Article(id=1309575099511038449, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.06.008, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750003200000, receivedDateStr=2025-06-16, revisedDate=1761580800000, revisedDateStr=2025-10-28, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156634452, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156634452, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156634452, creator=13701087609, updateTime=1790156634452, updator=13701087609, issue=Issue{id=1309575072856240766, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='6', pageStart='1963', pageEnd='2340', issueExtLink='null', onlineDate='null', pubDate='1781452800000', pubDateStr='2026-06-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156628097, creator='13701087609', updateTime=1790156954064, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576440438419817, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576440438419818, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2043, endPage=2054, ext={EN=ArticleExt(id=1309575099808834035, articleId=1309575099511038449, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Chicory (Cichorium intybus L.) is a perennial forage extensively utilized in livestock production. The magnesium-dechelatase involved in chlorophyll degradation plays a pivotal rolein regulating chlorophyll breakdown and is a key factor in the control of stay-green traits of plants. Enhancing the stay-green phenotype is critical for improving chicory’s resilience to environmental stresses. In this study, two putative SGR (Stay-Green) genes were preliminarily identified in the chicory genome, and designated as CiSGR and CiSGRL based on their respective phylogenetic affiliations to the SGR and SGRL subfamilies. The proteins encoded by these genes were composed of 274 and 232 amino acids, respectively. Structural domain analysis revealed that both CiSGR and CiSGRL possessed chloroplast transit peptide domains, highly conserved SGR domains, and variable regions at the C-terminus. Cis-regulatory element analysis indicated that the promoters of CiSGR and CiSGRL contained light-responsive, hormone-related, and stress-inducible transcriptional elements. Subcellular localization assays demonstrated that CiSGR predominantly localized to chloroplasts and nuclei, whereas CiSGRL primarily resided in nuclei and cytoplasm. Tissue-specific expression profiling showed the highest transcript levels of both genes in stems and significant induction under six different abiotic stress conditions. This systematic identification and analysis of chicory SGR and SGRL genes provides a theoretical foundation for strategies aimed at improving chicory forage yield and quality., authors=XIE Ming-yu1, LIU Xue2,3, WANG Chen-chen4, FENG Qing-xiang4, LI Da-yong2,3, ZHANG De-chun1, ZHANG Bin2,3, authorsList=XIE Ming-yu, LIU Xue, WANG Chen-chen, FENG Qing-xiang, LI Da-yong, ZHANG De-chun, ZHANG Bin, authorCompany=1 College of Biology and Pharmaceutical, China Three Gorges University, Yichang, Hubei Province 443000, China; 2 National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; 3 Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing 100097, China; 4 College of Life Sciences, Shandong Normal University, Jinan, Shandong Province 250014, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575099733336562, articleId=1309575099511038449, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=菊苣持绿基因CiSGR和CiSGRL的鉴定及胁迫条件下的表达分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=菊苣(Cichorium intybus L.)