Article(id=1277240349793841678, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277239982603502113, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2025.05.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1730304000000, receivedDateStr=2024-10-31, revisedDate=null, revisedDateStr=null, acceptedDate=1736179200000, acceptedDateStr=2025-01-07, onlineDate=1782447429369, onlineDateStr=2026-06-26, pubDate=1748102400000, pubDateStr=2025-05-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782447429369, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782447429369, creator=13701087609, updateTime=1782447429369, updator=13701087609, issue=Issue{id=1277239982603502113, tenantId=1146029695717560320, journalId=1235980609244409860, year='2025', volume='46', issue='5', pageStart='1025', pageEnd='1277', issueExtLink='null', onlineDate='null', pubDate='1748102400000', pubDateStr='2025-05-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782447341824, creator='13701087609', updateTime=1782447947315, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277242522292319215, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277239982603502113, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277242522292319216, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277239982603502113, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1043, endPage=1051, ext={EN=ArticleExt(id=1277240358966784527, articleId=1277240349793841678, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Comparative Analysis of Chloroplast Genome Sequences of Five Coffee Species and Its Related Species, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=
Taking the chloroplast genomes of five coffea plants, namely Coffea liberica, C. canephora, C. arabica, C. eugenioides, and C. racemosa, as the research objects, a series of statistical and comparative analyses were carried out. The results demonstrated that the lengths of the chloroplast genomes varied from 154 751 bp to 155 189 bp, with C. arabica a possessing the longest genome and C. canephora having the shortest. The average GC content was determined to be 34.98%. Both C. liberica and C. arabica encoded 130 genes, while C. eugenioides had the largest number of genes, totaling 138, and C. racemosa had the least, with only 121. Upon comparing the border regions of the chloroplast genomes of the five coffee plants, it was observed that the directional sequence arrangements were consistent, thereby reflecting the high conservation of the chloroplast genome structure. The analysis of SSR sequences indicated that the five coffee plants exhibited a pronounced AT bias, and the types and quantities of SSRs differed among them. Through the analysis of nucleotide diversity variation sites, five segments with relatively significant nucleotide variations were identified, namely PetN-PsbM, accD, accD-PsaI, rpl16 and ndhF-rpl32. Furthermore, based on the combined analysis of the chloroplast genomes and the five highly variable nucleotide regions, a phylogenetic tree of 19 Coffea plants was successfully constructed, which shed light on their genetic relationships. This study can serve as a valuable reference for the identification of germplasm resources, the exploration of origin and evolution, as well as the hybrid breeding of Coffea plants.
, authors=null, authorsList=Cheng LIU, Guanrun MA, Mingzhu ZHAO, Meifang LI, Tianming CHEN, Jingmin WANG, Xinlei JIANG, Xuehui BAI, Hua ZHOU, authorCompany=null, correspAuthors=Hua ZHOU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277240428600619548, articleId=1277240349793841678, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=5种咖啡及其近缘种叶绿体基因组序列比较分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=
本研究以大粒种咖啡(Coffea liberica)、中粒种咖啡(C. canephora)、小粒种咖啡(C. arabica)、丁香咖啡(C. eugenioides)及总状咖啡(C. racemosa)5种咖啡属植物的叶绿体基因组为研究对象,对其进行统计和比较分析。结果表明:叶绿体基因组长度在154 751~155 189 bp之间,最长的是小粒种咖啡,最短的是中粒种咖啡,GC平均含量为34.98%;大粒种咖啡和小粒种咖啡叶绿体基因组均编码130个基因,丁香咖啡叶绿体基因组编码基因最多,为138个,总状咖啡叶绿体基因组编码基因最少,为121个;比较5种咖啡植物叶绿体基因组边界区域发现,方向顺序排列一致,体现了叶绿体基因组结构的高度保守性;SSR序列分析表明,5种咖啡植物具有较强的AT偏向性,且SSR类型和数目均不相同;经核苷酸多样性变异位点分析,筛选出了5个核苷酸变异较大的区段,包括PetN-PsbM、accD、accD-PsaI、rpl16和ndhF-rpl32。进一步基于叶绿体基因组和5个核酸高变区的联合分析,构建19种咖啡属植物的系统发育树,揭示其亲缘关系。本研究可为咖啡属植物的种质资源鉴定、起源进化及杂交育种提供参考依据。
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刘成(1994—),男,硕士,助理研究员,研究方向:咖啡遗传育种。
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刘成(1994—),男,硕士,助理研究员,研究方向:咖啡遗传育种。
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刘成(1994—),男,硕士,助理研究员,研究方向:咖啡遗传育种。
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Chloroplast genome boundary analysisJLA: The connection point between LSC and IRa, namely junction of LSC/IRa; JLB: The connection point between LSC and IRb, namely junction of LSC/IRb; JSB: The connection point between SSC and IRb, namely junction of SSC/IRb; JSA: The connection point between SSC and IRa, namely junction of SSC/IRa.
