证明二者在植物细胞中能够形成复合体，且互作信号定位于叶绿体。本研究结果为揭示木薯淀粉合成相关酶之间的协同调控机制提供新的视角。

, authors=

郑钧文（1998—），男，硕士研究生，研究方向：作物分子育种。

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* 耿梦婷（GENG Mengting），E-mail: 。
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郑钧文（1998—），男，硕士研究生，研究方向：作物分子育种。

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郑钧文（1998—），男，硕士研究生，研究方向：作物分子育种。

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木薯MeGBSSI与MeSSV的蛋白互作验证
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郑钧文 1, 2 , 陆小花 2 , 李雨恒 1, 2 , 农宝昌 1, 2 , 葛玉建 1, 2 , 王亚杰 2 , 姚远 2 , 耿梦婷 1, *, *
热带作物学报 | 组学与生物技术 2026,47(3): 579-587
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热带作物学报 |组学与生物技术 2026 , 47 (3) : 579 -587
木薯MeGBSSI与MeSSV的蛋白互作验证
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郑钧文1, 2, 陆小花2, 李雨恒1, 2, 农宝昌1, 2, 葛玉建1, 2, 王亚杰2, 姚远2, 耿梦婷1, *, *
作者信息
  • 1．海南大学热带农林学院/热带作物生物育种全国重点实验室，海南海口　570228
  • 2．中国热带农业科学院热带生物技术研究所/中国热带农业科学院三亚研究院/农业农村部热带作物生物学与遗传资源利用重点实验室，海南三亚　572000
通讯作者:
* 耿梦婷（GENG Mengting），E-mail: 。
Protein Interaction Verification of Cassava MeGBSSI and MeSSV
Junwen ZHENG1, 2, Xiaohua LU2, Yuheng LI1, 2, Baochang NONG1, 2, Yujian GE1, 2, Yajie WANG2, Yuan YAO2, Mengting GENG1
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University/National Key Laboratory of Tropical Crop Biotechnology, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources Utilization, Ministry of Agriculture and Rural Affairs, Sanya, Hainan 572000, China
出版时间: 2026-03-25 doi: 10.3969/j.issn.1000-2561.2026.03.003
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摘要
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颗粒结合淀粉合成酶I（MeGBSSI）是木薯直链淀粉合成的关键酶，主要催化葡萄糖单元在淀粉颗粒上通过α-1,4-糖苷键形成直链淀粉。然而，MeGBSSI与其他淀粉合成相关酶如何协同调控淀粉生物合成的机制仍不明确。本课题组前期通过酵母双杂交技术推测可溶性淀粉合成酶MeSSV为MeGBSSI的潜在结合蛋白。本研究以木薯主栽品种华南8号（SC8）为材料，克隆MeGBSSI和MeSSV基因，构建酵母双杂交载体pGBKT7-MeGBSSI和pGADT7-MeSSV，通过酵母双杂交点对点试验初步验证MeGBSSI与MeSSV之间的互作关系，并采用萤火虫荧光素酶互补（LCA）和双分子荧光互补（BiFC）技术进一步明确二者的直接蛋白互作。结果显示：MeGBSSI和MeSSV基因编码的蛋白序列与测序品种AM560相比，分别发生了4个和2个氨基酸替换；MeGBSSI蛋白对酵母细胞无毒性，且无自激活活性；该蛋白与可溶性淀粉合成酶MeSSV在酵母中发生互作；LCA试验结果显示MeGBSSI与MeSSV共表达后化学信号显著增强；BiFC试验进一步证明二者在植物细胞中能够形成复合体，且互作信号定位于叶绿体。本研究结果为揭示木薯淀粉合成相关酶之间的协同调控机制提供新的视角。

木薯  /  MeGBSSI  /  MeSSV  /  酵母双杂交

Granule-bound starch synthase I (MeGBSSI) is the key enzyme for amylose synthesis in cassava, primarily responsible for catalyzing the formation of amylose through α-1,4-glucosidic linkages on the surface of starch granules. However, how MeGBSSI cooperates with other starch-synthetic enzymes to orchestrate starch biosynthesis remains unclear. In the previous stage of this research group, through yeast two-hybrid technology, it was hypothesized that the soluble starch synthase MeSSV was a potential binding protein of MeGBSSI. In this study, the MeGBSSI and MeSSV genes were cloned using the main cultivated variety of cassava, Huanshan 8 (SC8), as the material. Yeast two-hybrid vectors pGBKT7-MeGBSSI and pGADT7-MeSSV were constructed. Through yeast two-hybrid point-to-point experiments, the interaction relationship between MeGBSSI and MeSSV was preliminarily verified. The luciferase complementation (LCA) and double-molecule fluorescence complementation (BiFC) techniques were used to further clarify the direct protein interaction between the two. The results showed that the protein sequences encoded by the MeGBSSI and MeSSV genes, compared with the sequenced variety AM560, respectively, had 4 and 2 amino acid substitutions; the MeGBSSI protein was non-toxic to yeast cells and had no self-activation activity; this protein interacted with the soluble starch synthase MeSSV in yeast; the LCA experiment showed that the chemical signals significantly enhanced after co-expression of MeGBSSI and MeSSV; the BiFC experiment further proved that the two could form a complex in plant cells, and the interaction signal was located in the chloroplast. The findings would provide new insight into the synergistic regulation among cassava starch-synthetic enzymes.

cassava  /  MeGBSSI  /  MeSSV  /  yeast two-hybrid system
郑钧文, 陆小花, 李雨恒, 农宝昌, 葛玉建, 王亚杰, 姚远, 耿梦婷. 木薯MeGBSSI与MeSSV的蛋白互作验证. 热带作物学报, 2026 , 47 (3) : 579 -587 . DOI: 10.3969/j.issn.1000-2561.2026.03.003
Junwen ZHENG, Xiaohua LU, Yuheng LI, Baochang NONG, Yujian GE, Yajie WANG, Yuan YAO, Mengting GENG. Protein Interaction Verification of Cassava MeGBSSI and MeSSV[J]. Chinese Journal of Tropical Crops, 2026 , 47 (3) : 579 -587 . DOI: 10.3969/j.issn.1000-2561.2026.03.003
基金
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  • 海南省自然科学基金项目(325RC816)
  • 中央级公益性科研院所基本科研业务费专项(1630052025020)
  • 国家自然科学基金项目(32460519)
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doi: 10.3969/j.issn.1000-2561.2026.03.003
  • 接收时间：2025-11-18
  • 首发时间：2026-06-26
  • 出版时间：2026-03-25
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  • 收稿日期：2025-11-18
  • 录用日期：2025-12-28
基金
海南省自然科学基金项目(325RC816)
中央级公益性科研院所基本科研业务费专项(1630052025020)
国家自然科学基金项目(32460519)
作者信息
    1．海南大学热带农林学院/热带作物生物育种全国重点实验室，海南海口　570228
    2．中国热带农业科学院热带生物技术研究所/中国热带农业科学院三亚研究院/农业农村部热带作物生物学与遗传资源利用重点实验室，海南三亚　572000

通讯作者:

* 耿梦婷（GENG Mengting），E-mail: 。
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2026.03.003
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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