Article(id=1309573270333128822, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.04.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1748880000000, receivedDateStr=2025-06-03, revisedDate=1752595200000, revisedDateStr=2025-07-16, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156198341, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156198341, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156198341, creator=13701087609, updateTime=1790156198341, updator=13701087609, issue=Issue{id=1309573247344144835, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='4', pageStart='1137', pageEnd='1556', issueExtLink='null', onlineDate='null', pubDate='1776182400000', pubDateStr='2026-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156192860, creator='13701087609', updateTime=1790156800047, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575794117144683, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575794117144684, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1196, endPage=1204, ext={EN=ArticleExt(id=1309573273151701116, articleId=1309573270333128822, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Isolation, Identification, and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To screen biocontrol strains with high herbicidal potential, the pathogenic fungus JZ-28 was isolated from naturally infected leaves of Viola philippica in Qinghai Province. Through morphological characterization and multi-gene (ITS, EF-1α, LSU) phylogenetic analysis, the isolate was identified as Clonostachys rosea. Pathogenicity assays demonstrated potent herbicidal activity of strain JZ-28, inducing chlorosis in detached leaves of four dominant broadleaf weed species from the Qinghai-Xizang Plateau(Malva verticillata, Elsholtzia densa, Senecio vulgaris, and Chenopodium album)within 7 days post-inoculation (dpi). Disease incidence rates in whole-plant assays reached 83%, 95%, 100%, and 70%, respectively. Safety evaluation on six major crops and three cool-season vegetables in Qinghai revealed that JZ-28 was safe for four crops: Zea mays, Vicia faba, Pisum sativum, Brassica napus, and three vegetables: Cucumis sativus, Solanum lycopersicum, and Solanum melongena. Lightly Susceptible was observed in highland barley Hordeum vulgare and Triticum aestivum. These findings indicated JZ-28’s potential as a broad-spectrum bioherbicide against broadleaf weeds in multiple cropping systems. Culture optimization identified sucrose and soybean meal as optimal carbon and nitrogen sources for enhancing mycelial growth and sporulation. This study provided the first evidence of Clonostachys rosea’s bioherbicide potential in alpine ecosystems, addressing a critical research gap in microbial herbicide development and offering sustainable weed management solutions for fragile environments., authors=PENG Chao1,2, YANG Lan1,2, CUI Wen-jing1,2, MA Yong-qiang1,2,3, CHENG Liang1,2,3, ZHU Hai-xia1,2,3, authorsList=PENG Chao, YANG Lan, CUI Wen-jing, MA Yong-qiang, CHENG Liang, ZHU Hai-xia, authorCompany=1 Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai Province 810016, China;
2 Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Xining, Qinghai Province 810016, China;
3 Scientific Observation and Experiment Station of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai Province 810016, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573271151018105, articleId=1309573270333128822, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为筛选具有高效除草潜力的生防菌株,本研究从青海省自然感病紫花地丁(Viola philippica)叶片中分离出致病菌JZ-28。通过形态学及多基因 (ITS/EF-1α/LSU) 联合鉴定,确定为粉红螺旋聚孢霉(Clonostachys rosea)。致病性试验表明其具有强效除草活性,JZ-28在7 d可使青藏高原4种优势阔叶类杂草冬葵(Malva verticillata)、密花香薷(Elsholtzia densa)、欧洲千里光(Senecio vulgaris)和藜(Chenopodium album)的离体叶片黄化发病,活体植株试验发病率分别为83%,95%,100%,70%。评价JZ-28对青海省6种主栽作物和3种冷凉蔬菜的安全性,表明该菌株对玉米(Zea mays)、蚕豆(Vicia faba)、豌豆(Pisum sativum)、油菜(Brassica napus)4种作物和黄瓜(Cucumis sativus)、番茄(Solanum lycopersicum)、茄子(Solanum melongena)3种冷凉蔬菜表现为安全,对青稞(Hordeum vulgare)、小麦(Triticum aestivum)表现为轻微感病。培养条件优化试验表明,蔗糖与大豆粉分别为最佳碳、氮源,可促进菌丝生长与孢子产量提高。本研究首次揭示了粉红螺旋聚孢霉作为高寒地区生物除草剂的潜力,为脆弱生态系统的杂草可持续管理提供了新的途径。, authors=彭超1,2, 杨兰1,2, 崔文静1,2, 马永强1,2,3, 程亮1,2,3, 朱海霞1,2,3, authorsList=彭超, 杨兰, 崔文静, 马永强, 程亮, 朱海霞, authorCompany=1 青海大学农林科学院, 青海 西宁 810016;
2 青海省农业有害生物综合治理重点实验室, 青海 西宁 810016;
3 农业农村部西宁作物有害生物科学观测实验站, 青海 西宁 810016, correspAuthors=null, authorNote=彭超(2001-),男,汉族,江西吉安人,硕士研究生,主要从事生物防治研究,E-mail:pengchao0201@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=qxJer7YF03SWbaTyqMHMYg==, pdfFileSize=11561765, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目 (32460683); 青海省科技厅基础研究项目 (2024-ZJ-928))}, authors=null, keywords=[Keyword(id=1309573273634046077, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=CN, orderNo=1, keyword=杂草防控), Keyword(id=1309573273705349246, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=CN, orderNo=2, keyword=粉红螺旋聚孢霉), Keyword(id=1309573273998950527, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=CN, orderNo=3, keyword=微生物除草剂), Keyword(id=1309573274120585344, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=EN, orderNo=1, keyword=Weed control), Keyword(id=1309573274422575233, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=EN, orderNo=2, keyword=Clonostachys rosea), Keyword(id=1309573274481295490, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573270333128822, language=EN, orderNo=3, keyword=Microbial herbicides)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1308395841968504927, delFlag=0, nameCn=草地学报, nameEn=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, issn=1007-0435, eissn=null, cn=11-3362/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=gCdi9sXCX82eLR6S1d4VLg==, journalPrice=null, startedYear=null, abbrevIsoEn=Acta Agrestia Sinica, journalRemark=null, publicationField=null, createdTime=1789875477545, updatedTime=1789876241473, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=gCdi9sXCX82eLR6S1d4VLg==, picEn=4dKqEDfsaGuyf+bpm/3gfw==, jcr=null, cjcr=null, exts=[JournalExt(id=1308399046192812446, language=CN, name=草地学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241491, updatedTime=1789876241491, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1308399046268309919, language=EN, name=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241509, updatedTime=1789876241509, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1308397196074385509, websiteList=[Website(id=1308429426430071695, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/CN, language=CN, createTime=1789883484704, createBy=18614031015, updateTime=1789883745058, updateBy=18614031015, name=草地学报-中文, tplId=1146099689490845704, title=草地学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430669353340972, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=articleTextType, value=kx, createTime=1789883781040, updateTime=1789883781040, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669328175145, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=banner, value=null, createTime=1789883781034, updateTime=1789883781034, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669382701103, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=grayFlag, value=0, createTime=1789883781047, updateTime=1789883781047, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669319786536, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883781032, updateTime=1789883781032, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669395284017, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=minRunFlag, value=0, createTime=1789883781050, updateTime=1789883781050, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669344952363, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic, createTime=1789883781038, updateTime=1789883781038, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669391089712, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=silenceFlag, value=0, createTime=1789883781049, updateTime=1789883781049, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669336563754, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1789883781036, updateTime=1789883781036, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