Article(id=1309573270836441664, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.04.034, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1745424000000, receivedDateStr=2025-04-24, revisedDate=1758297600000, revisedDateStr=2025-09-20, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156198461, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156198461, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156198461, creator=13701087609, updateTime=1790156198461, updator=13701087609, issue=Issue{id=1309573247344144835, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='4', pageStart='1137', pageEnd='1556', issueExtLink='null', onlineDate='null', pubDate='1776182400000', pubDateStr='2026-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156192860, creator='13701087609', updateTime=1790156800047, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575794117144683, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575794117144684, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1534, endPage=1545, ext={EN=ArticleExt(id=1309573271121654338, articleId=1309573270836441664, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Cerium Oxide Nanoparticle Priming on Seed Germination and Physiological Characteristics of Alfalfa under Drought Stress, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Drought stress inhibits seed germination of alfalfa (Medicago sativa L.) and reduces its yield. This research studied the effects of cerium oxide nanoparticle priming on seed germination and physiological characteristics of alfalfa under drought stress. Alfalfa seeds were used as materials and primed with different concentrations of cerium oxide nanoparticle (CeO2NPs) solutions (0, 50, 100, 150, 200 mg·L-1) in the dark for 24 hours. Drought stress was simulated with 15% PEG to explore the mitigating effect of CeO2NPs on seed germination. The results showed that 15% PEG significantly inhibited seed germination, while priming with 150 mg·L-1 CeO2NPs increased the germination rate to 77% (P<0.05), which was 5% higher than the non-primed treatment. It also shortened the average germination time, increased the germination index, and enhanced the fresh weight of germinated seedlings. At the physiological level, the 150 mg·L-1 CeO2NPs priming treatment increased the activities of α-amylase and β-amylase, and significantly reduced the contents of reducing sugar (P<0.05); the 100 mg·L-1 treatment significantly improved the activity of antioxidant enzymes; the 50 mg·L-1 treatment effectively reduced the ROS content; all concentrations of CeO2NPs priming treatments significantly decreased the malondialdehyde content (P<0.05) and the proline content, and increased the soluble protein content as osmotic adjustment substances. Comprehensive analysis indicated that 150 mg·L-1 CeO2NPs priming had the best mitigating effect on alfalfa seed germination under drought stress., authors=GE Meng-ting1, QIAO Dan-dan1, WANG Xin-yue1, ZHANG Ruo-lin1, BI Mingyue1, HE Xue-qing1, authorsList=GE Meng-ting, QIAO Dan-dan, WANG Xin-yue, ZHANG Ruo-lin, BI Mingyue, HE Xue-qing, authorCompany=1 College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573271050351169, articleId=1309573270836441664, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发及生理特性的影响, columnId=1309565376569114713, journalTitle=草地学报, columnName=技术研发, runingTitle=null, highlight=null, articleAbstract=干旱胁迫会抑制紫花苜蓿(Medicago sativa L.)种子萌发,降低产量。