Article(id=1309575023573172892, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.08.028, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1748620800000, receivedDateStr=2025-05-31, revisedDate=1764000000000, revisedDateStr=2025-11-25, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156616347, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156616347, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156616347, creator=13701087609, updateTime=1790156616347, updator=13701087609, issue=Issue{id=1309574965775659485, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='8', pageStart='2753', pageEnd='3140', issueExtLink='null', onlineDate='null', pubDate='1786723200000', pubDateStr='2026-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156602567, creator='13701087609', updateTime=1790156935415, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576361992352087, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576361992352088, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3060, endPage=3069, ext={EN=ArticleExt(id=1309575024055517854, articleId=1309575023573172892, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Population Genetic Structure and Historical Dynamics of Xanthopappus subacaulis Based on Single Copy Nuclear Genes, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=In order to investigate the genetic structure and historical dynamics of natural populations of Xanthopappus subacaulis, we sequenced the DNAs of 147 individuals from 49 populations based on ten single-copy nuclear genes. Multiple methods, including STRUCTURE clustering and molecular variance analysis, were employed for analysis. The results indicated that the polymorphic loci of X. subacaulis ranged from 3 to 11, with haplotype diversity index (Hd) ranging from 0.558 to 0.883, nucleotide diversity index (π) from 0.001 32 to 0.006 03, and mean nucleotide differences (K) from 0.738 to 3.325, indicating rich genetic diversity. The tested populations of X. subacaulis could be divided into two major genealogical branches in the north and south, with 83.04% of the genetic variation among populations and only 16.96% within populations. The degree of genetic differentiation was high (FST=0.830, P<0.01), and gene flow was extremely low (Nm=0.112). The genetic distance was significantly positively correlated with the geographical distance (r=0.537,P<0.01). The divergence time of the two major genealogical branches was approximately 3.33 million years ago, which was related to the uplift of the Qinghai-Xizang Plateau and the climate change during the Quaternary glacial period. Moreover, there was no recent population expansion. This study revealed the influence of geographical isolation, climate differences, and historical events on the genetic structure of X. subacaulis, which provided important data for its conservation of germplasm resources and adaptive evolution research., authors=ZHENG Ying-hui1,2, SU Xu1,2,3, LIU Yu-ping1,2,3, CAIRANG Zha-xi1,2, LEI Jie-qiong1,2, WEI Kai-yue1,2, FENG Xu1,2, YANG Ping1, authorsList=ZHENG Ying-hui, SU Xu, LIU Yu-ping, CAIRANG Zha-xi, LEI Jie-qiong, WEI Kai-yue, FENG Xu, YANG Ping, authorCompany=1 School of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China; 2 Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Xizang Plateau in Qinghai Province, Qinghai Normal University, Xining, Qinghai Province 810008, China; 3 Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, Qinghai Province 810016, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575023984214685, articleId=1309575023573172892, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=基于单拷贝核基因的黄缨菊群体遗传结构与历史动态研究, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为探究黄缨菊(Xanthopappus subacaulis)自然居群的遗传结构与历史动态,本研究基于10个单拷贝核基因数据对其49个居群147个个体进行DNA测序,利用STRUCTURE聚类、分子方差分析等多种方法进行解析。