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PEG osmotic priming was combined with EMS chemical mutagenesis to screen for optimum compound mutagenesis conditions for forage oat. Based on the germination characteristics and seedling traits of the M1 generation in the two oat cultivars, the pretreatment conditions of 20% PEG for 3 h and 10% PEG for 6 h were found to promote seedling growth. The optimal EMS treatment conditions were 1% EMS for 16 h for ‘Zhongtian No.4’ and 0.75% EMS for 16 h for ‘Qinghai 444’. Through a comprehensive analysis of field agronomic traits at the milk stage in the M2 generation after combined PEG and EMS treatments, it was found that 30% PEG for 3 h combined with the optimal EMS treatment produced the best results for developing germplasm with high-yield potential in both cultivars. Under these conditions, the mutagenesis efficiencies of the M2 population were 2.40% for ‘Zhongtian No. 4’ and 1.26% for ‘Qinghai 444’, respectively, and a total of 22 germplasm lines with high-yield potential were obtained. These results provide a rich material basis for subsequent oat breeding., authors=ZHONG Zhi-hua1 , LU Mao-jin1 , YANG Guo-dong1 , LIU Chao1 , WANG Rong1 , YAN Ming-ke1 , GUO Huan1 , MA Xiang2 , WANG Ya-fang1 , authorsList=ZHONG Zhi-hua, LU Mao-jin, YANG Guo-dong, LIU Chao, WANG Rong, YAN Ming-ke, GUO Huan, MA Xiang, WANG Ya-fang, authorCompany=1 College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China; 2 Academy of Animal and Veterinary Science, Qinghai University, Xining, Qinghai Province 810016, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574972473958656, articleId=1309574970783654143, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=PEG前处理对饲用燕麦种子EMS诱变效应的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为优化饲用燕麦(Avena sativa L.)诱变技术,提高饲用燕麦的诱变效率,本研究以‘青海444’和‘中天4号’种子为材料,采用PEG渗透调节前处理协同EMS化学诱变处理,筛选高效的饲用燕麦复合诱变条件。综合两个燕麦品种M₁代种子发芽特征及种苗特性,发现20% PEG 3 h和10% PEG 6 h 前处理条件能够促进幼苗生长;两个燕麦品种的最适EMS处理条件分别为1% EMS 16 h(‘中天4号’)和0.75% EMS 16 h(‘青海444’)。通过对PEG和EMS复合处理后M₂代乳熟期田间农艺性状综合分析,发现两个品种在30% PEG处理3 h协同最适EMS处理条件,在创制高产潜力种质方面效果最佳,其M₂代诱变效率分别为2.40%(‘中天4号’)和1.26%(‘青海444’),共获得具有高产潜力的种质材料22份,为后续燕麦育种提供了变异丰富的材料基础。, authors=钟志花1 , 卢茂金1 , 杨国栋1 , 刘朝1 , 王蓉1 , 闫明科1 , 郭欢1 , 马祥2 , 王亚芳1 , authorsList=钟志花, 卢茂金, 杨国栋, 刘朝, 王蓉, 闫明科, 郭欢, 马祥, 王亚芳, authorCompany=1 西北农林科技大学草业与草原学院, 陕西 杨凌 712100; 2 青海大学畜牧兽医科学院, 青海 西宁 810016, correspAuthors=null, authorNote=钟志花(2001-),女,汉族,青海海东人,硕士研究生,主要从事牧草种质资源与遗传育种研究,E-mail:zhihua@nwafu.edu.cn, 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草地学报
|研究论文
2026
, 34
(8) :
2857
-2865
PEG前处理对饲用燕麦种子EMS诱变效应的影响
全屏
钟志花1 , 卢茂金1 , 杨国栋1 , 刘朝1 , 王蓉1 , 闫明科1 , 郭欢1 , 马祥2 , 王亚芳1
作者信息
1 西北农林科技大学草业与草原学院, 陕西 杨凌 712100; 2 青海大学畜牧兽医科学院, 青海 西宁 810016
作者简介:
钟志花(2001-),女,汉族,青海海东人,硕士研究生,主要从事牧草种质资源与遗传育种研究,E-mail:zhihua@nwafu.edu.cn
Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat(Avena sativa L.)Seeds
ZHONG Zhi-hua1 , LU Mao-jin1 , YANG Guo-dong1 , LIU Chao1 , WANG Rong1 , YAN Ming-ke1 , GUO Huan1 , MA Xiang2 , WANG Ya-fang1
Affiliations
1 College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China; 2 Academy of Animal and Veterinary Science, Qinghai University, Xining, Qinghai Province 810016, China
doi: 10.11733/j.issn.1007-0435.2026.08.010
文章导航
为优化饲用燕麦(Avena sativa L.)诱变技术,提高饲用燕麦的诱变效率,本研究以‘青海444’和‘中天4号’种子为材料,采用PEG渗透调节前处理协同EMS化学诱变处理,筛选高效的饲用燕麦复合诱变条件。综合两个燕麦品种M₁代种子发芽特征及种苗特性,发现20% PEG 3 h和10% PEG 6 h 前处理条件能够促进幼苗生长;两个燕麦品种的最适EMS处理条件分别为1% EMS 16 h(‘中天4号’)和0.75% EMS 16 h(‘青海444’)。通过对PEG和EMS复合处理后M₂代乳熟期田间农艺性状综合分析,发现两个品种在30% PEG处理3 h协同最适EMS处理条件,在创制高产潜力种质方面效果最佳,其M₂代诱变效率分别为2.40%(‘中天4号’)和1.26%(‘青海444’),共获得具有高产潜力的种质材料22份,为后续燕麦育种提供了变异丰富的材料基础。
饲用燕麦
/
诱变
/
EMS
/
PEG
/
高产
/
种质
To optimize the EMS-induced mutagenesis technology of forage oat (Avena sativa L.) and improve mutagenic efficiency, seeds of Oat cultivar ‘Qinghai 444’ and ‘Zhongtian No. 4’ were used as materials in this study. PEG osmotic priming was combined with EMS chemical mutagenesis to screen for optimum compound mutagenesis conditions for forage oat. Based on the germination characteristics and seedling traits of the M1 generation in the two oat cultivars, the pretreatment conditions of 20% PEG for 3 h and 10% PEG for 6 h were found to promote seedling growth. The optimal EMS treatment conditions were 1% EMS for 16 h for ‘Zhongtian No.4’ and 0.75% EMS for 16 h for ‘Qinghai 444’. Through a comprehensive analysis of field agronomic traits at the milk stage in the M2 generation after combined PEG and EMS treatments, it was found that 30% PEG for 3 h combined with the optimal EMS treatment produced the best results for developing germplasm with high-yield potential in both cultivars. Under these conditions, the mutagenesis efficiencies of the M2 population were 2.40% for ‘Zhongtian No. 4’ and 1.26% for ‘Qinghai 444’, respectively, and a total of 22 germplasm lines with high-yield potential were obtained. These results provide a rich material basis for subsequent oat breeding.
Forage Oat
/
Mutagenesis
/
EMS
/
PEG
/
High yield
/
Germplasm
钟志花, 卢茂金, 杨国栋, 刘朝, 王蓉, 闫明科, 郭欢, 马祥, 王亚芳.
PEG前处理对饲用燕麦种子EMS诱变效应的影响.
草地学报,
2026
, 34
(8)
: 2857
-2865
.
DOI: 10.11733/j.issn.1007-0435.2026.08.010
ZHONG Zhi-hua, LU Mao-jin, YANG Guo-dong, LIU Chao, WANG Rong, YAN Ming-ke, GUO Huan, MA Xiang, WANG Ya-fang.
Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat(Avena sativa L.)Seeds[J].
Acta Agrestia Sinica ,
2026
, 34
(8)
: 2857
-2865
.
DOI: 10.11733/j.issn.1007-0435.2026.08.010
参考文献
引证文献
ZHANG B,REN C Z. Advances in oat genomic research and molecular breeding[J]. Bulletin of Botany ,2022,57(6):785-791张波,任长忠. 燕麦基因组学与分子育种研究进展[J]. 植物学报,2022,57(6):785-791 WEI J,GAO Y M,WANG H,et al. Phenotype genetic diversity analysis and screening for elite germplasm resources in 465 oat accessions[J]. Acta Agrestia Sinica ,2026,34(3):840-852卫纪,高杨淼,汪辉,等. 465份燕麦种质资源表型遗传多样性分析及优异资源筛选[J]. 草地学报,2026,34(3):840-852 ZHANG Z Z,ZHENG C Z,HE Z Q,et al. Identification and evaluation on genetic diversity of agronomic traits in 91Avena sativa germplasm resources[J]. Seed ,2025,44(7):106-112张子臻,郑成忠,何竹青,等. 91份燕麦种质资源农艺性状遗传多样性的鉴定评价[J]. 种子,2025,44(7):106-112 REN J H,SUN J J,HAO Y L,et al. Screening of feed oat varieties and its evaluation of silage quality in central Inner Mongolia[J]. Scientia Agricultura Sinica ,2025,58(19):4026-4038任家辉,孙娟娟,郝莹璐,等. 内蒙古中部地区饲用燕麦品种筛选及青贮品质评价[J]. 中国农业科学,2025,58(19):4026-4038 ZHOU Q P,YAN H B,LIANG G L,et al. Analysis of the forage and grain productivity of oat cultivars[J]. Acta Prataculturae Sinica ,2015,24(10):120-130周青平,颜红波,梁国玲,等. 不同燕麦品种饲草和籽粒生产性能分析[J]. 草业学报,2015,24(10):120-130 LIU W H,JIA Z F,LIANG G L. Present situation,existing problems and suggestions of feed oat industry in China[J]. Qinghai Science and Technology ,2020,27(3):82-85刘文辉,贾志锋,梁国玲. 我国饲用燕麦产业发展现状及存在的问题和建议[J]. 青海科技,2020,27(3):82-85 REN C Z,CUI L,YANG C,et al. Establishment and application of high efficient breeding technology system of oat in China[J]. Journal of Agricultural Science and Technology ,2016,18(1):1-6任长忠,崔林,杨才,等. 我国燕麦高效育种技术体系创建与应用[J]. 中国农业科技导报(中英文),2016,18(1):1-6 DORVLO I K,NI F,WANG H W. Harnessing ethyl methanesulfonate (EMS) mutagenesis and multi-omics for wheat rust resistance gene discovery[J]. Trends in Plant Science ,2025,30(12):1405-1406 WANG H J,SUN F J,SHI Z Y,et al. A forward genetics strategy for high-throughput gene identification via precise image-based phenotyping of an indexed EMS mutant library[J]. Advanced Science ,2025,12(47):e14793 GUO X P,ZHANG S H,JIA C L,et al. Heavy ion radiation-based mutation breeding driven by multi-technology integration: from mechanism to application[J]. Acta Microbiologica Sinica ,2026,66(4):1569-1584郭晓鹏,张书翰,贾承霖,等. 多技术融合驱动的重离子辐射诱变育种:从机制到应用[J]. 微生物学报,2026,66(4):1569-1584 SHI H C,CHEN C J,XIA W,et al. Analysis on mutagenic effects of EMS on three maize inbred lines[J]. Guihaia ,2016,36(8):906-914石海春,陈超杰,夏伟,等. EMS对三个玉米自交系的诱变效应分析[J]. 广西植物,2016,36(8):906-914 LI Y,ZHAO Y W,CHEN Y,et al. Effects of chemical mutagens on seed germination and main agronomic traits of M1 generation of Flax ‘Dingya 23'[J]. Crops ,2025(5):155-164李瑛,赵永伟,陈英,等. 化学诱变对胡麻‘定亚23号’种子萌发和M1代主要农艺性状的影响[J]. 作物杂志,2025(5):155-164 JIANG R,ZHAO Y J,YUAN L,et al. Effects of different chemical mutagens on seed germination of ramie[J]. Plant Fiber Sciences in China ,2022,44(5):278-284蒋榕,赵亿嘉,袁林,等. 不同化学诱变剂对苎麻种子萌发的影响[J]. 中国麻业科学,2022,44(5):278-284 WANG B,ZHAO L,HOU J J. Effects of EMS mutagenesis on seed germination of M0 generation,agronomic and quality traits of M3 generation in five flax varieties[J]. Acta Agriculturae Boreali-occidentalis Sinica ,2022,31(9):1085-1093王斌,赵利,侯静静. EMS诱变对不同基因型胡麻种子萌发及M3农艺性状和品质性状的影响[J]. 西北农业学报,2022,31(9):1085-1093 CHEN C X,GE F Y,DU H L,et al. A comprehensive omics resource and genetic tools for functional genomics research and genetic improvement of sorghum[J]. Molecular Plant ,2025,18(4):703-719 LIU X N,BAO Q J,PAN Y D,et al. Effect of EMS on germination and seedling stage of beer barley Ganpi No.8[J]. Seed ,2022,41(11):78-84柳小宁,包奇军,潘永东,等. EMS对甘啤8号大麦种子萌发期及苗期的影响[J]. 种子,2022,41(11):78-84 HUO P J,WU B,ZHANG Z W. Identification and analysis of EMS mutant library in oat (Avena nuda L.)[J]. Journal of Plant Genetic Resources ,2015,16(2):379-384霍朋杰,吴斌,张宗文. 裸燕麦EMS突变体库筛选与分析[J]. 植物遗传资源学报,2015,16(2):379-384 YANG L L. A method for improving the in vitro EMS mutagenesis efficiency in daylily (Hemerocallis hybrida ) via osmotic stress treatment: China, CN201810109647.6[P]. 2018-08-10杨丽莉,刘化涛,杨睿,等. 一种渗透胁迫处理提高大花萱草EMS离体诱变率的方法:CN108377909A[P]. 2018-08-10 CHEN X M. A method for creating mutants of red kidney bean (Phaseolus vulgaris ) using EMS mutagen and its application: China, CN201810117101.5[P]. 2021-08-03陈喜明,韩云丽,邵林生,等. 一种采用EMS诱变剂创制红芸豆突变体的方法及应用:CN108450325B[P]. 2021-08-03 SU W J,ZHAO G Q,JU Z L,et al. Effects of drought stress duration on physiological indexes of 6 oat germplasms at germination stage[J]. Acta Agrestia Sinica ,2022,30(3):646-654苏玮娟,赵桂琴,琚泽亮,等. 干旱胁迫时间对6份饲用燕麦种质萌发期生理指标的影响[J]. 草地学报,2022,30(3):646-654 YANG X. Researches on callus induction and differentiation of wheat mature embryos[D]. Lanzhou:Gansu Agricultural University,2015:69杨筱. 小麦成熟胚愈伤组织诱导及分化的研究[D]. 兰州:甘肃农业大学,2015:69 FANG L,SHANG Z Q. Osmotic adjustment technology and its application in seed treatment[J]. 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2026年第34卷第8期
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doi: 10.11733/j.issn.1007-0435.2026.08.010
接收时间:2026-02-23
首发时间:2026-09-23
收稿日期:2026-02-23
修回日期:2026-04-19
https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.08.010
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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