为探究纳米氧化铈(CeO₂NPs)引发对干旱胁迫下紫花苜蓿种子萌发的缓解效应,本研究以紫花苜蓿种子为试验材料,采用浓度分别为0,50,100,150,200 mg·L⁻¹的纳米氧化铈悬浮液在黑暗条件下引发处理24 h,通过15% PEG模拟干旱胁迫,进行种子萌发试验。研究结果显示:15% PEG显著抑制种子萌发,而150 mg·L⁻¹纳米氧化铈引发可将发芽率提升至77%(P<0.05),较未引发处理提高5%,同时缩短平均发芽时间、提高发芽指数并增加幼苗鲜重。生理层面,150 mg·L⁻¹纳米氧化铈引发处理提高了α-淀粉酶和β-淀粉酶活性,显著降低还原糖含量(P<0.05);100 mg·L⁻¹处理显著提升抗氧化酶活性,50 mg·L⁻¹处理有效降低ROS含量;各浓度处理均显著降低丙二醛含量(P<0.05),并降低脯氨酸和提高可溶性蛋白等渗透调节物质含量。综合分析表明,150 mg·L⁻¹纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发的缓解效果最佳。, authors=戈孟婷1, 乔丹丹1, 王新月1, 张若琳1, 毕明月1, 何学青1, authorsList=戈孟婷, 乔丹丹, 王新月, 张若琳, 毕明月, 何学青, authorCompany=1 西北农林科技大学草业与草原学院, 陕西 杨凌 712100, correspAuthors=null, authorNote=戈孟婷(1999-),女,汉族,河南南阳人,硕士研究生,主要从事牧草种质资源与逆境生物学研究,E-mail:2022051684@nwafu.edu.cn, correspAuthorsNote=null, copyrightStatement=null, 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SUN Y F. Effects of nanopriming with iron oxide(Ⅲ) on seed germination and drought resistance of switchgrass(Panicum virgatum)[D]. Yangling:Northwest A & F University,2021:47-48 孙运府. 纳米铁引发对柳枝稷种子萌发特性及抗旱性的影响[D]. 杨凌:西北农林科技大学,2021:47-48
SHELAR A,SINGH A V,MAHARJAN R S,et al. Sustainable agriculture through multidisciplinary seed nanopriming: prospects of opportunities and challenges[J]. Cells,2021,10(9):2428
GOHARI G,ZAREEI E,ROSTAMI H,et al. Protective effects of cerium oxide nanoparticles in grapevine (Vitis vinifera L.) cv. flame seedless under salt stress conditions[J]. Ecotoxicology and Environmental Safety,2021,220:112402
KHAN M N,LI Y H,KHAN Z,et al. Nanoceria seed priming enhanced salt tolerance in rapeseed through modulating ROS homeostasis and α-amylase activities[J]. Journal of Nanobiotechnology,2021,19(1):276
ZHAO H M,YOU Y L,LI Y,et al. Study on evaluation method of drought resistance of alfalfa resources[J]. Acta Agrestia Sinica,2017,25(6):1308-1316 赵海明,游永亮,李源,等. 紫花苜蓿资源抗旱性鉴定评价方法研究[J]. 草地学报,2017,25(6):1308-1316
ERICE G,LOUAHLIA S,IRIGOYEN J J,et al. Biomass partitioning,morphology and water status of four alfalfa genotypes submitted to progressive drought and subsequent recovery[J]. Journal of Plant Physiology,2010,167(2):114-120
LI D D. Effects of drought stress on physiology and biochemistry and content of Medicago sativa[D]. Yangling:Northwest A & F University,2020:49-50 李丹丹. 干旱胁迫对紫花苜蓿生理生化和黄酮类化合物含量的影响[D]. 杨凌:西北农林科技大学,2020:49-50
HOSSEINI BOLDAJI S A,KHAVARI-NEJAD R A,HASSAN SAJEDI R,et al. Water availability effects on antioxidant enzyme activities,lipid peroxidation,and reducing sugar contents of alfalfa (Medicago sativa L.)[J]. Acta Physiologiae Plantarum,2012,34(3):1177-1186
CAI H,LI C X,CONG C L,et al. Effect of overexpression of MsRCI2A/B/C genes on drought tolerance of alfalfa (Medicago sativa L.)[J]. Journal of Northwest A & F University (Natural Science Edition),2022,50(2):57-66,74 才华,李淳馨,丛春龙,等. MsRCI2A/B/C基因超量表达对紫花苜蓿耐旱性的影响[J]. 西北农林科技大学学报(自然科学版),2022,50(2):57-66,74
ARSHAD M,FEYISSA B A,AMYOT L,et al. microRNA156 improves drought stress tolerance in alfalfa (Medicago sativa) by silencing SPL13[J]. Plant Science,2017,258:122-136
SONG K X. Nanopriming with green synthesis of silver effects on the germination and physiological characteristics of alfalfa (Medicago sativa L.)[D]. Yangling:Northwest A & F University,2022:25-26 宋克晓. 绿色合成纳米银引发对紫花苜蓿种子萌发及幼苗生理特性影响研究[D]. 杨凌:西北农林科技大学,2022:25-26
GAO J Z. The effect mechanism of rare earth nanopriming on seed germination andseedling growth of alfalfa under salt stress[D]. Yangling:Northwest A & F University,2023:23-24 高金柱. 稀土纳米引发对紫花苜蓿盐胁迫下种子萌发及幼苗生长的影响机制[D]. 杨凌:西北农林科技大学,2023:23-24
GAO J F. Experimental guidance for plant physiology[M]. Beijing: Higher Education Press, 2006: 144-148 高俊凤.植物生理学实验指导[M]. 北京: 高等教育出版社,2006:144-148
