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It is of great significance to explore the characteristics and functions of GRAS gene family in elephant grass (Pennisetum purpureum) and clarify their regulatory roles under low-temperature stress. Based on the genomic data of elephant grass, in this study we identified members of the GRAS gene family, and analyzed their chromosomal localization, physicochemical properties, gene structural characteristics, phylogenetic relationships, as well as the expression patterns of elephant grass materials with different cold resistance under low-temperature stress. The results showed that a total of 18 GRAS genes were identified in the elephant grass genome, which were distributed on 10 chromosomes and classified into five subfamilies via phylogenetic analysis. Cis-acting element analysis revealed that the promoter regions of 10 PpGRAS members contained regulatory elements related to low-temperature response. Collinearity analysis indicated that elephant grass shared more homologous genes with Sorghum bicolor than that with Oryza sativa. Expression pattern analysis demonstrated that the expression levels of multiple GRAS family members, especially genes such as PpGRAS7 and PpGRAS10 belonging to the LISCL subfamily, were significantly positively correlated with cold resistance capacity of materials. This study provides valuable references for further exploring the functions of elephant grass GRAS family and their response mechanisms to Low-temperature stress., authors=CAO Lei1, YANG Zhong-fu2, FENG Guang-yan1, ZHANG Xin-quan1, authorsList=CAO Lei, YANG Zhong-fu, FENG Guang-yan, ZHANG Xin-quan, authorCompany=1 College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China; 2. College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575131438089086, articleId=1309575131027047293, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=象草GRAS基因家族鉴定及表达分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=GRAS家族是植物体内普遍存在的一类特异性转录调控因子,在调控植物生长发育、应对生物与非生物逆境胁迫等过程中发挥重要作用。探究象草GRAS基因家族基因的特征及功能,解析其在低温胁迫下的调控作用具有重要意义。本研究以象草基因组数据为基础,对GRAS家族成员进行鉴定,并分析染色体位置、理化特性、基因结构特征、系统进化关系等,以及低温胁迫条件下不同抗寒性象草材料的表达模式。结果表明,在象草基因组中鉴定到18个GRAS基因,分布在10条染色体上,系统进化分析将其分为5个亚家族。顺式作用元件分析表明有10个PpGRAS成员的启动子区域含有低温应答相关调控元件。共线性分析表明象草与高粱(Sorghum bicolor)的同源基因数量显著多于水稻(Oryza sativa)。表达模式分析发现GRAS基因家族中多个成员,特别是亚家族LISCL,如基因PpGRAS7、PpGRAS10等的表达模式与材料的抗寒能力呈显著正相关。研究结果为深入探讨象草GRAS家族及其低温胁迫响应提供了有价值的信息。, authors=曹磊1, 杨忠富2, 冯光燕1, 张新全1, authorsList=曹磊, 杨忠富, 冯光燕, 张新全, authorCompany=1 四川农业大学草业科技学院, 四川 成都 611130; 2 贵州大学动物科学学院, 贵州 贵阳 550025, correspAuthors=null, authorNote=曹磊(1999-),男,汉族,山西晋中人,硕士研究生,主要从事草种质资源创新与利用方向研究,E-mail:209361903@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=Yvdf70OeWiTxwwRADssMag==, pdfFileSize=7060363, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, 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Nature Plants, 2017, 3: 17010 LU X, LIU W, XIANG C, et al. Genome-Wide Characterization of GRAS Family and Their Potential Roles in Cold Tolerance of Cucumber (Cucumis sativus L.)[J]. International Journal of Molecular Sciences, 2020, 21(11): 3857 BOLLE C. The role of GRAS proteins in plant signal transduction and development[J]. Planta, 2004, 218(5): 683-692 NIU Y L, JIANG X M, XU X Y, et al. Research progress of plant transcription factor GRAS proteins[J]. Genomics and Applied Biology, 2016, 35(9): 2519-2524 牛义岭,姜秀明,许向阳,等.植物转录因子GRAS蛋白的研究进展[J].基因组学与应用生物学,2016,35(9):2519-2524 SUN X L, JONES W T, RIKKERINK E. GRAS proteins: the versatile roles of intrinsically disordered proteins in plant signalling[J]. BIOCHEM Journal, 2012, 442(1): 1-12 YUDONG L, WEI H, ZHIQIANG X, et al. Overexpression of SlGRAS40 in Tomato Enhances Tolerance to Abiotic Stresses and Influences Auxin and Gibberellin Signaling[J]. Frontiers in Plant Science, 2017, 8: 1659 WANG X, LI G, SUN Y, et al. Genome-wide analysis and characterization of GRAS family in switchgrass [J]. Bioengineered, 2021, 12(1): 6096-6114 LI L L, ZENG W J, LI Y H, et al. Genome-wide analysis of GRAS transcription factor family and cloning and cold response of LaSCL18 gene in Lepidium apetalum [J]. Molecular Plant Breeding, 2017, 15(9): 3428-3437 李丽丽,曾卫军,李艳红,等.独行菜GRAS转录因子家族分析及LaSCL18基因克隆与冷相关性研究[J].分子植物育种,2017,15(9):3428-3437 ACHARD P, GONG F, CHEMINANT S, et al. The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism[J]. The Plant Cell, 2008, 20(8): 2117-2129 YANG T, LI C, ZHANG H, et al. Genome-wide identification and expression analysis of the GRAS transcription in eggplant (Solanum melongena L.)[J]. Frontiers in Genetics, 2022, 13: 932731 YUAN Y, FANG L, KARUNGO S K, et al. Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis[J]. Plant Cell Reports, 2016(35):655-666 TANABE S, ONODERA H, HARA N, et al. The elicitor-responsive gene for a GRAS family protein, CIGR2, suppresses cell death in rice inoculated with rice blast fungus via activation of a heat shock transcription factor, OsHsf23[J]. Biosci Biotechnol Biochem, 2016, 80(1): 145-151 TIAN C, WAN P, SUN S, et al. Genome-Wide Analysis of the GRAS Gene Family in Rice and Arabidopsis[J]. Plant Molecular Biology, 2004, 54(4): 519-532 WANG L, DING X, GAO Y, et al. Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)[J]. BMC Plant Biology, 2020, 20(1): 415 TO V T, SHI Q, ZHANG Y, et al. Genome-Wide Analysis of the GRAS Gene Family in Barley (Hordeum vulgare L.)[J]. Genes, 2020, 11(5): 553 WU Z Q, CHEN W, ZHAO Z, et al. Genome-wide identification and bioinformatics analysis of GRAS gene family in maize (Zea mays L.) [J]. Journal of Agricultural Science and Technology, 2024, 26(3): 15-25 吴占清,陈威,赵展,等.玉米GRAS基因家族的全基因组鉴定及生物信息学分析[J].中国农业科技导报,2024,26(3):15-25 XU X, FENG G, HUANG L, et al. Genome-wide identification, structural analysis and expression profiles of GRAS gene family in orchardgrass[J]. Molecular Biology Reports, 2020, 47(3): 1845-1857 PAN J. Physiological response of oat to salt and drought stress and identification and expression analysis of GRAS gene family[D]. Chengdu: Southwest Minzu University, 2023:55 潘静.燕麦对盐、干旱胁迫的生理响应及其GRAS基因家族鉴定与表达分析[D].成都:西南民族大学,2023:55 LI J, YUAN Y, WU T K, et al. Identification and expression analysis of GRAS gene family in Hevea brasiliensis[J]. Chinese Journal of Tropical Crops, 2024, 45(9): 1761-1779 李娟,袁渊,吴挺开,等.巴西橡胶树GRAS基因家族鉴定及表达分析[J].热带作物学报,2024,45(9):1761-1779 LU H J, HOU X L. Identification of GRAS gene family in non-heading Chinese cabbage and its expression analysis under low temperature stress [J/OL].https://link.cnki.net/urlid/46.1068.S.20240418.1221.010,2024-04-19/2026-06-15 卢宏杰,侯喜林.不结球白菜GRAS基因家族鉴定及其在低温胁迫下的表达分析[J/OL].https://link.cnki.net/urlid/46.1068.S.20240418.1221.010,2024-04-19/2026-06-15 YANG P Y, LIANG H K, YIN C P, et al. Genome-wide identification of GRAS gene family in sorghum and its response to uniconazole[J]. Journal of Agricultural University of Hebei, 2021, 44(5): 1-13 杨溥原,梁红凯,殷丛培,等.高粱GRAS基因家族全基因组鉴定及其对烯效唑的响应[J].河北农业大学学报,2021,44(5):1-13 TANG Z J. Identification and expression analysis of BdGRAS family genes in Brachypodium distachyon[D]. Changsha: Hunan Agricultural University, 2021:16-17 唐泽珺.二穗短柄草BdGRAS家族基因鉴定与表达分析[D].长沙:湖南农业大学,2021:16-17 ZHU T. Identification, phylogenetic evolution and expression analysis of bHLH transcription factor family in barley[D]. Yangling: Northwest A&F University, 2019:39 朱婷.大麦bHLH转录因子家族的鉴定及系统进化和表达分析[D].杨凌:西北农林科技大学,2019:39 韩慧,张秀海,郭建波,等.DELLA蛋白在植物中的功能[J/OL].https://link.cnki.net/urlid/46.1068.S.20241216.1105.002,2024-12-26/2026-06-15)
Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum
CAO Lei1, YANG Zhong-fu2, FENG Guang-yan1, ZHANG Xin-quan1
Affiliations
1 College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China; 2. College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, China
The GRAS family is a class of specific transcriptional regulatory factors widely present in plants, which play vital roles in regulating plant growth and development, as well as responses to biotic and abiotic stress. It is of great significance to explore the characteristics and functions of GRAS gene family in elephant grass (Pennisetum purpureum) and clarify their regulatory roles under low-temperature stress. Based on the genomic data of elephant grass, in this study we identified members of the GRAS gene family, and analyzed their chromosomal localization, physicochemical properties, gene structural characteristics, phylogenetic relationships, as well as the expression patterns of elephant grass materials with different cold resistance under low-temperature stress. The results showed that a total of 18 GRAS genes were identified in the elephant grass genome, which were distributed on 10 chromosomes and classified into five subfamilies via phylogenetic analysis. Cis-acting element analysis revealed that the promoter regions of 10 PpGRAS members contained regulatory elements related to low-temperature response. Collinearity analysis indicated that elephant grass shared more homologous genes with Sorghum bicolor than that with Oryza sativa. Expression pattern analysis demonstrated that the expression levels of multiple GRAS family members, especially genes such as PpGRAS7 and PpGRAS10 belonging to the LISCL subfamily, were significantly positively correlated with cold resistance capacity of materials. This study provides valuable references for further exploring the functions of elephant grass GRAS family and their response mechanisms to Low-temperature stress.
