Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions
YI Guo-yun1,2, CHENG Liang1,2, LI Juan1,2, WANG Xin1
Affiliations
1 Academy of Agriculture and Forestry, Qinghai University, Xining, Qinghai Province 810016, China; 2 Qinghai Provincial Key Laboratory of Agricultural Integrated Pest Management, Xining, Qinghai Province 810016, China
To screen plant growth-promoting rhizobacteria （PGPR） that are capable of stably colonizing the rhizosphere of Stipa purpurea， four PGPR strains previously isolated from this alpine grass were evaluated. Rifampicin-resistant derivatives were generated to monitor root-zone colonization dynamics， and the effects of bacterial inoculation on seed germination， seedling morphology， and physiological indices were assessed under the culture of 15℃， 20℃， and 25℃. Serratia proteamaculans GD-1-1 and Paenarthrobacter nitroguajacolicus YD-2-4 established stable populations in the S. purpurea rhizosphere， and the colonization amounts were 1.22×108 and 0.06×108 CFU·g-1 at 20 d， respectively. Dilutions of YD-2-4 （1×101 and 1×102 CFU·mL-1） significantly enhanced germination and seedling growth across 15℃-25℃， concurrently increasing antioxidant enzyme activities and lowering malondialdehyde content. In contrast， the stimulatory effect of GD-1-1 （1×102 and 1×103 CFU·mL-1） declined with temperature and disappeared at 15℃. These results indicated that YD-2-4 possessed robust colonization ability and broad temperature adaptability， making it an excellent candidate for the development of microbial inoculants for alpine grasslands.
YI Guo-yun, CHENG Liang, LI Juan, WANG Xin.
Colonization of Plant Growth-Promoting Rhizobacteria and its Effects on the Growth of Stipa Purpurea Under Different Temperature Conditions[J].
Acta Agrestia Sinica,
2026
, 34
(6)
: 2316
-2328
.
DOI: 10.11733/j.issn.1007-0435.2026.06.034
基金
收起
青海省自然科学基金项目项目-青年 (2024-ZJ-942)
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DONG S K，TANG L，ZHANG X F，et al. Relationship between plant species diversity and functional diversity in alpine grassland[J]. Acta Ecologica Sinica，2017（5）：1472-1483 董世魁，汤琳，张相锋，等. 高寒草地植物物种多样性与功能多样性的关系[J]. 生态学报，2017（5）：1472-1483 ZHANG J T. Exploring the influence of vegetation characteristics and environmental factors in typical alpine degraded grasslands on the Qing-Zang plateau[D]. Linfen：Shanxi Normal University，2022：2-7 张锦涛. 青藏高原典型高寒退化草地植被特征与环境因子的影响探究[D]. 临汾：山西师范大学，2022：2-7 LI M，WANG J B，ZHANG X J，et al. Distribution of potential suitable areas of dominant species in alpine grasslands and alpine meadows in the TP under future climate scenarios[J]. Acta Ecologica Sinica，2024，44（22）：10162-10177 李明，王军邦，张秀娟，等. 未来气候情景下青藏高原高寒草原与高寒草甸优势种潜在适宜区分布预测[J]. 生态学报，2024，44（22）：10162-10177 LU D X， JIN H，YANG X Y，et al. Characterization of rhizosphere and endophytic fungal communities from roots of Stipa purpurea in alpine steppe around Qinghai Lake[J]. Canadian Journal of Microbiology，2016，62（8）：643-656 HAROON U，MUNIS M F H，LIAQUAT F，et al. Biofilm formation and flocculation potential analysis of halotolerant Bacillus tequilensis and its inoculation in soil to mitigate salinity stress of chickpea[J]. Physiology and Molecular Biology of Plants，2023，29（2）：277-288 QI Z Q，LI X H，WANG Y Z，et al. Colonizing of antagonistic bacterium B67 isolate on soil and rhizosphere of cucumber[J]. China Vegetables，2009（8）：63-66 祁之秋，李兴海，王英姿，等. 黄瓜枯萎病拮抗芽孢杆菌B67在土壤及黄瓜根际的定殖[J]. 中国蔬菜，2009（8）：63-66 JIN Y，GAO J. Study on colonization of endophytic antagonistic strain JYg07 of Schisandra chinensis[J]. Chinese Traditional and Herbal Drugs，2015，46（21）：3242-3247 金岩，高洁. 五味子内生拮抗菌JYg07的定殖研究[J]. 中草药，2015，46（21）：3242-3247 YANG X X，HUANG X Q，ZHANG L，et al. Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus[J]. Chinese Agricultural Science Bulletin，2022，38（1）：125-130 杨潇湘，黄小琴，张蕾，等. 绿色荧光蛋白标记解淀粉芽孢杆菌Bam22在油菜体内的定殖[J]. 中国农学通报，2022，38（1）：125-130 WANG Z G，LIU Z P，HU Y L，et al. Molecular marker and colonization of Plantibacter sp. WZW03[J]. Acta Agriculturae Zhejiangensis，2018，30（10）：1715-1721 王志刚，刘泽平，胡云龙，等. 植物杆菌属（Plantibacter）菌株WZW03的分子标记与定殖促生能力[J]. 浙江农业学报，2018，30（10）：1715-1721 LI Y，LONG C M，JIANG B，et al. Colonization on the peanuts of two plant-growth promoting rhizobacteria strains and effects on the bacterial community structure of rhizosphere[J]. Biotechnology Bulletin，2022，38（9）：237-247 李颖，龙长梅，蒋标，等. 两株PGPR菌株的花生定殖及对根际细菌群落结构的影响[J]. 生物技术通报，2022，38（9）：237-247 SONG L Y，GAO P，ZHANG H，et al. Isolation，identification and colonization of biocontrol bacteria ZB36 against bitter gourd Fusarium wilt[J]. Journal of Henan Agricultural University，2024，58（2）：228-238 宋露洋，高沛，张涵，等. 苦瓜枯萎病生防细菌ZB36的分离鉴定及其定殖特性研究[J]. 河南农业大学学报，2024，58（2）：228-238 WANG J Q，WANG J J，WANG Q，et al. Colonization of double-resistance strain of marine bacterium L1-9 and its biocontrol effect on Fusarium wilt of cucumber[J]. Biotechnology Bulletin，2016，32（6）：193-198 王军强，汪晶晶，王琦，等. 海洋细菌L1-9双抗菌株的定殖能力及其对黄瓜枯萎病的防治作用[J]. 生物技术通报，2016，32（6）：193-198 LI M Y. Characterization and plant growth-promoting mechanism of cold-adapted PGPB from dominant herbage in the alpine grassland of the Qilian mountains[D]. Lanzhou：Gansu Agricultural University，2022：12-30 李明源. 祁连山高寒草地优势牧草耐冷促生菌特性及促生机理研究[D]. 兰州：甘肃农业大学，2022：12-30 LEI S Y，ZHANG Y，SU B B，et al. Effects of plant growth promoting rhizobacteria （PGPR） on three cultivated forages in alpine area[J]. Acta Agrestia Sinica，2025，33（7）：2388-2399 雷生妍，张英，苏贝贝，等. 植物根际促生菌（PGPR）对高寒区3种栽培饲草促生效果研究[J]. 草地学报，2025，33（7）：2388-2399 AO Y，FENG J R，YANG C D. Function determination and identification of endophytic bacteria 263ZY2 from Stipa purpurea in Alpine Grassland[J]. Grassland and Turf，2023，43（6）：38-43 敖远，冯疆蓉，杨成德. 高寒草地紫花针茅拮抗内生细菌263ZY2的功能测定及其鉴定[J]. 草原与草坪，2023，43（6）：38-43 YANG C D，CUI Y Z，FENG Z H，et al. Effects of endophytic Bacillus subtilis 265ZY4 on physiological and biochemical characteristics of Stipa purpurea under abiotic stress[J]. Acta Prataculturae Sinica，2019，28（6）：101-108 杨成德，崔月贞，冯中红，等. 内生枯草芽孢杆菌265ZY4对温度和紫外光胁迫下紫花针茅生化特征的影响[J]. 草业学报，2019，28（6）：101-108 GÓMEZ E，ALONSO A，SÁNCHEZ J，et al. Application of biostimulant in seeds and soil on three chickpea varieties： impacts on germination，vegetative development，and bacterial facilitation of nitrogen and phosphorus[J]. Life，2024，14（1）：148 SONG J Q，YIN Y L，ZHAO W，et al. Characteristics of spatial differentiation of soil microbial communities in degraded grassland on the “black soil beaches” of Qinghai Plateau[J]. Ecology and Environmental Sciences，2024，33（11）：1696-1707 宋江琴，尹亚丽，赵文，等. 青海高原黑土滩退化草地土壤微生物群落空间分异特征[J]. 生态环境学报，2024，33（11）：1696-1707 YI G Y，LI J，WANG X，et al. Screening of plant growth-promoting rhizobacteria and their growth-promoting activities for Stipa purpurea in alpine grassland[J]. Journal of Applied Microbiology，2025，136（3）：lxaf041 ZHAO Z X，LI D M，TAN S M，et al. Colonization characteristics of Bacillus sp. Ya-1 and its effects on rhizosphere bacterial diversity of pepper[J]. Journal of Southern Agriculture，2024，55（11）：3346-3357 赵志祥，李道敏，谭诗梦，等. 芽孢杆菌Ya-1的定殖特性及其对辣椒根际细菌多样性的影响[J]. 南方农业学报，2024，55（11）：3346-3357 QIU J，BAI Y G，FU Y B，et al. Spatial variation in temperature thresholds during seed germination of remnant Festuca hallii populations across the Canadian prairie[J]. Environmental and Experimental Botany，2010，67（3）：479-486 GUO K Y，ZHANG K，YANG L，et al. Growth-promoting characteristics and fermentation optimization of Halomonas sp. L6 in cotton under saline-alkali stress[J]. Acta Agriculturae Boreali-occidentalis Sinica，2025，34（4）：639-652 郭珂妤，张凯，杨柳，等. 盐单胞菌L6对盐碱胁迫下棉花促生特性及发酵优化研究[J]. 西北农业学报，2025，34（4）：639-652 YU X. A study on phosphate-solubilizing bacteria of rhizosphere soil in walnut plant grown in Sichuan Province[D]. Yaan：Sichuan Agricultural University，2011：23-25 余旋. 四川核桃主产区根际解磷细菌研究[D]. 雅安：四川农业大学，2011：23-25 AHMAD H A，FARHANA L，HAROON U，et al. Rhizospheric bacterial strain Pseudomonas putida and fungal strain Penicillium chrysogenum alleviate Fusarium wilt of tomato by improving key growth attributes[J]. European Journal of Plant Pathology，2025，172（1）：123-142 AHMAD ANSARI F，AHMAD I，PICHTEL J，et al. Pantoea agglomerans FAP10： A novel biofilm-producing PGPR strain improves wheat growth and soil resilience under salinity stress[J]. Environmental and Experimental Botany，2024，222：105759 ZHANG Y Q，LI J M，CUI B H，et al. Research progress on the mechanism and application of rhizosphere microorganisms to the growth，development，and stress resistance of tobacco[J]. Biological Disaster Science，2024，47（4）：500-511 张玉芹，李军民，崔丙慧，等. 根际微生物对烟草生长发育及抗逆影响机制与应用研究进展[J]. 生物灾害科学，2024，47（4）：500-511 ZENG X L. Preliminary study on endophytic Herbaspirillum sp. ZXN111 colonization and growth-promoting activity on tea plant Camellia sinensis var. assamica[D]. Hefei：Anhui Agricultural University，2020：27-30 曾秀丽. 内生草螺菌ZXN111在阿萨姆种茶树中的定殖及促生作用初步研究[D]. 合肥：安徽农业大学，2020：27-30 ZHAO T L，DENG X L，XIAO Q Z，et al. IAA priming improves the germination and seedling growth in cotton （Gossypium hirsutum L.） via regulating the endogenous phytohormones and enhancing the sucrose metabolism[J]. Industrial Crops and Products，2020，155：112788 MILJAKOVIĆ D，MARINKOVIĆ J，TAMINDŽIĆ G，et al. Bio-priming of soybean with Bradyrhizobium japonicum and Bacillus megaterium： strategy to improve seed germination and the initial seedling growth[J]. Plants，2022，11（15）：1927 REN Z H，YU Q，GAO S K，et al. Screening and promoting effects of plant growth-promoting bacteria from rhizosphere of Campeiostachys nutans Griseb[J]. Acta Agrestia Sinica，2024，32（6）：1693-1701 任智慧，于乔，高士孔，等. 垂穗披碱草根际促生菌的分离鉴定及促生作用研究[J]. 草地学报，2024，32（6）：1693-1701 ZHAO S D，LI J H. The study on screening and characteristics of rhizosphere growth-promoting bacteria of Poa alpigena L[J]. Acta Agrestia Sinica，2021，29（9）：1885-1891 赵树栋，李建宏. 高原早熟禾根际促生菌分离筛选及特性研究[J]. 草地学报，2021，29（9）：1885-1891 QIAO S W，SHAO C，ZHANG H，et al. Effect on the seed germination and seedling growth of Glycyrrhiza uralensis under different concentration NaCl stress[J/OL]. https：//link.cnki.net/doi/10.16720/j.cnki.tcyj.2024.239，2024-12-16/2025-11-12 乔思薇，邵财，张浩，等. 不同浓度NaCl胁迫对甘草种子萌发和幼苗生长的影响[J/OL]. https：//link.cnki.net/doi/10.16720/j.cnki.tcyj.2024.239，2024-12-16/2025-11-12 SONG P，ZHAO B，SUN X X，et al. Effects of Bacillus subtilis HS5B5 on maize seed germination and seedling growth under NaCl stress conditions[J]. Agronomy，2023，13（7）：1874 MAKHAYE G，AREMU A O，GERRANO A S，et al. Biopriming with seaweed extract and microbial-based commercial biostimulants influences seed germination of five Abelmoschus esculentus genotypes[J]. Plants，2021，10（7）：1327 LI Y D，WANG H X，HU B G，et al. Research progress on plant growth promoting rhizobacteria to alleviate abiotic stress tolerance of horticultural crops[J]. Acta Horticulturae Sinica，2024，51（8）：1964-1976 李娅娣，王瀚祥，胡柏耿，等. 植物根际促生菌缓解园艺作物非生物胁迫研究进展[J]. 园艺学报，2024，51（8）：1964-1976 XIA T Q，ZHU X W，LU A G. Interactions of wheat PGPR and its effects on wheat growth[J]. Journal of Henan Agricultural Sciences，2009，38（5）：53-55 夏铁骑，朱学文，吕爱国. 小麦根际细菌的互作效应及其对小麦生长的影响[J]. 河南农业科学，2009，38（5）：53-55 ZHU Y X，ZHANG Y Y，WANG X Y，et al. Wolbachia modify host cell metabolite profiles in response to short-term temperature stress[J]. Environmental Microbiology Reports，2024，16（5）：e70013
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