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2 Inner Mongolia Baotou No. 40 Middle School, Baotou, Inner Mongolia 014030, China;
3 Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, Hohhot, Inner Mongolia 010070, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569828864750347, articleId=1309569828143330058, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=黄花苜蓿MfWRKY3基因克隆及特性分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了探究MfWRKY3在调节黄花苜蓿（Medicago falcata）响应逆境胁迫过程中发挥的作用，本研究从野生黄花苜蓿中克隆获得了MfWRKY3基因，并对其编码蛋白特性和表达模式进行了研究。结果发现，MfWRKY3基因开放阅读框为1575 bp，编码524个氨基酸的蛋白质，其分子量为57.36 kDa，理论等电点为6.96。MfWRKY3蛋白具有转录激活特性并定位在细胞核内，属于WRKY Ⅰ亚家族转录因子家族成员。MfWRKY3基因在黄花苜蓿叶中表达量最高，且在根、茎和叶中的表达均受盐和低温胁迫以及脱落酸（Abscisic acid，ABA）处理的影响。其中，盐胁迫可显著诱导MfWRKY3基因的表达。这些结果为进一步探究MfWRKY3基因调节黄花苜蓿响应逆境胁迫的生物学功能和机理奠定了基础。, authors=赵静玮^1,2, 金宵溦¹, 崔梦兰¹, 张立全^1,3, authorsList=赵静玮, 金宵溦, 崔梦兰, 张立全, authorCompany=1 内蒙古大学生命科学学院, 内蒙古 呼和浩特 010070;
2 内蒙古包头市第四十中学, 内蒙古 包头 014030;
3 牧草与特色作物生物学教育部重点实验室, 内蒙古 呼和浩特 010070, correspAuthors=null, authorNote=赵静玮（1995-），女，汉族，内蒙古包头人，硕士研究生，主要从事苜蓿抗逆方面研究，E-mail：1120470879@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=ZLSUfeXI/uC1/h7JAnfxFA==, pdfFileSize=4474948, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金地区项目 (32460339,31460628); 内蒙古自然科学基金面上项目 (2024MS03028); 内蒙古自治区高等学校科学技术研究重点项目 (NJZZ22331))}, authors=null, keywords=[Keyword(id=1309569830521500429, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569828143330058, language=CN, orderNo=1, keyword=黄花苜蓿), Keyword(id=1309569830605386510, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569828143330058, language=CN, orderNo=2, 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