是一种被广泛用于畜牧业中的多年生牧草。持绿蛋白脱镁螯合酶参与调控叶绿素的分解,是植物调控持绿性状的关键因子,对提高菊苣的抗逆性至关重要。本研究在菊苣基因组中初步筛选到了2个菊苣SGR基因,这两个基因编码的蛋白质分别属于SGR亚家族和SGRL亚家族,分别将其命名为CiSGR和CiSGRL。二者编码的蛋白质分别由274和232个氨基酸组成。蛋白结构域分析表明,CiSGR和CiSGRL具有叶绿体转运肽结构域、高度保守的SGR结构域和C端可变区。顺式元件分析表明,CiSGR和CiSGRL的启动子含有光、激素和胁迫相关转录元件。亚细胞定位表明CiSGR主要定位于叶绿体和细胞核,CiSGRL主要定位于细胞核和细胞质。组织表达模式分析发现,CiSGR和CiSGRL均在茎中表达量最高,并受到6种不同胁迫信号的显著诱导。本研究对菊苣SGR和SGRL基因进行了系统鉴定和分析,为提升菊苣牧草的产量与品质提供理论依据。, authors=谢明宇1, 刘雪2,3, 王晨晨4, 冯清香4, 李大勇2,3, 张德春1, 张彬2,3, authorsList=谢明宇, 刘雪, 王晨晨, 冯清香, 李大勇, 张德春, 张彬, authorCompany=1 三峡大学生物与制药学院, 湖北 宜昌 443000; 2 北京市农林科学院蔬菜研究所, 国家蔬菜工程技术研究中心, 北京 100097; 3 北京市农林科学院蔬菜研究所, 作物分子设计与智慧育种北京市重点实验室, 北京 100097; 4 山东师范大学生命科学学院, 山东 济南 250014, correspAuthors=null, authorNote=谢明宇(2001-),男,满族,河北承德人,硕士研究生,主要从事植物基因组学研究,E-mail:921947629@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=tlouP0oDCLCWo+QAL+rsLA==, pdfFileSize=5956282, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=北京市农林科学院创新能力建设专项科技攻关项目 (KJCX20251008); 北京市农林科学院蔬菜研究所改革与发展项目 (KYCX202604))}, authors=[Author(id=1320569839169687823, tenantId=1146029695717560320, journalId=null, articleId=1309575099511038449, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={}, companyList=null)], keywords=[Keyword(id=1309575100039520756, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575099511038449, language=CN, orderNo=1, keyword=菊苣), Keyword(id=1309575100119212533, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575099511038449, language=CN, orderNo=2, keyword=SGR基因家族), 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fullTextJson=null, articleText=null, reference=WANG R N,CAO S X,ZHENG F,et al. Research and application of chicory as forage crop[J]. Feed Industry,2018,39(11):19-22 王若楠,曹升旭,郑飞,等. 菊苣作为饲用牧草的研究与应用[J]. 饲料工业,2018,39(11):19-22 KIDANE A,HOUDIJK J G M,ATHANASIADOU S,et al. Effects of maternal protein nutrition and subsequent grazing on chicory (Cichoriumintybus) on parasitism and performance of lambs[J]. Journal of Animal Science,2010,88(4):1513-1521 LIN Z J,WANG Y,GUO F F,et al. Analysis of CiteSpace knowledge map for Cichorium intybus[J]. China Journal of Chinese Materia Medica,2020,45(18):4490-4499 林志健,王雨,郭凡帆,等. 菊苣研究进展的CiteSpace知识图谱分析[J]. 中国中药杂志,2020,45(18):4490-4499 LOPES M S,REYNOLDS M P. Stay-green in spring wheat can be determined by spectral reflectance measurements (normalized difference vegetation index) independently from phenology[J]. Journal of Experimental Botany,2012,63(10):3789-3798 HU X Y,GU T Y,KHAN I,et al. Research progress in the interconversion, turnover and degradation of chlorophyll[J]. Cells,2021,10(11):3134 HÖRTENSTEINER S. Chlorophyll degradation during senescence[J]. Annual Review of Plant Biology,2006,57:55-77 CHEN Y Y,WANG S G,SUN D Z. The research progress in the function and regulation of STAYGREEN in crops[J]. Journal of Biology,2019,36(3):83-87 陈耀宇,王曙光,孙黛珍. 作物STAYGREEN功能与调控的研究进展[J]. 