669361729581, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeColor, value=null, createTime=1789883781042, updateTime=1789883781042, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669370118190, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeStyle, value=null, createTime=1789883781044, updateTime=1789883781044, creator=18614031015, updator=18614031015)]), Website(id=1308429426501374870, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/EN, language=EN, createTime=1789883484721, createBy=18614031015, updateTime=1789883716139, updateBy=18614031015, name=草地学报-英文, tplId=1146101810881728533, title=Acta Agrestia Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430635979264030, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=articleTextType, value=kx, createTime=1789883773083, updateTime=1789883773083, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635954098203, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=banner, value=null, createTime=1789883773077, updateTime=1789883773077, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636000235553, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=grayFlag, value=0, createTime=1789883773088, updateTime=1789883773088, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635945709594, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883773075, updateTime=1789883773075, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636012818467, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=minRunFlag, value=0, createTime=1789883773091, updateTime=1789883773091, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635970875421, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic, createTime=1789883773081, updateTime=1789883773081, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636004429858, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=silenceFlag, value=0, createTime=1789883773089, updateTime=1789883773089, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635962486812, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1789883773079, updateTime=1789883773079, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635987652639, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeColor, value=null, createTime=1789883773085, updateTime=1789883773085, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635991846944, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeStyle, value=null, createTime=1789883773086, updateTime=1789883773086, creator=18614031015, updator=18614031015)])], journalTitle=草地学报, weixinUrl=null, journalUrl=https://manu40.magtech.com.cn/Jweb_cdxb, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Agrestia Sinica, journalPhotoCn=gCdi9sXCX82eLR6S1d4VLg==, journalPhotoEn=4dKqEDfsaGuyf+bpm/3gfw==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.04.006, detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.04.006, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.04.006, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.04.006, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1776182400000, fullTextJson=null, articleText=null, reference=YU J F,WANG J X,CHEN M X,et al. Studies on eco-economic thresholds of mixed-growing weeds in wheat fields[J]. Plant Protection,2002(5):13-15 于金凤,王金信,陈茂学,等. 麦田混生杂草生态经济阈值的研究[J]. 植物保护,2002(5):13-15
JOSE R P,GERRIT S,TONI V,et al. Genetically engineered crops: from idea to product[J]. Annual Review of Plant Biology,2014,65(1):769-790
PAN L,LIU M,LIU W T,et al. Current situation and prospects of weed science in China[J]. Plant Protection,2023,49(5):295-302,389 潘浪,刘敏,刘伟堂,等. 我国杂草科学学科发展现状与展望[J]. 植物保护,2023,49(5):295-302,389
ZHAN J X,LIU Y, ZHANG S X,et al. Screening of herbicidal activities of plant pathogenic fungi and active metabolites of strain CY-H[J]. Acta Microbiologica Sinica,2020,60(3):582-589 张金新,刘颖,张蜀香,等. 植物病原真菌抑制杂草的活性筛选及菌株CY-H的活性代谢产物[J]. 微生物学报,2020,60(3):582-589
QIU D W. Research progress and prospect of bio pesticides[J]. Plant Protection,2013,39(5):81-89 邱德文. 生物农药研究进展与未来展望[J]. 植物保护,2013,39(5):81-89
