Article(id=1309573315233153247, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.05.004, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1749657600000, receivedDateStr=2025-06-12, revisedDate=1759939200000, revisedDateStr=2025-10-09, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156209046, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156209046, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156209046, creator=13701087609, updateTime=1790156209046, updator=13701087609, issue=Issue{id=1309573278520406630, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='5', pageStart='1557', pageEnd='1962', issueExtLink='null', onlineDate='null', pubDate='1778774400000', pubDateStr='2026-05-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156200293, creator='13701087609', updateTime=1790156821973, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575886110814336, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575886115008641, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1597, endPage=1609, ext={EN=ArticleExt(id=1309573318894780644, articleId=1309573315233153247, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Transcriptome Analysis of Galeruca daurica Larval Response to 20-Hydroxyecdysone, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Larvae of Galeruca daurica are the major pest that severely damages the grasslands of Inner Mongolia. To explore the molecular mechanisms by which 20-hydroxyecdysone （20E） regulates the development of its larval， transcriptome sequencing was conducted on 3rd-instar larvae treated with 20E for 48 hours by using the Illumina HiSeqTM 4000 platform. A total of 75 536 Unigenes were obtained， among which 40 161 were functionally annotated. Compared with the control group， a total of 500 differentially expressed genes （DEGs） were identified， including 258 upregulated and 242 downregulated ones. GO and KEGG analyses revealed that the DEGs were significantly enriched in terms/pathways such as ion transport， catalytic activity， longevity regulating pathway-multiple species， and FOXO signaling pathway. Furthermore， 11 ecdysone-related genes were screened out. The qRT-PCR verification results confirmed the reliability of the sequencing data. The results indicated that 20E may regulate the growth and development of G. daurica larvae by affecting multiple metabolic pathways. This study systematically analyzed the transcriptomic characteristics of G. daurica larvae in response to 20E regulation， providing a theoretical basis for clarifying its developmental mechanism and screening green control targets., authors=LIU Li1, GAO Li1, QILEMOGE1, GUO Ning1, XIN Ying1, LI Xiao-hai2, DUAN Tian-feng1, authorsList=LIU Li, GAO Li, QILEMOGE, GUO Ning, XIN Ying, LI Xiao-hai, DUAN Tian-feng, authorCompany=1 College of Ecology and Environment, Baotou Teachers College, Baotou, Inner Mongolia 010030, China; 2 Inner Mongolia Autonomous Region Civil Aviation Airport Group Co., Ltd. Baotou Branch, Baotou, Inner Mongolia 010040, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573318588596450, articleId=1309573315233153247, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=沙葱萤叶甲幼虫对蜕皮激素响应的转录组学分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=沙葱萤叶甲（Galeruca daurica）幼虫是严重危害内蒙古草原的主要害虫。为探究蜕皮激素（20-hydroxyecdysone，20E）调控其幼虫发育的分子机制，本研究采用Illumina HiseqTM 4000平台对20E处理48小时的3龄幼虫进行转录组测序。共获得75 536条Unigene，其中40 161条获得功能注释。与对照组相比，共获得500个差异表达基因（Differentially expressed genes，DEGs），其中258个上调，242个下调。