Article(id=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.05.010, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750953600000, receivedDateStr=2025-06-27, revisedDate=1760976000000, revisedDateStr=2025-10-21, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156206543, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156206543, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156206543, creator=13701087609, updateTime=1790156206543, updator=13701087609, issue=Issue{id=1309573278520406630, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='5', pageStart='1557', pageEnd='1962', issueExtLink='null', onlineDate='null', pubDate='1778774400000', pubDateStr='2026-05-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156200293, creator='13701087609', updateTime=1790156821973, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575886110814336, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575886115008641, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573278520406630, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1664, endPage=1676, ext={EN=ArticleExt(id=1309573305078735249, articleId=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To evaluate the differences in salt tolerance among different alfalfa (Medicago sativa L.) cultivarsunder salt stress conditions and screen high-quality salt-tolerant germplasm resources,this study used 25 alfalfa cultivars as experimental materials, with two treatments of salt stress (200 mmol·L-1 NaCl solution)and control (ultrapure water). Germination and growth parameters were measured during the germination period (eight indicators, such as germination energy and germination rate, etc.) and seedling stage (six indicators, such as plant height and branch number, etc.), and a comprehensive evaluation of salt tolerance was conducted using methods such as membership function value, comprehensive salt tolerance (D value), and cluster analyses. The results showed that salt stress significantly reduced all germination parameters, with the seed vigor index exhibiting the highest reduction rate (-92.26%), whereas the seedling dry weight was the least affected (-11.76%). Cluster analysis classified the 25 alfalfa cultivars into five salt tolerance grades at the germination stage: included 2 of the strongest salt-tolerant cultivars, 4 strong salt-tolerant cultivars, 5 moderate salt-tolerant cultivars, 10 weak salt-resistance cultivars, and 4 salt-sensitive cultivars. Further evaluation of salt tolerance of 12 representative alfalfa cultivars during the seedling stage revealed salt stress had extremely significant effects on the plant height, above-ground fresh weight, relative water content, and electrical conductivity of alfalfa (P<0.01), with significant differences between cultivars. By integrating the indicators of both germination and seedling stages, the three strongest salt-tolerant cultivars, namely ‘Xinmu No.4’‘Zhongmu No.4’, and ‘Bara520YQ’, were ultimately selected as important germplasm resources for salt-tolerant breeding and cultivation in saline-alkali soil regions., authors=SU Li-he1, HUANG Rong-zheng1, HU Tian-yu1, MA Chun-hui1, authorsList=SU Li-he, HUANG Rong-zheng, HU Tian-yu, MA Chun-hui, authorCompany=1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573304999043472, articleId=1309573304730608015, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=盐胁迫下不同紫花苜蓿品种萌发期和苗期的耐盐性综合评价, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为评价不同紫花苜蓿(Medicago sativa)品种盐胁迫下的耐盐性差异,筛选优质耐盐种质资源。本研究以25份苜蓿品种为材料,设置200 mmol·L⁻¹ NaCl盐胁迫和超纯水对照处理,测定萌发期(发芽势、发芽率等8项)及苗期(株高、分枝数等6项)参数,采用隶属函数、综合耐盐值D及聚类分析进行综合评价。结果显示:盐胁迫显著降低所有萌发参数,种子活力指数降幅最大(-92.26%),幼苗干重受影响最小(-11.76%);聚类分析将萌发期25份材料划分为5个耐盐等级,含2份强耐盐、4份较强耐盐、5份中等耐盐、10份弱耐盐及4份盐敏感品种。对12份代表性品种苗期评价发现,盐胁迫对株高、地上鲜重、叶片相对含水量及电导率影响极显著(P<0.01),且品种间差异显著。整合萌发期与苗期指标,最终筛选出‘新牧4号’‘中苜4号’和‘Bara520YQ’3个强耐盐品种,可作为耐盐育种及盐碱地栽培的重要种质资源。, authors=苏力合1, 黄嵘峥1, 胡天宇1, 马春晖1, authorsList=苏力合, 黄嵘峥, 胡天宇, 马春晖, authorCompany=1 石河子大学动物科技学院, 新疆 石河子 832000, correspAuthors=null, authorNote=苏力合(1995-),女,蒙古族,新疆塔城人,博士,主要从事牧草生理及分子机制研究,E-mail:lihesu2025@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, 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ZHU C C, SHI K, HE Q K, et al. Effects of mixed saline-alkali stress on physiology and gene expression of alfalfa seedlings[J]. Acta Agrestia Sinica, 2024, 32(4):1044-1054 朱晨晨, 史昆, 何沁坤, 等. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4):1044-1054
CHEN C J, MA L, BAO M F, et al. Identification and evaluation of drought resistance for 111 germplasm resources of alfalfa during germination stage[J]. Scientia Agricultura Sinica, 2025, 58(10):1896-1907 陈彩锦, 马琳, 包明芳, 等. 111份紫花苜蓿种质资源萌发期抗旱性鉴定评价[J]. 中国农业科学, 2025, 58(10):1896-1907
E J F, YANG S Q, ZHANG W F, et al. Effect of forage planting on improvement of saline soil under subsurface pipe in agro-pastoral intertwined zones[J]. Transactions of the Chinese Society for Agricultural Machinery, 2024, 55(9):402-410 鄂继芳, 杨树青, 张万锋, 等. 暗管条件下牧草种植对农牧交错区盐渍土的改土效应[J]. 农业机械学报, 2024, 55(9):402-410
LV Y Q, HU J, GAO D X, et al. Effects of forage cultivation and cattle manure application on nutrient and humus composition of saline alkali soil in the Songnen Plain[J]. Journal of Agricultural Resources and Environment, 2025, 42(1):169-176 吕悦齐, 胡娟, 高东雪, 等. 饲草种植及牛粪施用对松嫩平原盐碱土养分及腐殖质组成的影响[J]. 农业资源与环境学报, 2025, 42(1):169-176
SHI J Q, XIE N, CUI S Q, et al. Evaluation of salt tolerance in 16 alfalfa varieties during germination under different salt concentration stress[J]. Acta Agrestia Sinica, 2025, 33(2):472-480 石嘉琦, 谢楠, 崔素倩, 等. 不同盐浓度胁迫下16个紫花苜蓿品种萌发期耐盐性评价[J]. 草地学报, 2025, 33(2):472-480
ZHU K, LIU H J, FENG C L, et al. Comprehensive evaluation on the salt tolerance of seed germination of different alfalfa varieties under salt stress[J]. Acta Agrestia Sinica, 2023, 31(12):3724-3733 朱琨, 刘骅峻, 冯成龙, 等. 盐胁迫对不同苜蓿品种种子萌发的耐盐性综合评价[J]. 草地学报, 2023, 31(12):3724-3733
WU M M, XU J Y, NIE Z J, et al. Physiological, biochemical and transcriptomic insights into the mechanisms by which molybdenum mitigates cadmium toxicity in Triticum aestivum L[J]. Journal of Hazardous Materials, 2024, 472:134516
ZHANG X Y, LI S H, TANG T, et al. Comparison of morphological, physiological, and related-gene expression responses to saline-alkali stress in eight apple rootstock genotypes[J]. Scientia Horticulturae, 2022, 306:111455
WANG M, DING Y, WANG Q, et al. NaCl treatment on physio-biochemical metabolism and phenolics accumulation in barley seedlings[J]. Food chemistry, 2020, 331:127282
LI H, LIU L, HUANG Q, et al. Identification and screening of salt tolerance of cotton germplasm resources at germination stage[J]. Acta Agronomica Sinica, 2024, 50(5):1147-1157 李航, 刘丽, 黄乾, 等. 棉花种质资源萌发期耐盐性鉴定及筛选[J]. 作物学报, 2024, 50(5):1147-1157
WANG Y L, WEI L, LI N, et al. Comprehensive evaluation of salt tolerance of three forage seed varieties during germination[J]. Pratacultural Science, 2023, 40(12):3104-3113 王娅琳, 魏琳, 李娜, 等. 3种牧草种子萌发期耐盐性综合评价[J]. 草业科学, 2023, 40(12):3104-3113
YANG X J, SUN Q Y, ZHANG Z, et al. Comprehensive evaluation on salt tolerance of 61 alfalfa cultivars during seed germination[J]. Acta Agrestia Sinica, 2025, 33(4):1181-1191 姚兴洁, 孙庆赢, 张昭, 等. 61个品种紫花苜蓿种子萌发期耐盐性综合评价[J]. 草地学报, 2025, 33(4):1181-1191
ZHANG Z Y, LI X, WANG Y, et al. Salt tolerance evaluation and salt tolerance index screening of 59 alfalfa germplasm materials at seedling stage[J]. Acta Agrestia Sinica, 2020, 28(1):112-121 张则宇, 李雪, 王焱, 等. 59份苜蓿种质材料苗期耐盐性评价及耐盐指标筛选[J]. 草地学报, 2020, 28(1):112-121
WANG Y N, PENG X Y, HUA X Y, et al. Research progress on the physiological function of plant cotyledons[J]. Pratacultural Science, 2018, 35(12):2988-2997 王英男, 彭晓媛, 华晓雨, 等. 植物子叶生理功能的研究进展[J]. 草业科学, 2018, 35(12):2988-2997
ZHANG X P, DENG Y T, YANG R, et al. Evaluation of salt tolerance of forage oat germplasm resources at germination and seedling stages[J]. Acta Agrestia Sinica, 2025, 33(1):3741-3755 张晓澎, 邓焱天, 杨蕊, 等. 饲用燕麦种质资源萌发期及苗期耐盐性鉴定评价[J]. 草地学报, 2025, 33(1):3741-3755
YAN J H, LI X C, SONG W X, et al. Evaluation of salt tolerance of 57 Bromus inermis germplasm at the germination and seedling stages[J].https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html 闫聚辉, 李小聪, 宋文学, 等. 57份无芒雀麦种质萌发期与苗期耐盐性评价[J/OL]. https://kns.cnki.net/kcms/detail/62.1069.S.20250528.1345.002.html
XIONG X, GUI W Y, LIU M H, et al. Evaluation of salt tolerance in different alfalfa varieties under uniform and non-uniform salt stress[J]. Acta Prataculturae Sinica, 2018, 27(9):67-76 熊雪, 桂维阳, 刘沫含, 等. 不同紫花苜蓿品种在均匀与不均匀盐胁迫下的耐盐性评价[J]. 草业学报, 2018, 27(9):67-76
LÜ Y Z, Y X J, ZHANG Z, et al. Evaluation of alkali tolerance and key indicators screening during seed germination of different alfalfa cultivars[J]. Acta Agrestia Sinica, 2025, 33(6):1852-1861 吕艳贞, 姚兴洁, 张昭, 等. 不同品种紫花苜蓿种子萌发期耐碱性评价与关键指标筛选[J]. 草地学报, 2025, 33(6):1852-1861)
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草地学报 |研究论文 2026 , 34 (5) : 1664 -1676
盐胁迫下不同紫花苜蓿品种萌发期和苗期的耐盐性综合评价
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苏力合1, 黄嵘峥1, 胡天宇1, 马春晖1
作者信息
    1 石河子大学动物科技学院, 新疆 石河子 832000
作者简介:
苏力合(1995-),女,蒙古族,新疆塔城人,博士,主要从事牧草生理及分子机制研究,E-mail:lihesu2025@163.com
Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress
  • SU Li-he1, HUANG Rong-zheng1, HU Tian-yu1, MA Chun-hui1
  • Affiliations
      1 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China
    doi: 10.11733/j.issn.1007-0435.2026.05.010
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    为评价不同紫花苜蓿(Medicago sativa)品种盐胁迫下的耐盐性差异,筛选优质耐盐种质资源。本研究以25份苜蓿品种为材料,设置200 mmol·L⁻¹ NaCl盐胁迫和超纯水对照处理,测定萌发期(发芽势、发芽率等8项)及苗期(株高、分枝数等6项)参数,采用隶属函数、综合耐盐值D及聚类分析进行综合评价。结果显示:盐胁迫显著降低所有萌发参数,种子活力指数降幅最大(-92.26%),幼苗干重受影响最小(-11.76%);聚类分析将萌发期25份材料划分为5个耐盐等级,含2份强耐盐、4份较强耐盐、5份中等耐盐、10份弱耐盐及4份盐敏感品种。对12份代表性品种苗期评价发现,盐胁迫对株高、地上鲜重、叶片相对含水量及电导率影响极显著(P<0.01),且品种间差异显著。整合萌发期与苗期指标,最终筛选出‘新牧4号’‘中苜4号’和‘Bara520YQ’3个强耐盐品种,可作为耐盐育种及盐碱地栽培的重要种质资源。
    紫花苜蓿  /  种子萌发  /  苗期  /  耐盐性  /  综合评价
    To evaluate the differences in salt tolerance among different alfalfa (Medicago sativa L.) cultivarsunder salt stress conditions and screen high-quality salt-tolerant germplasm resources,this study used 25 alfalfa cultivars as experimental materials, with two treatments of salt stress (200 mmol·L-1 NaCl solution)and control (ultrapure water). Germination and growth parameters were measured during the germination period (eight indicators, such as germination energy and germination rate, etc.) and seedling stage (six indicators, such as plant height and branch number, etc.), and a comprehensive evaluation of salt tolerance was conducted using methods such as membership function value, comprehensive salt tolerance (D value), and cluster analyses. The results showed that salt stress significantly reduced all germination parameters, with the seed vigor index exhibiting the highest reduction rate (-92.26%), whereas the seedling dry weight was the least affected (-11.76%). Cluster analysis classified the 25 alfalfa cultivars into five salt tolerance grades at the germination stage: included 2 of the strongest salt-tolerant cultivars, 4 strong salt-tolerant cultivars, 5 moderate salt-tolerant cultivars, 10 weak salt-resistance cultivars, and 4 salt-sensitive cultivars. Further evaluation of salt tolerance of 12 representative alfalfa cultivars during the seedling stage revealed salt stress had extremely significant effects on the plant height, above-ground fresh weight, relative water content, and electrical conductivity of alfalfa (P<0.01), with significant differences between cultivars. By integrating the indicators of both germination and seedling stages, the three strongest salt-tolerant cultivars, namely ‘Xinmu No.4’‘Zhongmu No.4’, and ‘Bara520YQ’, were ultimately selected as important germplasm resources for salt-tolerant breeding and cultivation in saline-alkali soil regions.
    Medicago sativa L.  /  Seed germination  /  Seedling stages  /  Salt tolerance  /  Comprehensive evaluation
    苏力合, 黄嵘峥, 胡天宇, 马春晖. 盐胁迫下不同紫花苜蓿品种萌发期和苗期的耐盐性综合评价. 草地学报, 2026 , 34 (5) : 1664 -1676 . DOI: 10.11733/j.issn.1007-0435.2026.05.010
    SU Li-he, HUANG Rong-zheng, HU Tian-yu, MA Chun-hui. Comprehensive Evaluation of Salt Tolerance of different Alfalfa (Medicago sativa L.) Cultivars during Germination and Seedling Stages under Salt Stress[J]. Acta Agrestia Sinica, 2026 , 34 (5) : 1664 -1676 . DOI: 10.11733/j.issn.1007-0435.2026.05.010

      国家牧草产业技术体系 (CARS-34)

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    SU L H, ZHANG F F, WANG X Z, et al. Effects of snow cover on physiological characteristics and cold resistance related gene expression of alfalfa varieties with different fall dormancy levels[J]. Chinese Journal of Grassland, 2021, 43(5):18-26 苏力合, 张凡凡, 王旭哲, 等. 覆雪对不同秋眠型紫花苜蓿生理特性及抗寒基因表达的影响[J]. 中国草地学报, 2021, 43(5):18-26
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    E J F, YANG S Q, ZHANG W F, et al. Effect of forage planting on improvement of saline soil under subsurface pipe in agro-pastoral intertwined zones[J]. Transactions of the Chinese Society for Agricultural Machinery, 2024, 55(9):402-410 鄂继芳, 杨树青, 张万锋, 等. 暗管条件下牧草种植对农牧交错区盐渍土的改土效应[J]. 农业机械学报, 2024, 55(9):402-410
    LV Y Q, HU J, GAO D X, et al. Effects of forage cultivation and cattle manure application on nutrient and humus composition of saline alkali soil in the Songnen Plain[J]. Journal of Agricultural Resources and Environment, 2025, 42(1):169-176 吕悦齐, 胡娟, 高东雪, 等. 饲草种植及牛粪施用对松嫩平原盐碱土养分及腐殖质组成的影响[J]. 农业资源与环境学报, 2025, 42(1):169-176
    SHI J Q, XIE N, CUI S Q, et al. Evaluation of salt tolerance in 16 alfalfa varieties during germination under different salt concentration stress[J]. Acta Agrestia Sinica, 2025, 33(2):472-480 石嘉琦, 谢楠, 崔素倩, 等. 不同盐浓度胁迫下16个紫花苜蓿品种萌发期耐盐性评价[J]. 草地学报, 2025, 33(2):472-480
    ZHU K, LIU H J, FENG C L, et al. Comprehensive evaluation on the salt tolerance of seed germination of different alfalfa varieties under salt stress[J]. Acta Agrestia Sinica, 2023, 31(12):3724-3733 朱琨, 刘骅峻, 冯成龙, 等. 盐胁迫对不同苜蓿品种种子萌发的耐盐性综合评价[J]. 草地学报, 2023, 31(12):3724-3733
    WU M M, XU J Y, NIE Z J, et al. Physiological, biochemical and transcriptomic insights into the mechanisms by which molybdenum mitigates cadmium toxicity in Triticum aestivum L[J]. Journal of Hazardous Materials, 2024, 472:134516
    ZHANG X Y, LI S H, TANG T, et al. Comparison of morphological, physiological, and related-gene expression responses to saline-alkali stress in eight apple rootstock genotypes[J]. Scientia Horticulturae, 2022, 306:111455
    WANG M, DING Y, WANG Q, et al. NaCl treatment on physio-biochemical metabolism and phenolics accumulation in barley seedlings[J]. Food chemistry, 2020, 331:127282
    LI H, LIU L, HUANG Q, et al. Identification and screening of salt tolerance of cotton germplasm resources at germination stage[J]. Acta Agronomica Sinica, 2024, 50(5):1147-1157 李航, 刘丽, 黄乾, 等. 棉花种质资源萌发期耐盐性鉴定及筛选[J]. 作物学报, 2024, 50(5):1147-1157
    WANG Y L, WEI L, LI N, et al. Comprehensive evaluation of salt tolerance of three forage seed varieties during germination[J]. Pratacultural Science, 2023, 40(12):3104-3113 王娅琳, 魏琳, 李娜, 等. 3种牧草种子萌发期耐盐性综合评价[J]. 草业科学, 2023, 40(12):3104-3113
    YANG X J, SUN Q Y, ZHANG Z, et al. Comprehensive evaluation on salt tolerance of 61 alfalfa cultivars during seed germination[J]. Acta Agrestia Sinica, 2025, 33(4):1181-1191 姚兴洁, 孙庆赢, 张昭, 等. 61个品种紫花苜蓿种子萌发期耐盐性综合评价[J]. 草地学报, 2025, 33(4):1181-1191
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    XIONG X, GUI W Y, LIU M H, et al. Evaluation of salt tolerance in different alfalfa varieties under uniform and non-uniform salt stress[J]. Acta Prataculturae Sinica, 2018, 27(9):67-76 熊雪, 桂维阳, 刘沫含, 等. 不同紫花苜蓿品种在均匀与不均匀盐胁迫下的耐盐性评价[J]. 草业学报, 2018, 27(9):67-76
    LÜ Y Z, Y X J, ZHANG Z, et al. Evaluation of alkali tolerance and key indicators screening during seed germination of different alfalfa cultivars[J]. Acta Agrestia Sinica, 2025, 33(6):1852-1861 吕艳贞, 姚兴洁, 张昭, 等. 不同品种紫花苜蓿种子萌发期耐碱性评价与关键指标筛选[J]. 草地学报, 2025, 33(6):1852-1861
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    doi: 10.11733/j.issn.1007-0435.2026.05.010
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    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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