Article(id=1309574974193623310, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.08.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1773676800000, receivedDateStr=2026-03-17, revisedDate=1775059200000, revisedDateStr=2026-04-02, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156604574, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156604574, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156604574, creator=13701087609, updateTime=1790156604574, updator=13701087609, issue=Issue{id=1309574965775659485, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='8', pageStart='2753', pageEnd='3140', issueExtLink='null', onlineDate='null', pubDate='1786723200000', pubDateStr='2026-08-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156602567, creator='13701087609', updateTime=1790156935415, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309576361992352087, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309576361992352088, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309574965775659485, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2814, endPage=2825, ext={EN=ArticleExt(id=1309574974856323345, articleId=1309574974193623310, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=GWAS-Based Identification of Markers and Candidate Genes for Seed Setting Rate and Seed Yield in Leymus chinensis, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Leymus chinensis is the main dominant species in the eastern Eurasian steppe, with significant ecological and production values. However, its low seed yield severely restricts its practical application. To identify the associated loci for the seed yield traits of L. chinensis and to discover key molecular markers, this study used 300 diverse accessions of L. chinensis as materials, measured five yield traits including spikelet number per spike, floret number per spikelet, total number of florets, seed number per spikelet and seed setting rate, and conducted a genome-wide association analysis (GWAS) using a mixed linear model. The results showed that seed number per spikelet exhibited the highest coefficient of variation (CV=0.52), indicating substantial potential for genetic improvement. The seed setting rate was regulated by an independent genetic mechanism and was the key limiting factor for seed yield. GWAS analysis combined with gene functional annotation identified 120 key single nucleotide polymorphisms (SNPs) and identified EZ1 and multiple MADS-box and WRKY transcription factors as key candidate genes regulating seed yield. Based on 14 SNPs significantly associated with seed setting rate, 8 elite alleles were obtained. Notably, the CT genotype at the Lc5Ns_97563471 locus exhibited a high mean seed setting rate of 66.6%. This study revealed the genetic characteristics of L. chinensis seed yield being regulated by multiple genes, and the identified candidate genes and elite SNP loci can serve as direct targets for molecular marker-assisted breeding., authors=WEI Jia1, LIU Zhen-zhen1, XU Xin-xin1, MU Lin-lin2, DONG Hong-bin3, GAO Qiu3, HONG Jun3, WANG Jian-li2, MA Xi-qing1, authorsList=WEI Jia, LIU Zhen-zhen, XU Xin-xin, MU Lin-lin, DONG Hong-bin, GAO Qiu, HONG Jun, WANG Jian-li, MA Xi-qing, authorCompany=1 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2 Institute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China;
