Article(id=1309575013846581885, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.08.035, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1763308800000, receivedDateStr=2025-11-17, revisedDate=1765814400000, revisedDateStr=2025-12-16, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156614027, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156614027, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156614027, creator=13701087609, updateTime=1790156614027, updator=13701087609, issue=Issue{id=1309574965775659485, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='8', pageStart='2753', pageEnd='3140', issueExtLink='null', onlineDate='null', pubDate='1786723200000', pubDateStr='2026-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156602567, creator='13701087609', updateTime=1790156935415, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576361992352087, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576361992352088, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3131, endPage=3140, ext={EN=ArticleExt(id=1309575014773523071, articleId=1309575013846581885, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation, Antioxidant and Digestibility of Alfalfa Baled Silage, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=This study investigated the effects of inoculation with feruloyl esterase-producing lactic acid bacteria Lactobacillus reuteri A4-2 on the fermentation quality, antioxidant status, and in vitro digestibility of baled alfalfa silage. Chopped fresh alfalfa (Medicago sativa L.) at the early to full-blossom flowering stage was randomly divided into three groups: CON (without inoculant), YM13 (inoculated with non-feruloyl esterase-producing L. reuteri YM13), and A4-2 (inoculated with L. reuteri A4-2). Samples were collected after 20, 40, and 60 d of ensiling to determine fermentation characteristics, free ferulic acid content, antioxidant enzyme activities, chemical composition, and in vitro dry matter digestibility. Compared with the CON and YM13, inoculation with L. reuteri A4-2 significantly reduced pH but increased lactic and acetic acid contents (P<0.05). Both inoculating L. reuteri YM13 and L. reuteri A4-2 in silage showed a higher dry matter content and lower ammonia nitrogen/total nitrogen than CON (P<0.05). Inoculation with L. reuteri A4-2 in alfalfa silage markedly enhanced the levels of free ferulic acid, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) activities, and total antioxidant capacity (T-AOC) (P<0.05) relative to CON and YM13 during ensiling. In addition, inoculation with L. reuteri A4-2 effectively decreased neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents, thereby improving in vitro DM digestibility compared with the other treatments (P<0.05). Overall, inoculation with L. reuteri A4-2 not only improved the fermentation quality of silage, but also enhanced the antioxidant capacity, reduced its fiber content, and improved its in vitro DM digestibility compared with the CON and YM13 groups, demonstrating significant application advantages., authors=ZHANG Yan-wei1, MAO Yu-qing1, WANG Peng1, TAO You-yan1, GUO Xu-sheng2, LI Fu-hou1, authorsList=ZHANG Yan-wei, MAO Yu-qing, WANG Peng, TAO You-yan, GUO Xu-sheng, LI Fu-hou, authorCompany=1 College of Animal Sciences, Jilin University, Changchun, Jilin Province 130062, China; 2 College of Life Sciences, Lanzhou University, Lanzhou, Gansu Province 730000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309575014693831294, articleId=1309575013846581885, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=接种产阿魏酸酯酶罗伊氏乳杆菌对苜蓿裹包青贮发酵、抗氧化及消化率的影响, columnId=1309565376569114713, journalTitle=草地学报, columnName=技术研发, runingTitle=null, highlight=null, articleAbstract=为探讨接种产阿魏酸酯酶的罗伊氏乳杆菌A4-2(Lactobacillus reuteri A4-2)对裹包苜蓿青贮饲料发酵品质、抗氧化性能及体外消化率的影响,本试验以切碎的初花期至盛花期紫花苜蓿(Medicago sativa L.)