生物学杂志,2019,36(3):83-87 NICKELSEN J,RENGSTL B. Photosystem II assembly: from cyanobacteria to plants[J]. Annual Review of Plant Biology,2013,64:609-635 SAKURABA Y,HAN S H,LEE S H,et al. Arabidopsis NAC016 promotes chlorophyll breakdown by directly upregulating STAYGREEN1 transcription[J]. Plant Cell Reports,2016,35(1):155-166 JIAO B Z,MENG Q W,LÜ W. Roles of stay-green (SGR) homologs during chlorophyll degradation in green plants[J]. Botanical Studies,2020,61(1):25 WANG P,GRIMM B. Connecting chlorophyll metabolism with accumulation of the photosynthetic apparatus[J]. Trends in Plant Science,2021,26(5):484-495 ARMSTEAD I,DONNISON I,AUBRY S,et al. Cross-species identification of Mendel’s I locus[J]. Science,2007,315(5808):73 SAKURABA Y,PARK S Y,PAEK N C. The divergent roles of STAYGREEN (SGR) homologs in chlorophyll degradation[J]. Molecules and Cells,2015,38(5):390-395 JIANG H W,LI M R,LIANG N T,et al. Molecular cloning and function analysis of the stay green gene in rice[J]. The Plant Journal,2007,52(2):197-209 HU Z L,DENG L,YAN B,et al. Silencing of the LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype[J]. Biologia Plantarum,2011,55(1):27-34 BARRY C S,MCQUINN R P,CHUNG M Y,et al. Amino acid substitutions in homologs of the STAY-GREEN protein are responsible for the green-flesh and chlorophyll retainer mutations of tomato and pepper[J]. Plant Physiology,2008,147(1):179-187 SHI S Y,MIAO H Y,DU X M,et al. GmSGR1, a stay-green gene in soybean (Glycine max L.), plays an important role in regulating early leaf-yellowing phenotype and plant productivity under nitrogen deprivation[J]. Acta Physiologiae Plantarum,2016,38(4):97 LUO J,ABID M,ZHANG Y,et al. Genome-wide identification of kiwifruit SGR family members and functional characterization of SGR2 protein for chlorophyll degradation[J]. International Journal of Molecular Sciences,2023,24(3):1993 PARK S Y,YU J W,PARK J S,et al. The senescence-induced staygreen protein regulates chlorophyll degradation[J]. The Plant Cell,2007,19(5):1649-1664 YANG X T,ZHANG Z Q,XU L Y,et al. Plant chlorophyll degradation[J]. Plant Physiology Communications,2008,44(1):7-14 杨晓棠,张昭其,徐兰英,等. 植物叶绿素的降解[J]. 植物生理学通讯,2008,44(1):7-14 SHAO Y,ZHANG M M,CHEN Y,et al. Function identification of PpSGR gene and its regulation of ethylene synthesis in peach[J]. Journal of Fruit Science,2023,40(12):2513-2523 邵允,张蒙蒙,陈云,等. 桃PpSGR基因功能鉴定及其对乙烯合成的调控[J]. 果树学报,2023,40(12):2513-2523 BADE R G,BAO M L,JIN W Y,et al. Genome-wide identification and analysis of the SGR gene family in Cucumis melo L.[J]. Genetics and Molecular Research,2016,15(4):gmr15048485 XIE Z K,WU S D,CHEN J Y,et al. The C-terminal cysteine-rich motif of NYE1/SGR1 is indispensable for its function in chlorophyll degradation in Arabidopsis[J]. Plant Molecular Biology,2019,101(3):257-268 SHIN D,LEE S,KIM T H,et al. Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars[J]. Nature Communications,2020,11:2819 LUO Z D,ZHANG J H,LI J H,et al. A STAY-GREEN protein SlSGR1 regulates lycopene and β-carotene accumulation by interacting directly with SlPSY1 during ripening processes in tomato[J]. The New Phytologist,2013,198(2):442-452 REN G D,AN K,LIAO Y,et al. Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis[J]. Plant Physiology,2007,144(3):1429-1441 SAKURABA Y,PARK S Y,KIM Y S,et al. Arabidopsis STAYGREEN2 is a negative regulator of chlorophyll degradation during leaf senescence[J]. Molecular Plant,2014,7(8):1288-1302 WU S X,LI Z P,YANG L F,et al. NON-YELLOWING2 (NYE2),a close paralog of NYE1,plays a positive role in chlorophyll degradation in Arabidopsis[J]. Molecular Plant,2016,9(4):624-627 RONG H,TANG Y Y,ZHANG H,et al. The Stay-Green Rice like (SGRL) gene regulates chlorophyll degradation in rice[J]. Journal of Plant Physiology,2013,170(15):1367-1373 FANG C,LI C C,LI W Y,et al. Concerted evolution of D1 and D2 to regulate chlorophyll