Key words
Pennisetum purpureum
/
GRAS gene family
/
low-temperature stress
CAO Lei, YANG Zhong-fu, FENG Guang-yan, ZHANG Xin-quan.
Identification and Expression Analysis of GRAS Gene Family in Pennisetum purpureum[J].
Acta Agrestia Sinica,
2026
, 34
(9)
: 3172
-3184
.
DOI: 10.11733/j.issn.1007-0435.2026.09.004
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Genome-wide analysis and characterization of GRAS family in switchgrass [J]. Bioengineered, 2021, 12(1): 6096-6114 LI L L, ZENG W J, LI Y H, et al. Genome-wide analysis of GRAS transcription factor family and cloning and cold response of LaSCL18 gene in Lepidium apetalum [J]. Molecular Plant Breeding, 2017, 15(9): 3428-3437 李丽丽,曾卫军,李艳红,等.独行菜GRAS转录因子家族分析及LaSCL18基因克隆与冷相关性研究[J].分子植物育种,2017,15(9):3428-3437 ACHARD P, GONG F, CHEMINANT S, et al. The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism[J]. The Plant Cell, 2008, 20(8): 2117-2129 YANG T, LI C, ZHANG H, et al. Genome-wide identification and expression analysis of the GRAS transcription in eggplant (Solanum melongena L.)[J]. Frontiers in Genetics, 2022, 13: 932731 YUAN Y, FANG L, KARUNGO S K, et al. Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis[J]. Plant Cell Reports, 2016(35):655-666 TANABE S, ONODERA H, HARA N, et al. The elicitor-responsive gene for a GRAS family protein, CIGR2, suppresses cell death in rice inoculated with rice blast fungus via activation of a heat shock transcription factor, OsHsf23[J]. Biosci Biotechnol Biochem, 2016, 80(1): 145-151 TIAN C, WAN P, SUN S, et al. Genome-Wide Analysis of the GRAS Gene Family in Rice and Arabidopsis[J]. Plant Molecular Biology, 2004, 54(4): 519-532 WANG L, DING X, GAO Y, et al. Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)[J]. BMC Plant Biology, 2020, 20(1): 415 TO V T, SHI Q, ZHANG Y, et al. Genome-Wide Analysis of the GRAS Gene Family in Barley (Hordeum vulgare L.)[J]. Genes, 2020, 11(5): 553 WU Z Q, CHEN W, ZHAO Z, et al. Genome-wide identification and bioinformatics analysis of GRAS gene family in maize (Zea mays L.) [J]. Journal of Agricultural Science and Technology, 2024, 26(3): 15-25 吴占清,陈威,赵展,等.玉米GRAS基因家族的全基因组鉴定及生物信息学分析[J].中国农业科技导报,2024,26(3):15-25 XU X, FENG G, HUANG L, et al. Genome-wide identification, structural analysis and expression profiles of GRAS gene family in orchardgrass[J]. Molecular Biology Reports, 2020, 47(3): 1845-1857 PAN J. Physiological response of oat to salt and drought stress and identification and expression analysis of GRAS gene family[D]. Chengdu: Southwest Minzu University, 2023:55 潘静.燕麦对盐、干旱胁迫的生理响应及其GRAS基因家族鉴定与表达分析[D].成都:西南民族大学,2023:55 LI J, YUAN Y, WU T K, et al. Identification and expression analysis of GRAS gene family in Hevea brasiliensis[J]. Chinese Journal of Tropical Crops, 2024, 45(9): 1761-1779 李娟,袁渊,吴挺开,等.巴西橡胶树GRAS基因家族鉴定及表达分析[J].热带作物学报,2024,45(9):1761-1779 LU H J, HOU X L. 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Yangling: Northwest A&F University, 2019:39 朱婷.大麦bHLH转录因子家族的鉴定及系统进化和表达分析[D].杨凌:西北农林科技大学,2019:39 韩慧,张秀海,郭建波,等.DELLA蛋白在植物中的功能[J/OL].https://link.cnki.net/urlid/46.1068.S.20241216.1105.002,2024-12-26/2026-06-15