ZHANG P,HU Y,ZHOU R J,et al. The antioxidant system response to drought-stressed Diospyros lotus treated with exogenous melatonin[J]. PeerJ,2022,10:e13936
CHEN J. Effects of exogenous melatonin on growth and physiological characteristics of alfalfa under drought stress[D]. Nanjing:Nanjing Agricultural University,2019:10-11 陈洁. 外源褪黑素对干旱胁迫下紫花苜蓿生长和生理性状的影响[D]. 南京:南京农业大学,2019:10-11
LI C D. Evaluation of density-tolerance in soybean germplasm resources and screening of its identification indexes[J]. Soybean Science,2020,39(5):688-695 李灿东. 大豆种质资源耐密性评价及鉴定指标筛选[J]. 大豆科学,2020,39(5):688-695
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LIU W,CHANG Z,HUANG Y. Germination characteristics of Trifolium repens seeds under different stresses[J]. Journal of Wenshan University,2019,32(6):14-18 刘伟,常征,黄勇. 不同胁迫条件下白花三叶草种子的萌发特性[J]. 文山学院学报,2019,32(6):14-18
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FAROOQ M,AZIZ T,REHMAN H UR,et al. Evaluating surface drying and re-drying for wheat seed priming with polyamines: effects on emergence,early seedling growth and starch metabolism[J]. Acta Physiologiae Plantarum,2011,33(5):1707-1713
TIAN Y,GUAN B,ZHOU D W,et al. Responses of seed germination,seedling growth,and seed yield traits to seed pretreatment in maize (Zea mays L.)[J]. The Scientific World Journal,2014,2014(1):834630
ZENG W H,CHENG B Z,PENG Y,et al. Effects on germination and seedling drought tolerance in white clover of seed soaking with mannose[J]. Acta Prataculturae Sinica,2019,28(7):112-122 曾伟航,程碧真,彭燕,等. 甘露糖浸种对干旱胁迫下白三叶种子萌发及抗旱性的影响[J]. 草业学报,2019,28(7):112-122
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ZHONG T W. Effects of carbon nanomaterials on growth of wheat seedlings under drought stress [D]. Qinhuangdao:Hebei Normal University of Science & Technology,2023:45-46 钟婷玮. 干旱胁迫下碳纳米材料对小麦幼苗生长的影响[D]. 秦皇岛:河北科技师范学院,2023:45-46
SI A J,SUN Z W,LI Z K,et al. A genome wide association study revealed key single nucleotide polymorphisms/genes associated with seed germination in Gossypium hirsutum L[J]. Frontiers in Plant Science,2022,13:844946
BAFOIL M,LE RU A,MERBAHI N,et al. New insights of low-temperature plasma effects on germination of three genotypes of Arabidopsis thaliana seeds under osmotic and saline stresses[J]. Scientific Reports,2019,9:8649
GU Z C,HU C J,GAN Y X,et al. Role of microbes in alleviating crop drought stress: a review[J]. Plants,2024,13(3):384
DELLA NOCE B,MARTINS DA SILVA R,DE CARVALHO UHL M V,et al. Redox imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line[J]. The Journal of Biological Chemistry,2022,298(3):101599)
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纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发及生理特性的影响
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草地学报 | 技术研发 2026,34(4): 1534-1545
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草地学报 |技术研发 2026 , 34 (4) : 1534 -1545
纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发及生理特性的影响
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作者信息
作者简介:
戈孟婷(1999-),女,汉族,河南南阳人,硕士研究生,主要从事牧草种质资源与逆境生物学研究,E-mail:2022051684@nwafu.edu.cn
Effects of Cerium Oxide Nanoparticle Priming on Seed Germination and Physiological Characteristics of Alfalfa under Drought Stress
Affiliations
    1 College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China
doi: 10.11733/j.issn.1007-0435.2026.04.034
