草地学报

蒺藜苜蓿响应盐和碱胁迫的转录组学分析

PDF下载

草地学报 | 研究论文 2026,34(7): 2415-2428

收起

草地学报 |研究论文 2026 , 34 (7) : 2415 -2428

蒺藜苜蓿响应盐和碱胁迫的转录组学分析

全屏

岳诗瑶^1,2, 梁燕霞², 闫依诺², 代淑杰², 李文翔², 解鑫磊², 肖素娟², 王玉祥¹, 江文波^2,3

作者信息

作者简介:

岳诗瑶（2001-），女，汉族，山东东营人，硕士研究生，主要从事牧草种质资源研究，E-mail:1606019547@qq.com

Transcriptomic Analysis of Salt and Alkali Stress-Responsive Genes in Medicago truncatula

YUE Shi-yao^1,2, LIANG Yan-xia², YAN Yi-nuo², DAI Shu-jie², LI Wen-xiang², XIE Xin-lei², XIAO Su-juan², WANG Yu-xiang¹, JIANG Wen-bo^2,3

Affiliations

doi: 10.11733/j.issn.1007-0435.2026.07.007

文章导航

摘要

收起

本研究采用高通量转录组测序技术，分析蒺藜苜蓿（Medicago truncatula）在盐胁迫（150 mmol·L⁻¹ NaCl）和碱胁迫（135 mmol·L⁻¹ NaHCO₃+15 mmol·L⁻¹ Na₂CO₃）下，0 h，6 h，12 h，24 h，48 h 五个时间点的基因表达变化。结果显示，碱胁迫诱导的基因变化更剧烈，下调基因普遍多于上调基因；差异表达基因显著富集于脱落酸信号转导、渗透调节、抗氧化防御及离子平衡等通路。盐与碱胁迫下共上调基因 60 个、共下调基因 16 个，上调基因涉及激素调控、信号转导等抗逆机制，下调基因涵盖激素信号、细胞壁重构等多个功能类别。本研究揭示了蒺藜苜蓿响应盐碱胁迫的关键基因，筛选出耐盐耐碱候选基因资源，为苜蓿分子育种提供理论基础。

关键词

蒺藜苜蓿 / 盐和碱胁迫 / 转录组分析 / 耐逆机制 / 差异表达基因

Abstract

收起

This study employed high-throughput transcriptome sequencing to analyze gene expression changes in Medicago truncatula under salt stress （150 mmol·L^-1 NaCl） and alkali stress （135 mmol·L^-1 NaHCO₃+15 mmol·L^-1 Na₂CO₃） at five time points： 0 h， 6 h， 12 h，24 h and 48 h. The results showed that alkali stress induced more drastic gene expression changes， with downregulated genes generally outnumbering upregulated ones. Differentially expressed genes were significantly enriched in pathways related to ABA signaling transduction， osmotic regulation， antioxidant defense， and ion homeostasis. A total of 60 genes were commonly upregulated and 16 genes were commonly downregulated under both salt and alkali stresses. The commonly upregulated genes were mainly involved in stress-resistance mechanisms such as hormone regulation and signal transduction， whereas the commonly downregulated genes covered multiple functional categories including hormone signaling and cell wall remodeling. This study elucidates key genes involved in the responses of M. truncatula to salt and alkali stresses and identifies candidate genes for salt and alkali tolerance， providing a theoretical basis for molecular breeding of alfalfa.

Key words

Medicago truncatula / Salt and alkali stress / Transcriptome analysis / Mechanisms of stress tolerance / Differentially expressed genes

引用本文

岳诗瑶, 梁燕霞, 闫依诺, 代淑杰, 李文翔, 解鑫磊, 肖素娟, 王玉祥, 江文波. 蒺藜苜蓿响应盐和碱胁迫的转录组学分析. 草地学报, 2026 , 34 (7) : 2415 -2428 . DOI: 10.11733/j.issn.1007-0435.2026.07.007

YUE Shi-yao, LIANG Yan-xia, YAN Yi-nuo, DAI Shu-jie, LI Wen-xiang, XIE Xin-lei, XIAO Su-juan, WANG Yu-xiang, JIANG Wen-bo. Transcriptomic Analysis of Salt and Alkali Stress-Responsive Genes in Medicago truncatula[J]. Acta Agrestia Sinica, 2026 , 34 (7) : 2415 -2428 . DOI: 10.11733/j.issn.1007-0435.2026.07.007