为原料,随机分为3组进行裹包青贮:CON组(不接种乳酸菌)、YM13组(接种不产阿魏酸酯酶的L. reuteri YM13)和A4-2组(接种产阿魏酸酯酶的L.reuteri A4-2)。青贮至20,40和60 d时测定其发酵特性、游离阿魏酸含量、抗氧化酶活性、化学成分及体外干物质消化率。结果表明:与CON和YM13组相比,接种L. reuteri A4-2能显著降低青贮饲料的pH值并提高乳酸和乙酸含量(P<0.05)。接种L. reuteri YM13和L. reuteri A4-2均较CON提高了青贮饲料中干物质含量,并降低了氨态氮/总氮(P<0.05)。A4-2处理组的游离阿魏酸含量、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GPX)活性及总抗氧化能力(Total antioxidant capacity,T-AOC)均显著高于其他两组(P<0.05)。此外,接种L. reuteri A4-2显著降低了青贮饲料的中性洗涤纤维(Neutral detergent fiber,NDF)和酸性洗涤纤维(Acid detergent fiber,ADF)含量,并最终使其体外干物质消化率显著高于其他两组(P<0.05)。综上,接种L. reuteri A4-2不仅可改善裹包苜蓿青贮的发酵品质,而且相较于CON和YM13处理组,还能增强青贮饲料的抗氧化能力,降低纤维含量并提高消化率,具有显著的应用优势。, authors=张艳威1, 毛玉青1, 王鹏1, 陶有艳1, 郭旭生2, 李福厚1, authorsList=张艳威, 毛玉青, 王鹏, 陶有艳, 郭旭生, 李福厚, authorCompany=1 吉林大学动物科学学院, 吉林 长春 130062; 2 兰州大学生命科学学院, 甘肃 兰州 730000, correspAuthors=null, authorNote=张艳威(2002-),男,汉族,山东聊城人,硕士研究生,主要从事饲草生产加工与反刍动物健康研究,E-mail:19861208291@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=U0DAuu2EGRQmoi6OI7uJ2A==, pdfFileSize=2822388, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32301493))}, authors=null, keywords=[Keyword(id=1309575016111506048, 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Impact of oxidative stress on the health and immune function of dairy cattle[J]. Veterinary Immunology and Immunopathology,2009,128(1-3):104-109 CASTILLO C,PEREIRA V,ABUELO Á,et al. Effect of supplementation with antioxidants on the quality of bovine milk and meat production[J]. The Scientific World Journal,2013,2013:616098 ABUELO A,HERNÁNDEZ J,BENEDITO J L,et al. The importance of the oxidative status of dairy cattle in the periparturient period: revisiting antioxidant supplementation[J]. Journal of Animal Physiology and Animal Nutrition,2015,99(6):1003-1016 LOURENÇO S C,MOLDÃO-MARTINS M,ALVES V D. Antioxidants of natural plant origins: From sources to food industry applications[J]. Molecules,2019,24(22):4132 WANG T,LI J,LU Q,et al. Effects of total flavonoids from Taraxacum mongolicum and Bacillus subtilis on fermentation quality, antioxidant activity, and aerobic stability of Avena sativa silage[J]. Acta Prataculturae Sinica,2025,35(6):1-14王涛,李静,卢强,等. 蒲公英黄酮和枯草芽孢杆菌对燕麦青贮品质、抗氧化活性及微生物群落结构的影响[J]. 草业学报,2025,35(6):1-14 HUO W J,ZHANG Y J,ZHANG L Y,et al. Effect of lactobacilli inoculation on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage[J]. Frontiers in Microbiology,2022,13:1070175 HORVAT D,VILJEVAC VULETIĆ M,ANDRIĆ L,et al. Characterization of forage quality, phenolic profiles, and antioxidant activity in alfalfa (Medicago sativa L.)[J]. Plants,2022,11(20):2735 BHANDARI K B,RUSCH H L,HEUSCHELE D J. Alfalfa stem cell wall digestibility: Current knowledge and future research directions[J]. Agronomy,2023,13(12):2875 KIM D,LEE K D,CHOI K C. Role of LAB in silage fermentation: Effect on nutritional quality and organic acid production-An overview[J]. Aims Agriculture and Food,2021,6(1):216-234 ZHANG Y X,USMAN S,LI Q,et al. Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats[J]. Journal of Animal Science and Biotechnology,2024,15(1):9 LI F H,ZHANG B B,ZHANG Y X,et al. Probiotic effect of ferulic acid esterase-producing Lactobacillus plantarum inoculated alfalfa silage on digestion, antioxidant, and immunity status of lactating dairy goats[J]. Animal Nutrition,2022,11:38-47 KHARAZIAN Z A,JIA M Y,SU R N,et al. Fermentation quality, chemical composition, and antioxidant capacity of alfalfa and corn silages inoculated with exopolysaccharide-producing lactic acid bacteria[J]. Animal Feed Science and Technology,2025,329:116490 LI F H,DING Z T,CHEN X Z,et al. The effects of Lactobacillus plantarum with feruloyl esterase-producing ability or high antioxidant activity on the fermentation, chemical composition, and antioxidant status of alfalfa silage[J]. Animal Feed Science and Technology,2021,273:114835 DONAGHY J,KELLY P F,MCKAY A M. Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli[J]. Applied Microbiology and Biotechnology,1998,50(2):257-260 LI F H,DING Z T,KE W C,et al. Ferulic acid esterase-producing lactic acid bacteria and cellulase pretreatments of corn stalk silage at two different temperatures: Ensiling characteristics, carbohydrates composition and enzymatic saccharification[J]. Bioresource Technology,2019,282:211-221 KE W C,DING W R,XU D M,et al. Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage[J]. Journal of Dairy Science,2017,100(11):8958-8966 VAN SOEST P J,ROBERTSON J B,LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597 ARTHUR THOMAS T. An automated procedure for the determination of soluble carbohydrates in herbage[J]. Journal of the Science of Food and Agriculture,1977,28(7):639-642 THEODOROU M K,WILLIAMS B A,DHANOA M S,et al. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds[J]. Animal Feed Science and Technology,1994,48(3-4):185-197 WU C R,DAI S,LIANG L F,et al.Effects of different additives on fermentation quality and protein degradation of Broussonetia papyrifera silage[J]. Acta Prataculturae Sinica,2021,30(10):169-179吴长荣,代胜,梁龙飞,等. 不同添加剂对构树青贮饲料发酵品质和蛋白质降解的影响[J]. 草业学报,2021,30(10):169-179 DANNER H,HOLZER M,MAYRHUBER E,et al. Acetic acid increases stability of silage under aerobic conditions[J]. Applied and Environmental Microbiology,2003,69(1):562-567 KUNG L,SHAVER R D,GRANT R J,et al. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages[J]. Journal of Dairy Science,2018,101(5):4020-4033 DING Z T,XU D M,BAI J,et al. Characterization and identification of ferulic acid esterase-producing Lactobacillus species isolated from Elymus nutans silage and their application in ensiled alfalfa[J]. Journal of Applied Microbiology,2019,127(4):985-995 MATHEW S,ABRAHAM T E. Ferulic acid: An antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications[J]. Critical Reviews in Biotechnology,2004,24(2-3):59-83 ZHANG X,ZHANG H X,WANG D C,et al. From waste to value: Multi-omics reveal pomegranate peel addition improves corn silage antioxidant activity and reduces methane and nitrogen losses[J]. Bioresource Technology,2025,429:132544 HUANG S,KE W C,LU Q,et al. Effects of total flavonoids from Taraxacum mongolicum Hand.-Mazz. on fermentation quality, antioxidant status and microbial community of Caragana korshinskii Kom. silage[J]. Fermentation,2023,9(11):949 WANG Y,WANG W,WANG R,et al. Dietary supplementation of ferulic acid improves performance and alleviates oxidative stress of lambs in a cold environment[J]. Canadian Journal of Animal Science,2019,99(4):705-712 KHAN K A,SALEEM M H,AFZAL S,et al. Ferulic acid: therapeutic potential due to its antioxidant properties, role in plant growth, and stress tolerance[J]. Plant Growth Regulation,2024,104(3):1329-1353 JOMOVA K,ALOMAR S Y,ALWASEL S H,et al. Several lines of antioxidant defense against oxidative stress: antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants[J]. Archives of Toxicology,2024,98(5):1323-1367 ZHENG M L,LIU Y T,ZHANG G F,et al. The antioxidant properties, metabolism, application and mechanism of ferulic acid in medicine, food, cosmetics, livestock and poultry[J]. Antioxidants,2024,13(7):853 CHEN G W,NONG Z Y,CHEN N,et al. Effects of different proportions of Piper sarmentosum on the fermentation quality and in vitro fermentation characteristics of king grass silage[J]. Acta Agrestia Sinica,2025,33(5):1677-1685陈桂武,农智颜,陈娜,等. 不同比例假蒟对王草青贮发酵品质及体外发酵特性的影响[J]. 