, figureFileSmall=QI0W3Sr0dfuqYyJm0Jzonw==, figureFileBig=NNHluelYEvsh/bpz/TTGDw==, tableContent=null), ArticleFig(id=1277240541809078872, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=图1, caption=
叶绿体基因组边界分析JLA:LSC与IR a的连接点,即junction of LSC/IR a;JLB:LSC与IR b的连接点,即junction of LSC/IR b;JSB:SSC与IR b的连接点,即junction of SSC/IR b;JSA:SSC与IR a的连接点,即junction of SSC/IR a。
, figureFileSmall=QI0W3Sr0dfuqYyJm0Jzonw==, figureFileBig=NNHluelYEvsh/bpz/TTGDw==, tableContent=null), ArticleFig(id=1277240550227047002, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Fig. 2, caption=
Types and numbers of SSRs in chloroplast genomes of five Coffea plants, figureFileSmall=xHVhM1TwbsNW6EFJLR0CMg==, figureFileBig=ZhFq3/fSR4eoTUpqIRwDfA==, tableContent=null), ArticleFig(id=1277240552001237596, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=图2, caption=
5种咖啡属植物叶绿体基因组中SSR类型和数目, figureFileSmall=xHVhM1TwbsNW6EFJLR0CMg==, figureFileBig=ZhFq3/fSR4eoTUpqIRwDfA==, tableContent=null), ArticleFig(id=1277240553611850333, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Fig. 3, caption=
Nucleotide diversity analysis of 19 species of Coffea, figureFileSmall=d22FxxhFJmM54wXYUBw8aQ==, figureFileBig=nkUgM0MXe1mEQIfMSaCpOA==, tableContent=null), ArticleFig(id=1277240556044546654, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=图3, caption=
咖啡属19个物种的核苷酸多样性分析, figureFileSmall=d22FxxhFJmM54wXYUBw8aQ==, figureFileBig=nkUgM0MXe1mEQIfMSaCpOA==, tableContent=null), ArticleFig(id=1277240557718073951, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Fig. 4, caption=
Phylogenetic tree constructed based on complete chloroplast genome, figureFileSmall=vb9NUeOyufKOzFfDcUZMYw==, figureFileBig=zifIp/0mzf+B3aLTTLBFig==, tableContent=null), ArticleFig(id=1277240560670863968, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=图4, caption=
基于叶绿体全基因组构建的系统发育树, figureFileSmall=vb9NUeOyufKOzFfDcUZMYw==, figureFileBig=zifIp/0mzf+B3aLTTLBFig==, tableContent=null), ArticleFig(id=1277240565364290145, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Fig. 5, caption=
Phylogenetic tree constructed based on hypervariable region sequences, figureFileSmall=U123JxOGHzFlDIYyAE09gQ==, figureFileBig=HlfUg+N6/3/XSKl/WvCuWA==, tableContent=null), ArticleFig(id=1277240567079760482, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=图5, caption=
基于高变区序列构建的系统发育树, figureFileSmall=U123JxOGHzFlDIYyAE09gQ==, figureFileBig=HlfUg+N6/3/XSKl/WvCuWA==, tableContent=null), ArticleFig(id=1277240568690373219, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Tab. 1, caption=
Chloroplast genome for hypervariable region and phylogenetic analysis
, figureFileSmall=null, figureFileBig=null, tableContent=
| 物种Species | GenBank登录号GenBank accession number | 备注Note |
|---|
丁香咖啡 C. eugenioides | NC083907.1 | 咖啡属 |
大粒种咖啡 C. liberica | MW970411.1 | |
中粒种咖啡 C. canephora | KU500324.1 | |
小粒种咖啡 C. arabica | EF044213.1 | |
总状咖啡 C. racemosa | MK577911.1 | |
小粒种咖啡红波邦 C. arabica cultivar Red Bourbon | CM061598.1 | |
曼尼咖啡 Coffea mannii | MK577918.1 | |
霍斯菲尔德咖啡 C. horsfieldiana | MK577917.1 | |
无苞片咖啡 C. ebracteolata | MK577916.1 | |
米什米咖啡 C. benghalensis | MK577915.1 | |
米什米咖啡巴巴杜安咖啡 C. benghalensis var. Bababudanii | MK577914.1 | |
四角咖啡 C. tetragona | MK577913.1 | |
狭叶咖啡 C. stenophylla | MK577912.1 | |
假桑给巴尔咖啡 C. pseudozanguebariae | MK577910.1 | |
大果咖啡 C. macrocarpa | MK577909.1 | |
矮咖啡 C. humilis | MK577908.1 | |
