Article(id=1309573269666226411, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.04.023, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1744905600000, receivedDateStr=2025-04-18, revisedDate=1755792000000, revisedDateStr=2025-08-22, acceptedDate=null, acceptedDateStr=null, onlineDate=1790156198183, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790156198183, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790156198183, creator=13701087609, updateTime=1790156198183, updator=13701087609, issue=Issue{id=1309573247344144835, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='4', pageStart='1137', pageEnd='1556', issueExtLink='null', onlineDate='null', pubDate='1776182400000', pubDateStr='2026-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790156192860, creator='13701087609', updateTime=1790156800047, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575794117144683, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575794117144684, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309573247344144835, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1403, endPage=1412, ext={EN=ArticleExt(id=1309573269989187822, articleId=1309573269666226411, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Integrated Improvement Measures on Soil Enzyme Activity and Microbial Communities of Degraded Meadow Grassland, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To investigate the effects of different composite improvement measures on soil enzyme activities and microbial communities in degraded meadow steppes, this study set up four treatments: root cutting+organic fertilizer+microbial inoculant (YQJ), root cutting+organic fertilizer (YQ), root cutting+foliar fertilizer (YY), and control (CK). High-throughput sequencing was used to determine the community structure and diversity of soil bacteria and fungi.The results showed that the YQJ treatment had a significant effect on increasing the activities of soil catalase, invertase, urease, and alkaline phosphatase, followed by the YY treatment. The composition of dominant bacterial and fungal species among different composite improvement treatments was basically similar, but the relative abundance of dominant fungal community species varied greatly. The YQJ treatment significantly reduced the relative abundance of Basidiomycota; the YY treatment significantly increased the relative abundance of Ascomycota (P<0.05). The root cutting+foliar fertilizer treatment significantly increased the Alpha diversity indices of soil bacteria and fungi (P<0.05). Correlation analysis results showed that soil catalase, urease, and invertase were closely related to changes in the bacterial community; soil urease and aboveground biomass were closely related to changes in the fungal community. In conclusion, composite improvement measures could improve soil enzyme activities and grassland productivity by regulating and improving the structure of soil bacterial and fungal communities, and the root cutting+foliar fertilizer treatment had the best improvement effect., authors=CHI Xiao-xue1, CAO Wei1, WANG Chang1, LU Hai-tao1, WEI Hong1, LI Yan-hong1, LI Xin1, CHEN Di1, authorsList=CHI Xiao-xue, CAO Wei, WANG Chang, LU Hai-tao, WEI Hong, LI Yan-hong, LI Xin, CHEN Di, authorCompany=1 Forestry and Grassland Science Research Institute of Hulunbuir, Hulunbuir, Inner Mongolia 021008, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309573269917884652, articleId=1309573269666226411, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为探究不同复合改良措施对退化草甸草原的土壤酶活性及微生物群落的影响,本文设置切根+有机肥+微生物菌剂(YQJ)、切根+有机肥(YQ)、切根+叶面肥(YY)和对照(CK)4种处理,利用高通量测序法测定土壤细菌和真菌的群落结构及多样性。结果显示,YQJ处理对土壤过氧化氢酶、蔗糖酶、脲酶和碱性磷酸酶活性提升效果较为显著,其次是YY处理;不同复合改良措施处理间的细菌和真菌优势菌组成基本相似,但真菌群落优势菌相对丰度差异较大,YQJ处理显著降低了担子菌门(Basidiomycota)的相对丰度;YY处理显著提高了子囊菌门(Ascomycota)相对丰度(P<0.05)。切根+叶面肥处理显著提高土壤细菌和真菌的Alpha多样性指数(P<0.05)。相关性分析结果显示,土壤过氧化氢酶、脲酶和蔗糖酶与细菌群落变化密切相关;土壤脲酶和地上生物量与真菌群落变化密切相关。综上,复合改良措施通过调控和改善土壤细菌和真菌群落结构,提高土壤酶活性和草地生产力,且切根+叶面肥处理改良效果最佳。, authors=迟晓雪1, 曹薇1, 王畅1, 鲁海涛1, 魏红1, 李艳红1, 李欣1, 陈頔1, authorsList=迟晓雪, 曹薇, 王畅, 鲁海涛, 魏红, 李艳红, 李欣, 陈頔, authorCompany=1 呼伦贝尔市林业和草原科学研究所, 内蒙古 呼伦贝尔 021008, correspAuthors=null, authorNote=迟晓雪(1986-),女,汉族,内蒙古呼伦贝尔人,副研究员,主要从事草原生态修复研究,E-mail:chixiaoxue.333@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=BpOdxkTPppKapI+Hntk90A==, pdfFileSize=1852746, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=内蒙古自治区重点研发与成果转化项目 (2022YFDZ0040); 呼伦贝尔市科技计划项目 (SF2023006); 呼伦贝尔市科技计划项目 (ST2024002); 呼伦贝尔市科技计划项目 (SF2025001))}, authors=null, keywords=[Keyword(id=1309573270135988463, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573269666226411, language=CN, orderNo=1, keyword=切根), Keyword(id=1309573270203097328, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309573269666226411, language=CN, orderNo=2, keyword=施肥), Keyword(id=1309573270274400497, 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复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响
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草地学报 | 研究论文 2026,34(4): 1403-1412
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草地学报 |研究论文 2026 , 34 (4) : 1403 -1412
复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响
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迟晓雪1, 曹薇1, 王畅1, 鲁海涛1, 魏红1, 李艳红1, 李欣1, 陈頔1
作者信息
    1 呼伦贝尔市林业和草原科学研究所, 内蒙古 呼伦贝尔 021008
作者简介:
迟晓雪(1986-),女,汉族,内蒙古呼伦贝尔人,副研究员,主要从事草原生态修复研究,E-mail:chixiaoxue.333@163.com
Effects of Integrated Improvement Measures on Soil Enzyme Activity and Microbial Communities of Degraded Meadow Grassland
  • CHI Xiao-xue1, CAO Wei1, WANG Chang1, LU Hai-tao1, WEI Hong1, LI Yan-hong1, LI Xin1, CHEN Di1
  • Affiliations
      1 Forestry and Grassland Science Research Institute of Hulunbuir, Hulunbuir, Inner Mongolia 021008, China
    doi: 10.11733/j.issn.1007-0435.2026.04.023
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    为探究不同复合改良措施对退化草甸草原的土壤酶活性及微生物群落的影响,本文设置切根+有机肥+微生物菌剂(YQJ)、切根+有机肥(YQ)、切根+叶面肥(YY)和对照(CK)4种处理,利用高通量测序法测定土壤细菌和真菌的群落结构及多样性。结果显示,YQJ处理对土壤过氧化氢酶、蔗糖酶、脲酶和碱性磷酸酶活性提升效果较为显著,其次是YY处理;不同复合改良措施处理间的细菌和真菌优势菌组成基本相似,但真菌群落优势菌相对丰度差异较大,YQJ处理显著降低了担子菌门(Basidiomycota)的相对丰度;YY处理显著提高了子囊菌门(Ascomycota)相对丰度(P<0.05)。切根+叶面肥处理显著提高土壤细菌和真菌的Alpha多样性指数(P<0.05)。相关性分析结果显示,土壤过氧化氢酶、脲酶和蔗糖酶与细菌群落变化密切相关;土壤脲酶和地上生物量与真菌群落变化密切相关。综上,复合改良措施通过调控和改善土壤细菌和真菌群落结构,提高土壤酶活性和草地生产力,且切根+叶面肥处理改良效果最佳。
    切根  /  施肥  /  退化草地  /  土壤酶活性  /  细菌和真菌多样性
    To investigate the effects of different composite improvement measures on soil enzyme activities and microbial communities in degraded meadow steppes, this study set up four treatments: root cutting+organic fertilizer+microbial inoculant (YQJ), root cutting+organic fertilizer (YQ), root cutting+foliar fertilizer (YY), and control (CK). High-throughput sequencing was used to determine the community structure and diversity of soil bacteria and fungi.The results showed that the YQJ treatment had a significant effect on increasing the activities of soil catalase, invertase, urease, and alkaline phosphatase, followed by the YY treatment. The composition of dominant bacterial and fungal species among different composite improvement treatments was basically similar, but the relative abundance of dominant fungal community species varied greatly. The YQJ treatment significantly reduced the relative abundance of Basidiomycota; the YY treatment significantly increased the relative abundance of Ascomycota (P<0.05). The root cutting+foliar fertilizer treatment significantly increased the Alpha diversity indices of soil bacteria and fungi (P<0.05). Correlation analysis results showed that soil catalase, urease, and invertase were closely related to changes in the bacterial community; soil urease and aboveground biomass were closely related to changes in the fungal community. In conclusion, composite improvement measures could improve soil enzyme activities and grassland productivity by regulating and improving the structure of soil bacterial and fungal communities, and the root cutting+foliar fertilizer treatment had the best improvement effect.
    Root cutting  /  Fertilization  /  Degraded grassland  /  Soil enzyme activity  /  Bacterial and fungal diversity
    迟晓雪, 曹薇, 王畅, 鲁海涛, 魏红, 李艳红, 李欣, 陈頔. 复合改良措施对退化草甸草原土壤酶活性与微生物群落的影响. 草地学报, 2026 , 34 (4) : 1403 -1412 . DOI: 10.11733/j.issn.1007-0435.2026.04.023
    CHI Xiao-xue, CAO Wei, WANG Chang, LU Hai-tao, WEI Hong, LI Yan-hong, LI Xin, CHEN Di. Effects of Integrated Improvement Measures on Soil Enzyme Activity and Microbial Communities of Degraded Meadow Grassland[J]. Acta Agrestia Sinica, 2026 , 34 (4) : 1403 -1412 . DOI: 10.11733/j.issn.1007-0435.2026.04.023

      内蒙古自治区重点研发与成果转化项目 (2022YFDZ0040); 呼伦贝尔市科技计划项目 (SF2023006); 呼伦贝尔市科技计划项目 (ST2024002); 呼伦贝尔市科技计划项目 (SF2025001)

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    2种不同金属材料的力学参数
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    种数
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    species
    占总种数比例
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    total species (%)
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    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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