XIE W,LI L, Research progress on weed control by using plant pathogenic fung[J]. Fujian Agricultural Science and Technology,2024,55(9):1-7 谢文,李力. 利用植物病原真菌除草的研究进展[J]. 福建农业科技,2024,55(9):1-7
ZHU H X ,MA Y Q. Study on fermentation optimization of herbicidal activity strain Alternaria tenuissima[J]. Journal of Gansu Agricultural University,2020,55 (1):63-71 朱海霞,马永强. 除草活性菌株极细链格孢菌(Alternaria tenuissima)发酵工艺研究[J]. 甘肃农业大学学报,2020,55 (1):63-71
XU Z L. Secondary metabolites of Colletotrichum gloeosporioides BWH-1: isolation,identification and biological activities study[D]. Guangzhou: South China Agricultural University,2020:48 徐兆林. Colletotrichum gloeosporioides BWH-1次级代谢产物的分离、鉴定和生物活性研究[D]. 广州:华南农业大学,2020:48
EVIDENTE A,ABOUZEID M A,ANDOLFI A,et al. Recent achievements in the bio-control of Orobanche infesting important crops in the mediterranean basin[J]. Journal of Agricultural Science and Technology,2011,1:461-483
KRUPSKA J,WATSON A K. Curvularia eragrostidis isolates (Dematiaceae) for biocontrol of crabgrass (Digitaria spp.) in Canada[J]. Biocontrol Science and Technology,2021,31(9):924-950
WU H X,YUAN M L,JANG N,et al. Screening and evaluation of highly efficient biocontrol strains of Clonostachys rosea[J]. Plant Protection,2021,47(3):54-60,95 吴海霞,袁梦蕾,江娜,等. 粉红螺旋聚孢霉高效生防菌株的筛选与评价[J]. 植物保护,2021,47(3):54-60,95
ZHANG J,GUO X P,XIA M C,et al. Optimization of solid state fermentation conditions of Clonostachys rosea NF-06 and its control efficiency on Meloidogyne incognita[J]. Chinese Journal of Biological Control,2020,36(1):105-112 张洁,郭雪萍,夏明聪,等. 粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果[J]. 中国生物防治学报,2020,36(1):105-112
TIAN T,SUN M H,LI S D. Management of cucumber Fusarium wilt via Combinative application of Dazomet and Clonostachys rosea 67-1[J]. Chinese Journal of Biological Control,2014,30(4):503-510 田甜,孙漫红,李世东. 棉隆与粉红螺旋聚孢霉67-1协同防治黄瓜枯萎病的研究[J]. 中国生物防治学报,2014,30(4):503-510
FANG Z D. Plant pathology research methods[M]. Beijing:China Agriculture Press,2007:122-140 方中达. 植病研究法[M]. 北京:中国农业出版社,2007:122-140
WEI J C. Manual of fungal identification[M]. Shanghai:Shanghai Scientific and Technical Publishers,1979:141-159 魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社,1979:141-159
JANG N,WANG Y P,XUE H,et al. A new species of Clonostachys from frozen soil in mount Qomolangma, China[J/OL]. https://link.cnki.net/doi/10.13341/j.jfr.2024.1804, 2025-01-03/2025-05-30 姜宁,王永鹏,薛寒,等. 采自珠穆朗玛峰冻土的螺旋聚孢霉属1新种[J/OL]. https://link.cnki.net/doi/10.13341/j.jfr.2024.1804, 2025-01-03/2025-05-30
LI H,ZHU H X. Isolation and identification of Alternaria alternata strain HY-063 and its herbicidal potential[J]. Plant Protection,2022,48(6):289-297,325 李欢,朱海霞. 链格孢菌株HY-063的分离鉴定及除草潜力[J]. 植物保护,2022,48(6):289-297,325
ZHU H X. Study on herbicidal activity and biological characteristics of Epicoccum strain DT-014[J]. Agricultural Research in the Arid Areas,2024,42(1):237-241,251 朱海霞. 附球属真菌DT-014对杂草的致病性及生物学特性研究[J]. 干旱地区农业研究,2024,42(1):237-241,251
REICHERT JÚNIOR F W,SCARIOT M A,FORTE C T,et al. New perspectives for weeds control using autochthonous fungi with selective bioherbicide potential[J]. Heliyon,2019,5(5):e01676
ANDERSEN C B,JØRGENSEN H J L,MANZOTTI A,et al. Seed coating with the fungal biocontrol agent Clonostachys rosea controls clubroot in oilseed rape[R]. International Organisation for Biological Control,2018,136:157-163
BORGES Á V,SARAIVA R M,MAFFIA L A. Maffia. Biocontrol of gray mold in tomato plants by Clonostachys rosea[J]. Tropical Plant Pathology,2015,40(2):71-76
SUN Y M,WU H L,ZHANG T T,et al. The growth promoting effect of 5406 actinomycetes on watermelon and the control of watermelon Fusarium wilt[J]. Science Technology and Engineering,2021,21(15):6242-6248 孙于淼,吴慧玲,张涛涛,等. 5406放线菌对西瓜的促生作用及对西瓜枯萎病的防控[J]. 科学技术与工程,2021,21(15):6242-6248
IQBAL M,DUBEY M,MCEWAN K,et al. Evaluation of Clonostachys rosea for control of plant-parasitic nematodes in soil and in roots of carrot and wheat[J]. Phytopathology,2018,108(1):52-59
ASKEW D J,LAING M D. An adapted selective medium for the quantitative isolation of Trichoderma species[J]. Plant Pathology,1993,42(5):686–690.