基金

收起

北方特色经济作物种质资源精准鉴定 (2023YFD1200203); 国家盐碱地综合利用技术创新中心资助

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

WANG Y T. The ministry of agriculture has issued the “National alfalfa industry development plan (2016-2020)”[J]. China Dairy, 2017(2):74 王玉庭. 农业部发布《全国苜蓿产业发展规划(2016—2020)》[J]. 中国乳业, 2017(2):74
ENDRE G, KERESZT A, KEVEI Z, et al. A receptor kinase gene regulating symbiotic nodule development[J]. Nature, 2002, 417(6892):962-966
STACEY G, LIBAULT M, BRECHENMACHER L, et al. Genetics and functional genomics of legume nodulation[J]. Current Opinion in Plant Biology, 2006, 9(2):110-121
HUANG S Y. Transcriptome analysis of alfalfa terrestris under drought stress and preliminary functional study of the aldehyde dehydrogenase gene MtALDH7A1[D]. Yangling:Northwest A & F University, 2019:1 黄思源. 干旱胁迫下蒺藜苜蓿的转录组分析及醛脱氢酶基因MtALDH7A1初步功能研究[D]. 杨凌:西北农林科技大学, 2019:1
WANG D, LI D, SUN P P, et al. Increment of extension-present situation and countermeasures of planting grass in saline-alkali land[J]. Agriculture and Technology, 2024, 44(17):1-4 王笛, 李达, 孙萍萍, 等. 拓面增量-盐碱地种草改良现状及对策[J]. 农业与技术, 2024, 44(17):1-4
LIANG C G, WANG Y, LI T, et al. Influence of over-expression of AngdhA gene on growth and nitrogen use efficiency of transgenic rice lines[J]. Journal of Plant Nutrition and Fertilizers, 2023, 29(6):1060-1069 梁成刚, 汪燕, 李天, 等. 过表达AngdhA对水稻生长与氮素利用效率的影响[J]. 植物营养与肥料学报, 2023, 29(6):1060-1069
XU Z L, LIU B, LI Y W, et al. Effects of NaCl stress at different concentrations on germination of onion seeds[J]. Shandong Agricultural Sciences, 2024, 56(8):74-79 徐子龙, 刘波, 李艳伟, 等. 不同浓度NaCl胁迫对洋葱种子发芽的影响[J]. 山东农业科学, 2024, 56(8):74-79
LI W H, WANG Z H, ZHENG X R, et al. Variation of soil salinity in wasteland of oasis saline drip irrigation area in Xinjiang[J]. Journal of Drainage and Irrigation Machinery Engineering, 2016, 34(8):722-729 李文昊, 王振华, 郑旭荣, 等. 新疆绿洲盐碱滴灌区域荒地土壤盐分变化[J]. 排灌机械工程学报, 2016, 34(8):722-729
HU L P, XIANG L X, ZHANG L, et al. The photoprotective role of spermidine in tomato seedlings under salinity-alkalinity stress[J]. PLoS One, 2014, 9(10):e110855
WU X H, CHEN X E. Physiological response of melon seedlings under the condition of mixed salt stress[J]. Journal of Qiqihar University (Natural Science Edition), 2015, 31(6):83-86 吴旭红, 陈晓娥. 混合盐碱胁迫条件下甜瓜幼苗的生理响应[J]. 齐齐哈尔大学学报(自然科学版), 2015, 31(6):83-86
ZULFIQAR F, NAFEES M, CHEN J J, et al. Chemical priming enhances plant tolerance to salt stress[J]. Frontiers in Plant Science, 2022, 13:946922
LIU X, FU L S, QIN P, et al. Overexpression of the wheat trehalose 6-phosphate synthase 11 gene enhances cold tolerance in Arabidopsis thaliana[J]. Gene, 2019, 710:210-217
GUO W L, CHEN J X, LIU L, et al. MsMIOX2, encoding a MsbZIP53-activated myo-inositol oxygenase, enhances saline-alkali stress tolerance by regulating cell wall pectin and hemicellulose biosynthesis in alfalfa[J]. The Plant Journal, 2024, 120(3):998-1013
LIU H Y. The function study of circadian clock gene MtLHY in medica[D]. Jinan:Shandong University, 2023:39 刘海洋. 生物钟基因MtLHY在蒺藜苜蓿盐胁迫中的功能研究[D]. 济南:山东大学, 2023:39
XU L, ZHANG L X, LIU Y J, et al. Overexpression of the elongation factor MtEF1A1 promotes salt stress tolerance in Arabidopsis thaliana and Medicago truncatula[J]. BMC Plant Biology, 2023, 23(1):138
STEPHAN A B, KUNZ H H, YANG E, et al. Rapid hyperosmotic-induced Ca²⁺ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters[J]. PNAS, 2016, 113(35):E5242-E5249
ZHANG Z, ZHANG H J, YAO T T, et al. The homeostasis of ions and reactive oxygen species in root and shoot play crucial roles in the tolerance of alfalfa to salt alkali stress[J]. Plant Physiology and Biochemistry, 2024, 216:109175
