Article(id=1309575077864235450, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.06.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1755619200000, receivedDateStr=2025-08-20, revisedDate=1760457600000, revisedDateStr=2025-10-15, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156629291, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156629291, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156629291, creator=13701087609, updateTime=1790156629291, updator=13701087609, issue=Issue{id=1309575072856240766, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='6', pageStart='1963', pageEnd='2340', issueExtLink='null', onlineDate='null', pubDate='1781452800000', pubDateStr='2026-06-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156628097, creator='13701087609', updateTime=1790156954064, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576440438419817, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576440438419818, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309575072856240766, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2022, endPage=2031, ext={EN=ArticleExt(id=1309575078669541820, articleId=1309575077864235450, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Laccase （LAC）， as an important member of the ceruloplasmin protein family， plays a key role in the regulation of plant growth and development and the response to abiotic stress. In this study， the local superior germplasm of Medicago sativa ‘Pianguan’ in Shanxi Province was used as the experimental material. The MsLAC17 gene was cloned by PCR. And was analyzed by bioinformatics means and fluorescence quantitative PCR technology. The tissue specificity expression of MsLAC17 gene and its response under different abiotic stresses were systematically explored. The results showed that the length of the MsLAC17 gene is 1782 bp， encoding 593 amino acids. The relative molecular mass of this protein is 66.26 kDa， and its theoretical isoelectric point is 10.05. It is a hydrophilic protein mainly located in chloroplasts and mitochondria. The results of phylogenetic analysis indicated that the LAC17 protein of Medicago sativa ‘Pianguan’ is closely related to that of Medicago truncatula. Fluorescence quantitative PCR detection revealed that the expression level of the MsLAC17 gene was the highest in the stem， and responded to salt， drought and ABA treatment. It is speculated that this gene may play a role in regulating the response of Medicago sativa ‘Pianguan’ to abiotic stress. The results of this study provide an important basis for further clarifying the biological function of the MsLAC17 gene., authors=DU Xin-wei1, RONG Rui-wei1, HUA Yi-wen1, LIU Yi1, TAO Jia-li1,2, ZHOU Si-yi1, XU Tao1, CEN Hui-fang1, ZHU Hui-sen1, authorsList=DU Xin-wei, RONG Rui-wei, HUA Yi-wen, LIU Yi, TAO Jia-li, ZHOU Si-yi, XU Tao, CEN Hui-fang, ZHU Hui-sen, authorCompany=1 College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China; 2 Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575078598238651, articleId=1309575077864235450, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=偏关苜蓿MsLAC17基因克隆及其对非生物胁迫的响应分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=漆酶（Laccase，LAC）作为铜蓝氧化酶蛋白家族的重要成员，在植物生长发育调控及非生物胁迫响应过程中扮演着关键角色。本研究选取山西地方优良种质‘偏关’苜蓿（Medicago sativa ‘Pianguan’）作为试验材料，采用PCR技术克隆获得MsLAC17基因，通过生物信息学手段对该基因进行分析，并利用荧光定量PCR技术，系统探究了MsLAC17基因表达的组织特异性，及其在不同非生物胁迫下的响应情况。结果显示：MsLAC17基因全长1782 bp，编码593个氨基酸，该蛋白的相对分子质量为66.26 kDa，理论等电点为10.05，属于亲水性蛋白，主要位于叶绿体和线粒体中。系统进化分析结果表明，‘偏关’苜蓿LAC17蛋白与蒺藜苜蓿（Medicago truncatula）的LAC17蛋白亲缘关系较近。荧光定量PCR检测发现MsLAC17基因在‘偏关’苜蓿茎中表达量最高，且对盐、干旱和ABA处理均有响应，推测该基因可能在调控‘偏关’苜蓿应对非生物胁迫的过程中发挥作用，本研究结果为进一步明晰MsLAC17基因的生物学功能奠定理论基础。, authors=杜欣蔚1, 戎睿伟1, 华怡雯1, 刘怡1, 陶佳丽1,2, 周思懿1, 许涛1, 岑慧芳1, 朱慧森1, authorsList=杜欣蔚, 戎睿伟, 华怡雯, 刘怡, 陶佳丽, 周思懿, 许涛, 岑慧芳, 朱慧森, authorCompany=1 山西农业大学草业学院, 山西 太谷 030801; 2 山西工程技术学院矿业工程系, 山西 阳泉 045000, correspAuthors=null, authorNote=杜欣蔚(2000-),女,汉族,山东淄博人,硕士研究生,主要从事牧草种质资源与育种相关研究,E-mail:dxwxiamo@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=cfA5Wtq8/71/7+DDDh5U8Q==, pdfFileSize=8321694, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32071872); 山西省中央引导地方科技发展资金项目 (YDZJSX2022B006); 现代种业发展项目 (sxzyk202501-7); 山西省重点研发计划课题 (202102140601006-3); 山西省基础研究计划 (202303021221101); 山西省来晋科研奖励 (SXBYKY2022118); 优秀博士启动项目 (2021BQ01,2023BQ03); 山西省研究生实践创新项目 (2024SJ133); 山西省研究生学术创新项目 (2025XS300))}, authors=null, keywords=[Keyword(id=1309575078946365885, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575077864235450, language=CN, orderNo=1, keyword=‘偏关’苜蓿), 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pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.06.006, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1781452800000, fullTextJson=null, articleText=null, reference=WANG Z L，HANG W G，WANG X Q，et al. Comparative study on production performance of 10 alfalfa species in the moderate and mild saline alkali land in Jiuquan Desert irrigation area[J]. Journal of Animal Science and Veterinary Medicine，2021，40（6）：14-17，20 王志龙，黄卫国，王新强，等. 10种紫花苜蓿在酒泉荒漠灌区中轻度盐碱地生产性能比较研究[J]. 畜牧兽医杂志，2021，40（6）：14-17，20 CHEN C R. Chinese pharmacopoeia dictionary （volume 1）[M]. Shanghai：World Publishing House，1935：896 陈存仁. 中国药学大辞典（上册）[M]. 上海：世界书局，1935：896 BIAN J H，LIU Z Y，LI Z Y，et al. Advances in alfalfa improvement using genetic engineering[J]. Pratacultural Science，2020，37（1）：139-155 边佳辉，刘自扬，李宗英，等. 紫花苜蓿基因工程改良研究进展[J]. 草业科学，2020，37（1）：139-155 ZHANG M，LIU S，LIU Y，et al. Soil moisture variation characteristics of different land use types in the moderate slope sandy area of Loess Hilly Region[J]. Journal of Soil and Water Conservation，2019，33（3）：115-120，128 张敏，刘爽，刘勇，等. 黄土丘陵缓坡风沙区不同土地利用类型土壤水分变化特征[J]. 水土保持学报，2019，33（3）：115-120，128 ZHANG J Y，ZHANG Z，WANG J，et al. Advances in alfalfa stress resistance breeding[J]. China Herbivore Science，2025，45（2）：80-85 张杰一，张众，王静，等. 紫花苜蓿抗逆育种研究进展[J]. 中国草食动物科学，2025，45（2）：80-85 HOEGGER P J，KILARU S，JAMES T Y，et al. Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences[J]. The FEBS Journal，2006，273（10）：2308-2326 LIU Q Q，LUO L，WANG X X，et al. Comprehensive analysis of rice laccase gene （OsLAC） family and ectopic expression of OsLAC10 enhances tolerance to copper stress in Arabidopsis[J]. International Journal of Molecular Sciences，2017，18（2）：209 BERTHET S，DEMONT-CAULET N，POLLET B，et al. Disruption of LACCASE4 and 17 results in tissue-specific alterations to lignification of Arabidopsis thaliana stems[J]. The Plant Cell，2011，23（3）：1124-1137 ZHUO C L，WANG X，DOCAMPO-PALACIOS M，et al. Developmental changes in lignin composition are driven by both monolignol supply and laccase specificity[J]. Science Advances，2022，8（10）：eabm8145 CAI X N，DAVIS E J，BALLIF J，et al. Mutant identification and characterization of the laccase gene family in Arabidopsis[J]. Journal of Experimental Botany，2006，57（11）：2563-2569 XU X，ZHOU Y P，WANG B，et al. Genome-wide identification and characterization of laccase gene family in Citrus sinensis[J]. Gene，2019，689：114-123 NIU Z M，LI G T，HU H Y，et al. A gene that underwent adaptive evolution， LAC2 （LACCASE）， in Populus euphratica improves drought tolerance by improving water transport capacity[J]. Horticulture Research，2021，8：88 XU X，ZHANG Y L，LIANG M G，et al. The Citrus laccase gene CsLAC18 contributes to cold tolerance[J]. International Journal of Molecular Sciences，2022，23（23）：14509 LI L，YANG K B，WANG S N，et al. Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses[J]. Plant Cell Reports，2020，39（6）：751-763 ZHUANG Y，ZUO D Q，TAO Y H，et al. Laccase3-based extracellular domain provides possible positional information for directing Casparian strip formation in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America，2020，117（27）：15400-15402 BAI Y S，ZHOU J J，TANG Y L. Plant laccases and their roles in plant growth and development[J]. Chinese Bulletin of Life Sciences，2022，34（9）：1135-1144 白永生，周嘉杰，唐玉林. 植物漆酶及其在植物生长发育中的作用[J]. 生命科学，2022，34（9）：1135-1144 ZHAO J Y，LI X Y，ZHU H S，et al. Seed germination and plant regeneration system optimization of Medicago sativa ‘Pianguan’[J]. Acta Agrestia Sinica，2020，28（4）：947-955 赵娇阳，李小艳，朱慧森，等. 偏关苜蓿种子萌发及植株再生体系优化[J]. 草地学报，2020，28（4）：947-955 SHEN C，DU H L，CHEN Z，et al. The chromosome-level genome sequence of the autotetraploid alfalfa and resequencing of core germplasms provide genomic resources for alfalfa research[J]. Molecular Plant，2020，13（9）：1250-1261 LONG R C，ZHANG F，ZHANG Z W，et al. Genome assembly of alfalfa cultivar Zhongmu-4 and identification of SNPs associated with agronomic traits[J]. Genomics， Proteomics & Bioinformatics，2022，20（1）：14-28 LIVAK K J，SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt Method[J]. Methods，2001，25（4）：402-408 GIARDINA P，FARACO V，PEZZELLA C，et al. Laccases： a never-ending story[J]. Cellular and Molecular Life Sciences，2010，67（3）：369-385 MOT A C，SILAGHI-DUMITRESCU R. Laccases： complex architectures for one-electron oxidations[J]. Biochemistry（Moscow），2012，77（12）：1395-1407 MUNK L，SITARZ A K，KALYANI D C，et al. Can laccases catalyze bond cleavage in lignin？[J]. Biotechnology Advances，2015，33（1）：13-24 TIAN Q L，LIN Y L，ZHENG Q Y，et al. Cloning and expression analyses of DlLac7 in Dimocarpus longan[J]. Journal of Fruit Science，2016，33（10）：1185-1193 田奇琳，林玉玲，郑庆游，等. 龙眼DlLac7的克隆及其表达调控分析[J]. 果树学报，2016，33（10）：1185-1193 HOFFMANN N，BENSKE A，BETZ H，et al. Laccases and peroxidases co-localize in lignified secondary cell walls throughout stem development[J]. Plant Physiology，2020，184（2）：806-822 PERKINS M L，SCHUETZ M，UNDA F，et al. Dwarfism of high-monolignol Arabidopsis plants is rescued by ectopic LACCASE overexpression[J]. Plant Direct，2020，4（9）：e00265 ZHANG S C，JU C L，WANG X J. Arabidopsis laccase gene AtLAC4 regulates plant growth and responses to abiotic stress[J]. Chinese Bulletin of Botany，2012，47（4）：357-365 张盛春，鞠常亮，王小菁. 