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By measuring seven key indicators， it was found that the contents of malondialdehyde （MDA）， soluble sugar （SS）， and proline （Pro）， as well as the level of initial fluorescence （F_o）， exhibited upward trends with decreasing temperature， all significantly increasing at 0℃ （P<0.05）； while the relative chlorophyll content （Soil and plant analyzer development， SPAD）， maximum fluorescence （F_m）， and maximum photochemical efficiency of PSⅡ （Maximal photochemical efficiency of photosystem II， F_v/F_m） showed downward trends. A comprehensive evaluation using the membership function method combined with the standard deviation coefficient weighting method identified M₄， M₁， and M₁₀ as accessions with stronger cold tolerance. Stepwise regression analysis further established a cold tolerance prediction model （R²=0.932）， confirming F_v/F_m and SS content as critical evaluation indices. These results provide excellent germplasm resources and a theoretical basis for cold-tolerance breeding of alfalfa， and it is recommended that future research integrate omics technologies to further elucidate the underlying molecular regulatory networks., authors=DUAN Wei¹, MIJIT Munire¹, YIMIN Jamilaigul¹, WANG Yu-xiang¹, LI Qian¹, authorsList=DUAN Wei, MIJIT Munire, YIMIN Jamilaigul, WANG Yu-xiang, LI Qian, authorCompany=1 Key Laboratory of Grassland Resources and Ecology of Western Arid Region Ministry of Education, College of Grassland Science Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569651969974993, articleId=1309569650065760976, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=20份黄花苜蓿种质低温胁迫生理响应及抗寒性评价, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为筛选耐寒型黄花苜蓿（Medicago falcata）材料并阐明其低温生理响应机制，本研究系统评价了20份种质在梯度低温胁迫（25℃，10℃，5℃，0℃）下的生理及叶绿素荧光特性。通过测定7项关键指标发现：随温度降低，丙二醛（Malondialdehyde，MDA）、可溶性糖（Soluble sugar，SS）、脯氨酸（Proline，Pro）含量及初始荧光（Initial fluorescence，Fo）呈上升趋势，在0℃均显著增加（P<0.05）；而叶绿素相对含量（Soil and plant analyzer development，SPAD）、最大荧光（Maximum fluorescence，Fm）和PSⅡ最大光化学效率（Maximal photochemical efficiency of photosystem II，Fv/Fm）呈下降趋势。基于隶属函数法结合标准差系数赋权法的综合评价表明，M₄，M₁，M₁₀等种质表现出较强耐寒性。逐步回归分析进一步建立了耐寒性预测模型（R²=0.932），确定Fv/Fm和SS含量为关键评价指标。研究结果为苜蓿抗寒育种提供了优异种质和理论依据，建议后续结合组学等技术深入解析其分子调控网络。, authors=段伟¹, 穆妮热·米吉提¹, 加米丽古丽·伊明¹, 王玉祥¹, 李倩¹, authorsList=段伟, 穆妮热·米吉提, 加米丽古丽·伊明, 王玉祥, 李倩, authorCompany=1 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