degradation in soybean[J]. The Plant Journal,2014,77(5):700-712 PAN J S,TAN J Y,WANG Y H,et al. STAYGREEN (CsSGR) is a candidate for the anthracnose (Colletotrichum orbiculare) resistance locus cla in Gy14 cucumber[J]. Theoretical and Applied Genetics,2018,131(7):1577-1587 ACEVEDO-GARCIA J,SPENCER D,THIERON H, et al. Mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach[J]. Plant Biotechnology Journal,2017,15(3):367-378 XIE W Y,XUE X,WANG Y,et al. Natural mutation in Stay-Green (OsSGR) confers enhanced resistance to rice sheath blight through elevating cytokinin content[J]. Plant Biotechnology Journal,2025,23(3):807-823 ISHIGA Y,RAO UPPALAPATI S,GILL U S,et al. Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust[J]. Scientific Reports,2015,5:13061 REN H Z,YU Y T,HUANG C,et al. Genome-wide identification and characterization of tea SGR family members reveal their potential roles in chlorophyll degradation and stress tolerance[J]. Agronomy,2024,14(4):769 ULUISIK S,KIYAK A,KURT F,et al. STAY-GREEN (SGR) genes in tomato (Solanum lycopersicum): genome-wide identification, and expression analyses reveal their involvements in ripening and salinity stress responses[J]. Horticulture,Environment,and Biotechnology,2022,63(4):557-569 ZHANG Y T,WEI G Q,XUE J Y,et al. CfSGR1 and CfSGR2 from Cryptomeria fortunei exhibit contrasting responses to hormones and abiotic stress in transgenic Arabidopsis[J]. Plant Physiology and Biochemistry,2024,216:109152 MAO Y Y,LI L J,LI J C, et al. Gene functional analysis of ZjSGR gene in Zoysia japonica leaves in response to vitro stress[J]. Acta Agrestia Sinica,2022,30(6):1396-1402 茅远煜,李丽菁,李进超,等. 结缕草ZjSGR基因在离体模拟逆境胁迫环境下的功能[J]. 草地学报,2022,30(6):1396-1402 DU Y,ZHANG Z W,LU S,et al. Chicory TCP gene family identification and analysis[J]. Acta Agrestia Sinica,2024,32(8):2428-2439 杜洋,张智韦,陆爽,等. 菊苣TCP基因家族鉴定与分析[J]. 草地学报,2024,32(8):2428-2439 SU H T,LIU M,YIN S X. Cloning, bioinformatics analysis and subcellular localization analysis of PpMYB2 gene in Poa pratensis[J]. Acta Agrestia Sinica,2024,32(10):3071-3079 苏浩天,刘曼,尹淑霞. 草地早熟禾PpMYB2基因的克隆、生物信息学及亚细胞定位分析[J]. 草地学报,2024,32(10):3071-3079 GUO R,ZHOU Z Y,CAI R, et al. Metabolomic and physiological analysis of alfalfa (Medicago sativa L.) in response to saline and alkaline stress[J]. Plant Physiology and Biochemistry,2024,207:108338 ACQUADRO A,BARCHI L,PORTIS E,et al. Genome reconstruction in cynara cardunculus taxa gains access to chromosome-scale DNA variation[J]. Scientific Reports,2017,7:5617 MAROUFI A,VAN BOCKSTAELE E,DE LOOSE M. Validation of reference genes for gene expression analysis in chicory(Cichorium intybus)using quantitative real-time PCR[J]. BMC Molecular Biology,2010,15:11-15 LIVAK K J,SCHMITTGEN T D L. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method[J]. Methods,2001,25(4):402-408 AGRAWAL D,JAJOO A. Study of high temperature stress induced damage and recovery in photosystem II (PSII) and photosystem I (PSI) in Spinach leaves (Spinacia oleracia)[J]. Journal of Plant Biochemistry and Biotechnology,2021,30(3):532-544 LU S,LIU Y Y,MAO J J,et al. Preliminary analysis on cold resistance of Zoysia japonica mutant plant Zjsgr in Beijing district[J]. Acta Agrestia Sinica,2025,33(1):28-33 陆爽,刘月月,毛佳静,等. 日本结缕草Zjsgr突变植株在北京地区的耐寒性初步分析[J]. 草地学报,2025,33(1):28-33)
Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory
Affiliations