拟南芥漆酶基因AtLAC4参与生长及非生物胁迫响应[J]. 植物学报，2012，47（4）：357-365 ZHAO X Y，PANG M L，ZHAO Q，et al. Cloning and expression analysis of tomato LeLACmiR397 gene[J]. Acta Horticulturae Sinica，2015，42（7）：1285-1298 赵先炎，庞明利，赵强，等. 番茄漆酶基因LeLACmiR397的克隆与表达分析[J]. 园艺学报，2015，42（7）：1285-1298 LIANG M，HAROLDSEN V，CAI X N，et al. Expression of a putative laccase gene， ZmLAC1， in maize primary roots under stress[J]. Plant， Cell & Environment，2006，29（5）：746-753 BAI Y，ALI S，LIU S，et al. Characterization of plant laccase genes and their functions[J]. Gene，2023，852：147060 GU M Z. Identification of laccase genes from Poncirus trifoliata under salt stress and regulation by exogenous methyl jasmonate[D]. Yangzhou：Yangzhou University，2023：33 顾铭洲. 盐胁迫下枳漆酶基因的鉴定及外源茉莉酸甲酯的调节作用研究[D]. 扬州：扬州大学，2023：33 CHENG T，REN C Y，XU J H，et al. Genome-wide analysis of the common bean （Phaseolus vulgaris） laccase gene family and its functions in response to abiotic stress[J]. BMC Plant Biology，2024，24（1）：688 TURLAPATI P V，KIM K W，DAVIN L B，et al. The laccase multigene family in Arabidopsis thaliana： towards addressing the mystery of their gene function（s）[J]. Planta，2011，233（3）：439-470)
Cloning MsLAC17 Gene and Analysis of its Response to Abiotic Stress in Medicago sativa ‘Pianguan’
DU Xin-wei1, RONG Rui-wei1, HUA Yi-wen1, LIU Yi1, TAO Jia-li1,2, ZHOU Si-yi1, XU Tao1, CEN Hui-fang1, ZHU Hui-sen1
Affiliations
1 College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China; 2 Department of Mining Engineering, Shanxi Institute of Technology, Yangquan, Shanxi Province 045000, China
Laccase （LAC）， as an important member of the ceruloplasmin protein family， plays a key role in the regulation of plant growth and development and the response to abiotic stress. In this study， the local superior germplasm of Medicago sativa ‘Pianguan’ in Shanxi Province was used as the experimental material. The MsLAC17 gene was cloned by PCR. And was analyzed by bioinformatics means and fluorescence quantitative PCR technology. The tissue specificity expression of MsLAC17 gene and its response under different abiotic stresses were systematically explored. The results showed that the length of the MsLAC17 gene is 1782 bp， encoding 593 amino acids. The relative molecular mass of this protein is 66.26 kDa， and its theoretical isoelectric point is 10.05. It is a hydrophilic protein mainly located in chloroplasts and mitochondria. The results of phylogenetic analysis indicated that the LAC17 protein of Medicago sativa ‘Pianguan’ is closely related to that of Medicago truncatula. Fluorescence quantitative PCR detection revealed that the expression level of the MsLAC17 gene was the highest in the stem， and responded to salt， drought and ABA treatment. It is speculated that this gene may play a role in regulating the response of Medicago sativa ‘Pianguan’ to abiotic stress. The results of this study provide an important basis for further clarifying the biological function of the MsLAC17 gene.
WANG Z L，HANG W G，WANG X Q，et al. Comparative study on production performance of 10 alfalfa species in the moderate and mild saline alkali land in Jiuquan Desert irrigation area[J]. Journal of Animal Science and Veterinary Medicine，2021，40（6）：14-17，20 王志龙，黄卫国，王新强，等. 10种紫花苜蓿在酒泉荒漠灌区中轻度盐碱地生产性能比较研究[J]. 畜牧兽医杂志，2021，40（6）：14-17，20 CHEN C R. Chinese pharmacopoeia dictionary （volume 1）[M]. Shanghai：World Publishing House，1935：896 陈存仁. 中国药学大辞典（上册）[M]. 上海：世界书局，1935：896 BIAN J H，LIU Z Y，LI Z Y，et al. Advances in alfalfa improvement using genetic engineering[J]. Pratacultural Science，2020，37（1）：139-155 边佳辉，刘自扬，李宗英，等. 紫花苜蓿基因工程改良研究进展[J]. 草业科学，2020，37（1）：139-155 ZHANG M，LIU S，LIU Y，et al. Soil moisture variation characteristics of different land use types in the moderate slope sandy area of Loess Hilly Region[J]. Journal of Soil and Water Conservation，2019，33（3）：115-120，128 张敏，刘爽，刘勇，等. 黄土丘陵缓坡风沙区不同土地利用类型土壤水分变化特征[J]. 水土保持学报，2019，33（3）：115-120，128 ZHANG J Y，ZHANG Z，WANG J，et al. Advances in alfalfa stress resistance breeding[J]. China Herbivore Science，2025，45（2）：80-85 张杰一，张众，王静，等. 紫花苜蓿抗逆育种研究进展[J]. 中国草食动物科学，2025，45（2）：80-85 HOEGGER P J，KILARU S，JAMES T Y，et al. Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences[J]. The FEBS Journal，2006，273（10）：2308-2326 LIU Q Q，LUO L，WANG X X，et al. Comprehensive analysis of rice laccase gene （OsLAC） family and ectopic expression of OsLAC10 enhances tolerance to copper stress in Arabidopsis[J]. International Journal of Molecular Sciences，2017，18（2）：209 BERTHET S，DEMONT-CAULET N，POLLET B，et al. Disruption of LACCASE4 and 17 results in tissue-specific alterations to lignification of Arabidopsis thaliana stems[J]. The Plant Cell，2011，23（3）：1124-1137 ZHUO C L，WANG X，DOCAMPO-PALACIOS M，et al. Developmental changes in lignin composition are driven by both monolignol supply and laccase specificity[J]. Science Advances，2022，8（10）：eabm8145 CAI X N，DAVIS E J，BALLIF J，et al. Mutant identification and characterization of the laccase gene family in Arabidopsis[J]. Journal of Experimental Botany，2006，57（11）：2563-2569 XU X，ZHOU Y P，WANG B，et al. Genome-wide identification and characterization of laccase gene family in Citrus sinensis[J]. Gene，2019，689：114-123 NIU Z M，LI G T，HU H Y，et al. A gene that underwent adaptive evolution， LAC2 （LACCASE）， in Populus euphratica improves drought tolerance by improving water transport capacity[J]. Horticulture Research，2021，8：88 XU X，ZHANG Y L，LIANG M G，et al. The Citrus laccase gene CsLAC18 contributes to cold tolerance[J]. International Journal of Molecular Sciences，2022，23（23）：14509 LI L，YANG K B，WANG S N，et al. Genome-wide analysis of laccase genes in moso bamboo highlights PeLAC10 involved in lignin biosynthesis and in response to abiotic stresses[J]. Plant Cell Reports，2020，39（6）：751-763 ZHUANG Y，ZUO D Q，TAO Y H，et al. Laccase3-based extracellular domain provides possible positional information for directing Casparian strip formation in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America，2020，117（27）：15400-15402 BAI Y S，ZHOU J J，TANG Y L. Plant laccases and their roles in plant growth and development[J]. Chinese Bulletin of Life Sciences，2022，34（9）：1135-1144 白永生，周嘉杰，唐玉林. 植物漆酶及其在植物生长发育中的作用[J]. 生命科学，2022，34（9）：1135-1144 ZHAO J Y，LI X Y，ZHU H S，et al. Seed germination and plant regeneration system optimization of Medicago sativa ‘Pianguan’[J]. Acta Agrestia Sinica，2020，28（4）：947-955 赵娇阳，李小艳，朱慧森，等. 偏关苜蓿种子萌发及植株再生体系优化[J]. 草地学报，2020，28（4）：947-955 SHEN C，DU H L，CHEN Z，et al. The chromosome-level genome sequence of the autotetraploid alfalfa and resequencing of core germplasms provide genomic resources for alfalfa research[J]. Molecular Plant，2020，13（9）：1250-1261 LONG R C，ZHANG F，ZHANG Z W，et al. Genome assembly of alfalfa cultivar Zhongmu-4 and identification of SNPs associated with agronomic traits[J]. Genomics， Proteomics & Bioinformatics，2022，20（1）：14-28 LIVAK K J，SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt Method[J]. Methods，2001，25（4）：402-408 GIARDINA P，FARACO V，PEZZELLA C，et al. Laccases： a never-ending story[J]. Cellular and Molecular Life Sciences，2010，67（3）：369-385 MOT A C，SILAGHI-DUMITRESCU R. Laccases： complex architectures for one-electron oxidations[J]. Biochemistry（Moscow），2012，77（12）：1395-1407 MUNK L，SITARZ A K，KALYANI D C，et al. Can laccases catalyze bond cleavage in lignin？[J]. Biotechnology Advances，2015，33（1）：13-24 TIAN Q L，LIN Y L，ZHENG Q Y，et al. Cloning and expression analyses of DlLac7 in Dimocarpus longan[J]. Journal of Fruit Science，2016，33（10）：1185-1193 田奇琳，林玉玲，郑庆游，等. 龙眼DlLac7的克隆及其表达调控分析[J]. 果树学报，2016，33（10）：1185-1193 HOFFMANN N，BENSKE A，BETZ H，et al. Laccases and peroxidases co-localize in lignified secondary cell walls throughout stem development[J]. Plant Physiology，2020，184（2）：806-822 PERKINS M L，SCHUETZ M，UNDA F，et al. Dwarfism of high-monolignol Arabidopsis plants is rescued by ectopic LACCASE overexpression[J]. Plant Direct，2020，4（9）：e00265 ZHANG S C，JU C L，WANG X J. Arabidopsis laccase gene AtLAC4 regulates plant growth and responses to abiotic stress[J]. Chinese Bulletin of Botany，2012，47（4）：357-365 张盛春，鞠常亮，王小菁. 拟南芥漆酶基因AtLAC4参与生长及非生物胁迫响应[J]. 植物学报，2012，47（4）：357-365 ZHAO X Y，PANG M L，ZHAO Q，et al. Cloning and expression analysis of tomato LeLACmiR397 gene[J]. Acta Horticulturae Sinica，2015，42（7）：1285-1298 赵先炎，庞明利，赵强，等. 番茄漆酶基因LeLACmiR397的克隆与表达分析[J]. 园艺学报，2015，42（7）：1285-1298 LIANG M，HAROLDSEN V，CAI X N，et al. Expression of a putative laccase gene， ZmLAC1， in maize primary roots under stress[J]. Plant， Cell & Environment，2006，29（5）：746-753 BAI Y，ALI S，LIU S，et al. Characterization of plant laccase genes and their functions[J]. Gene，2023，852：147060 GU M Z. Identification of laccase genes from Poncirus trifoliata under salt stress and regulation by exogenous methyl jasmonate[D]. Yangzhou：Yangzhou University，2023：33 顾铭洲. 盐胁迫下枳漆酶基因的鉴定及外源茉莉酸甲酯的调节作用研究[D]. 扬州：扬州大学，2023：33 CHENG T，REN C Y，XU J H，et al. Genome-wide analysis of the common bean （Phaseolus vulgaris） laccase gene family and its functions in response to abiotic stress[J]. BMC Plant Biology，2024，24（1）：688 TURLAPATI P V，KIM K W，DAVIN L B，et al. The laccase multigene family in Arabidopsis thaliana： towards addressing the mystery of their gene function（s）[J]. Planta，2011，233（3）：439-470
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