昂布洛特咖啡 C. humblotiana | MK577907.1 | |
长叶咖啡 C. dolichophylla | MK577906.1 | |
布拉斯咖啡 C. brassii | MK577905.1 | |
栀子 Gardenia jasminoides | MW160432.1 | 外内群 |
), ArticleFig(id=1277240571236315748, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=表1, caption=
用于高变区及系统发育分析的叶绿体基因组
, figureFileSmall=null, figureFileBig=null, tableContent=
| 物种Species | GenBank登录号GenBank accession number | 备注Note |
|---|
丁香咖啡 C. eugenioides | NC083907.1 | 咖啡属 |
大粒种咖啡 C. liberica | MW970411.1 | |
中粒种咖啡 C. canephora | KU500324.1 | |
小粒种咖啡 C. arabica | EF044213.1 | |
总状咖啡 C. racemosa | MK577911.1 | |
小粒种咖啡红波邦 C. arabica cultivar Red Bourbon | CM061598.1 | |
曼尼咖啡 Coffea mannii | MK577918.1 | |
霍斯菲尔德咖啡 C. horsfieldiana | MK577917.1 | |
无苞片咖啡 C. ebracteolata | MK577916.1 | |
米什米咖啡 C. benghalensis | MK577915.1 | |
米什米咖啡巴巴杜安咖啡 C. benghalensis var. Bababudanii | MK577914.1 | |
四角咖啡 C. tetragona | MK577913.1 | |
狭叶咖啡 C. stenophylla | MK577912.1 | |
假桑给巴尔咖啡 C. pseudozanguebariae | MK577910.1 | |
大果咖啡 C. macrocarpa | MK577909.1 | |
矮咖啡 C. humilis | MK577908.1 | |
昂布洛特咖啡 C. humblotiana | MK577907.1 | |
长叶咖啡 C. dolichophylla | MK577906.1 | |
布拉斯咖啡 C. brassii | MK577905.1 | |
栀子 Gardenia jasminoides | MW160432.1 | 外内群 |
), ArticleFig(id=1277240573257970277, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=EN, label=Tab. 2, caption=
Chloroplast genomes of five Coffea species
, figureFileSmall=null, figureFileBig=null, tableContent=
| 物种Species | 基因组长度Genome lengtd/bp | LSC长度LSC lengtd/bp | SSC长度SSC lengtd/bp | IR长度IR lengtd/bp | 总基因数Total number of genes | 蛋白编码基因Protein-coding genes |
| 丁香咖啡 | 155 123 | 85 159 | 18 136 | 25 914 | 138 | 85 |
| 总状咖啡 | 154 942 | 85 128 | 18 114 | 25 850 | 121 | 85 |
| 大粒种咖啡 | 154 799 | 84 868 | 18 121 | 25 905 | 130 | 85 |
| 中粒种咖啡 | 154 751 | 86 474 | 53 243 | 7517 | 131 | 86 |
| 小粒种咖啡 | 155 189 | 85 166 | 18 137 | 25 943 | 130 | 85 |
| 物种Species | rRNA基因数Number of rRNA genes | tRNA基因数Number of tRNA genes | GC含量GC content/% | LSC的GC含量GC content of LSC/% | IR的GC含量GC content of IR/% | SSC的GC含量GC content of SSC/% |
| 丁香咖啡 | 8 | 45 | 37.4 | 35.4 | 43.0 | 31.3 |
| 总状咖啡 | 0 | 36 | 37.4 | 35.3 | 43.1 | 31.4 |
| 大粒种咖啡 | 8 | 37 | 31.3 | 35.4 | 43.1 | 31.3 |
| 中粒种咖啡 | 8 | 37 | 37.5 | 35.5 | 37.9 | 40.6 |
| 小粒种咖啡 | 8 | 37 | 31.3 | 35.3 | 43.0 | 31.3 |
), ArticleFig(id=1277240576198177382, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277240349793841678, language=CN, label=表2, caption=
5种咖啡属植物的叶绿体基因组
, figureFileSmall=null, figureFileBig=null, tableContent=
| 物种Species | 基因组长度Genome lengtd/bp | LSC长度LSC lengtd/bp | SSC长度SSC lengtd/bp | IR长度IR lengtd/bp | 总基因数Total number of genes | 蛋白编码基因Protein-coding genes |
| 丁香咖啡 | 155 123 | 85 159 | 18 136 | 25 914 | 138 | 85 |
| 总状咖啡 | 154 942 | 85 128 | 18 114 | 25 850 | 121 | 85 |
| 大粒种咖啡 | 154 799 | 84 868 | 18 121 | 25 905 | 130 | 85 |
| 中粒种咖啡 | 154 751 | 86 474 | 53 243 | 7517 | 131 | 86 |
| 小粒种咖啡 | 155 189 | 85 166 | 18 137 | 25 943 | 130 | 85 |
| 物种Species | rRNA基因数Number of rRNA genes | tRNA基因数Number of tRNA genes | GC含量GC content/% | LSC的GC含量GC content of LSC/% | IR的GC含量GC content of IR/% | SSC的GC含量GC content of SSC/% |
| 丁香咖啡 | 8 | 45 | 37.4 | 35.4 | 43.0 | 31.3 |
| 总状咖啡 | 0 | 36 | 37.4 | 35.3 | 43.1 | 31.4 |
| 大粒种咖啡 | 8 | 37 | 31.3 | 35.4 | 43.1 | 31.3 |
| 中粒种咖啡 | 8 | 37 | 37.5 | 35.5 | 37.9 | 40.6 |
| 小粒种咖啡 | 8 | 37 | 31.3 | 35.3 | 43.0 | 31.3 |
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