3 National Animal Husbandry Service, Beijing 100125, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574974768242960, articleId=1309574974193623310, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=基于GWAS挖掘羊草结实率及种子产量相关标记与候选基因, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=羊草(Leymus chinensis)是欧亚草原东部的主要建群种,生态与生产价值显著,但种子产量低严重制约其产业化应用。为定位羊草种子产量性状的关联位点,挖掘关键分子标记,本研究以300份羊草种质为材料,测定小穗数、每穗小花数、总小花数、每穗种子数及结实率5个产量性状,采用混合线性模型进行全基因组关联分析(Genome wide association study, GWAS),挖掘和羊草结实率性状相关的分子标记和候选基因。结果表明,每穗种子数变异幅度最大(CV=0.52),遗传改良潜力高;结实率是种子产量的关键限制因子,受独立的遗传机制调控。GWAS分析结合基因功能注释共筛选出120个关键单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,并鉴定到调控种子产量的关键候选基因EZ1及多个MADS-box,WRKY类等转录因子。基于14个与结实率显著关联的SNP位点,共获得8个优异等位变异,其中,位点Lc5Ns_97563471的CT基因型结实率均值高达66.6%。本研究揭示了羊草种子产量受多基因调控的遗传特性,获得的候选基因及优异SNP位点可直接用于分子标记辅助育种。, authors=魏佳1, 刘真真1, 徐欣欣1, 牟林林2, 董弘斌3, 高秋3, 洪军3, 王建丽2, 马西青1, authorsList=魏佳, 刘真真, 徐欣欣, 牟林林, 董弘斌, 高秋, 洪军, 王建丽, 马西青, authorCompany=1 中国农业大学草业科学与技术学院, 北京 100193;
2 黑龙江省农业科学院草业研究所, 黑龙江 哈尔滨 150086;
3 全国畜牧总站, 北京 100125, correspAuthors=null, authorNote=魏佳(2002-),女,汉族,河南新蔡人,博士研究生,主要从事草类育种与种子科学研究,E-mail:weijia_ke@126.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=+KQ9j8RKJCnI+jf5sAynFQ==, pdfFileSize=2552313, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, keywords=[Keyword(id=1309574975053455634, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574974193623310, language=CN, orderNo=1, keyword=羊草), Keyword(id=1309574976731177235, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574974193623310, language=CN, orderNo=2, keyword=结实率), Keyword(id=1309574976831840532, tenantId=1146029695717560320, journalId=1308397196074385509, 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WANG Q,YANG Z P. Effects of mowing and fertilization on seed yield of Leymus chinensis in Inner Mongolia steppe[J]. Chinese Journal of Grassland,2022,44(12):100-106王迁,杨兆平. 刈割和施肥对内蒙古典型草原羊草种子产量的影响[J]. 中国草地学报,2022,44(12):100-106
LIU Z D,CHAI F J,GAO H J,et al. Effects of trace elements on seed setting rate of Leymus chinensis[J]. Heilongjiang Agricultural Sciences,2019(8):131-134刘泽东,柴凤久,高海娟,等. 施用微量元素对羊草结实率的影响[J]. 黑龙江农业科学,2019(8):131-134
CAO K,ZHOU Z K,WANG Q,et al. Genome-wide association study of 12 agronomic traits in peach[J]. Nature Communications,2016,7:13246
WANG K X. Association analysis of maize ear-related traits and genomic selection breeding[D]. Urumqi:Xinjiang Agricultural University,2025:19-42汪凯翔. 玉米穗部相关性状关联分析与全基因组选择育种[D]. 乌鲁木齐:新疆农业大学,2025:19-42
LIU M C,ZHANG Y D,SHAW R K,et al. Genome-wide association study and prediction of tassel weight of tropical maize germplasm in multi-parent population[J]. International Journal of Molecular Sciences,2024,25(3):1756
BAI X H. Genome-wide association analysis of the grain numbers in the basal spikelets in wheat and mining of key candidate genes[D]. Taigu:Shanxi Agricultural University,2023:27-34白雄辉. 小麦穗基部结实粒数全基因组关联分析及关键候选基因的挖掘[D]. 太谷:山西农业大学,2023:27-34
LI T,TANG S J,LI W,et al. Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe[J]. Proceedings of the National Academy of Sciences of the United States of America,2023,120(44):1-10
YU H. Studies on the effects of optimal nitrogen application and irrigation time on seed yield and yield components of Leymus chinensis[D]. Changchun:Northeast Normal University,2010:1-2于辉. 羊草种子产量构成因子与产量最适调控时间的研究[D]. 长春:东北师范大学,2010:1-2
WANG J. Study on the low seed setting percentage of Leymus chinensis based on photosynthetic product distribution[D]. Changchun:Northeast Normal University,2020:24-25王姣. 基于光合产物转运分配的羊草低结实率研究[D]. 长春:东北师范大学,2020:24-25
CHEN Z,NIU J P,CAO X L,et al. Seed yield can be explained by altered yield components in field-grown western wheatgrass (Pascopyrum smithii Rydb.)[J]. Scientific Reports,2019,9:17976
KIM J Y,SONG J T,SEO H S. The enhancer of zeste gene OsiEZ1is involved in ligule and seed development in rice[J]. Canadian Journal of Plant Science,2017:1068-1074
MIZZOTTI C,MENDES M A,CAPORALI E,et al. The MADS box genes SEEDSTICK and ARABIDOPSIS Bsister play a maternal role in fertilization and seed development[J]. The Plant Journal,2012,70(3):409-420