CHATTERTON S,PUNJA Z K. Chitinase and beta-1,3-glucanase enzyme production by the mycoparasite Clonostachys rosea f. catenulata against fungal plant pathogens[J]. Canadian Journal of Microbiology,2009,55(4):356-367
GAO Z Y,GAN J E,ZHANG L J,et al. A review and current status of weed control using phytopathogenic microorganisms in China[J]. Chinese Agricultural Science Bulletin,1995,(1):31-34,37 高昭远,干静娥,张立洁,等. 我国利用植物病原微生物防除杂草研究的回顾与现状[J]. 中国农学通报,1995,(1):31-34,37
NIE Y F,CHENG Z Y,LIU Y F,et al. Conidial production of Nimbya alternantherae SF-193 and its pathogenicity to Alternanthera philoxeroides,2009,25(3):260-266 聂亚锋,陈志谊,刘永锋,等. 假隔链格孢SF-193的产孢特性及其分生孢子对空心莲子草的致病力[J]. 中国生物防治,2009,25(3):260-266
OU S S,ZHANG S J. Biopesticides and biofertilizers[M]. Beijing:Chemical Industry Press,2019:31-32 欧善生,张慎举. 生物农药与肥料[M]. 北京:化学出版社,2019:31-32)
收藏切换
杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价
收藏切换
PDF下载
草地学报 | 研究论文 2026,34(4): 1196-1204
收起
收藏切换
草地学报 |研究论文 2026 , 34 (4) : 1196 -1204
杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价
全屏
彭超1,2, 杨兰1,2, 崔文静1,2, 马永强1,2,3, 程亮1,2,3, 朱海霞1,2,3
作者信息
    1 青海大学农林科学院, 青海 西宁 810016;
    2 青海省农业有害生物综合治理重点实验室, 青海 西宁 810016;
    3 农业农村部西宁作物有害生物科学观测实验站, 青海 西宁 810016
作者简介:
彭超(2001-),男,汉族,江西吉安人,硕士研究生,主要从事生物防治研究,E-mail:pengchao0201@163.com
Isolation, Identification, and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28
  • PENG Chao1,2, YANG Lan1,2, CUI Wen-jing1,2, MA Yong-qiang1,2,3, CHENG Liang1,2,3, ZHU Hai-xia1,2,3
  • Affiliations
      1 Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai Province 810016, China;
      2 Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province, Xining, Qinghai Province 810016, China;
      3 Scientific Observation and Experiment Station of Crop Pests, Ministry of Agriculture and Rural Affairs, Xining, Qinghai Province 810016, China
    doi: 10.11733/j.issn.1007-0435.2026.04.006
    文章导航
    收藏切换
    为筛选具有高效除草潜力的生防菌株,本研究从青海省自然感病紫花地丁(Viola philippica)叶片中分离出致病菌JZ-28。通过形态学及多基因 (ITS/EF-1α/LSU) 联合鉴定,确定为粉红螺旋聚孢霉(Clonostachys rosea)。致病性试验表明其具有强效除草活性,JZ-28在7 d可使青藏高原4种优势阔叶类杂草冬葵(Malva verticillata)、密花香薷(Elsholtzia densa)、欧洲千里光(Senecio vulgaris)和藜(Chenopodium album)的离体叶片黄化发病,活体植株试验发病率分别为83%,95%,100%,70%。评价JZ-28对青海省6种主栽作物和3种冷凉蔬菜的安全性,表明该菌株对玉米(Zea mays)、蚕豆(Vicia faba)、豌豆(Pisum sativum)、油菜(Brassica napus)4种作物和黄瓜(Cucumis sativus)、番茄(Solanum lycopersicum)、茄子(Solanum melongena)3种冷凉蔬菜表现为安全,对青稞(Hordeum vulgare)、小麦(Triticum aestivum)表现为轻微感病。培养条件优化试验表明,蔗糖与大豆粉分别为最佳碳、氮源,可促进菌丝生长与孢子产量提高。本研究首次揭示了粉红螺旋聚孢霉作为高寒地区生物除草剂的潜力,为脆弱生态系统的杂草可持续管理提供了新的途径。
    杂草防控  /  粉红螺旋聚孢霉  /  微生物除草剂