1 College of Biology and Pharmaceutical, China Three Gorges University, Yichang, Hubei Province 443000, China; 2 National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; 3 Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing 100097, China; 4 College of Life Sciences, Shandong Normal University, Jinan, Shandong Province 250014, China
Chicory (Cichorium intybus L.) is a perennial forage extensively utilized in livestock production. The magnesium-dechelatase involved in chlorophyll degradation plays a pivotal rolein regulating chlorophyll breakdown and is a key factor in the control of stay-green traits of plants. Enhancing the stay-green phenotype is critical for improving chicory’s resilience to environmental stresses. In this study, two putative SGR (Stay-Green) genes were preliminarily identified in the chicory genome, and designated as CiSGR and CiSGRL based on their respective phylogenetic affiliations to the SGR and SGRL subfamilies. The proteins encoded by these genes were composed of 274 and 232 amino acids, respectively. Structural domain analysis revealed that both CiSGR and CiSGRL possessed chloroplast transit peptide domains, highly conserved SGR domains, and variable regions at the C-terminus. Cis-regulatory element analysis indicated that the promoters of CiSGR and CiSGRL contained light-responsive, hormone-related, and stress-inducible transcriptional elements. Subcellular localization assays demonstrated that CiSGR predominantly localized to chloroplasts and nuclei, whereas CiSGRL primarily resided in nuclei and cytoplasm. Tissue-specific expression profiling showed the highest transcript levels of both genes in stems and significant induction under six different abiotic stress conditions. This systematic identification and analysis of chicory SGR and SGRL genes provides a theoretical foundation for strategies aimed at improving chicory forage yield and quality.
WANG R N,CAO S X,ZHENG F,et al. Research and application of chicory as forage crop[J]. Feed Industry,2018,39(11):19-22 王若楠,曹升旭,郑飞,等. 菊苣作为饲用牧草的研究与应用[J]. 饲料工业,2018,39(11):19-22 KIDANE A,HOUDIJK J G M,ATHANASIADOU S,et al. Effects of maternal protein nutrition and subsequent grazing on chicory (Cichoriumintybus) on parasitism and performance of lambs[J]. Journal of Animal Science,2010,88(4):1513-1521 LIN Z J,WANG Y,GUO F F,et al. Analysis of CiteSpace knowledge map for Cichorium intybus[J]. China Journal of Chinese Materia Medica,2020,45(18):4490-4499 林志健,王雨,郭凡帆,等. 菊苣研究进展的CiteSpace知识图谱分析[J]. 中国中药杂志,2020,45(18):4490-4499 LOPES M S,REYNOLDS M P. Stay-green in spring wheat can be determined by spectral reflectance measurements (normalized difference vegetation index) independently from phenology[J]. Journal of Experimental Botany,2012,63(10):3789-3798 HU X Y,GU T Y,KHAN I,et al. Research progress in the interconversion, turnover and degradation of chlorophyll[J]. Cells,2021,10(11):3134 HÖRTENSTEINER S. Chlorophyll degradation during senescence[J]. Annual Review of Plant Biology,2006,57:55-77 CHEN Y Y,WANG S G,SUN D Z. The research progress in the function and regulation of STAYGREEN in crops[J]. Journal of Biology,2019,36(3):83-87 陈耀宇,王曙光,孙黛珍. 作物STAYGREEN功能与调控的研究进展[J]. 生物学杂志,2019,36(3):83-87 NICKELSEN J,RENGSTL B. Photosystem II assembly: from cyanobacteria to plants[J]. Annual Review of Plant Biology,2013,64:609-635 SAKURABA Y,HAN S H,LEE S H,et al. Arabidopsis NAC016 promotes chlorophyll breakdown by directly upregulating STAYGREEN1 transcription[J]. Plant Cell Reports,2016,35(1):155-166 JIAO B Z,MENG Q W,LÜ W. Roles of stay-green (SGR) homologs during chlorophyll degradation in green plants[J]. Botanical Studies,2020,61(1):25 WANG P,GRIMM B. Connecting chlorophyll metabolism with accumulation of the photosynthetic apparatus[J]. Trends in Plant Science,2021,26(5):484-495 ARMSTEAD I,DONNISON I,AUBRY S,et al. Cross-species identification of Mendel’s I locus[J]. Science,2007,315(5808):73 SAKURABA Y,PARK S Y,PAEK N C. The divergent roles of STAYGREEN (SGR) homologs in chlorophyll degradation[J]. Molecules and Cells,2015,38(5):390-395 JIANG H W,LI M R,LIANG N T,et al. Molecular cloning and function analysis of the stay green gene in rice[J]. The Plant Journal,2007,52(2):197-209 HU Z L,DENG L,YAN B,et al. Silencing of the LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype[J]. Biologia Plantarum,2011,55(1):27-34 BARRY C S,MCQUINN R P,CHUNG M Y,et al. Amino acid substitutions in homologs of the STAY-GREEN protein are responsible for the green-flesh and chlorophyll retainer mutations of tomato and pepper[J]. Plant Physiology,2008,147(1):179-187 SHI S Y,MIAO H Y,DU X M,et al. GmSGR1, a stay-green gene in soybean (Glycine max L.), plays an important role in regulating early leaf-yellowing phenotype and plant productivity under nitrogen deprivation[J]. Acta Physiologiae Plantarum,2016,38(4):97 LUO J,ABID M,ZHANG Y,et al. Genome-wide identification of kiwifruit SGR family members and functional characterization of SGR2 protein for chlorophyll degradation[J]. International Journal of Molecular Sciences,2023,24(3):1993 PARK S Y,YU J W,PARK J S,et al. The senescence-induced staygreen protein regulates chlorophyll degradation[J]. The Plant Cell,2007,19(5):1649-1664 YANG X T,ZHANG Z Q,XU L Y,et al. Plant chlorophyll degradation[J]. Plant Physiology Communications,2008,44(1):7-14 杨晓棠,张昭其,徐兰英,等. 植物叶绿素的降解[J]. 植物生理学通讯,2008,44(1):7-14 SHAO Y,ZHANG M M,CHEN Y,et al. Function identification of PpSGR gene and its regulation of ethylene synthesis in peach[J]. Journal of Fruit Science,2023,40(12):2513-2523 邵允,张蒙蒙,陈云,等. 桃PpSGR基因功能鉴定及其对乙烯合成的调控[J]. 果树学报,2023,40(12):2513-2523 BADE R G,BAO M L,JIN W Y,et al. Genome-wide identification and analysis of the SGR gene family in Cucumis melo L.[J]. Genetics and Molecular Research,2016,15(4):gmr15048485 XIE Z K,WU S D,CHEN J Y,et al. The C-terminal cysteine-rich motif of NYE1/SGR1 is indispensable for its function in chlorophyll degradation in Arabidopsis[J]. Plant Molecular Biology,2019,101(3):257-268 SHIN D,LEE S,KIM T H,et al. Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars[J]. Nature Communications,2020,11:2819 LUO Z D,ZHANG J H,LI J H,et al. A STAY-GREEN protein SlSGR1 regulates lycopene and β-carotene accumulation by interacting directly with SlPSY1 during ripening processes in tomato[J]. The New Phytologist,2013,198(2):442-452 REN G D,AN K,LIAO Y,et al. Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis[J]. Plant Physiology,2007,144(3):1429-1441 SAKURABA Y,PARK S Y,KIM Y S,et al. Arabidopsis STAYGREEN2 is a negative regulator of chlorophyll degradation during leaf senescence[J]. Molecular Plant,2014,7(8):1288-1302 WU S X,LI Z P,YANG L F,et al. NON-YELLOWING2 (NYE2),a close paralog of NYE1,plays a positive role in chlorophyll degradation in Arabidopsis[J]. Molecular Plant,2016,9(4):624-627 RONG H,TANG Y Y,ZHANG H,et al. The Stay-Green Rice like (SGRL) gene regulates chlorophyll degradation in rice[J]. Journal of Plant Physiology,2013,170(15):1367-1373 FANG C,LI C C,LI W Y,et al. Concerted evolution of D1 and D2 to regulate chlorophyll degradation in soybean[J]. The Plant Journal,2014,77(5):700-712 PAN J S,TAN J Y,WANG Y H,et al. STAYGREEN (CsSGR) is a candidate for the anthracnose (Colletotrichum orbiculare) resistance locus cla in Gy14 cucumber[J]. Theoretical and Applied Genetics,2018,131(7):1577-1587 ACEVEDO-GARCIA J,SPENCER D,THIERON H, et al. Mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach[J]. Plant Biotechnology Journal,2017,15(3):367-378 XIE W Y,XUE X,WANG Y,et al. Natural mutation in Stay-Green (OsSGR) confers enhanced resistance to rice sheath blight through elevating cytokinin content[J]. Plant Biotechnology Journal,2025,23(3):807-823 ISHIGA Y,RAO UPPALAPATI S,GILL U S,et al. Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust[J]. Scientific Reports,2015,5:13061 REN H Z,YU Y T,HUANG C,et al. Genome-wide identification and characterization of tea SGR family members reveal their potential roles in chlorophyll degradation and stress tolerance[J]. Agronomy,2024,14(4):769 ULUISIK S,KIYAK A,KURT F,et al. STAY-GREEN (SGR) genes in tomato (Solanum lycopersicum): genome-wide identification, and expression analyses reveal their involvements in ripening and salinity stress responses[J]. Horticulture,Environment,and Biotechnology,2022,63(4):557-569 ZHANG Y T,WEI G Q,XUE J Y,et al. CfSGR1 and CfSGR2 from Cryptomeria fortunei exhibit contrasting responses to hormones and abiotic stress in transgenic Arabidopsis[J]. Plant Physiology and Biochemistry,2024,216:109152 MAO Y Y,LI L J,LI J C, et al. Gene functional analysis of ZjSGR gene in Zoysia japonica leaves in response to vitro stress[J]. Acta Agrestia Sinica,2022,30(6):1396-1402 茅远煜,李丽菁,李进超,等. 结缕草ZjSGR基因在离体模拟逆境胁迫环境下的功能[J]. 草地学报,2022,30(6):1396-1402 DU Y,ZHANG Z W,LU S,et al. Chicory TCP gene family identification and analysis[J]. Acta Agrestia Sinica,2024,32(8):2428-2439 杜洋,张智韦,陆爽,等. 菊苣TCP基因家族鉴定与分析[J]. 草地学报,2024,32(8):2428-2439 SU H T,LIU M,YIN S X. Cloning, bioinformatics analysis and subcellular localization analysis of PpMYB2 gene in Poa pratensis[J]. Acta Agrestia Sinica,2024,32(10):3071-3079 苏浩天,刘曼,尹淑霞. 草地早熟禾PpMYB2基因的克隆、生物信息学及亚细胞定位分析[J]. 草地学报,2024,32(10):3071-3079 GUO R,ZHOU Z Y,CAI R, et al. Metabolomic and physiological analysis of alfalfa (Medicago sativa L.) in response to saline and alkaline stress[J]. Plant Physiology and Biochemistry,2024,207:108338 ACQUADRO A,BARCHI L,PORTIS E,et al. Genome reconstruction in cynara cardunculus taxa gains access to chromosome-scale DNA variation[J]. Scientific Reports,2017,7:5617 MAROUFI A,VAN BOCKSTAELE E,DE LOOSE M. Validation of reference genes for gene expression analysis in chicory(Cichorium intybus)using quantitative real-time PCR[J]. BMC Molecular Biology,2010,15:11-15 LIVAK K J,SCHMITTGEN T D L. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method[J]. Methods,2001,25(4):402-408 AGRAWAL D,JAJOO A. Study of high temperature stress induced damage and recovery in photosystem II (PSII) and photosystem I (PSI) in Spinach leaves (Spinacia oleracia)[J]. Journal of Plant Biochemistry and Biotechnology,2021,30(3):532-544 LU S,LIU Y Y,MAO J J,et al. Preliminary analysis on cold resistance of Zoysia japonica mutant plant Zjsgr in Beijing district[J]. Acta Agrestia Sinica,2025,33(1):28-33 陆爽,刘月月,毛佳静,等. 日本结缕草Zjsgr突变植株在北京地区的耐寒性初步分析[J]. 草地学报,2025,33(1):28-33