GO与KEGG分析显示，差异基因显著富集于离子转运（Ion transport）、催化活性（Catalytic activity）、多物种长寿调控通路（Longevity regulating pathway-multiple species）及FOXO信号通路（FOXO signaling pathway）等。进一步筛选出11个蜕皮激素相关基因。qRT-PCR验证结果证实测序数据可靠。结果表明，20E可能通过影响多种代谢通路调控沙葱萤叶甲幼虫的生长发育。本研究首次系统解析了20E调控沙葱萤叶甲幼虫的转录组特征，为阐明其发育机制及绿色防控靶点筛选提供了理论依据。, authors=刘利1, 高丽1, 琪勒莫格1, 郭宁1, 邢颖1, 李晓海2, 段天凤1, authorsList=刘利, 高丽, 琪勒莫格, 郭宁, 邢颖, 李晓海, 段天凤, authorCompany=1 包头师范学院生态环境学院, 内蒙古 包头 010030; 2 内蒙古自治区民航机场集团有限责任公司 包头分公司, 内蒙古 包头 010040, correspAuthors=null, authorNote=刘利（1978-），男，蒙古族，内蒙古赤峰人，教授，博士，主要从事害虫生态治理研究，E-mail:liuli4304842@126.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=k1p/HViPnP+j+SGTzuNq1Q==, pdfFileSize=2323678, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=内蒙古自治区自然科学基金 (2023QN03059); 内蒙古自治区高等学校科学研究项目 (NJZZ23109); 包头师范学院高层次人才引进科研启动基金 (BTTCRCQD2023-013); 内蒙古自治区高校科技研究项目 (NJZY23005); 包头师范学院高水平研究成果培育自然科学类青年项目 (BSYKJ2023-ZQ02))}, authors=null, keywords=[Keyword(id=1309573319398097125, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573315233153247, language=CN, orderNo=1, keyword=沙葱萤叶甲), Keyword(id=1309573319788167399, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573315233153247, language=CN, orderNo=2, keyword=幼虫), Keyword(id=1309573320211792106, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573315233153247, language=CN, orderNo=3, keyword=蜕皮激素), Keyword(id=1309573320568307947, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573315233153247, language=CN, orderNo=4, keyword=转录组分析), Keyword(id=1309573322367664366, tenantId=1146029695717560320, journalId=1308397196074385509, 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detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.05.004, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.05.004, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.05.004, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1778774400000, fullTextJson=null, articleText=null, reference=YANG X K, HUANG D C, GE S Q, et al. Millions of acres of grassland in Inner Mongolia suffered from the outbreak of Galeruca daurica[J]. Chinese Bulletin of Entomology, 2010, 47(4):812 杨星科, 黄顶成, 葛斯琴, 等. 内蒙古百万亩草场遭受沙葱萤叶甲暴发危害[J]. 昆虫知识, 2010, 47(4):812 HAO X, ZHOU X R, PANG B P, et al. Effects of host plants on feeding amount, growth and development of Galeruca daurica joannis larvae(Coleoptera: Chrysomelidae)[J]. Acta Agrestia Sinica, 2014, 22(4):854-858 昊翔, 周晓榕, 庞保平, 等. 寄主植物对沙葱萤叶甲幼虫生长发育及取食的影响[J]. 草地学报, 2014, 22(4):854-858 MA C Y, WEI J, LI H S, et al. Preliminary studies on leaf beetle, Galeruca daurica on grassland[J]. Chinese Journal of Applied Entomology, 2012, 49(3):766-769 马崇勇, 伟军, 李海山, 等. 草原新害虫沙葱萤叶甲的初步研究[J]. 应用昆虫学报, 2012, 49(3):766-769 DUAN T F. Function of microRNAs in the regulation of reproductive diapause in Galeruca daurica[D]. Hohhot:Inner Mongolia Agricultural University, 2022:1 段天凤. MicroRNA在沙葱萤叶甲生殖滞育中的调控作用[D]. 呼和浩特:内蒙古农业大学, 2022:1 LIU H. Physiological function of BdTaiman in JH signaling pathway of Bactrocera dorsalis (Hendel)[D]. Chongqing:Southwest University, 2018:4 刘鸿. 桔小实蝇JH信号通路BdTaiman基因生理功能研究[D]. 重庆:西南大学, 2018:4 YAO T P, FORMAN B M, JIANG Z, et al. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes[J]. Nature, 1993, 366(6454):476-479 YAMANAKA N, ROMERO N M, MARTIN F A, et al. Neuroendocrine control of Drosophila larval light preference[J]. Science, 2013, 341(6150):1113-1116 YU J. 20-hydroxyecdysone regulates pupation of Apis mellifera ligustica larvae through nuclear receptor ECR and transcription factor FoxO[D]. Taian:Shandong Agricultural University, 2023:7-16 于静. 20-羟基蜕皮酮通过核受体ECR和转录因子FoxO调控意大利蜜蜂幼虫化蛹的研究[D]. 