WANG R Y, WEN W W, ZHAO E H, et al. Cloning and salt-tolerance analysis of MsWRKY11 in alfalfa[J]. Acta Prataculturae Sinica, 2021, 30(11):157-169 王如月, 文武武, 赵恩华, 等. 紫花苜蓿MsWRKY11基因的克隆及其耐盐功能分析[J]. 草业学报, 2021, 30(11):157-169
LI X. Study on salt stress responsive mechanism of HD-Zip transcription factor MsHB7 in Medicago sativa L.[D]. Beijing:Chinese Academy of Agricultural Sciences, 2022:72-75 李霄. 紫花苜蓿HD-Zip转录因子MsHB7盐胁迫响应机制研究[D]. 北京:中国农业科学院, 2022:72-75
LI X. Study on the molecular mechanism of salt tolerance regulation by MsWRKY33 transcription factor in alfalfa[D]. Beijing:Chinese Academy of Agricultural Sciences, 2023:51 李心. 紫花苜蓿MsWRKY33转录因子调控耐盐性的分子机制研究[D]. 北京:中国农业科学院, 2023:51
RONG M R, YU R G, WEI Y M, et al. Excavation and expression analysis of NAC transcription factors related to salinity resistance of Medicago sativa[J]. Acta Agrestia Sinica, 2024, 32(4):1055-1067 荣梦茹, 余如刚, 韦英铭, 等. 紫花苜蓿耐盐性相关NAC转录因子的挖掘及表达分析[J]. 草地学报, 2024, 32(4):1055-1067
ZHANG A N, LIU Y, WANG F M, et al. Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene[J]. Molecular Breeding, 2019, 39:47
SLIMANI A, RAKLAMI A, OUFDOU K, et al. Isolation and characterization of PGPR and their potenzial for drought alleviation in barley plants[J]. Gesunde Pflanzen, 2023, 75(2):377-391
RAKLAMI A, OUFDOU K, TAHIRI A I, et al. Safe cultivation of Medicago sativa in metal-polluted soils from semi-arid regions assisted by heat- and metallo-resistant PGPR[J]. Microorganisms, 2019, 7(7):212
BECHTAOUI N, RAKLAMI A, TAHIRI A I, et al. Characterization of plant growth promoting rhizobacteria and their benefits on growth and phosphate nutrition of faba bean and wheat[J]. Biology Open, 2019, 8(7):bio043968
MEI T. Construction of a synthetic microbial community (SynComs) system for enhancing salt tolerance in alfalfa and its transcriptome analysis[D]. Hohhot:Inner Mongolia Agricultural University, 2023:27 梅亭. 合成菌群(SynComs)提高苜蓿耐盐性的体系构建及其转录组分析[D]. 呼和浩特:内蒙古农业大学, 2023:27
YU W Z, LUO L, QI X Y, et al. Insights into the impact of trans-Zeatin overproduction-engineered Sinorhizobium meliloti on alfalfa (Medicago sativa L.) tolerance to drought stress[J]. Journal of Agricultural and Food Chemistry, 2024, 72(15):8650-8663
LU J F. Study on the Physiological Mechanism of Alleviating Salt Stress in Alfalfa by Spraying Strigolactone[D].Yangzhou:Yangzhou University, 2024:47-52 卢俊峰. 喷施独脚金内酯缓解紫花苜蓿盐胁迫生理机制研究[D]. 扬州:扬州大学, 2024:47-52
CHEN J X, TANG L, GUO W L, et al. Oxalic acid secretion alleviates saline-alkali stress in alfalfa by improving photosynthetic characteristics and antioxidant activity[J]. Plant Physiology and Biochemistry, 2024, 208:108475
GUO X Q, SHI Y, ZHU G L, et al. Melatonin mitigated salinity stress on alfalfa by improving antioxidant defense and osmoregulation[J]. Agronomy, 2023, 13(7):1727
LI Q S. The regulatory effect of exogenous NO on the physiological traits of alfalfa under salt stress[D]. Hohhot:Inner Mongolia Agricultural University, 2023:24-39 李泉盛. 外源NO对盐胁迫下紫花苜蓿生理性状的调控效应[D]. 呼和浩特:内蒙古大学, 2023:24-39
ZHANG W H, XIE Y, WU Y, et al. Amelioration of salt-induced damage on alfalfa by exogenous application of silicon[J]. Grassland Science, 2022, 68(1):60-69
BAI J H, LU P N, LI F, et al. Metabolome and transcriptome analyses reveal the differences in the molecular mechanisms of oat leaves responding to salt and alkali stress conditions[J]. Agronomy, 2023, 13(6):1441
BAO Q H, WU Y, WANG Y, et al. Comparative transcriptomic analysis reveals transcriptional differences in the response of quinoa to salt and alkali stress responses[J]. Agronomy, 2024, 14(7):1596
YANG S, XU Y Q, TANG Z Z, et al. The impact of alkaline stress on plant growth and its alkaline resistance mechanisms[J]. International Journal of Molecular Sciences, 2024, 25(24):13719
XU M Z, XU Z P, LIU Y R, et al. Transcriptome analysis reveals the vital role of ABA plays in drought tolerance of the ABA-insensitive alfalfa (Medicago sativa L.)[J]. Agronomy, 2024, 14(3):406
WANG X J, YANG L L, GENG X, et al. Integrative analysis of metabolome and transcriptome reveals the different metabolite biosynthesis profiles related to palatability in winter and spring shoot in moso bamboo[J]. Plant Physiology and Biochemistry, 2023, 202:107973
HU L Y, ZHOU K, LIU Y, et al. Overexpression of MdMIPS1 enhances salt tolerance by improving osmosis, ion balance, and antioxidant activity in transgenic apple[J]. Plant Science, 2020, 301:110654
HOFFMANN R D, PORTES M T, OLSEN L I, et al. Plasma membrane H⁺-ATPases sustain pollen tube growth and fertilization[J]. Nature Communications, 2020, 11:2395
LISKO K A, TORRES R, HARRIS R S, et al. Elevating vitamin C content via overexpression of myo-inositol oxygenase and l-gulono-1, 4-lactone oxidase in Arabidopsis leads to enhanced biomass and tolerance to abiotic stresses[J]. In Vitro Cellular & Developmental Biology - Plant, 2013, 49(6):643-655
IM J H, KIM W C, HAN K H, et al. XERICO as a target for engineering stress-resilient crops: mechanisms, applications, and future directions[J]. Plant Physiology and Biochemistry, 2025, 225:110013
CHEN L, WANG J, QIU X, et al. Differences of physiological responses and transcriptional regulation of alfalfa with different drought tolerances under drought stresses[J]. Acta Agronomica Sinica, 2023, 49(8):2122-2132 陈力, 王靖, 邱晓, 等. 不同耐旱性紫花苜蓿干旱胁迫下生理响应和转录调控的差异研究[J]. 作物学报, 2023, 49(8):2122-2132
YAN X, HAN M T, LI S, et al. A member of NF-Y family, OsNF-YC5 negatively regulates salt tolerance in rice[J]. Gene, 2024, 892:147869
WANG Y M, JIAO P, WU C Y, et al. ZmNF-YB10, a maize NF-Y transcription factor, positively regulates drought and salt stress response in Arabidopsis thaliana[J]. GM Crops & Food, 2025, 16(1):28-45
HUANG S Y, HU T M, YANG P Z. Identification and function analysis of the PYL gene family in Medicago truncatula[J]. Pratacultural Science, 2019, 36(2):422-433 黄思源, 呼天明, 杨培志. 蒺藜苜蓿PYL基因家族的全基因组鉴定、表达和功能分析[J]. 草业科学, 2019, 36(2):422-433
AN Y L, MI X Z, XIA X B, et al. Genome-wide identification of the PYL gene family of tea plants (Camellia sinensis) revealed its expression profiles under different stress and tissues[J]. BMC Genomics, 2023, 24(1):362
DABRAVOLSKI S A, ISAYENKOV S V. The regulation of plant cell wall organisation under salt stress[J]. Frontiers in Plant Science, 2023, 14:1118313
BACHIR I H, PLOYET R, TEULIÈRES C, et al. Regulation of secondary cell wall lignification by abiotic and biotic constraints[M]//Lignin and hydroxycinnamic acids: biosynthesis and the buildup of the cell wall. Amsterdam:Elsevier, 2022:363-392
HOBAN S, ARCHER F I, BERTOLA L D, et al. Global genetic diversity status and trends: towards a suite of Essential Biodiversity Variables (EBVs) for genetic composition[J]. Biological Reviews, 2022, 97(4):1511-1538

2026年第34卷第7期

PDF下载

126

引用本文

BibTeX

文章信息

doi: 10.11733/j.issn.1007-0435.2026.07.007

接收时间：2025-09-05
首发时间：2026-09-23

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-09-05
修回日期：2025-10-29

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.07.007

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT