草地学报

碱性盐胁迫对白羊草胚根和胚芽线粒体AsA-GSH循环的影响

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草地学报 | 研究论文 2026,34(5): 1798-1806

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草地学报 |研究论文 2026 , 34 (5) : 1798 -1806

碱性盐胁迫对白羊草胚根和胚芽线粒体AsA-GSH循环的影响

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董秋丽（1985-），女，实验师，硕士，主要从事草种科学研究工作，E-mail:sxaudql@163.com

Effects of Alkaline Salt Stress on AsA-GSH Cycle of Mitochondria in the Radicle and Plumule of Bothriochloa ischaemum

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doi: 10.11733/j.issn.1007-0435.2026.05.021

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为探究碱性盐胁迫对白羊草（Bothriochloa ischaemum）胚根和胚芽线粒体抗坏血酸-谷胱甘肽（AsA-GSH）循环的影响，本试验以‘太行’白羊草为材料，在Na⁺浓度为0（CK），10和30 mmol·L⁻¹的NaHCO₃和Na₂CO₃溶液中进行萌发试验，分析其胚根和胚芽线粒体AsA-GSH循环酶活性及脂质过氧化变化规律。结果表明：白羊草胚根线粒体中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和单脱氢抗坏血酸还原酶活性随Na⁺浓度（0~30 mmol·L⁻¹）的增加均呈上升趋势，而其谷胱甘肽硫转移酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶活性则相反；白羊草胚芽线粒体中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性随Na⁺浓度（0~30 mmol·L⁻¹）的增加均显著上升（P<0.05），而其谷胱甘肽硫转移酶、谷胱甘肽还原酶和单脱氢抗坏血酸还原酶活性则显著下降（P<0.05）。碱性盐种类和浓度对白羊草胚根和胚芽AsA-GSH循环的影响存在差异，且胚根线粒体AsA-GSH循环响应碱性盐胁迫的敏感性要高于胚芽。

关键词

碱性盐胁迫 / 白羊草 / 线粒体 / 抗坏血酸-谷胱甘肽循环 / 胚根 / 胚芽

Abstract

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In order to explore the effects of alkaline salt stress on the ascorbic acid-glutathione cycle（AsA-GSH） of mitochondria in the radicle and plumule of Bothriochloa ischaemum， this experiment used ‘Taihang’ Bothriochloa ischaemum as material， which was germinated in NaHCO₃ and Na₂CO₃ solutions with Na⁺ concentration of 0（CK）， 10 and 30 mmol·L^-1， and then changes in the activities of AsA-GSH enzymes and lipid peroxidation in their mitochondria of radicle and plumule were analyzed. The results showed that the activities of superoxide dismutase， catalase， glutathione peroxidase and monodehydroascorbate reductase in the mitochondria of radicle increased with the increase of Na⁺ concentration （0-30 mmol·L^-1）， but the opposite phenomenon was observed in the activities of glutathione sulfur transferase， ascorbate peroxidase and glutathione reductase. With the increase of Na⁺ concentration （0-30 mmol·L^-1）， the activities of superoxide dismutase， catalase and glutathione peroxidase in the mitochondria of plumule increased significantly （P<0.05）， but the activities of glutathione sulfur transferase， glutathione reductase and monodehydroascorbate reductase decreased significantly （P<0.05）. There were differences in the effects of type and concentration under alkaline salt on the AsA-GSH in radicle and plumule of Bothriochloa ischaemum， and the sensitivity of mitochondrial AsA-GSH in radicle of Bothriochloa ischaemum to alkaline salt stress was higher than that in plumule.

Key words

Alkaline salt stress / Bothriochloa ischaemum / Mitochondria / Ascorbic acid-glutathione cycle / Radicle / Plumule

引用本文

董秋丽, 赵晨洋, 柴玲, 陈奕霖, 李尹琳, 夏方山. 碱性盐胁迫对白羊草胚根和胚芽线粒体AsA-GSH循环的影响. 草地学报, 2026 , 34 (5) : 1798 -1806 . DOI: 10.11733/j.issn.1007-0435.2026.05.021

DONG Qiu-li, ZHAO Chen-yang, CHAI Ling, CHEN Yi-lin, LI Yin-lin, XIA Fang-shan. Effects of Alkaline Salt Stress on AsA-GSH Cycle of Mitochondria in the Radicle and Plumule of Bothriochloa ischaemum[J]. Acta Agrestia Sinica, 2026 , 34 (5) : 1798 -1806 . DOI: 10.11733/j.issn.1007-0435.2026.05.021

基金

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山西农业大学“十四五”生物育种工程项目“乡土草种质资源挖掘与新品种选育 (YZGC134)

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2026年第34卷第5期

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doi: 10.11733/j.issn.1007-0435.2026.05.021

接收时间：2025-12-25
首发时间：2026-09-23

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收稿日期：2025-12-25
修回日期：2026-01-28

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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