    To screen biocontrol strains with high herbicidal potential, the pathogenic fungus JZ-28 was isolated from naturally infected leaves of Viola philippica in Qinghai Province. Through morphological characterization and multi-gene (ITS, EF-1α, LSU) phylogenetic analysis, the isolate was identified as Clonostachys rosea. Pathogenicity assays demonstrated potent herbicidal activity of strain JZ-28, inducing chlorosis in detached leaves of four dominant broadleaf weed species from the Qinghai-Xizang Plateau(Malva verticillata, Elsholtzia densa, Senecio vulgaris, and Chenopodium album)within 7 days post-inoculation (dpi). Disease incidence rates in whole-plant assays reached 83%, 95%, 100%, and 70%, respectively. Safety evaluation on six major crops and three cool-season vegetables in Qinghai revealed that JZ-28 was safe for four crops: Zea mays, Vicia faba, Pisum sativum, Brassica napus, and three vegetables: Cucumis sativus, Solanum lycopersicum, and Solanum melongena. Lightly Susceptible was observed in highland barley Hordeum vulgare and Triticum aestivum. These findings indicated JZ-28’s potential as a broad-spectrum bioherbicide against broadleaf weeds in multiple cropping systems. Culture optimization identified sucrose and soybean meal as optimal carbon and nitrogen sources for enhancing mycelial growth and sporulation. This study provided the first evidence of Clonostachys rosea’s bioherbicide potential in alpine ecosystems, addressing a critical research gap in microbial herbicide development and offering sustainable weed management solutions for fragile environments.
    Weed control  /  Clonostachys rosea  /  Microbial herbicides
    彭超, 杨兰, 崔文静, 马永强, 程亮, 朱海霞. 杂草致病菌株粉红螺旋聚孢霉JZ-28的分离、鉴定及除草潜力评价. 草地学报, 2026 , 34 (4) : 1196 -1204 . DOI: 10.11733/j.issn.1007-0435.2026.04.006
    PENG Chao, YANG Lan, CUI Wen-jing, MA Yong-qiang, CHENG Liang, ZHU Hai-xia. Isolation, Identification, and Herbicidal Potential Evaluation of Pathogenic Weed Strain Clonostachys rosea JZ-28[J]. Acta Agrestia Sinica, 2026 , 34 (4) : 1196 -1204 . DOI: 10.11733/j.issn.1007-0435.2026.04.006

      国家自然科学基金项目 (32460683); 青海省科技厅基础研究项目 (2024-ZJ-928)

    参考文献 引证文献
    排序方式:
    YU J F,WANG J X,CHEN M X,et al. Studies on eco-economic thresholds of mixed-growing weeds in wheat fields[J]. Plant Protection,2002(5):13-15 于金凤,王金信,陈茂学,等. 麦田混生杂草生态经济阈值的研究[J]. 植物保护,2002(5):13-15
    JOSE R P,GERRIT S,TONI V,et al. Genetically engineered crops: from idea to product[J]. Annual Review of Plant Biology,2014,65(1):769-790
    PAN L,LIU M,LIU W T,et al. Current situation and prospects of weed science in China[J]. Plant Protection,2023,49(5):295-302,389 潘浪,刘敏,刘伟堂,等. 我国杂草科学学科发展现状与展望[J]. 植物保护,2023,49(5):295-302,389
    ZHAN J X,LIU Y, ZHANG S X,et al. Screening of herbicidal activities of plant pathogenic fungi and active metabolites of strain CY-H[J]. Acta Microbiologica Sinica,2020,60(3):582-589 张金新,刘颖,张蜀香,等. 植物病原真菌抑制杂草的活性筛选及菌株CY-H的活性代谢产物[J]. 微生物学报,2020,60(3):582-589
    QIU D W. Research progress and prospect of bio pesticides[J]. Plant Protection,2013,39(5):81-89 邱德文. 生物农药研究进展与未来展望[J]. 植物保护,2013,39(5):81-89
    XIE W,LI L, Research progress on weed control by using plant pathogenic fung[J]. Fujian Agricultural Science and Technology,2024,55(9):1-7 谢文,李力. 利用植物病原真菌除草的研究进展[J]. 福建农业科技,2024,55(9):1-7
    ZHU H X ,MA Y Q. Study on fermentation optimization of herbicidal activity strain Alternaria tenuissima[J]. Journal of Gansu Agricultural University,2020,55 (1):63-71 朱海霞,马永强. 除草活性菌株极细链格孢菌(Alternaria tenuissima)发酵工艺研究[J]. 甘肃农业大学学报,2020,55 (1):63-71
    XU Z L. Secondary metabolites of Colletotrichum gloeosporioides BWH-1: isolation,identification and biological activities study[D]. Guangzhou: South China Agricultural University,2020:48 徐兆林. Colletotrichum gloeosporioides BWH-1次级代谢产物的分离、鉴定和生物活性研究[D]. 广州:华南农业大学,2020:48
    EVIDENTE A,ABOUZEID M A,ANDOLFI A,et al. Recent achievements in the bio-control of Orobanche infesting important crops in the mediterranean basin[J]. Journal of Agricultural Science and Technology,2011,1:461-483
    KRUPSKA J,WATSON A K. Curvularia eragrostidis isolates (Dematiaceae) for biocontrol of crabgrass (Digitaria spp.) in Canada[J]. Biocontrol Science and Technology,2021,31(9):924-950
    WU H X,YUAN M L,JANG N,et al. Screening and evaluation of highly efficient biocontrol strains of Clonostachys rosea[J]. Plant Protection,2021,47(3):54-60,95 吴海霞,袁梦蕾,江娜,等. 粉红螺旋聚孢霉高效生防菌株的筛选与评价[J]. 植物保护,2021,47(3):54-60,95
    ZHANG J,GUO X P,XIA M C,et al. Optimization of solid state fermentation conditions of Clonostachys rosea NF-06 and its control efficiency on Meloidogyne incognita[J]. Chinese Journal of Biological Control,2020,36(1):105-112 张洁,郭雪萍,夏明聪,等. 粉红螺旋聚孢霉NF-06固体发酵条件优化及对南方根结线虫的防治效果[J]. 中国生物防治学报,2020,36(1):105-112
    TIAN T,SUN M H,LI S D. Management of cucumber Fusarium wilt via Combinative application of Dazomet and Clonostachys rosea 67-1[J]. Chinese Journal of Biological Control,2014,30(4):503-510 田甜,孙漫红,李世东. 棉隆与粉红螺旋聚孢霉67-1协同防治黄瓜枯萎病的研究[J]. 中国生物防治学报,2014,30(4):503-510
    FANG Z D. Plant pathology research methods[M]. Beijing:China Agriculture Press,2007:122-140 方中达. 植病研究法[M]. 北京:中国农业出版社,2007:122-140
    WEI J C. Manual of fungal identification[M]. Shanghai:Shanghai Scientific and Technical Publishers,1979:141-159 魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社,1979:141-159
    JANG N,WANG Y P,XUE H,et al. A new species of Clonostachys from frozen soil in mount Qomolangma, China[J/OL]. https://link.cnki.net/doi/10.13341/j.jfr.2024.1804, 2025-01-03/2025-05-30 姜宁,王永鹏,薛寒,等. 采自珠穆朗玛峰冻土的螺旋聚孢霉属1新种[J/OL]. https://link.cnki.net/doi/10.13341/j.jfr.2024.1804, 2025-01-03/2025-05-30
    LI H,ZHU H X. Isolation and identification of Alternaria alternata strain HY-063 and its herbicidal potential[J]. Plant Protection,2022,48(6):289-297,325 李欢,朱海霞. 链格孢菌株HY-063的分离鉴定及除草潜力[J]. 植物保护,2022,48(6):289-297,325
