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2 Inner Mongolia Zhengshi Ecological Agriculture (Group) Co., Ltd., Hohhot, Inner Mongolia 010010, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309574786926346302, articleId=1309574784824999997, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=基于生理与转录组学的高加索三叶草耐寒性分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=本研究以耐寒性强的多年生牧草高加索三叶草（Trifolium ambiguum M. Bieb.）为材料，以20℃正常生长的幼苗为对照（CK），设置4℃低温胁迫2 h，6 h 和 12 h共3个处理，测定各时期高加索三叶草的生理指标，并对CK与胁迫12 h的植株进行转录组测序，解析其幼苗期在低温胁迫下的生理反应与适应机制。结果表明，4℃处理下，植株通过提高抗氧化酶（过氧化物酶、过氧化氢酶、超氧化物歧化酶）活性及渗透调节物质（可溶性蛋白、可溶性糖、游离脯氨酸）含量，有效清除活性氧，减轻了膜脂过氧化损伤，从而增强其抗寒能力。转录组分析显示，4℃处理12 h后共鉴定到15 428个差异表达基因，其中8909个基因上调，6519个基因下调。其中类黄酮生物合成通路与MAPK信号通路被显著激活：类黄酮通路上游关键基因查尔酮合成酶和查尔酮异构酶显著上调，促进了抗氧化物质（木犀草素、芹菜素、异黄酮）的合成；MAPK通路中MEKK1等基因的表达升高，表明低温信号通过磷酸化级联反应进行转导。其研究结果为解析高加索三叶草耐寒性提供了理论依据。, authors=吴倩¹, 赵雅茹², 曹克璠¹, 马一鸣¹, 索媛¹, 李舒宁¹, 齐海波¹, 王明玖¹, authorsList=吴倩, 赵雅茹, 曹克璠, 马一鸣, 索媛, 李舒宁, 齐海波, 王明玖, authorCompany=1 内蒙古农业大学草业学院/草地资源教育部重点实验室, 内蒙古 呼和浩特 010011;
2 内蒙古正时生态农业(集团)有限公司, 内蒙古 呼和浩特 010010, correspAuthors=null, authorNote=吴倩（1999-），女，汉族，内蒙古丰镇人，硕士研究生，主要从事草地资源生态与管理研究，E-mail:15548302887@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=6zPbTiMcTCnHMvAkAHQmgw==, pdfFileSize=3162709, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目“蒙农三叶草1号结瘤固氮机理及根瘤菌多样性研究” (31960353); “蒙农三叶草1号花期延长及其调控机理研究” (32160334))}, authors=null, keywords=[Keyword(id=1309574849601822892, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574784824999997, language=CN, orderNo=1, keyword=高加索三叶草), Keyword(id=1309574849660543149, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309574784824999997, language=CN, orderNo=2, 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