Comprehensive Evaluation of Drought Tolerance in Bromus inermis Germplasm at the Germination Stage
HUANG Meng-qi1, LI Chen-jian1, WANG Yu-xiang1, LU Lu1, WANG Qing-yu1, JIANG Yiu1, TONG Ai-xiang2
Affiliations
1 Key Laboratory of Grassland Resources and Ecology of Western Arid Region Ministry of Education, Key Laboratory of Grassland Resources and Ecology of Xinjiang, College of Grassland Science Xinjiang Agricultural University, Urumqi, Xinjiang Province 830052, China; 2 Beijing Academy of Science and Technology, Beijing 10093, China
Drought is one of the primary stress factors affecting plant production, and the germination stage is the most vulnerable period in the plant life cycle. In the breeding of drought-resistant Bromus inermis, evaluating drought tolerance during the germination stage is particularly important. This study employed the double-layer filter paper germination method, measuring eight indicators-including germination rate, germination potential, germination index, root length, and shoot length-under 25% PEG-6000 stress for 100 B. inermis germplasm resources. Principal component analysis, membership functions, and hierarchical clustering analysis were used to comprehensively assess drought tolerance during the germination stage. The results showed that, compared to the control, all observed indicators decreased under 25% PEG stress, with coefficient of variation ranging from 20.853% to 97.876%. There was a significant correlation (P<0.05) among most experimental indicators. There were substantial differences in drought tolerance among materials, with the comprehensive drought tolerance coefficient ranging from 0.612 to 0.169 and the D-value ranging from 0.861 to 0.068. Cluster analysis classified the 100 t100 Bromus inermis germplasm resources into four groups: highly drought-resistant (14 materials), moderately drought-resistant (34 materials), weakly drought-resistant (45 materials), and drought-sensitive (7 materials). This study identified drought-resistant germplasm such as W1Z622, W1Z627, and W1Z595. The research provides high-quality materials and theoretical support for drought-resistant breeding of B. inermis.
HUANG Meng-qi, LI Chen-jian, WANG Yu-xiang, LU Lu, WANG Qing-yu, JIANG Yiu, TONG Ai-xiang.
Comprehensive Evaluation of Drought Tolerance in Bromus inermis Germplasm at the Germination Stage[J].
Acta Agrestia Sinica,
2026
, 34
(5)
: 1677
-1690
.
DOI: 10.11733/j.issn.1007-0435.2026.05.011
基金
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财政部和农业农村部:国家现代农业产业技术体系牧草产业技术体系 (CARS-34)
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