    ZHU H X. Study on herbicidal activity and biological characteristics of Epicoccum strain DT-014[J]. Agricultural Research in the Arid Areas,2024,42(1):237-241,251 朱海霞. 附球属真菌DT-014对杂草的致病性及生物学特性研究[J]. 干旱地区农业研究,2024,42(1):237-241,251
    REICHERT JÚNIOR F W,SCARIOT M A,FORTE C T,et al. New perspectives for weeds control using autochthonous fungi with selective bioherbicide potential[J]. Heliyon,2019,5(5):e01676
    ANDERSEN C B,JØRGENSEN H J L,MANZOTTI A,et al. Seed coating with the fungal biocontrol agent Clonostachys rosea controls clubroot in oilseed rape[R]. International Organisation for Biological Control,2018,136:157-163
    BORGES Á V,SARAIVA R M,MAFFIA L A. Maffia. Biocontrol of gray mold in tomato plants by Clonostachys rosea[J]. Tropical Plant Pathology,2015,40(2):71-76
    SUN Y M,WU H L,ZHANG T T,et al. The growth promoting effect of 5406 actinomycetes on watermelon and the control of watermelon Fusarium wilt[J]. Science Technology and Engineering,2021,21(15):6242-6248 孙于淼,吴慧玲,张涛涛,等. 5406放线菌对西瓜的促生作用及对西瓜枯萎病的防控[J]. 科学技术与工程,2021,21(15):6242-6248
    IQBAL M,DUBEY M,MCEWAN K,et al. Evaluation of Clonostachys rosea for control of plant-parasitic nematodes in soil and in roots of carrot and wheat[J]. Phytopathology,2018,108(1):52-59
    ASKEW D J,LAING M D. An adapted selective medium for the quantitative isolation of Trichoderma species[J]. Plant Pathology,1993,42(5):686–690.
    CHATTERTON S,PUNJA Z K. Chitinase and beta-1,3-glucanase enzyme production by the mycoparasite Clonostachys rosea f. catenulata against fungal plant pathogens[J]. Canadian Journal of Microbiology,2009,55(4):356-367
    GAO Z Y,GAN J E,ZHANG L J,et al. A review and current status of weed control using phytopathogenic microorganisms in China[J]. Chinese Agricultural Science Bulletin,1995,(1):31-34,37 高昭远,干静娥,张立洁,等. 我国利用植物病原微生物防除杂草研究的回顾与现状[J]. 中国农学通报,1995,(1):31-34,37
    NIE Y F,CHENG Z Y,LIU Y F,et al. Conidial production of Nimbya alternantherae SF-193 and its pathogenicity to Alternanthera philoxeroides,2009,25(3):260-266 聂亚锋,陈志谊,刘永锋,等. 假隔链格孢SF-193的产孢特性及其分生孢子对空心莲子草的致病力[J]. 中国生物防治,2009,25(3):260-266
    OU S S,ZHANG S J. Biopesticides and biofertilizers[M]. Beijing:Chemical Industry Press,2019:31-32 欧善生,张慎举. 生物农药与肥料[M]. 北京:化学出版社,2019:31-32
    2026年第34卷第4期
    PDF下载
    123
    25
    引用本文
    BibTeX
    文章信息
    doi: 10.11733/j.issn.1007-0435.2026.04.006
    • 接收时间:2025-06-03
    • 首发时间:2026-09-23
    补充材料
    相关文章
    文章信息
    作者
    出版历史
    • 收稿日期:2025-06-03
    • 修回日期:2025-07-16
    基金
    作者信息
    参考文献
    分享链接
    https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.04.006
    分享至
    全文二维码

    扫描看全文

    引用本文
    BibTeX
    本文的引用情况
    2种不同金属材料的力学参数
    科
    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
    关闭全屏