YU Y F,CHU X,MA X J,et al. Genome-wide analysis of MADS-box gene family reveals CjSTK as a key regulator of seed abortion in Camellia japonica[J]. International Journal of Molecular Sciences,2024,25(11):5770
TANG J Q,TIAN X J,MEI E Y,et al. WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels[J]. The Plant Cell,2022,34(11):4495-4515
TIAN C Y,BIAN X,LANG R B,et al. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane[J]. Acta Agronomica Sinica,2024,50(2):310-324田春艳,边芯,郎荣斌,等. 甘蔗3个育种性状与SSR标记的关联分析及优异等位变异发掘[J]. 作物学报,2024,50(2):310-324)
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基于GWAS挖掘羊草结实率及种子产量相关标记与候选基因
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草地学报 | 研究论文 2026,34(8): 2814-2825
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草地学报 |研究论文 2026 , 34 (8) : 2814 -2825
基于GWAS挖掘羊草结实率及种子产量相关标记与候选基因
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魏佳1, 刘真真1, 徐欣欣1, 牟林林2, 董弘斌3, 高秋3, 洪军3, 王建丽2, 马西青1
作者信息
    1 中国农业大学草业科学与技术学院, 北京 100193;
    2 黑龙江省农业科学院草业研究所, 黑龙江 哈尔滨 150086;
    3 全国畜牧总站, 北京 100125
作者简介:
魏佳(2002-),女,汉族,河南新蔡人,博士研究生,主要从事草类育种与种子科学研究,E-mail:weijia_ke@126.com
GWAS-Based Identification of Markers and Candidate Genes for Seed Setting Rate and Seed Yield in Leymus chinensis
  • WEI Jia1, LIU Zhen-zhen1, XU Xin-xin1, MU Lin-lin2, DONG Hong-bin3, GAO Qiu3, HONG Jun3, WANG Jian-li2, MA Xi-qing1
  • Affiliations
      1 College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
      2 Institute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China;
      3 National Animal Husbandry Service, Beijing 100125, China
    doi: 10.11733/j.issn.1007-0435.2026.08.006
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    羊草(Leymus chinensis)是欧亚草原东部的主要建群种,生态与生产价值显著,但种子产量低严重制约其产业化应用。为定位羊草种子产量性状的关联位点,挖掘关键分子标记,本研究以300份羊草种质为材料,测定小穗数、每穗小花数、总小花数、每穗种子数及结实率5个产量性状,采用混合线性模型进行全基因组关联分析(Genome wide association study, GWAS),挖掘和羊草结实率性状相关的分子标记和候选基因。结果表明,每穗种子数变异幅度最大(CV=0.52),遗传改良潜力高;结实率是种子产量的关键限制因子,受独立的遗传机制调控。GWAS分析结合基因功能注释共筛选出120个关键单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,并鉴定到调控种子产量的关键候选基因EZ1及多个MADS-box,WRKY类等转录因子。基于14个与结实率显著关联的SNP位点,共获得8个优异等位变异,其中,位点Lc5Ns_97563471的CT基因型结实率均值高达66.6%。本研究揭示了羊草种子产量受多基因调控的遗传特性,获得的候选基因及优异SNP位点可直接用于分子标记辅助育种。
    羊草  /  结实率  /  种子产量  /  全基因组关联分析  /  优异等位变异
    Leymus chinensis is the main dominant species in the eastern Eurasian steppe, with significant ecological and production values. However, its low seed yield severely restricts its practical application. To identify the associated loci for the seed yield traits of L. chinensis and to discover key molecular markers, this study used 300 diverse accessions of L. chinensis as materials, measured five yield traits including spikelet number per spike, floret number per spikelet, total number of florets, seed number per spikelet and seed setting rate, and conducted a genome-wide association analysis (GWAS) using a mixed linear model. The results showed that seed number per spikelet exhibited the highest coefficient of variation (CV=0.52), indicating substantial potential for genetic improvement. The seed setting rate was regulated by an independent genetic mechanism and was the key limiting factor for seed yield. GWAS analysis combined with gene functional annotation identified 120 key single nucleotide polymorphisms (SNPs) and identified EZ1 and multiple MADS-box and WRKY transcription factors as key candidate genes regulating seed yield. Based on 14 SNPs significantly associated with seed setting rate, 8 elite alleles were obtained. Notably, the CT genotype at the Lc5Ns_97563471 locus exhibited a high mean seed setting rate of 66.6%. This study revealed the genetic characteristics of L. chinensis seed yield being regulated by multiple genes, and the identified candidate genes and elite SNP loci can serve as direct targets for molecular marker-assisted breeding.
    Leymus chinensis  /  Seed setting rate  /  Seed yield  /  Genome-wide association study  /  Superior allelic variation
    魏佳, 刘真真, 徐欣欣, 牟林林, 董弘斌, 高秋, 洪军, 王建丽, 马西青. 基于GWAS挖掘羊草结实率及种子产量相关标记与候选基因. 草地学报, 2026 , 34 (8) : 2814 -2825 . DOI: 10.11733/j.issn.1007-0435.2026.08.006
    WEI Jia, LIU Zhen-zhen, XU Xin-xin, MU Lin-lin, DONG Hong-bin, GAO Qiu, HONG Jun, WANG Jian-li, MA Xi-qing. GWAS-Based Identification of Markers and Candidate Genes for Seed Setting Rate and Seed Yield in Leymus chinensis[J]. Acta Agrestia Sinica, 2026 , 34 (8) : 2814 -2825 . DOI: 10.11733/j.issn.1007-0435.2026.08.006

    参考文献 引证文献
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    CAI Y,LI Z Y,YU X H,et al. Effects of appropriate nitrogen fertilizer on seed productivity and forage quality of Leymus chinensis grassland in western Jilin Province[J]. Agriculture and Technology,2022,42(1):16-20才源,李子勇,于新海,等. 适量氮肥对吉林西部人工建植羊草草原的种子产能与牧草品质影响的研究[J]. 农业与技术,2022,42(1):16-20
    WANG Q,YANG Z P. Effects of mowing and fertilization on seed yield of Leymus chinensis in Inner Mongolia steppe[J]. Chinese Journal of Grassland,2022,44(12):100-106王迁,杨兆平. 刈割和施肥对内蒙古典型草原羊草种子产量的影响[J]. 中国草地学报,2022,44(12):100-106
    LIU Z D,CHAI F J,GAO H J,et al. Effects of trace elements on seed setting rate of Leymus chinensis[J]. Heilongjiang Agricultural Sciences,2019(8):131-134刘泽东,柴凤久,高海娟,等. 施用微量元素对羊草结实率的影响[J]. 黑龙江农业科学,2019(8):131-134
    CAO K,ZHOU Z K,WANG Q,et al. Genome-wide association study of 12 agronomic traits in peach[J]. Nature Communications,2016,7:13246
    WANG K X. Association analysis of maize ear-related traits and genomic selection breeding[D]. Urumqi:Xinjiang Agricultural University,2025:19-42汪凯翔. 玉米穗部相关性状关联分析与全基因组选择育种[D]. 乌鲁木齐:新疆农业大学,2025:19-42
    LIU M C,ZHANG Y D,SHAW R K,et al. Genome-wide association study and prediction of tassel weight of tropical maize germplasm in multi-parent population[J]. International Journal of Molecular Sciences,2024,25(3):1756
    BAI X H. Genome-wide association analysis of the grain numbers in the basal spikelets in wheat and mining of key candidate genes[D]. Taigu:Shanxi Agricultural University,2023:27-34白雄辉. 小麦穗基部结实粒数全基因组关联分析及关键候选基因的挖掘[D]. 太谷:山西农业大学,2023:27-34
    LI T,TANG S J,LI W,et al. Genome evolution and initial breeding of the Triticeae grass Leymus chinensis dominating the Eurasian Steppe[J]. Proceedings of the National Academy of Sciences of the United States of America,2023,120(44):1-10
    YU H. Studies on the effects of optimal nitrogen application and irrigation time on seed yield and yield components of Leymus chinensis[D]. Changchun:Northeast Normal University,2010:1-2于辉. 羊草种子产量构成因子与产量最适调控时间的研究[D]. 长春:东北师范大学,2010:1-2
    WANG J. Study on the low seed setting percentage of Leymus chinensis based on photosynthetic product distribution[D]. Changchun:Northeast Normal University,2020:24-25王姣. 基于光合产物转运分配的羊草低结实率研究[D]. 长春:东北师范大学,2020:24-25
    CHEN Z,NIU J P,CAO X L,et al. Seed yield can be explained by altered yield components in field-grown western wheatgrass (Pascopyrum smithii Rydb.)[J]. Scientific Reports,2019,9:17976
    KIM J Y,SONG J T,SEO H S. The enhancer of zeste gene OsiEZ1is involved in ligule and seed development in rice[J]. Canadian Journal of Plant Science,2017:1068-1074
    MIZZOTTI C,MENDES M A,CAPORALI E,et al. The MADS box genes SEEDSTICK and ARABIDOPSIS Bsister play a maternal role in fertilization and seed development[J]. The Plant Journal,2012,70(3):409-420
    YU Y F,CHU X,MA X J,et al. Genome-wide analysis of MADS-box gene family reveals CjSTK as a key regulator of seed abortion in Camellia japonica[J]. International Journal of Molecular Sciences,2024,25(11):5770
    TANG J Q,TIAN X J,MEI E Y,et al. WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels[J]. The Plant Cell,2022,34(11):4495-4515
    TIAN C Y,BIAN X,LANG R B,et al. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane[J]. Acta Agronomica Sinica,2024,50(2):310-324田春艳,边芯,郎荣斌,等. 甘蔗3个育种性状与SSR标记的关联分析及优异等位变异发掘[J]. 作物学报,2024,50(2):310-324
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    doi: 10.11733/j.issn.1007-0435.2026.08.006
    • 接收时间:2026-03-17
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    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
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