Article(id=1309569831066764135, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.03.004, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1743350400000, receivedDateStr=2025-03-31, revisedDate=1748188800000, revisedDateStr=2025-05-26, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155378357, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155378357, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155378357, creator=13701087609, updateTime=1790155378357, updator=13701087609, issue=Issue{id=1309569821424051089, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='3', pageStart='745', pageEnd='1136', issueExtLink='null', onlineDate='null', pubDate='1773504000000', pubDateStr='2026-03-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155376058, creator='13701087609', updateTime=1790156773348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575682133422151, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575682133422152, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569821424051089, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=779, endPage=787, ext={EN=ArticleExt(id=1309569831519748969, articleId=1309569831066764135, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Analysis of Genetic Diversity of Different Geographic Populations of Eospalax baileyi Based on MHC-DRB Exon 2 Gene, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=To elucidate the genetic diversity and adaptive evolutionary mechanisms of different geographical populations of the plateau zokor (Eospalax baileyi), in this study PCR was conducted to amplify and clone the MHC-DRB gene exon 2 in 121 individuals from eight distinct geographical populations. The results showed that the MHC-DRB exon 2 of the plateau zokor exhibits high polymorphism, with significant genetic differentiation among populations. Geographical isolation was identified as the primary factor driving population divergence. Codon usage bias was observed in nucleotide composition. Neutral tests indicated that the plateau zokor population had not undergone recent expansion, and the maintenance of genetic polymorphism was primarily driven by natural selection. The findings of this study will provide a theoretical basis for further investigating the genetic adaptation of MHC genes in Eospalax baileyi and their effective population management., authors=GONG Jun-qiang1,2, HOU Qi-qi1,2, OU Ke-jie1,2, HAO Biao-biao1,2, WANG Wen-bi1,2, SU Jun-hu1,2,3, authorsList=GONG Jun-qiang, HOU Qi-qi, OU Ke-jie, HAO Biao-biao, WANG Wen-bi, SU Jun-hu, authorCompany=1 School of Grassland, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education of Gansu Province/Gansu Provincial Grassland Engineering Laboratory/Center for Sustainable Development of Grassland Animal Husbandry of China and the United States, Lanzhou, Gansu Province 730070, China;
2 Center for Grassland Biodiversity Research, Gansu Agricultural University-Mercy University, Lanzhou, Gansu Province 730070, China;
3 Gansu Qilianshan Grassland Ecosystem Observation and Research Station, Wuwei, Gansu Province733200, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569831398114152, articleId=1309569831066764135, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=高原鼢鼠不同地理种群MHC-DRB基因外显子2遗传多样性分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了解高原鼢鼠(Eospalax baileyi)不同地理种群的遗传多样性和适应性进化机制,本文对高原鼢鼠8个不同地理种群121只个体的MHC-DRB基因外显子2进行PCR扩增及克隆。结果发现,高原鼢鼠MHC-DRB基因外显子2具有较高的多态性,种群间遗传分化明显,地理隔离是各种群分化的关键因素,碱基使用方面存在密码子偏好性,中性检测表明高原鼢鼠种群近期未发生过种群扩张现象,且维持遗传多态性的驱动力主要为选择作用。本研究结果将为进一步研究高原鼢鼠的MHC基因的遗传适应性和种群有效管理提供理论依据。, authors=龚俊强1,2, 侯齐琪1,2, 欧克杰1,2, 郝彪彪1,2, 王文碧1,2, 苏军虎1,2,3, authorsList=龚俊强, 侯齐琪, 欧克杰, 郝彪彪, 王文碧, 苏军虎, authorCompany=1 甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070;
2 甘肃农业大学-新西兰梅西大学草地生物多样性研究中心, 甘肃 兰州 730070;
3 甘肃祁连山草原生态系统观测研究站, 甘肃 武威 733200, correspAuthors=null, authorNote=龚俊强(1999-),男,汉族,甘肃宕昌人,硕士研究生,主要从事啮齿动物生态学与草地保护方面研究,E-mail:1741658013@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=ErHSQWyoE8dGkPmFTdq3og==, pdfFileSize=1081886, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=甘肃省重点研发生态文明建设专项 (24YFFA058); 甘肃省草原健康和退化评估科技支撑项目 (GSAU-JSFW-2024-139-1); 国家自然科学基金项目 (32272566))}, authors=null, keywords=[Keyword(id=1309569831796573034, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569831066764135, language=CN, orderNo=1, keyword=高原鼢鼠), Keyword(id=1309569831884653419, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569831066764135, language=CN, orderNo=2, 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WANG C,PAN Y T,WANG J,et al. Selection of plateau zokor(Eospalax baileyi) DNA barcodes[J]. Pratacultural Science,2020,37(12):2574-2583王缠,潘永泰,王静,等. 高原鼢鼠DNA条形码筛选[J]. 草业科学,2020,37(12):2574-2583
SU J H,WEIHONGJI,NAN Z B,et al. Review on the systematics of the subfamily mysopalacinae[J]. Chinese Journal of Zoology,2015,50(4):649-658苏军虎,WeihongJi,南志标,等. 鼢鼠亚科Mysopalacinae动物系统学研究现状与展望[J]. 动物学杂志,2015,50(4):649-658
KANG Y K,LIU Q Q,YAO B H,et al. Pleistocene climate-driven diversification of plateau zokor (Eospalax baileyi) in the eastern Qinghai-Tibet Plateau[J]. Ecological Indicators,2022,139:108923
DOBSON F S. Measures of gene flow in the Columbian ground squirrel[J]. Oecologia,1994,100(1):190-195
HORN P L,RAFALSKI J A,WHITEHEAD P J. Molecular genetic (RAPD) analysis of breeding magpie geese[J]. The Auk,1996,113(3):552-557
CHEN F F,PAN L,GEN Z Y,et al. Origin,evolvement and resistance mechanism of polymorphism of MHC molecules[J]. Acta Veterinaria et Zootechnica Sinica,2010,41(9):1061-1067陈芳芳,潘玲,耿照玉,等. MHC分子多态性的起源、演变与抗病机理[J]. 畜牧兽医学报,2010,41(9):1061-1067
SU J H,LIU R T,JI W H,et al. Stages and characteristics of grassland rodent pests control and research in China[J]. Pratacultural Science,2013,30(7):1116-1123苏军虎,刘荣堂,纪维红,等. 我国草地鼠害防治与研究的发展阶段及特征[J]. 草业科学,2013,30(7):1116-1123
HIRSCH F,SACHS D H,GUSTAFSSON K,et al. Class II genes of miniature swine. III. Characterization of an expressed pig class II gene homologous to HLA-DQA[J]. Immunogenetics,1990,31(1):52-56
HUGHES A L,NEI M. Pattern of nucleotide substitution at major histocompatibility complex class I loci reveals overdominant selection[J]. Nature,1988,335(6186):167-170
SHI L B,WANG Y,ZOU J X,et al. Genetic differentiation of S.fukienense and S.xiusuiense based on 16S rRNA[J]. Chinese Journal of Zoonoses,2012,28(3):213-216,222石林波,汪雁,邹节新,等. 基于16S rRNA初步探讨福建华溪蟹与修水华溪蟹间的遗传分化[J]. 中国人兽共患病学报,2012,28(3):213-216,222
CAI Z Y,ZHANG T Z,CI H X,et al. Mitochondrial phylogeography and genetic diversity of plateau zokor(Myospalax baileyi) [J]. Acta Theriologica Sinica, 2007,27(2):130-137蔡振媛,张同作,慈海鑫,等. 高原鼢鼠线粒体谱系地理学和遗传多样性[J]. 兽类学报,2007,27(2):130-137
WRIGHT S. Isolation by distance[J]. Genetics,1943,28(2):114-138
TANG L Z,ZHANG T Z,LIN G H,et al. Phylogenetic discontinuity of plateau zokor (Myospalax Baileyi Thomas) populations in Qinghai-Tibetan Plateau[J]. Polish Journal of Ecology,2010,58(1):167-176
QUAX T E F,CLAASSENS N J,SÖLL D,et al. Codon bias as a means to fine-tune gene expression[J]. Molecular Cell,2015,59(2):149-161
PAN X,FU J. Molecular evolution of MHC DQA genes. I. The maintenance of interallelic divergence and the influence of GC content on gene structure[J]. Acta Genetica Sinica,1997,24(3):195-205
MA J Q,YANG Q F,SONG Z F,et al. Analysis of Codon usage bias of VDR gene related to bone metabolism in Ovis aries[J]. China Animal Husbandry & Veterinary Medicine,2022,49(8):2888-2898马建青,杨清芳,宋占锋,等. 绵羊骨代谢相关基因VDR密码子偏好性分析[J]. 中国畜牧兽医,2022,49(8):2888-2898
CAMMEN K,HOFFMAN J I,KNAPP L A,et al. Geographic variation of the major histocompatibility complex in Eastern Atlantic grey seals (Halichoerus grypus)[J]. Molecular Ecology,2011,20(4):740-752
BLANTON R E,PAGE L M,HILBER S A. Timing of clade divergence and discordant estimates of genetic and morphological diversity in the Slender Madtom,Noturus exilis (Ictaluridae)[J]. Molecular Phylogenetics and Evolution,2013,66(3):679-693
LIANG J,ZHOU L Z,ZHAO T B,et al. Genetic variation and geographicial differentiation of Cytochrome b gene of Clawed jird (Meriones unguiculatus)[J]. Acta Theriologica Sinica,2007,27(2):138-145梁君,周立志,赵天飙,等. 长爪沙鼠线粒体细胞色素b基因的遗传变异及地理分化[J]. 兽类学报,2007,27(2):138-145
ZHAO D R,SUN L L. Formation of MHC polymorphism in mammals[J]. International Journal of Genetics,1995,18(6):330-332赵德容,孙岚玲. 哺乳类MHC多态性的形成[J]. 国外医学遗传学分册,1995,18(6):330-332)
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草地学报 |研究论文 2026 , 34 (3) : 779 -787
高原鼢鼠不同地理种群MHC-DRB基因外显子2遗传多样性分析
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龚俊强1,2, 侯齐琪1,2, 欧克杰1,2, 郝彪彪1,2, 王文碧1,2, 苏军虎1,2,3
作者信息
    1 甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070;
    2 甘肃农业大学-新西兰梅西大学草地生物多样性研究中心, 甘肃 兰州 730070;
    3 甘肃祁连山草原生态系统观测研究站, 甘肃 武威 733200
作者简介:
龚俊强(1999-),男,汉族,甘肃宕昌人,硕士研究生,主要从事啮齿动物生态学与草地保护方面研究,E-mail:1741658013@qq.com
Analysis of Genetic Diversity of Different Geographic Populations of Eospalax baileyi Based on MHC-DRB Exon 2 Gene
  • GONG Jun-qiang1,2, HOU Qi-qi1,2, OU Ke-jie1,2, HAO Biao-biao1,2, WANG Wen-bi1,2, SU Jun-hu1,2,3
  • Affiliations
      1 School of Grassland, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education of Gansu Province/Gansu Provincial Grassland Engineering Laboratory/Center for Sustainable Development of Grassland Animal Husbandry of China and the United States, Lanzhou, Gansu Province 730070, China;
      2 Center for Grassland Biodiversity Research, Gansu Agricultural University-Mercy University, Lanzhou, Gansu Province 730070, China;
      3 Gansu Qilianshan Grassland Ecosystem Observation and Research Station, Wuwei, Gansu Province733200, China
    doi: 10.11733/j.issn.1007-0435.2026.03.004
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    为了解高原鼢鼠(Eospalax baileyi)不同地理种群的遗传多样性和适应性进化机制,本文对高原鼢鼠8个不同地理种群121只个体的MHC-DRB基因外显子2进行PCR扩增及克隆。结果发现,高原鼢鼠MHC-DRB基因外显子2具有较高的多态性,种群间遗传分化明显,地理隔离是各种群分化的关键因素,碱基使用方面存在密码子偏好性,中性检测表明高原鼢鼠种群近期未发生过种群扩张现象,且维持遗传多态性的驱动力主要为选择作用。本研究结果将为进一步研究高原鼢鼠的MHC基因的遗传适应性和种群有效管理提供理论依据。
    高原鼢鼠  /  MHC-DRB外显子2  /  多态性  /  遗传多样性  /  选择作用
    To elucidate the genetic diversity and adaptive evolutionary mechanisms of different geographical populations of the plateau zokor (Eospalax baileyi), in this study PCR was conducted to amplify and clone the MHC-DRB gene exon 2 in 121 individuals from eight distinct geographical populations. The results showed that the MHC-DRB exon 2 of the plateau zokor exhibits high polymorphism, with significant genetic differentiation among populations. Geographical isolation was identified as the primary factor driving population divergence. Codon usage bias was observed in nucleotide composition. Neutral tests indicated that the plateau zokor population had not undergone recent expansion, and the maintenance of genetic polymorphism was primarily driven by natural selection. The findings of this study will provide a theoretical basis for further investigating the genetic adaptation of MHC genes in Eospalax baileyi and their effective population management.
    Eospalax baileyi  /  MHC-DRB exon 2  /  Polymorphism  /  Genetic diversity  /  Selective effect
    龚俊强, 侯齐琪, 欧克杰, 郝彪彪, 王文碧, 苏军虎. 高原鼢鼠不同地理种群MHC-DRB基因外显子2遗传多样性分析. 草地学报, 2026 , 34 (3) : 779 -787 . DOI: 10.11733/j.issn.1007-0435.2026.03.004
    GONG Jun-qiang, HOU Qi-qi, OU Ke-jie, HAO Biao-biao, WANG Wen-bi, SU Jun-hu. Analysis of Genetic Diversity of Different Geographic Populations of Eospalax baileyi Based on MHC-DRB Exon 2 Gene[J]. Acta Agrestia Sinica, 2026 , 34 (3) : 779 -787 . DOI: 10.11733/j.issn.1007-0435.2026.03.004

      甘肃省重点研发生态文明建设专项 (24YFFA058); 甘肃省草原健康和退化评估科技支撑项目 (GSAU-JSFW-2024-139-1); 国家自然科学基金项目 (32272566)

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    doi: 10.11733/j.issn.1007-0435.2026.03.004
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    2种不同金属材料的力学参数
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    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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