泰安:山东农业大学, 2023:7-16 YAMANAKA N. Ecdysteroid signalling in insects-From biosynthesis to gene expression regulation[J]. Advances in Insect Physiology, 2021, 60:1-36 GONG Y W, SUN Y J, KUANG S J, et al. 20-Hydroxyecdysone regulates pupal fatty acid metabolism, maintaining lipid metabolic homeostasis in Chilo suppressalis[EB/OL]. https://doi.org/10.1111/1744-7917.70053, 2025-04-18/2025-06-11 HOSSAIN M S, LIU Y, ZHOU S, et al. 20-Hydroxyecdysone-induced transcriptional activity of FoxO upregulates brummer and acid lipase-1 and promotes lipolysis in Bombyx fat body[J]. Insect Biochemistry and Molecular Biology, 2013, 43(9):829-838 JI C H, ZHANG N, JIANG H, et al. 20-hydroxyecdysone regulates expression of methionine sulfoxide reductases through transcription factor FOXO in the red flour beetle, Tribolium castaneum[J]. Insect Biochemistry and Molecular Biology, 2021, 131:103546 HUANG S H, YANG H H, CHEN X X, et al. Genomic transcriptional response to 20-hydroxyecdysone in the fat body of silkworm, Bombyx mori[J]. Gene Reports, 2018, 13:134-140 YAO Z H, LI L, PANG B P, et al. Transcriptome analysis of the adult Galeruca daurica (Coleoptera: Chrysomelidae) in response to 20-hydroxyecdysone[J]. Journal of Environmental Entomology, 2023, 45(1):155-162 姚知含, 李玲, 庞保平, 等. 沙葱萤叶甲对蜕皮激素响应的转录组分析[J]. 环境昆虫学报, 2023, 45(1):155-162 TAN Y, ZHOU X R, PANG B P. Reference gene selection and evaluation for expression analysis using qRT-PCR in Galeruca daurica (Joannis)[J]. Bulletin of Entomological Research, 2017, 107(3):359-368 LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4):402-408 CAI M J, ZHAO W L, JING Y P, et al. 20-Hydroxyecdysone activates Forkhead box O to promote proteolysis during Helicoverpa armigera molting[J]. Development, 2016, 143(6):1005-1015 VAN DER HORST A, BURGERING B M T. Stressing the role of FoxO proteins in lifespan and disease[J]. Nature Reviews Molecular Cell Biology, 2007, 8(6):440-450 MARTINS R, LITHGOW G J, LINK W. Long live FOXO: unraveling the role of FOXO proteins in aging and longevity[J]. Aging Cell, 2016, 15(2):196-207 SONG J B, LI Z Q, ZHOU L, et al. FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species[J]. Nature Communications, 2024, 15:7144 DEBÈS C, PAPADAKIS A, GRÖNKE S, et al. Ageing-associated changes in transcriptional elongation influence longevity[J]. Nature, 2023, 616(7958):814-821 YUAN D W, ZHOU S, LIU S N, et al. The AMPK-PP2A axis in insect fat body is activated by 20-hydroxyecdysone to antagonize insulin/IGF signaling and restrict growth rate[J]. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(17):9292-9301 ZHAO J B, LIU Y X, HUANG H T, et al. Cross-talking of mTOR and AMPK signaling pathway in nutrient metabolism of animal cells[J]. China Feed, 2023(17):1-6 赵金波, 刘盈序, 黄合特, 等. AMPK/mTOR信号通路交叉调控在动物细胞营养代谢的作用[J]. 中国饲料, 2023(17):1-6 QU M B, SUN S P, LIU Y S, et al. Insect group II chitinase OfChtII promotes chitin degradation during larva–Pupa molting[J]. Insect Science, 2021, 28(3):692-704 ZHANG X, WANG Y, ZHANG S F, et al. RNAi-mediated silencing of the chitinase 5 gene for fall webworm (Hyphantria cunea) can inhibit larval molting depending on the timing of dsRNA injection[J]. Insects, 2021, 12(5):406 ZHAO D, LIU Z R, WU H, et al. RNA interference-mediated functional characterization of Group I chitin deacetylases in Holotrichia parallela Motschulsky[J]. Pesticide Biochemistry and Physiology, 2021, 173:104770 LU J B, GUO J S, CHEN X, et al. Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens[J]. Insect Science, 2022, 29(2):363-378 NIWA R, NIWA Y S. Enzymes for ecdysteroid biosynthesis: their biological functions in insects and beyond[J]. Bioscience, Biotechnology, and Biochemistry, 2014, 78(8):1283-1292 ONO H, MORITA S, ASAKURA I, et al. Conversion of 3-oxo steroids into ecdysteroids triggers molting and expression of 20E-inducible genes in Drosophila melanogaster[J]. Biochemical and Biophysical Research Communications, 2012, 421(3):561-566 MARCHAL E, BADISCO L, VERLINDEN H, et al. Role of the Halloween genes, Spook and Phantom in ecdysteroidogenesis in the desert locust, Schistocerca gregaria[J]. Journal of Insect Physiology, 2011, 57(9):1240-1248 RUSTEN T E, LINDMO K, JUHÁSZ G, et al. Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[J]. Developmental Cell, 2004, 7(2):179-192 COLOMBANI J, BIANCHINI L, LAYALLE S, et al. Antagonistic actions of ecdysone and insulins determine final size in Drosophila[J]. Science, 2005, 310(5748):667-670 KESHAN B, THOUNAOJAM B, KH S D. Insulin and 20-hydroxyecdysone action in Bombyx mori: glycogen content and expression pattern of insulin and ecdysone receptors in fat body[J]. General and Comparative Endocrinology, 2017, 241:108-117 LI Y X, SHAO B Y, HOU M Y, et al. Succinylation enables IDE to act as a hub of larval tissue destruction and adult tissue reconstruction during insect metamorphosis[J]. Science Advances, 2025, 11(6):eads0643 LI Y L. Regulation of G protein-coupled receptors by 20- hydroxyecdysone and promotion of insect metamorphosis through insulin pathway[D]. Jinan:Shandong University, 2021:46-74 李艳丽. 20-羟基蜕皮激素调控G蛋白偶联受体及胰岛素途径促进昆虫变态发育的研究[D]. 济南:山东大学, 2021:46-74 CHEN W W, CHEN L E, LI D, et al. Two alternative splicing variants of a sugar gustatory receptor modulate fecundity through different signalling pathways in the brown planthopper, Nilaparvata lugens[J]. Journal of Insect Physiology, 2019, 119:103966 DENLINGER D L. Regulation of diapause[J]. Annual Review of Entomology, 2002, 47:93-122)
Larvae of Galeruca daurica are the major pest that severely damages the grasslands of Inner Mongolia. To explore the molecular mechanisms by which 20-hydroxyecdysone （20E） regulates the development of its larval， transcriptome sequencing was conducted on 3rd-instar larvae treated with 20E for 48 hours by using the Illumina HiSeqTM 4000 platform. A total of 75 536 Unigenes were obtained， among which 40 161 were functionally annotated. Compared with the control group， a total of 500 differentially expressed genes （DEGs） were identified， including 258 upregulated and 242 downregulated ones. GO and KEGG analyses revealed that the DEGs were significantly enriched in terms/pathways such as ion transport， catalytic activity， longevity regulating pathway-multiple species， and FOXO signaling pathway. Furthermore， 11 ecdysone-related genes were screened out. The qRT-PCR verification results confirmed the reliability of the sequencing data. The results indicated that 20E may regulate the growth and development of G. daurica larvae by affecting multiple metabolic pathways. This study systematically analyzed the transcriptomic characteristics of G. daurica larvae in response to 20E regulation， providing a theoretical basis for clarifying its developmental mechanism and screening green control targets.