草地学报,2025,33(5):1677-1685 LÜ J Y,WANG Y X,HOU G Q,et al. Effect of co-inoculation of feruloyl esterase and Lactiplantibacillus plantarum on fermentation quality of hybrid Broussonetia papyrifera[J]. Feed Research,2025,48(5):121-125吕家英,王义翔,侯国庆,等. 阿魏酸酯酶与植物乳杆菌联合接种对杂交构树发酵品质的影响[J]. 饲料研究,2025,48(5):121-125 SHENG Z Y,WANG D F,LÜ R L,et al. Effects of Piper sarmentosum extract on in vitro ruminal fermentation parameters and dry matter digestibility in hainan black goats[J]. Chinese Journal of Animal Science,2024,60(5):233-238盛子阳,王定发,吕仁龙,等. 假蒟提取物对海南黑山羊体外瘤胃发酵参数和干物质消化率的影响[J]. 中国畜牧杂志,2024,60(5):233-238 LIU N B,WANG Y M,DIAO X G,et al. Effects of gallic acid and grape seed proanthocyanidins on fermentation quality, nutrient composition and in vitro ruminal fermentation of stylo silage[J]. Feed Industry,2025,46(15):119-125刘南冰,王奕敏,刁小高,等. 没食子酸、葡萄籽原花青素对柱花草青贮发酵品质、营养成分和体外瘤胃发酵的影响[J]. 饲料工业,2025,46(15):119-125 YU P,MCKINNON J J,CHRISTENSEN D A. Improving the nutritional value of oat hulls for ruminant animals with pretreatment of a multienzyme cocktail: In vitro studies[J]. Journal of Animal Science,2005,83(5):1133-1141 LI F H,USMAN S,HUANG W K,et al. Effects of inoculating feruloyl esterase-producing Lactiplantibacillus plantarum A1 on ensiling characteristics, in vitro ruminal fermentation and microbiota of alfalfa silage[J]. Journal of Animal Science and Biotechnology,2023,14(1):43)
Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation, Antioxidant and Digestibility of Alfalfa Baled Silage
ZHANG Yan-wei1, MAO Yu-qing1, WANG Peng1, TAO You-yan1, GUO Xu-sheng2, LI Fu-hou1
Affiliations
1 College of Animal Sciences, Jilin University, Changchun, Jilin Province 130062, China; 2 College of Life Sciences, Lanzhou University, Lanzhou, Gansu Province 730000, China
This study investigated the effects of inoculation with feruloyl esterase-producing lactic acid bacteria Lactobacillus reuteri A4-2 on the fermentation quality, antioxidant status, and in vitro digestibility of baled alfalfa silage. Chopped fresh alfalfa (Medicago sativa L.) at the early to full-blossom flowering stage was randomly divided into three groups: CON (without inoculant), YM13 (inoculated with non-feruloyl esterase-producing L. reuteri YM13), and A4-2 (inoculated with L. reuteri A4-2). Samples were collected after 20, 40, and 60 d of ensiling to determine fermentation characteristics, free ferulic acid content, antioxidant enzyme activities, chemical composition, and in vitro dry matter digestibility. Compared with the CON and YM13, inoculation with L. reuteri A4-2 significantly reduced pH but increased lactic and acetic acid contents (P<0.05). Both inoculating L. reuteri YM13 and L. reuteri A4-2 in silage showed a higher dry matter content and lower ammonia nitrogen/total nitrogen than CON (P<0.05). Inoculation with L. reuteri A4-2 in alfalfa silage markedly enhanced the levels of free ferulic acid, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) activities, and total antioxidant capacity (T-AOC) (P<0.05) relative to CON and YM13 during ensiling. In addition, inoculation with L. reuteri A4-2 effectively decreased neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents, thereby improving in vitro DM digestibility compared with the other treatments (P<0.05). Overall, inoculation with L. reuteri A4-2 not only improved the fermentation quality of silage, but also enhanced the antioxidant capacity, reduced its fiber content, and improved its in vitro DM digestibility compared with the CON and YM13 groups, demonstrating significant application advantages.
ZHANG Yan-wei, MAO Yu-qing, WANG Peng, TAO You-yan, GUO Xu-sheng, LI Fu-hou.
Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation, Antioxidant and Digestibility of Alfalfa Baled Silage[J].
Acta Agrestia Sinica,
2026
, 34
(8)
: 3131
-3140
.
DOI: 10.11733/j.issn.1007-0435.2026.08.035
基金
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国家自然科学基金项目 (32301493)
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SORDILLO L M,AITKEN S L. Impact of oxidative stress on the health and immune function of dairy cattle[J]. Veterinary Immunology and Immunopathology,2009,128(1-3):104-109 CASTILLO C,PEREIRA V,ABUELO Á,et al. Effect of supplementation with antioxidants on the quality of bovine milk and meat production[J]. The Scientific World Journal,2013,2013:616098 ABUELO A,HERNÁNDEZ J,BENEDITO J L,et al. The importance of the oxidative status of dairy cattle in the periparturient period: revisiting antioxidant supplementation[J]. Journal of Animal Physiology and Animal Nutrition,2015,99(6):1003-1016 LOURENÇO S C,MOLDÃO-MARTINS M,ALVES V D. Antioxidants of natural plant origins: From sources to food industry applications[J]. Molecules,2019,24(22):4132 WANG T,LI J,LU Q,et al. Effects of total flavonoids from Taraxacum mongolicum and Bacillus subtilis on fermentation quality, antioxidant activity, and aerobic stability of Avena sativa silage[J]. Acta Prataculturae Sinica,2025,35(6):1-14王涛,李静,卢强,等. 蒲公英黄酮和枯草芽孢杆菌对燕麦青贮品质、抗氧化活性及微生物群落结构的影响[J]. 草业学报,2025,35(6):1-14 HUO W J,ZHANG Y J,ZHANG L Y,et al. Effect of lactobacilli inoculation on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage[J]. Frontiers in Microbiology,2022,13:1070175 HORVAT D,VILJEVAC VULETIĆ M,ANDRIĆ L,et al. Characterization of forage quality, phenolic profiles, and antioxidant activity in alfalfa (Medicago sativa L.)[J]. Plants,2022,11(20):2735 BHANDARI K B,RUSCH H L,HEUSCHELE D J. Alfalfa stem cell wall digestibility: Current knowledge and future research directions[J]. Agronomy,2023,13(12):2875 KIM D,LEE K D,CHOI K C. Role of LAB in silage fermentation: Effect on nutritional quality and organic acid production-An overview[J]. Aims Agriculture and Food,2021,6(1):216-234 ZHANG Y X,USMAN S,LI Q,et al. Effects of antioxidant-rich Lactiplantibacillus plantarum inoculated alfalfa silage on rumen fermentation, antioxidant and immunity status, and mammary gland gene expression in dairy goats[J]. Journal of Animal Science and Biotechnology,2024,15(1):9 LI F H,ZHANG B B,ZHANG Y X,et al. Probiotic effect of ferulic acid esterase-producing Lactobacillus plantarum inoculated alfalfa silage on digestion, antioxidant, and immunity status of lactating dairy goats[J]. Animal Nutrition,2022,11:38-47 KHARAZIAN Z A,JIA M Y,SU R N,et al. Fermentation quality, chemical composition, and antioxidant capacity of alfalfa and corn silages inoculated with exopolysaccharide-producing lactic acid bacteria[J]. Animal Feed Science and Technology,2025,329:116490 LI F H,DING Z T,CHEN X Z,et al. The effects of Lactobacillus plantarum with feruloyl esterase-producing ability or high antioxidant activity on the fermentation, chemical composition, and antioxidant status of alfalfa silage[J]. Animal Feed Science and Technology,2021,273:114835 DONAGHY J,KELLY P F,MCKAY A M. Detection of ferulic acid esterase production by Bacillus spp. and lactobacilli[J]. Applied Microbiology and Biotechnology,1998,50(2):257-260 LI F H,DING Z T,KE W C,et al. Ferulic acid esterase-producing lactic acid bacteria and cellulase pretreatments of corn stalk silage at two different temperatures: Ensiling characteristics, carbohydrates composition and enzymatic saccharification[J]. Bioresource Technology,2019,282:211-221 KE W C,DING W R,XU D M,et al. Effects of addition of malic or citric acids on fermentation quality and chemical characteristics of alfalfa silage[J]. Journal of Dairy Science,2017,100(11):8958-8966 VAN SOEST P J,ROBERTSON J B,LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science,1991,74(10):3583-3597 ARTHUR THOMAS T. An automated procedure for the determination of soluble carbohydrates in herbage[J]. Journal of the Science of Food and Agriculture,1977,28(7):639-642 THEODOROU M K,WILLIAMS B A,DHANOA M S,et al. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds[J]. Animal Feed Science and Technology,1994,48(3-4):185-197 WU C R,DAI S,LIANG L F,et al.Effects of different additives on fermentation quality and protein degradation of Broussonetia papyrifera silage[J]. Acta Prataculturae Sinica,2021,30(10):169-179吴长荣,代胜,梁龙飞,等. 不同添加剂对构树青贮饲料发酵品质和蛋白质降解的影响[J]. 草业学报,2021,30(10):169-179 DANNER H,HOLZER M,MAYRHUBER E,et al. Acetic acid increases stability of silage under aerobic conditions[J]. Applied and Environmental Microbiology,2003,69(1):562-567 KUNG L,SHAVER R D,GRANT R J,et al. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages[J]. Journal of Dairy Science,2018,101(5):4020-4033 DING Z T,XU D M,BAI J,et al. Characterization and identification of ferulic acid esterase-producing Lactobacillus species isolated from Elymus nutans silage and their application in ensiled alfalfa[J]. Journal of Applied Microbiology,2019,127(4):985-995 MATHEW S,ABRAHAM T E. Ferulic acid: An antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications[J]. Critical Reviews in Biotechnology,2004,24(2-3):59-83 ZHANG X,ZHANG H X,WANG D C,et al. From waste to value: Multi-omics reveal pomegranate peel addition improves corn silage antioxidant activity and reduces methane and nitrogen losses[J]. Bioresource Technology,2025,429:132544 HUANG S,KE W C,LU Q,et al. Effects of total flavonoids from Taraxacum mongolicum Hand.-Mazz. on fermentation quality, antioxidant status and microbial community of Caragana korshinskii Kom. silage[J]. Fermentation,2023,9(11):949 WANG Y,WANG W,WANG R,et al. Dietary supplementation of ferulic acid improves performance and alleviates oxidative stress of lambs in a cold environment[J]. Canadian Journal of Animal Science,2019,99(4):705-712 KHAN K A,SALEEM M H,AFZAL S,et al. Ferulic acid: therapeutic potential due to its antioxidant properties, role in plant growth, and stress tolerance[J]. Plant Growth Regulation,2024,104(3):1329-1353 JOMOVA K,ALOMAR S Y,ALWASEL S H,et al. Several lines of antioxidant defense against oxidative stress: antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants[J]. Archives of Toxicology,2024,98(5):1323-1367 ZHENG M L,LIU Y T,ZHANG G F,et al. The antioxidant properties, metabolism, application and mechanism of ferulic acid in medicine, food, cosmetics, livestock and poultry[J]. Antioxidants,2024,13(7):853 CHEN G W,NONG Z Y,CHEN N,et al. Effects of different proportions of Piper sarmentosum on the fermentation quality and in vitro fermentation characteristics of king grass silage[J]. Acta Agrestia Sinica,2025,33(5):1677-1685陈桂武,农智颜,陈娜,等. 不同比例假蒟对王草青贮发酵品质及体外发酵特性的影响[J]. 草地学报,2025,33(5):1677-1685 LÜ J Y,WANG Y X,HOU G Q,et al. Effect of co-inoculation of feruloyl esterase and Lactiplantibacillus plantarum on fermentation quality of hybrid Broussonetia papyrifera[J]. Feed Research,2025,48(5):121-125吕家英,王义翔,侯国庆,等. 阿魏酸酯酶与植物乳杆菌联合接种对杂交构树发酵品质的影响[J]. 饲料研究,2025,48(5):121-125 SHENG Z Y,WANG D F,LÜ R L,et al. Effects of Piper sarmentosum extract on in vitro ruminal fermentation parameters and dry matter digestibility in hainan black goats[J]. Chinese Journal of Animal Science,2024,60(5):233-238盛子阳,王定发,吕仁龙,等. 假蒟提取物对海南黑山羊体外瘤胃发酵参数和干物质消化率的影响[J]. 中国畜牧杂志,2024,60(5):233-238 LIU N B,WANG Y M,DIAO X G,et al. Effects of gallic acid and grape seed proanthocyanidins on fermentation quality, nutrient composition and in vitro ruminal fermentation of stylo silage[J]. Feed Industry,2025,46(15):119-125刘南冰,王奕敏,刁小高,等. 没食子酸、葡萄籽原花青素对柱花草青贮发酵品质、营养成分和体外瘤胃发酵的影响[J]. 饲料工业,2025,46(15):119-125 YU P,MCKINNON J J,CHRISTENSEN D A. Improving the nutritional value of oat hulls for ruminant animals with pretreatment of a multienzyme cocktail: In vitro studies[J]. Journal of Animal Science,2005,83(5):1133-1141 LI F H,USMAN S,HUANG W K,et al. Effects of inoculating feruloyl esterase-producing Lactiplantibacillus plantarum A1 on ensiling characteristics, in vitro ruminal fermentation and microbiota of alfalfa silage[J]. Journal of Animal Science and Biotechnology,2023,14(1):43