结果显示,黄缨菊多态性位点为3~11,单倍型多样性指数(Hd)为0.558~0.883,核苷酸多样性指数(π)为0.001 32~0.006 03,平均核苷酸差异数(K)为0.738~3.325,遗传多样性丰富;黄缨菊参试居群可被划分为南北两大谱系分支,居群间遗传变异占比83.04%,居群内占16.96%,遗传分化程度较高(FST=0.830,P<0.01),基因流极低(Nm=0.112),遗传距离与地理距离呈显著正相关(r=0.537,P<0.01)。两大谱系分化时间约为3.33 Mya,与青藏高原隆升及第四纪冰期气候变化相关,近期无种群扩张。本研究揭示了地理隔离、气候差异和历史事件对黄缨菊群体遗传结构的影响,为其种质资源保护及适应性进化研究提供重要数据基础。, authors=郑莹晖1,2, 苏旭1,2,3, 刘玉萍1,2,3, 才让扎西1,2, 雷洁琼1,2, 韦凯悦1,2, 冯旭1,2, 杨萍1, authorsList=郑莹晖, 苏旭, 刘玉萍, 才让扎西, 雷洁琼, 韦凯悦, 冯旭, 杨萍, authorCompany=1 青海师范大学生命科学学院, 青海 西宁 810008; 2 青海师范大学青海省青藏高原生物多样性形成机制与综合利用重点实验室, 青海 西宁 810008; 3 青海师范大学高原科学与可持续发展研究院, 青海 西宁 810016, correspAuthors=null, authorNote=郑莹晖(1999-),女,汉族,四川绵阳人,硕士研究生,主要从事高山植物系统与进化研究,E-mail:yhzheng99@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=CvQ1h5ar1BHc0ot8yqlf/g==, pdfFileSize=5056261, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=青海省自然科学基金面上项目 (2025-ZJ-996M); 国家自然科学基金项目 (32160297))}, authors=null, keywords=[Keyword(id=1309575024332341919, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575023573172892, language=CN, orderNo=1, keyword=单拷贝核基因), Keyword(id=1309575024395256480, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575023573172892, language=CN, orderNo=2, keyword=黄缨菊), Keyword(id=1309575024449782433, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575023573172892, language=CN, orderNo=3, keyword=遗传多样性), Keyword(id=1309575024516891298, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575023573172892, language=CN, orderNo=4, keyword=遗传结构), Keyword(id=1309575024596583075, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309575023573172892, language=CN, orderNo=5, keyword=地理隔离), Keyword(id=1309575024781132452, 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The shifting balance theory and macroevolution[J]. Annual Review of Genetics,1982,16:1-19 MORJAN C L,RIESEBERG L H. How species evolve collectively: implications of gene flow and selection for the spread of advantageous alleles[J]. Molecular Ecology,2004,13(6):1341-1356 WANG Y S,HUANG H W,WANG Y. Recent progress in plant molecular population genetics[J]. Hereditas,2007,29(10):1191-1198王云生,黄宏文,王瑛. 植物分子群体遗传学研究动态[J]. 遗传,2007,29(10):1191-1198 LIU M Y,WANG T J,XIU F,et al. Phylogeography and genetic structure of Artemisia halodendron based on single copy nuclear genes[J]. Acta Agrestia Sinica,2024,32(10):3034-3042刘梦瑶,王铁娟,秀芳,等. 基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究[J]. 草地学报,2024,32(10):3034-3042 JIA Y Y,QIU Y P,ZHOU X Y,et al. Genetic diversity analysis of Clematis macropetala based on morphological markers and ISSR molecular markers[J]. Acta Agrestia Sinica,2024,32(1):75-86贾艳艳,邱玉鹏,周欣莹,等. 基于形态标记和ISSR分子标记的长瓣铁线莲遗传多样性分析[J]. 草地学报,2024,32(1):75-86 XU Z N,WANG X,WANG Q,et al. Karyotype analysis of 13 wild populations of Pistacia chinensis[J]. Molecular Plant Breeding,2025,23(5):1564-1573许泽楠,王鑫,王琦,等. 13个黄连木野生居群的染色体核型分析[J]. 分子植物育种,2025,23(5):1564-1573 XIA H N,LI D X,ZHOU J K,et al. Genetic diversity analysis of mononuclear strains of Pleurotus ostreatus and Pleurotus ostreatus with white variation[J]. Jiangsu Agricultural Sciences,2024,52(7):41-47夏会楠,李东晓,周金看,等. 黑平菇及白色变异平菇单核菌株的遗传多样性分析[J]. 江苏农业科学,2024,52(7):41-47 LIU C H,DONG Y N,HAN Y L,et al. Research and application of molecular markers in millet[J]. Journal of Food Safety & Quality,2021,12(3):1002-1008刘程宏,董亚南,韩燕丽,等. 谷子分子标记研究与应用[J]. 食品安全质量检测学报,2021,12(3):1002-1008 GROVER A,SHARMA P C. Development and use of molecular markers: past and present[J]. Critical Reviews in Biotechnology,2016,36(2):290-302 QIN A L,WANG M M,CUN Y Z,et al. Phylogeographic evidence for a link of species divergence of Ephedra in the Qinghai-Tibetan Plateau and adjacent regions to the Miocene Asian aridification[J]. PLoS One,2013,8(2):e56243 TANG S R,WANG T J,XIU F,et al. Genetic structure and phylogeography analysis of Artemisia intramongolica based on single copy nuclear genes[J]. Bulletin of Botanical Research,2022,42(6):956-963唐思如,王铁娟,秀芳,等. 基于单拷贝核基因的褐沙蒿遗传结构和谱系地理学分析[J]. 植物研究,2022,42(6):956-963 CHEN Q,SONG J,DU W P,et al. Phylogenetic analyses of four Chinese endemic wheat landraces based on two single copy genes[J]. Cereal Research Communications,2018,46(2):191-200 XIU F,WANG T J,TANG S R,et al. Study on the genetic structure and phylogeography of Artemisia ordosica[J]. Chinese Journal of Grassland,2025,47(1):96-104秀芳,王铁娟,唐思如,等. 黑沙蒿的遗传结构与谱系地理学研究[J]. 中国草地学报,2025,47(1):96-104 LI S,LIU S L,PEI S Y,et al. A study on genetic diversity of Camellia huana by single-copy nuclear gene PAL[J]. Journal of Guangxi Normal University(Natural Science Edition),2021,39(1):128-135李爽,刘上丽,裴思玉,等. 基于单拷贝核基因PAL研究贵州金花茶遗传多样性[J]. 广西师范大学学报(自然科学版),2021,39(1):128-135 SHI Z. Flora of China[M]. 1st ed. Beijing: Science Publishing House,1980:61石铸. 中国植物志[M]. 第1版. 北京:科学出版社,1987:61 LIU S W. Flora of Qinghai- Volume 3[M]. Xining: Qinghai People’s Publishing House,1996:437刘尚武. 青海植物志-第三卷[M]. 西宁:青海人民出版社,1996:437 ZHAO R N. Compendium of Chinese Medicinal Herb Resources in Gansu[M]. 1st ed. Lanzhou: Gansu Science and Technology Publishing House,2007:480赵汝能. 甘肃中草药资源志[M]. 第1版. 兰州:甘肃科学技术出版社,2007:480 ZHANG L,CHEN C J,CHEN J,et al. Thiophene acetylenes and furanosesquiterpenes from Xanthopappus subacaulis and their antibacterial activities[J]. Phytochemistry,2014,106:134-140 JIN J R,LIU Y P,SU X,et al. Chromosome karyotype studies on different populations of Xanthopappus subacaulis(Asteraceae), an endemic species from the Qinghai-Xizang Plateau[J]. Acta Agrestia Sinica,2025,33(1):53-61靳佳瑞,刘玉萍,苏旭,等. 青藏高原特有种—黄缨菊不同居群的染色体核型研究[J]. 草地学报,2025,33(1):53-61 SHANG J N. Studies on the chemical constituents of Xanthopappus subacaulis C.Winkl. and Clerodendrum japonicum (Thunb.) Sweet[D]. Lanzhou:Lanzhou University,2010:23-30尚冀宁. 黄缨菊和赪桐化学成分研究[D]. 兰州:兰州大学,2010:23-30 MA Z L. Phylogeography of Xanthopappus subacaulis(Asteraceae), an endemic species from the Qinghai-Tibet Plateau[D]. Xining:Qinghai Normal University,2022:21-45马子兰. 青藏高原特有种黄缨菊谱系地理学研究[D]. 西宁:青海师范大学,2022:21-45 TAMURA K,STECHER G,PETERSON D,et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution,2013,30(12):2725-2729 ZHANG D,GAO F L,JAKOVLIĆ I,et al. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies[J]. Molecular Ecology Resources,2020,20(1):348-355 LIBRADO P,ROZAS J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11):1451-1452 HUBISZ M J,FALUSH D,STEPHENS M,et al. Inferring weak population structure with the assistance of sample group information[J]. Molecular Ecology Resources,2009,9(5):1322-1332 EARL D A,VONHOLDT B M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method[J]. Conservation Genetics Resources,2012,4(2):359-361 PEAKALL R,SMOUSE P E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update[J]. Bioinformatics,2012,28(19):2537-2539 EXCOFFIER L,LISCHER H E L. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources,2010,10(3):564-567 WRIGHT S. Evolution in mendelian populations[J]. Bulletin of Mathematical Biology,1990,52(1):241-295 LÜ T. Studies on genetic diversity of Psammochloa villosa, apeculiar sandy plant[D]. Xining:Qinghai Normal University,2019:53吕婷. 沙生牧草沙鞭的遗传多样性研究[D]. 西宁:青海师范大学,2019:53 MU J J,ZHOU X,MU H Y,et al. Mantel test analysis of quality characteristics and main physicochemical properties of sweet potato coarse cereals powder[J]. Cereals & Oils,2024,37(9):31-34,40穆晶晶,周翔,母浩圆,等. 甘薯杂粮粉品质特征与主要理化性质的Mantel test分析[J]. 粮食与油脂,2024,37(9):31-34,40 BANDELT H J,FORSTER P,RÖHL A. Median-joining networks for inferring intraspecific phylogenies[J]. Molecular Biology and Evolution,1999,16(1):37-48 TAJIMA F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism[J]. Genetics,1989,123(3):585-595 FU Y X,LI W H. Statistical tests of neutrality of mutations[J]. Genetics,1993,133(3):693-709 NEI M,HARTL D L. The new mutation theory of phenotypic evolution[J]. Proceedings of the National Academy of Sciences of the United States of America,2007,104(30):12235-12242 SUN Y Q. Genetic diversity and genetic basis of local adaptation in Pinus yunnanensis[D]. Beijing:Beijing Forestry University,2020:26-37孙彦强. 云南松遗传变异格局及适应性分化的遗传基础研究[D]. 北京:北京林业大学,2020:26-37 HUNTER N. Meiotic recombination: the essence of heredity[J]. Cold Spring Harbor Perspectives in Biology,2015,7(12):a016618 KAMEYAMA Y,ISAGI Y,NAITO K,et al. Microsatellite analysis of pollen flow in Rhododendron metternichii var. hondoense[J]. Ecological Research,2000,15(3):263-269 DU M F,DING G J. Analysis of genetic structure and its related factors of Pinus massoniana from different populations by ISSR marker[J]. Guihaia,2016,36(9):1068-1075杜明凤,丁贵杰. 不同种源马尾松ISSR遗传结构及影响因素分析[J]. 广西植物,2016,36(9):1068-1075 ZHANG X L. Influence of climatic factors on genetic diversity and genetic structure of Cleistogenes squarrosa Keng at large scale gradient[D]. Shenyang:Liaoning University,2023:17-60张雪丽. 大尺度梯度下气候因素对糙隐子草遗传多样性和遗传结构的影响[D]. 沈阳:辽宁大学,2023:17-60 DYNESIUS M,JANSSON R. Evolutionary consequences of changes in species’ geographical distributions driven by Milankovitch climate oscillations[J]. PNAS,2000,97(16):9115-9120 HEWITT G M. Genetic consequences of climatic oscillations in the Quaternary[J]. Philosophical Transactions of the Royal Society of London Series B,Biological Sciences,2004,359(1442):183-195 QIU Y X,FU C X,COMES H P. Plant molecular phylogeography in China and adjacent regions: tracing the genetic imprints of Quaternary climate and environmental change in the world’s most diverse temperate flora[J]. Molecular Phylogenetics and Evolution,2011,59(1):225-244 GAO Q B,ZHANG F Q,XING R,et al. Phylogeographic study revealed microrefugia for an endemic species on the Qinghai-Tibetan Plateau: Rhodiola chrysanthemifolia (Crassulaceae)[J]. Plant Systematics and Evolution,2016,302(9):1179-1193)
Population Genetic Structure and Historical Dynamics of Xanthopappus subacaulis Based on Single Copy Nuclear Genes
ZHENG Ying-hui1,2, SU Xu1,2,3, LIU Yu-ping1,2,3, CAIRANG Zha-xi1,2, LEI Jie-qiong1,2, WEI Kai-yue1,2, FENG Xu1,2, YANG Ping1
Affiliations
1 School of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China; 2 Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Xizang Plateau in Qinghai Province, Qinghai Normal University, Xining, Qinghai Province 810008, China; 3 Academy of Plateau Science and Sustainability, Qinghai Normal University, Xining, Qinghai Province 810016, China
In order to investigate the genetic structure and historical dynamics of natural populations of Xanthopappus subacaulis, we sequenced the DNAs of 147 individuals from 49 populations based on ten single-copy nuclear genes. Multiple methods, including STRUCTURE clustering and molecular variance analysis, were employed for analysis. The results indicated that the polymorphic loci of X. subacaulis ranged from 3 to 11, with haplotype diversity index (Hd) ranging from 0.558 to 0.883, nucleotide diversity index (π) from 0.001 32 to 0.006 03, and mean nucleotide differences (K) from 0.738 to 3.325, indicating rich genetic diversity. The tested populations of X. subacaulis could be divided into two major genealogical branches in the north and south, with 83.04% of the genetic variation among populations and only 16.96% within populations. The degree of genetic differentiation was high (FST=0.830, P<0.01), and gene flow was extremely low (Nm=0.112). The genetic distance was significantly positively correlated with the geographical distance (r=0.537,P<0.01). The divergence time of the two major genealogical branches was approximately 3.33 million years ago, which was related to the uplift of the Qinghai-Xizang Plateau and the climate change during the Quaternary glacial period. Moreover, there was no recent population expansion. This study revealed the influence of geographical isolation, climate differences, and historical events on the genetic structure of X. subacaulis, which provided important data for its conservation of germplasm resources and adaptive evolution research.
ZHENG Ying-hui, SU Xu, LIU Yu-ping, CAIRANG Zha-xi, LEI Jie-qiong, WEI Kai-yue, FENG Xu, YANG Ping.
Population Genetic Structure and Historical Dynamics of Xanthopappus subacaulis Based on Single Copy Nuclear Genes[J].
Acta Agrestia Sinica,
2026
, 34
(8)
: 3060
-3069
.
DOI: 10.11733/j.issn.1007-0435.2026.08.028
WRIGHT S. The shifting balance theory and macroevolution[J]. Annual Review of Genetics,1982,16:1-19 MORJAN C L,RIESEBERG L H. How species evolve collectively: implications of gene flow and selection for the spread of advantageous alleles[J]. Molecular Ecology,2004,13(6):1341-1356 WANG Y S,HUANG H W,WANG Y. Recent progress in plant molecular population genetics[J]. Hereditas,2007,29(10):1191-1198王云生,黄宏文,王瑛. 植物分子群体遗传学研究动态[J]. 遗传,2007,29(10):1191-1198 LIU M Y,WANG T J,XIU F,et al. Phylogeography and genetic structure of Artemisia halodendron based on single copy nuclear genes[J]. Acta Agrestia Sinica,2024,32(10):3034-3042刘梦瑶,王铁娟,秀芳,等. 基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究[J]. 草地学报,2024,32(10):3034-3042 JIA Y Y,QIU Y P,ZHOU X Y,et al. Genetic diversity analysis of Clematis macropetala based on morphological markers and ISSR molecular markers[J]. Acta Agrestia Sinica,2024,32(1):75-86贾艳艳,邱玉鹏,周欣莹,等. 基于形态标记和ISSR分子标记的长瓣铁线莲遗传多样性分析[J]. 草地学报,2024,32(1):75-86 XU Z N,WANG X,WANG Q,et al. Karyotype analysis of 13 wild populations of Pistacia chinensis[J]. Molecular Plant Breeding,2025,23(5):1564-1573许泽楠,王鑫,王琦,等. 13个黄连木野生居群的染色体核型分析[J]. 分子植物育种,2025,23(5):1564-1573 XIA H N,LI D X,ZHOU J K,et al. Genetic diversity analysis of mononuclear strains of Pleurotus ostreatus and Pleurotus ostreatus with white variation[J]. Jiangsu Agricultural Sciences,2024,52(7):41-47夏会楠,李东晓,周金看,等. 黑平菇及白色变异平菇单核菌株的遗传多样性分析[J]. 江苏农业科学,2024,52(7):41-47 LIU C H,DONG Y N,HAN Y L,et al. Research and application of molecular markers in millet[J]. Journal of Food Safety & Quality,2021,12(3):1002-1008刘程宏,董亚南,韩燕丽,等. 谷子分子标记研究与应用[J]. 食品安全质量检测学报,2021,12(3):1002-1008 GROVER A,SHARMA P C. Development and use of molecular markers: past and present[J]. Critical Reviews in Biotechnology,2016,36(2):290-302 QIN A L,WANG M M,CUN Y Z,et al. Phylogeographic evidence for a link of species divergence of Ephedra in the Qinghai-Tibetan Plateau and adjacent regions to the Miocene Asian aridification[J]. PLoS One,2013,8(2):e56243 TANG S R,WANG T J,XIU F,et al. Genetic structure and phylogeography analysis of Artemisia intramongolica based on single copy nuclear genes[J]. Bulletin of Botanical Research,2022,42(6):956-963唐思如,王铁娟,秀芳,等. 基于单拷贝核基因的褐沙蒿遗传结构和谱系地理学分析[J]. 植物研究,2022,42(6):956-963 CHEN Q,SONG J,DU W P,et al. Phylogenetic analyses of four Chinese endemic wheat landraces based on two single copy genes[J]. Cereal Research Communications,2018,46(2):191-200 XIU F,WANG T J,TANG S R,et al. Study on the genetic structure and phylogeography of Artemisia ordosica[J]. Chinese Journal of Grassland,2025,47(1):96-104秀芳,王铁娟,唐思如,等. 黑沙蒿的遗传结构与谱系地理学研究[J]. 中国草地学报,2025,47(1):96-104 LI S,LIU S L,PEI S Y,et al. A study on genetic diversity of Camellia huana by single-copy nuclear gene PAL[J]. Journal of Guangxi Normal University(Natural Science Edition),2021,39(1):128-135李爽,刘上丽,裴思玉,等. 基于单拷贝核基因PAL研究贵州金花茶遗传多样性[J]. 广西师范大学学报(自然科学版),2021,39(1):128-135 SHI Z. Flora of China[M]. 1st ed. Beijing: Science Publishing House,1980:61石铸. 中国植物志[M]. 第1版. 北京:科学出版社,1987:61 LIU S W. Flora of Qinghai- Volume 3[M]. Xining: Qinghai People’s Publishing House,1996:437刘尚武. 青海植物志-第三卷[M]. 西宁:青海人民出版社,1996:437 ZHAO R N. Compendium of Chinese Medicinal Herb Resources in Gansu[M]. 1st ed. Lanzhou: Gansu Science and Technology Publishing House,2007:480赵汝能. 甘肃中草药资源志[M]. 第1版. 兰州:甘肃科学技术出版社,2007:480 ZHANG L,CHEN C J,CHEN J,et al. Thiophene acetylenes and furanosesquiterpenes from Xanthopappus subacaulis and their antibacterial activities[J]. Phytochemistry,2014,106:134-140 JIN J R,LIU Y P,SU X,et al. Chromosome karyotype studies on different populations of Xanthopappus subacaulis(Asteraceae), an endemic species from the Qinghai-Xizang Plateau[J]. Acta Agrestia Sinica,2025,33(1):53-61靳佳瑞,刘玉萍,苏旭,等. 青藏高原特有种—黄缨菊不同居群的染色体核型研究[J]. 草地学报,2025,33(1):53-61 SHANG J N. Studies on the chemical constituents of Xanthopappus subacaulis C.Winkl. and Clerodendrum japonicum (Thunb.) Sweet[D]. Lanzhou:Lanzhou University,2010:23-30尚冀宁. 黄缨菊和赪桐化学成分研究[D]. 兰州:兰州大学,2010:23-30 MA Z L. Phylogeography of Xanthopappus subacaulis(Asteraceae), an endemic species from the Qinghai-Tibet Plateau[D]. Xining:Qinghai Normal University,2022:21-45马子兰. 青藏高原特有种黄缨菊谱系地理学研究[D]. 西宁:青海师范大学,2022:21-45 TAMURA K,STECHER G,PETERSON D,et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution,2013,30(12):2725-2729 ZHANG D,GAO F L,JAKOVLIĆ I,et al. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies[J]. Molecular Ecology Resources,2020,20(1):348-355 LIBRADO P,ROZAS J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11):1451-1452 HUBISZ M J,FALUSH D,STEPHENS M,et al. Inferring weak population structure with the assistance of sample group information[J]. Molecular Ecology Resources,2009,9(5):1322-1332 EARL D A,VONHOLDT B M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method[J]. Conservation Genetics Resources,2012,4(2):359-361 PEAKALL R,SMOUSE P E. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update[J]. Bioinformatics,2012,28(19):2537-2539 EXCOFFIER L,LISCHER H E L. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources,2010,10(3):564-567 WRIGHT S. Evolution in mendelian populations[J]. Bulletin of Mathematical Biology,1990,52(1):241-295 LÜ T. Studies on genetic diversity of Psammochloa villosa, apeculiar sandy plant[D]. Xining:Qinghai Normal University,2019:53吕婷. 沙生牧草沙鞭的遗传多样性研究[D]. 西宁:青海师范大学,2019:53 MU J J,ZHOU X,MU H Y,et al. Mantel test analysis of quality characteristics and main physicochemical properties of sweet potato coarse cereals powder[J]. Cereals & Oils,2024,37(9):31-34,40穆晶晶,周翔,母浩圆,等. 甘薯杂粮粉品质特征与主要理化性质的Mantel test分析[J]. 粮食与油脂,2024,37(9):31-34,40 BANDELT H J,FORSTER P,RÖHL A. Median-joining networks for inferring intraspecific phylogenies[J]. Molecular Biology and Evolution,1999,16(1):37-48 TAJIMA F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism[J]. Genetics,1989,123(3):585-595 FU Y X,LI W H. Statistical tests of neutrality of mutations[J]. Genetics,1993,133(3):693-709 NEI M,HARTL D L. The new mutation theory of phenotypic evolution[J]. Proceedings of the National Academy of Sciences of the United States of America,2007,104(30):12235-12242 SUN Y Q. Genetic diversity and genetic basis of local adaptation in Pinus yunnanensis[D]. Beijing:Beijing Forestry University,2020:26-37孙彦强. 云南松遗传变异格局及适应性分化的遗传基础研究[D]. 北京:北京林业大学,2020:26-37 HUNTER N. Meiotic recombination: the essence of heredity[J]. Cold Spring Harbor Perspectives in Biology,2015,7(12):a016618 KAMEYAMA Y,ISAGI Y,NAITO K,et al. Microsatellite analysis of pollen flow in Rhododendron metternichii var. hondoense[J]. Ecological Research,2000,15(3):263-269 DU M F,DING G J. Analysis of genetic structure and its related factors of Pinus massoniana from different populations by ISSR marker[J]. Guihaia,2016,36(9):1068-1075杜明凤,丁贵杰. 不同种源马尾松ISSR遗传结构及影响因素分析[J]. 广西植物,2016,36(9):1068-1075 ZHANG X L. Influence of climatic factors on genetic diversity and genetic structure of Cleistogenes squarrosa Keng at large scale gradient[D]. Shenyang:Liaoning University,2023:17-60张雪丽. 大尺度梯度下气候因素对糙隐子草遗传多样性和遗传结构的影响[D]. 沈阳:辽宁大学,2023:17-60 DYNESIUS M,JANSSON R. Evolutionary consequences of changes in species’ geographical distributions driven by Milankovitch climate oscillations[J]. PNAS,2000,97(16):9115-9120 HEWITT G M. Genetic consequences of climatic oscillations in the Quaternary[J]. Philosophical Transactions of the Royal Society of London Series B,Biological Sciences,2004,359(1442):183-195 QIU Y X,FU C X,COMES H P. Plant molecular phylogeography in China and adjacent regions: tracing the genetic imprints of Quaternary climate and environmental change in the world’s most diverse temperate flora[J]. Molecular Phylogenetics and Evolution,2011,59(1):225-244 GAO Q B,ZHANG F Q,XING R,et al. Phylogeographic study revealed microrefugia for an endemic species on the Qinghai-Tibetan Plateau: Rhodiola chrysanthemifolia (Crassulaceae)[J]. Plant Systematics and Evolution,2016,302(9):1179-1193