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干旱胁迫会抑制紫花苜蓿(Medicago sativa L.)种子萌发,降低产量。为探究纳米氧化铈(CeO₂NPs)引发对干旱胁迫下紫花苜蓿种子萌发的缓解效应,本研究以紫花苜蓿种子为试验材料,采用浓度分别为0,50,100,150,200 mg·L⁻¹的纳米氧化铈悬浮液在黑暗条件下引发处理24 h,通过15% PEG模拟干旱胁迫,进行种子萌发试验。研究结果显示:15% PEG显著抑制种子萌发,而150 mg·L⁻¹纳米氧化铈引发可将发芽率提升至77%(P<0.05),较未引发处理提高5%,同时缩短平均发芽时间、提高发芽指数并增加幼苗鲜重。生理层面,150 mg·L⁻¹纳米氧化铈引发处理提高了α-淀粉酶和β-淀粉酶活性,显著降低还原糖含量(P<0.05);100 mg·L⁻¹处理显著提升抗氧化酶活性,50 mg·L⁻¹处理有效降低ROS含量;各浓度处理均显著降低丙二醛含量(P<0.05),并降低脯氨酸和提高可溶性蛋白等渗透调节物质含量。综合分析表明,150 mg·L⁻¹纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发的缓解效果最佳。
紫花苜蓿  /  干旱胁迫  /  纳米氧化铈  /  种子引发  /  种子萌发
Drought stress inhibits seed germination of alfalfa (Medicago sativa L.) and reduces its yield. This research studied the effects of cerium oxide nanoparticle priming on seed germination and physiological characteristics of alfalfa under drought stress. Alfalfa seeds were used as materials and primed with different concentrations of cerium oxide nanoparticle (CeO2NPs) solutions (0, 50, 100, 150, 200 mg·L-1) in the dark for 24 hours. Drought stress was simulated with 15% PEG to explore the mitigating effect of CeO2NPs on seed germination. The results showed that 15% PEG significantly inhibited seed germination, while priming with 150 mg·L-1 CeO2NPs increased the germination rate to 77% (P<0.05), which was 5% higher than the non-primed treatment. It also shortened the average germination time, increased the germination index, and enhanced the fresh weight of germinated seedlings. At the physiological level, the 150 mg·L-1 CeO2NPs priming treatment increased the activities of α-amylase and β-amylase, and significantly reduced the contents of reducing sugar (P<0.05); the 100 mg·L-1 treatment significantly improved the activity of antioxidant enzymes; the 50 mg·L-1 treatment effectively reduced the ROS content; all concentrations of CeO2NPs priming treatments significantly decreased the malondialdehyde content (P<0.05) and the proline content, and increased the soluble protein content as osmotic adjustment substances. Comprehensive analysis indicated that 150 mg·L-1 CeO2NPs priming had the best mitigating effect on alfalfa seed germination under drought stress.
Alfalfa  /  Drought stress  /  Cerium Oxide Nanoparticles  /  Seed priming  /  Seed germination
戈孟婷, 乔丹丹, 王新月, 张若琳, 毕明月, 何学青. 纳米氧化铈引发对干旱胁迫下紫花苜蓿种子萌发及生理特性的影响. 草地学报, 2026 , 34 (4) : 1534 -1545 . DOI: 10.11733/j.issn.1007-0435.2026.04.034
GE Meng-ting, QIAO Dan-dan, WANG Xin-yue, ZHANG Ruo-lin, BI Mingyue, HE Xue-qing. Effects of Cerium Oxide Nanoparticle Priming on Seed Germination and Physiological Characteristics of Alfalfa under Drought Stress[J]. Acta Agrestia Sinica, 2026 , 34 (4) : 1534 -1545 . DOI: 10.11733/j.issn.1007-0435.2026.04.034

    国家重点研发计划项目 (2022YFD1300803); 大学生科技创新项目 (202310712050)

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KHAN M N,LI Y H,KHAN Z,et al. Nanoceria seed priming enhanced salt tolerance in rapeseed through modulating ROS homeostasis and α-amylase activities[J]. Journal of Nanobiotechnology,2021,19(1):276
ZHAO H M,YOU Y L,LI Y,et al. Study on evaluation method of drought resistance of alfalfa resources[J]. Acta Agrestia Sinica,2017,25(6):1308-1316 赵海明,游永亮,李源,等. 紫花苜蓿资源抗旱性鉴定评价方法研究[J]. 草地学报,2017,25(6):1308-1316
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CAI H,LI C X,CONG C L,et al. Effect of overexpression of MsRCI2A/B/C genes on drought tolerance of alfalfa (Medicago sativa L.)[J]. Journal of Northwest A & F University (Natural Science Edition),2022,50(2):57-66,74 才华,李淳馨,丛春龙,等. MsRCI2A/B/C基因超量表达对紫花苜蓿耐旱性的影响[J]. 西北农林科技大学学报(自然科学版),2022,50(2):57-66,74
ARSHAD M,FEYISSA B A,AMYOT L,et al. microRNA156 improves drought stress tolerance in alfalfa (Medicago sativa) by silencing SPL13[J]. Plant Science,2017,258:122-136
SONG K X. Nanopriming with green synthesis of silver effects on the germination and physiological characteristics of alfalfa (Medicago sativa L.)[D]. Yangling:Northwest A & F University,2022:25-26 宋克晓. 绿色合成纳米银引发对紫花苜蓿种子萌发及幼苗生理特性影响研究[D]. 杨凌:西北农林科技大学,2022:25-26
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ZHANG P,HU Y,ZHOU R J,et al. The antioxidant system response to drought-stressed Diospyros lotus treated with exogenous melatonin[J]. PeerJ,2022,10:e13936
CHEN J. Effects of exogenous melatonin on growth and physiological characteristics of alfalfa under drought stress[D]. Nanjing:Nanjing Agricultural University,2019:10-11 陈洁. 外源褪黑素对干旱胁迫下紫花苜蓿生长和生理性状的影响[D]. 南京:南京农业大学,2019:10-11
LI C D. Evaluation of density-tolerance in soybean germplasm resources and screening of its identification indexes[J]. Soybean Science,2020,39(5):688-695 李灿东. 大豆种质资源耐密性评价及鉴定指标筛选[J]. 大豆科学,2020,39(5):688-695
WANG J Y,XU W N,SU Y,et al. Characterization and comparison of drought resistance of five alfalfa varieties during germination period under PEG-6000 simulated drought stress[J]. Animal Husbandry and Feed Science,2023,44(2):81-91 王江银,徐婉宁,苏洋,等. PEG-6000模拟干旱胁迫下5份苜蓿材料萌发期抗旱性鉴定与比较[J]. 畜牧与饲料科学,2023,44(2):81-91
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LIZZI D,MATTIELLO A,PIANI B,et al. Germination and early development of three spontaneous plant species exposed to nanoceria (nCeO2) with different concentrations and particle sizes[J]. Nanomaterials,2020,10(12):2534
FAROOQ M,AZIZ T,REHMAN H UR,et al. Evaluating surface drying and re-drying for wheat seed priming with polyamines: effects on emergence,early seedling growth and starch metabolism[J]. Acta Physiologiae Plantarum,2011,33(5):1707-1713
TIAN Y,GUAN B,ZHOU D W,et al. Responses of seed germination,seedling growth,and seed yield traits to seed pretreatment in maize (Zea mays L.)[J]. The Scientific World Journal,2014,2014(1):834630
ZENG W H,CHENG B Z,PENG Y,et al. Effects on germination and seedling drought tolerance in white clover of seed soaking with mannose[J]. Acta Prataculturae Sinica,2019,28(7):112-122 曾伟航,程碧真,彭燕,等. 甘露糖浸种对干旱胁迫下白三叶种子萌发及抗旱性的影响[J]. 草业学报,2019,28(7):112-122
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ZHONG T W. Effects of carbon nanomaterials on growth of wheat seedlings under drought stress [D]. Qinhuangdao:Hebei Normal University of Science & Technology,2023:45-46 钟婷玮. 干旱胁迫下碳纳米材料对小麦幼苗生长的影响[D]. 秦皇岛:河北科技师范学院,2023:45-46
SI A J,SUN Z W,LI Z K,et al. A genome wide association study revealed key single nucleotide polymorphisms/genes associated with seed germination in Gossypium hirsutum L[J]. Frontiers in Plant Science,2022,13:844946
BAFOIL M,LE RU A,MERBAHI N,et al. New insights of low-temperature plasma effects on germination of three genotypes of Arabidopsis thaliana seeds under osmotic and saline stresses[J]. Scientific Reports,2019,9:8649
GU Z C,HU C J,GAN Y X,et al. Role of microbes in alleviating crop drought stress: a review[J]. Plants,2024,13(3):384
DELLA NOCE B,MARTINS DA SILVA R,DE CARVALHO UHL M V,et al. Redox imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line[J]. The Journal of Biological Chemistry,2022,298(3):101599
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doi: 10.11733/j.issn.1007-0435.2026.04.034
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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