热带作物学报

蚯蚓对新烟碱类杀虫剂吡虫啉、啶虫脒污染红壤中细菌群落结构的影响

PDF下载

王娇娇 ¹^,² , 武春媛 ²^,³ , 谭华东 ²^,³ , 李怡 ²^,³^,^*

热带作物学报 | 植物保护与生物安全 2023,44(9): 1860-1868

收起

热带作物学报 | 植物保护与生物安全 2023, 44(9): 1860-1868

蚯蚓对新烟碱类杀虫剂吡虫啉、啶虫脒污染红壤中细菌群落结构的影响

全屏

王娇娇¹^,², 武春媛²^,³, 谭华东²^,³, 李怡²^,³^,^*

作者信息

1.海南大学生态与环境学院，海南海口 570228

2.中国热带农业科学院环境与植物保护研究所，海南海口 571101

3.农业农村部热区高效农业绿色低碳重点实验室，海南海口 571101

王娇娇（1995—），女，硕士研究生，研究方向：环境微生物。

通讯作者:

^* 李怡（LI Yi），E-mail: liyi@catas.cn。

Effects of Earthworms on Bacterial Community Structure in Red Soil Contaminated by Neonicotinic Insecticides Imidacloprid and Acetamiprid

Jiaojiao WANG¹^,², Chunyuan WU²^,³, Huadong TAN²^,³, Yi LI²^,³^,^*

Affiliations

1. College of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China

2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China

3. Key Laboratory of Green and Low Carbon Green Agriculture in Tropical China, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China

出版时间: 2023-09-25 doi: 10.3969/j.issn.1000-2561.2023.09.014

文章导航

摘要

收起

新烟碱类杀虫剂（neonicotinoids, NEOs）吡虫啉（imidacloprid, IMI）、啶虫脒（acetamiprid, ACE）是目前世界上使用较为广泛的杀虫剂。本研究以赤子爱胜蚓（Eisenia foetida）为土壤动物，采用生物学模拟方法，通过高通量测序、土壤理化性质测定，分析IMI、ACE污染红壤（BCK、DCK）和引入蚯蚓后红壤（B、D）中细菌群落结构变化及其与环境因子之间的关系，探究污染红壤的动物修复机制。结果表明：与自然红壤（CK）相比，加入IMI、ACE降低了红壤的微生物丰富度和多样性，显著改变了土壤原有的细菌群落结构，使土壤有机质（SOM）和全氮（TN）含量下降；而蚯蚓的加入，增加了土壤中具有杀虫剂耐药性和降解杀虫剂能力的放线菌门（Actinobacteriota）、变形菌门（Proteobacteria）和拟杆菌门（Bacteroidota）等的相对丰度，提高了污染土壤pH、TN、全磷（TP）、全钾（TK）含量；结合土壤理化性质的相关性分析可知，在属水平上，pH、TN、TK和SOM对各处理组细菌群落结构的影响较大。研究表明，IMI、ACE污染降低了土壤TN和SOM含量，破坏了土壤中原有的细菌群落结构；蚯蚓活动提高了污染红壤的土壤肥力，缓解了IMI、ACE对土壤中微生物菌群的不利影响，在一定程度上修复和丰富了NEOs污染土壤的微生物群落物种多样性，进而加速了土壤中NEOs的降解。该研究结果为从蚯触圈中筛选出高效IMI、ACE降解菌提供可行性，可为蚯蚓对IMI、ACE污染土壤的修复提供理论基础。

关键词

蚯蚓 / 细菌群落 / 吡虫啉 / 啶虫脒 / 红壤 / 环境因子

Abstract

收起

Neonicotinoids (NEOs) contain imidacloprid (IMI) and acetamiprid (ACE), which are widely used in the world. In this study, earthworms (Eisenia foetida) were used as soil animal. Through high-throughput sequencing and measurement of soil physical and chemical properties, the changes of bacterial communities between IMI and ACE contaminated red soil (BCK, DCK) and soil after earthworm introduction (B, D), and the relationship with environmental factors were analyzed to explore the animal remediation mechanism of contaminated red soil. The introduction of IMI and ACE reduced the diversity of microbial population, significantly changed the composition of soil bacterial community structure, and decreased the content of soil organic matter (SOM) and total nitrogen (TN), compared with the natural red soil (CK). The addition of earthworms increased the relative abundance of Actinobacteriota, Proteobacteria and Bacteroidota, which have the ability of insecticide resistance and degrading. pH value, TN, total phosphorus (TP) and total potassium (TK) contents of contaminated soil increased. Combined with the correlation analysis of soil physiochemical properties, it was found that pH, TN, TK and SOM had a great influence on the bacterial community structure among the treatment groups at the genus level. The study showed that IMI and ACE pollution reduced the contents of SOM and soil TN, and destructed the original bacterial community structure in the soil. Earthworm activity improved soil fertility of contaminated red soil, alleviated the adverse effects of IMI and ACE on soil microbial flora, restored and enriched the diversity of microbial species in NEOs-contaminated soil to a certain extent, and then accelerated the degradation of NEOs in soil. This study showed the feasibility to screen the efficiently IMI and ACE-degrading bacteria from drilosphere, and would provide theoretical basis for the earthworm remediation of IMI and ACE-contaminated soil.

Key words

earthworm / bacterial community / imidacloprid / acetamiprid / red soil / environmental factor

引用本文

王娇娇, 武春媛, 谭华东, 李怡. 蚯蚓对新烟碱类杀虫剂吡虫啉、啶虫脒污染红壤中细菌群落结构的影响. 热带作物学报, 2023 , 44 (9) : 1860 -1868 . DOI: 10.3969/j.issn.1000-2561.2023.09.014

Jiaojiao WANG, Chunyuan WU, Huadong TAN, Yi LI. Effects of Earthworms on Bacterial Community Structure in Red Soil Contaminated by Neonicotinic Insecticides Imidacloprid and Acetamiprid[J]. Chinese Journal of Tropical Crops, 2023 , 44 (9) : 1860 -1868 . DOI: 10.3969/j.issn.1000-2561.2023.09.014

基金

收起

海南省自然科学基金项目(2019RC266)

参考文献

收起

2023年第44卷第9期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.3969/j.issn.1000-2561.2023.09.014

接收时间：2022-10-19
首发时间：2026-03-05
出版时间：2023-09-25

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2022-10-19
修回日期：2023-02-02

基金

海南省自然科学基金项目(2019RC266)

作者信息

1.海南大学生态与环境学院，海南海口 570228

2.中国热带农业科学院环境与植物保护研究所，海南海口 571101

3.农业农村部热区高效农业绿色低碳重点实验室，海南海口 571101

通讯作者:

^* 李怡（LI Yi），E-mail: liyi@catas.cn。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2023.09.014

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT