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2 College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575076681441712, articleId=1309575074357797291, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=MYB-related基因家族在植物生长发育及逆境胁迫中起关键作用。本研究基于沙木蓼(Atraphaxis bracteata)全长转录组数据,采用生物信息学方法鉴定了其MYB-related基因家族,分析了进化关系、基因结构、理化性质,并采用qRT-PCR分析了其在干旱胁迫下各组织的时空表达模式。结果显示,共鉴定出55个成员,分为5个亚组;编码蛋白多呈亲水性,主要定位于细胞核,Motif 2为特征基序,Motif 4保守性最高,蛋白质二、三级结构以无规则卷曲为主。表达分析表明,干旱胁迫24 h后基因表达量均高于6 h和0 h,多数基因在叶中表达较高,这些基因在根茎中对重度干旱响应更强,而在叶中则对轻度干旱更为敏感。综上,沙木蓼MYB-related基因家族在干旱胁迫中具有组织特异性表达模式,为今后功能研究与抗逆育种提供了理论依据。, authors=何彩1, 刘源2, 晋敏1, 吴媛1, 王素桢1, 胡芳1, authorsList=何彩, 刘源, 晋敏, 吴媛, 王素桢, 胡芳, authorCompany=1 武威市林业科学研究院, 甘肃 武威 733000;
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HE C,JIN M,LIU W,et al. The metabolomics and transcriptomics of Atraphaxis bracteata reveal age-related molecular alterations under naturally high temperature and drought conditions[J]. Physiologia Plantarum,2024,176(1):e14129
HE C,LIU W,JIN M,et al. Analysis of full length transcriptome and resistance characteristics of Atraphaxis bracteata under drought[J]. Scientific Reports,2025,15:807
ZHANG Z X,GUO X W,WANG Z W,et al. Identification and expression analysis of NAC gene family in Cerasus humilis[J]. Journal of Fruit Science,2023,40(2):206-222 张忠鑫,郭夕雯,汪泽文,等. 欧李NAC基因家族的鉴定及表达分析[J]. 果树学报,2023,40(2):206-222
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ZHAO X,LI Y M,LI Y M,et al. Identification and analysis of R1-MYB gene family in Rheum palmatum L. based on full-length transcriptome sequencing[J]. Acta Pharmaceutica Sinica,2023,58(5):1354-1363 赵霞,李元敏,李依民,等. 基于全长转录组测序的掌叶大黄R1-MYB基因家族鉴定分析[J]. 药学学报,2023,58(5):1354-1363
JIA G Q,LI B,ZHANG D F,et al. Cloning and characterization of a novel R1-MYB transcription factor in maize[J]. Progress in Natural Science,2009,19(9):1089-1096
WANG C P,CHEN J W,YAN L,et al. Cloning and expression analysis of R1-MYB transcription factor in Lycium ruthenicum[J]. Chinese Traditional and Herbal Drugs,2018,49(1):203-210 王翠平,陈建伟,严莉,等. 黑果枸杞R1-MYB转录因子基因的克隆及表达分析[J]. 中草药,2018,49(1):203-210
FAN J S B,ZHONG X X,SHAN T Y,et al. Identification and analysis of 1R-MYB transcription factors regulating the phenylpropane biosynthesis pathway in Ranunculus Japonicus Thunb[J]. Plant Science Journal,2024,42(4):478-487 樊金世博,钟欣欣,单婷玉,等. 毛茛中与苯丙烷生物合成途径相关的1R-MYB类转录因子的鉴定与分析[J]. 植物科学学报,2024,42(4):478-487)
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沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究
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草地学报 | 研究论文 2026,34(6): 2012-2021
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草地学报 |研究论文 2026 , 34 (6) : 2012 -2021
沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究
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何彩1, 刘源2, 晋敏1, 吴媛1, 王素桢1, 胡芳1
作者信息
    1 武威市林业科学研究院, 甘肃 武威 733000;
    2 甘肃农业大学林学院, 甘肃 兰州 730000
作者简介:
何彩(1985-),女,汉族,甘肃武威人,博士,正高级工程师,主要从事荒漠化防治研究,E-mail:hcyldfcl@163.com
Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress
  • HE Cai1, LIU Yuan2, JIN Min1, WU Yuan1, WANG Su-zhen1, HU Fang1
  • Affiliations
      1 Wuwei Academy of Forestry, Wuwei, Gansu Province 733000, China;
      2 College of Forestry, Gansu Agricultural University, Lanzhou, Gansu Province 730000, China
    doi: 10.11733/j.issn.1007-0435.2026.06.005
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    MYB-related基因家族在植物生长发育及逆境胁迫中起关键作用。本研究基于沙木蓼(Atraphaxis bracteata)全长转录组数据,采用生物信息学方法鉴定了其MYB-related基因家族,分析了进化关系、基因结构、理化性质,并采用qRT-PCR分析了其在干旱胁迫下各组织的时空表达模式。结果显示,共鉴定出55个成员,分为5个亚组;编码蛋白多呈亲水性,主要定位于细胞核,Motif 2为特征基序,Motif 4保守性最高,蛋白质二、三级结构以无规则卷曲为主。表达分析表明,干旱胁迫24 h后基因表达量均高于6 h和0 h,多数基因在叶中表达较高,这些基因在根茎中对重度干旱响应更强,而在叶中则对轻度干旱更为敏感。综上,沙木蓼MYB-related基因家族在干旱胁迫中具有组织特异性表达模式,为今后功能研究与抗逆育种提供了理论依据。
    沙木蓼  /  MYB-related  /  干旱胁迫  /  表达模式
    MYB-related gene family plays important roles in plant growth, development, and stress response. This study analyzed the number, evolutionary relationships, gene structure, and physicochemical properties of MYB-related family genes in Atraphaxis bracteata by bioinformatics methods based on the full-length transcriptome data of A. bracteate. The qRT-PCR was used to analyze the temporal and spatial expression patterns of these genes in various tissues of A. bracteata under drought stress, and to analyze their response to drought stress. The results showed that a total of 55 MYB-related family members of A. bracteata were identified and divided into 5 subgroups. The encoded proteins were mostly hydrophilic and mainly located in the nucleus. Motif 2 was the characteristic motif, while Motif 4 had the highest conservation. The secondary and tertiary structures of their proteins were mainly irregularly coiled. Expression analysis showed that after 24 h of drought stress, gene expression levels were higher than those at 6 h and 0 h, and most of these genes were highly expressed in the leaves. These genes responded more strongly in roots and stems under severe drought, while they had higher expression levels in leaves under mild drought. In summary, the MYB-related gene family of A. bracteata exhibited distinct tissue-specific expression patterns under drought stress, laying a theoretical foundation for subsequent functional research and stress resistant breeding.
    Atraphaxis bracteata  /  MYB-related  /  Drought stress  /  Expression pattern
    何彩, 刘源, 晋敏, 吴媛, 王素桢, 胡芳. 沙木蓼MYB-related基因家族的鉴定及其响应干旱胁迫的表达模式研究. 草地学报, 2026 , 34 (6) : 2012 -2021 . DOI: 10.11733/j.issn.1007-0435.2026.06.005
    HE Cai, LIU Yuan, JIN Min, WU Yuan, WANG Su-zhen, HU Fang. Identification of MYB-related Gene Family in Atraphaxis bracteata and Study on its Expression Pattern in Response to Drought Stress[J]. Acta Agrestia Sinica, 2026 , 34 (6) : 2012 -2021 . DOI: 10.11733/j.issn.1007-0435.2026.06.005

      甘肃省科技计划项目(自然科学基金) (24JRRH007,25JRRH004); 武威市科技创新人才计划项目 (WW25A01RYFL002)

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    doi: 10.11733/j.issn.1007-0435.2026.06.005
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    小菇属 Mycena 11 5.26
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