YANG X K, HUANG D C, GE S Q, et al. Millions of acres of grassland in Inner Mongolia suffered from the outbreak of Galeruca daurica[J]. Chinese Bulletin of Entomology, 2010, 47(4):812 杨星科, 黄顶成, 葛斯琴, 等. 内蒙古百万亩草场遭受沙葱萤叶甲暴发危害[J]. 昆虫知识, 2010, 47(4):812 HAO X, ZHOU X R, PANG B P, et al. Effects of host plants on feeding amount, growth and development of Galeruca daurica joannis larvae(Coleoptera: Chrysomelidae)[J]. Acta Agrestia Sinica, 2014, 22(4):854-858 昊翔, 周晓榕, 庞保平, 等. 寄主植物对沙葱萤叶甲幼虫生长发育及取食的影响[J]. 草地学报, 2014, 22(4):854-858 MA C Y, WEI J, LI H S, et al. Preliminary studies on leaf beetle, Galeruca daurica on grassland[J]. Chinese Journal of Applied Entomology, 2012, 49(3):766-769 马崇勇, 伟军, 李海山, 等. 草原新害虫沙葱萤叶甲的初步研究[J]. 应用昆虫学报, 2012, 49(3):766-769 DUAN T F. Function of microRNAs in the regulation of reproductive diapause in Galeruca daurica[D]. Hohhot:Inner Mongolia Agricultural University, 2022:1 段天凤. MicroRNA在沙葱萤叶甲生殖滞育中的调控作用[D]. 呼和浩特:内蒙古农业大学, 2022:1 LIU H. Physiological function of BdTaiman in JH signaling pathway of Bactrocera dorsalis (Hendel)[D]. Chongqing:Southwest University, 2018:4 刘鸿. 桔小实蝇JH信号通路BdTaiman基因生理功能研究[D]. 重庆:西南大学, 2018:4 YAO T P, FORMAN B M, JIANG Z, et al. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes[J]. Nature, 1993, 366(6454):476-479 YAMANAKA N, ROMERO N M, MARTIN F A, et al. Neuroendocrine control of Drosophila larval light preference[J]. Science, 2013, 341(6150):1113-1116 YU J. 20-hydroxyecdysone regulates pupation of Apis mellifera ligustica larvae through nuclear receptor ECR and transcription factor FoxO[D]. Taian:Shandong Agricultural University, 2023:7-16 于静. 20-羟基蜕皮酮通过核受体ECR和转录因子FoxO调控意大利蜜蜂幼虫化蛹的研究[D]. 泰安:山东农业大学, 2023:7-16 YAMANAKA N. Ecdysteroid signalling in insects-From biosynthesis to gene expression regulation[J]. Advances in Insect Physiology, 2021, 60:1-36 GONG Y W, SUN Y J, KUANG S J, et al. 20-Hydroxyecdysone regulates pupal fatty acid metabolism, maintaining lipid metabolic homeostasis in Chilo suppressalis[EB/OL]. https://doi.org/10.1111/1744-7917.70053, 2025-04-18/2025-06-11 HOSSAIN M S, LIU Y, ZHOU S, et al. 20-Hydroxyecdysone-induced transcriptional activity of FoxO upregulates brummer and acid lipase-1 and promotes lipolysis in Bombyx fat body[J]. Insect Biochemistry and Molecular Biology, 2013, 43(9):829-838 JI C H, ZHANG N, JIANG H, et al. 20-hydroxyecdysone regulates expression of methionine sulfoxide reductases through transcription factor FOXO in the red flour beetle, Tribolium castaneum[J]. Insect Biochemistry and Molecular Biology, 2021, 131:103546 HUANG S H, YANG H H, CHEN X X, et al. Genomic transcriptional response to 20-hydroxyecdysone in the fat body of silkworm, Bombyx mori[J]. Gene Reports, 2018, 13:134-140 YAO Z H, LI L, PANG B P, et al. Transcriptome analysis of the adult Galeruca daurica (Coleoptera: Chrysomelidae) in response to 20-hydroxyecdysone[J]. Journal of Environmental Entomology, 2023, 45(1):155-162 姚知含, 李玲, 庞保平, 等. 沙葱萤叶甲对蜕皮激素响应的转录组分析[J]. 环境昆虫学报, 2023, 45(1):155-162 TAN Y, ZHOU X R, PANG B P. Reference gene selection and evaluation for expression analysis using qRT-PCR in Galeruca daurica (Joannis)[J]. Bulletin of Entomological Research, 2017, 107(3):359-368 LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4):402-408 CAI M J, ZHAO W L, JING Y P, et al. 20-Hydroxyecdysone activates Forkhead box O to promote proteolysis during Helicoverpa armigera molting[J]. Development, 2016, 143(6):1005-1015 VAN DER HORST A, BURGERING B M T. Stressing the role of FoxO proteins in lifespan and disease[J]. Nature Reviews Molecular Cell Biology, 2007, 8(6):440-450 MARTINS R, LITHGOW G J, LINK W. Long live FOXO: unraveling the role of FOXO proteins in aging and longevity[J]. Aging Cell, 2016, 15(2):196-207 SONG J B, LI Z Q, ZHOU L, et al. FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species[J]. Nature Communications, 2024, 15:7144 DEBÈS C, PAPADAKIS A, GRÖNKE S, et al. Ageing-associated changes in transcriptional elongation influence longevity[J]. Nature, 2023, 616(7958):814-821 YUAN D W, ZHOU S, LIU S N, et al. The AMPK-PP2A axis in insect fat body is activated by 20-hydroxyecdysone to antagonize insulin/IGF signaling and restrict growth rate[J]. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(17):9292-9301 ZHAO J B, LIU Y X, HUANG H T, et al. Cross-talking of mTOR and AMPK signaling pathway in nutrient metabolism of animal cells[J]. China Feed, 2023(17):1-6 赵金波, 刘盈序, 黄合特, 等. AMPK/mTOR信号通路交叉调控在动物细胞营养代谢的作用[J]. 中国饲料, 2023(17):1-6 QU M B, SUN S P, LIU Y S, et al. Insect group II chitinase OfChtII promotes chitin degradation during larva–Pupa molting[J]. Insect Science, 2021, 28(3):692-704 ZHANG X, WANG Y, ZHANG S F, et al. RNAi-mediated silencing of the chitinase 5 gene for fall webworm (Hyphantria cunea) can inhibit larval molting depending on the timing of dsRNA injection[J]. Insects, 2021, 12(5):406 ZHAO D, LIU Z R, WU H, et al. RNA interference-mediated functional characterization of Group I chitin deacetylases in Holotrichia parallela Motschulsky[J]. Pesticide Biochemistry and Physiology, 2021, 173:104770 LU J B, GUO J S, CHEN X, et al. Chitin synthase 1 and five cuticle protein genes are involved in serosal cuticle formation during early embryogenesis to enhance eggshells in Nilaparvata lugens[J]. Insect Science, 2022, 29(2):363-378 NIWA R, NIWA Y S. Enzymes for ecdysteroid biosynthesis: their biological functions in insects and beyond[J]. Bioscience, Biotechnology, and Biochemistry, 2014, 78(8):1283-1292 ONO H, MORITA S, ASAKURA I, et al. Conversion of 3-oxo steroids into ecdysteroids triggers molting and expression of 20E-inducible genes in Drosophila melanogaster[J]. Biochemical and Biophysical Research Communications, 2012, 421(3):561-566 MARCHAL E, BADISCO L, VERLINDEN H, et al. Role of the Halloween genes, Spook and Phantom in ecdysteroidogenesis in the desert locust, Schistocerca gregaria[J]. Journal of Insect Physiology, 2011, 57(9):1240-1248 RUSTEN T E, LINDMO K, JUHÁSZ G, et al. Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway[J]. Developmental Cell, 2004, 7(2):179-192 COLOMBANI J, BIANCHINI L, LAYALLE S, et al. Antagonistic actions of ecdysone and insulins determine final size in Drosophila[J]. Science, 2005, 310(5748):667-670 KESHAN B, THOUNAOJAM B, KH S D. Insulin and 20-hydroxyecdysone action in Bombyx mori: glycogen content and expression pattern of insulin and ecdysone receptors in fat body[J]. General and Comparative Endocrinology, 2017, 241:108-117 LI Y X, SHAO B Y, HOU M Y, et al. Succinylation enables IDE to act as a hub of larval tissue destruction and adult tissue reconstruction during insect metamorphosis[J]. Science Advances, 2025, 11(6):eads0643 LI Y L. Regulation of G protein-coupled receptors by 20- hydroxyecdysone and promotion of insect metamorphosis through insulin pathway[D]. Jinan:Shandong University, 2021:46-74 李艳丽. 20-羟基蜕皮激素调控G蛋白偶联受体及胰岛素途径促进昆虫变态发育的研究[D]. 济南:山东大学, 2021:46-74 CHEN W W, CHEN L E, LI D, et al. Two alternative splicing variants of a sugar gustatory receptor modulate fecundity through different signalling pathways in the brown planthopper, Nilaparvata lugens[J]. Journal of Insect Physiology, 2019, 119:103966 DENLINGER D L. Regulation of diapause[J]. Annual Review of Entomology, 2002, 47:93-122
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