Article(id=1309569644114047423, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.02.008, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1745942400000, receivedDateStr=2025-04-30, revisedDate=1754064000000, revisedDateStr=2025-08-02, acceptedDate=null, acceptedDateStr=null, onlineDate=1790155333784, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790155333784, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790155333784, creator=13701087609, updateTime=1790155333784, updator=13701087609, issue=Issue{id=1309569631556301196, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='2', pageStart='377', pageEnd='744', issueExtLink='null', onlineDate='null', pubDate='1771084800000', pubDateStr='2026-02-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790155330790, creator='13701087609', updateTime=1790156750858, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575588013244561, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575588017438866, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309569631556301196, language=CN, specialIssueTitle=, coverIllustrator=, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=456, endPage=468, ext={EN=ArticleExt(id=1309569644487340481, articleId=1309569644114047423, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Bermudagrass has important application value in landscaping and ecological restoration because of its strong drought resistance, saline-alkali resistance, and developed rhizomes and stolons. However, the molecular mechanism of its response to alkaline salt stress is still unclear. The WRKY gene family plays an important role in plant growth, development and defense against biotic and abiotic stresses, but it has not been fully identified in bermudagrass. Therefore, based on genomic data, this study systematically identified the physical and chemical properties, chromosome distribution, cis-acting elements, gene structure, conserved motifs and phylogenetic trees of WRKY gene family members in bermudagrass. At the same time, based on transcriptome data, the expression pattern of its family members under alkaline salt stress was analyzed. The results showed that 129 WRKY transcription factors were identified in the bermudagrass genome and named according to their location on the chromosome. Phylogenetic analysis was carried out by comparing with Arabidopsis WRKY protein, and the above transcription factors were divided into three families : I, II and III, which were unevenly distributed on 18 chromosomes with the largest number of chromosomes 7 (22).Collinearity analysis showed that fragment replication mainly drove the evolution of CdWRKYs in bermudagrass. Based on the transcriptome data, 11 differentially expressed CdWRKYs under alkaline salt stress were screened out. The qRT-PCR analysis showed that the expression levels of CdWRKY59, CdWRKY81, CdWRKY113 and CdWRKY126 reached the peak at 3 h, and their expression folds were 99.6-312.3 times than the control. It is speculated that these WRKY family members might play an important role in alkaline salt stress tolerance. The results of this study analyzed the role of WRKY genes in the response of bermudagrass to alkaline salt stress, and laid a foundation for further functional analysis of WRKY gene family., authors=MA Shi-rui1, YU Zheng-fa1, YU Qi-kun1, YU Rui1, ZHU Li-li1, ZHANG Zhi-yang1, LI Pei-ying1,2,3, authorsList=MA Shi-rui, YU Zheng-fa, YU Qi-kun, YU Rui, ZHU Li-li, ZHANG Zhi-yang, LI Pei-ying, authorCompany=1 College of Grassland science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China; 2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China; 3 Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang 830052, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309569644399260096, articleId=1309569644114047423, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=狗牙根WRKY基因家族鉴定及碱性盐胁迫下表达分析, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=本研究基于狗牙根(Cynodon dactylon)基因组数据,采用生物信息学方法对其WRKY基因家族成员进行鉴定,同时基于转录组数据分析其在碱性盐胁迫下的表达模式。结果显示,狗牙根基因组中共鉴定出129个WRKY转录因子,根据其在染色体上的位置进行命名。通过与拟南芥WRKY蛋白比对进行系统发育分析,将上述转录因子分为Ⅰ、Ⅱ、Ⅲ共3个族,其不均匀分布在狗牙根18条染色体上,其中7号染色体的数量最多(22个)。共线性分析表明,片段复制主要驱动了狗牙根CdWRKYs的进化。基于转录组数据,筛选出11个碱性盐胁迫下差异表达的CdWRKYs,经qRT-PCR分析表明,推测CdWRKY59、CdWRKY81、CdWRKY113与CdWRKY126可能在狗牙根碱性盐胁迫耐受中具有重要作用。本研究结果可为WRKY基因家族的进一步功能分析奠定基础。, authors=马诗睿1, 于正发1, 喻启坤1, 于瑞1, 朱莉莉1, 张志扬1, 李培英1,2,3, authorsList=马诗睿, 于正发, 喻启坤, 于瑞, 朱莉莉, 张志扬, 李培英, authorCompany=1 新疆农业大学草业学院, 新疆 乌鲁木齐 830052; 2 新疆草地资源与生态自治区重点实验室, 新疆 乌鲁木齐 830052; 3 西部干旱区草地资源与生态教育部重点实验室, 新疆 乌鲁木齐 830052, correspAuthors=null, authorNote=马诗睿(2001-),女,汉族,新疆昌吉人,硕士研究生,主要从事草种抗性分析机制研究,E-mail:1529837814@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=yRxAo1ZWglpLxXsuAjqmNQ==, pdfFileSize=5381379, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金 (32260341); 新疆维吾尔自治区重点研发项目 (2023B02031-1))}, authors=[Author(id=1320569639856353585, tenantId=1146029695717560320, journalId=null, articleId=1309569644114047423, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1308397196074385509, articleId=1309569644114047423, authorId=1320569639856353585, language=EN, stringName=MA Shi-rui, YU Zheng-fa, YU Qi-kun, YU Rui, ZHU Li-li, ZHANG Zhi-yang, LI Pei-ying, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=null, tenantId=null, journalId=1308397196074385509, articleId=1309569644114047423, authorId=1320569639856353585, language=CN, stringName=马诗睿, 于正发, 喻启坤, 于瑞, 朱莉莉, 张志扬, 李培英, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)], keywords=[Keyword(id=1309569644650918338, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=CN, orderNo=1, keyword=狗牙根), Keyword(id=1309569644734804419, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=CN, orderNo=2, keyword=WRKY转录因子), Keyword(id=1309569644810301892, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=CN, orderNo=3, keyword=全基因组鉴定), Keyword(id=1309569644885799365, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=CN, orderNo=4, keyword=碱性盐胁迫), Keyword(id=1309569644961296838, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=CN, orderNo=5, keyword=表达模式), Keyword(id=1309569645082931655, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=EN, orderNo=1, keyword=Bermudagrass), Keyword(id=1309569645166817736, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=EN, orderNo=2, keyword=WRKY transcription factor), Keyword(id=1309569645254898121, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=EN, orderNo=3, keyword=Genome-wide identification), Keyword(id=1309569645330395594, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=EN, orderNo=4, keyword=Alkaline salt stress), Keyword(id=1309569645439447499, tenantId=1146029695717560320, journalId=1308397196074385509, articleId=1309569644114047423, language=EN, orderNo=5, keyword=Expressive mode)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1308395841968504927, delFlag=0, nameCn=草地学报, nameEn=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, issn=1007-0435, eissn=null, cn=11-3362/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=gCdi9sXCX82eLR6S1d4VLg==, journalPrice=null, startedYear=null, abbrevIsoEn=Acta Agrestia Sinica, journalRemark=null, publicationField=null, createdTime=1789875477545, updatedTime=1789876241473, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=gCdi9sXCX82eLR6S1d4VLg==, picEn=4dKqEDfsaGuyf+bpm/3gfw==, jcr=null, cjcr=null, exts=[JournalExt(id=1308399046192812446, language=CN, name=草地学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241491, updatedTime=1789876241491, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1308399046268309919, language=EN, name=Acta Agrestia Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1789876241509, updatedTime=1789876241509, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/authorLogOn.action, submissionEditorUrl=https://manu40.magtech.com.cn/JournalX_cdxb/editorLogOn.action, submissionReviewUrl=https://manu40.magtech.com.cn/JournalX_cdxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1308397196074385509, websiteList=[Website(id=1308429426430071695, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/CN, language=CN, createTime=1789883484704, createBy=18614031015, updateTime=1789883745058, updateBy=18614031015, name=草地学报-中文, tplId=1146099689490845704, title=草地学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430669353340972, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=articleTextType, value=kx, createTime=1789883781040, updateTime=1789883781040, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669328175145, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=banner, value=null, createTime=1789883781034, updateTime=1789883781034, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669382701103, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=grayFlag, value=0, createTime=1789883781047, updateTime=1789883781047, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669319786536, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883781032, updateTime=1789883781032, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669395284017, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=minRunFlag, value=0, createTime=1789883781050, updateTime=1789883781050, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669344952363, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/CN/file/pic, createTime=1789883781038, updateTime=1789883781038, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669391089712, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=silenceFlag, value=0, createTime=1789883781049, updateTime=1789883781049, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669336563754, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1789883781036, updateTime=1789883781036, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669361729581, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeColor, value=null, createTime=1789883781042, updateTime=1789883781042, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430669370118190, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426430071695, code=themeStyle, value=null, createTime=1789883781044, updateTime=1789883781044, creator=18614031015, updator=18614031015)]), Website(id=1308429426501374870, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1308397196074385509, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/cdxb/EN, language=EN, createTime=1789883484721, createBy=18614031015, updateTime=1789883716139, updateBy=18614031015, name=草地学报-英文, tplId=1146101810881728533, title=Acta Agrestia Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1308430635979264030, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=articleTextType, value=kx, createTime=1789883773083, updateTime=1789883773083, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635954098203, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=banner, value=null, createTime=1789883773077, updateTime=1789883773077, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636000235553, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=grayFlag, value=0, createTime=1789883773088, updateTime=1789883773088, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635945709594, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=logo, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic?fileId=BZ28lIoKdB54MmA0z6K66g==, createTime=1789883773075, updateTime=1789883773075, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636012818467, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=minRunFlag, value=0, createTime=1789883773091, updateTime=1789883773091, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635970875421, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/cdxb/EN/file/pic, createTime=1789883773081, updateTime=1789883773081, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430636004429858, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=silenceFlag, value=0, createTime=1789883773089, updateTime=1789883773089, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635962486812, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1789883773079, updateTime=1789883773079, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635987652639, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeColor, value=null, createTime=1789883773085, updateTime=1789883773085, creator=18614031015, updator=18614031015), WebsiteProps(id=1308430635991846944, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1308429426501374870, code=themeStyle, value=null, createTime=1789883773086, updateTime=1789883773086, creator=18614031015, updator=18614031015)])], journalTitle=草地学报, weixinUrl=null, journalUrl=https://manu40.magtech.com.cn/Jweb_cdxb, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Agrestia Sinica, journalPhotoCn=gCdi9sXCX82eLR6S1d4VLg==, journalPhotoEn=4dKqEDfsaGuyf+bpm/3gfw==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/10.11733/j.issn.1007-0435.2026.02.008, detailUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/10.11733/j.issn.1007-0435.2026.02.008, pdfUrlCn=https://castjournals.cast.org.cn/joweb/cdxb/CN/PDF/10.11733/j.issn.1007-0435.2026.02.008, pdfUrlEn=https://castjournals.cast.org.cn/joweb/cdxb/EN/PDF/10.11733/j.issn.1007-0435.2026.02.008, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1771084800000, fullTextJson=null, articleText=null, reference=CHAN Z L,SHI H T,WANG Y P. Response of Bermuda grass to abiotic stress[J]. Pratacultural Science,2013,30(8):1182-1187 产祝龙,施海涛,王艳平. 狗牙根抗非生物胁迫的研究进展[J]. 草业科学,2013,30(8):1182-1187 EI B,SHI D S,YANG G,et al. Preliminary research report on the native Cynodon dactylon in Xinjiang[J]. Journal of Xinjiang Agricultural University,1998,21(2):124-127 阿不来提,石定燧,杨光,等. 新疆野生狗牙根研究初报[J]. 新疆农业大学学报,1998,21(2):124-127 HARLAN J R , WET J M J D , RAWAL K M . Cytogenetic studies in Cynodon L. C. rich. (Gramineae)[J] East African Agricultural and Forestry Journal, 1970,10(3): 220-226 LI S,LI P Y,LI W,et al. Effect of drought stress on proline and betaine metabolic pathway in Cynodon dactylon[J]. Chinese Journal of Grassland,2024,46(8):45-52 李硕,李培英,李雯,等. 干旱胁迫下不同抗性狗牙根脯氨酸和甜菜碱代谢变化[J]. 中国草地学报,2024,46(8):45-52 ZHU S B,WUERGULI T,TANG G M,et al. Research progress on saline-alkali land changes and its treatment measures in Xinjiang[J]. Shandong Agricultural Sciences,2023,55(3):158-165 朱生堡,乌尔古丽·托尔逊,唐光木,等. 新疆盐碱地变化及其治理措施研究进展[J]. 山东农业科学,2023,55(3):158-165 CHEN J B,LI D D,HAO D L,et al. Effects of pH value and nitrogen form on growth and sodium and potassium ion regulation of Cynodon dactylon under NaCl stress[J]. Journal of Plant Resources and Environment,2024,33(2):30-40 陈静波,李丹丹,郝东利,等. NaCl胁迫下pH值和氮素形态对狗牙根生长及钠钾离子调控的影响[J]. 植物资源与环境学报,2024,33(2):30-40 ISHIGURO S,NAKAMURA K. Characterization of a cDNA encoding a novel DNA-binding protein,SPF1 that recognizes SP8 sequences in the 5’ upstream regions of genes coding for sporamin and β-amylase from sweet potato[J]. Molecular and General Genetics MGG,1994,244(6):563-571 WANG Q S,WANG M H,ZHANG X Z,et al. WRKY gene family evolution in Arabidopsis thaliana[J]. Genetica,2011,139(8):973-983 EULGEM T,RUSHTON P J,ROBATZEK S,et al. The WRKY superfamily of plant transcription factors[J]. Trends in Plant Science,2000,5(5):199-206 FLOWERS T J,MUNNS R,COLMER T D. Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes[J]. Annals of Botany,2015,115(3):419-431 WANG R Y,WEN W W,ZHAO E H,et al. Cloning of alfalfa MsWRKY11 gene and analysis of its salt tolerance function[J]. Acta Prataculturae Sinica,2021,30(11):157-169 王如月,文武武,赵恩华,等. 紫花苜蓿MsWRKY11基因的克隆及其耐盐功能分析[J]. 草业学报,2021,30(11):157-169 YU Y A,WU Y X,HE L Y. A wheat WRKY transcription factor TaWRKY17 enhances tolerance to salt stress in transgenic Arabidopsis and wheat plant[J]. Plant Molecular Biology,2023,113(4):171-191 HUANG S Z,HU L J,ZHANG S H,et al. Rice OsWRKY50 mediates ABA-dependent seed germination and seedling growth,and ABA-independent salt stress tolerance[J]. International Journal of Molecular Sciences,2021,22(16):8625 SHAO A,XU X,AMOMBO E,et al. CdWRKY2 transcription factor modulates salt oversensitivity in bermudagrass [Cynodon dactylon (L.) Pers[J]. Frontiers in Plant Science,2023,14:1164534 HUANG X B,AMEE M,CHEN L. Bermudagrass CdWRKY50 gene negatively regulates plants’ response to salt stress[J]. Environmental and Experimental Botany,2021,188:104513 CHEN C J,CHEN H,ZHANG Y,et al. TBtools: an integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant,2020,13(8):1194-1202 GUIXIA XU C G. Divergence of duplicate genes in exon-intron structure[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(4):1187-1192 SZKLARCZYK D,GABLE A L,LYON D,et al. STRING v11: protein–protein association networks with increased coverage,supporting functional discovery in genome-wide experimental datasets[J]. Nucleic Acids Research,2019,47(D1):D607-D613 TANG L S. Morphological,physiological and molecular response mechanism of Cynodon dactylon in Xinjiang under alkaline salt stress[D]. Urumqi:Xinjiang Agricultural University,2024:7-18 汤丽斯. 碱性盐胁迫下新疆狗牙根形态、生理及分子响应机制研究[D]. 乌鲁木齐:新疆农业大学,2024:7-18 TANG L S,YU Q K,LI W,et al. Evaluation and identification of reference genes for qRT-PCR analysis in bermudagrass roots under alkaline salt stress[J]. Physiology and Molecular Biology of Plants,2025,31(5):729-738 ROSS C A,LIU Y,SHEN Q J. The WRKY gene family in rice (Oryza sativa)[J]. Journal of Integrative Plant Biology,2007,49(6):827-842 JIANG M,YIN L F,ZHANG Z X,et al. Identification and analysis of WRKY gene family members of Brachypodium bicolor[J]. Journal of Triticeae Crops,2012,32(6):1013-1020 蒋明,尹龙飞,张志仙,等. 二穗短柄草WRKY基因家族成员的鉴定和分析[J]. 麦类作物学报,2012,32(6):1013-1020 LI X Y,LIU H,HE F,et al. Identification and expression pattern of the WRKY transcription factor family in Medicago sativa[J]. Acta Prataculturae Sinica,2024,33(4):154-170 李显炀,刘昊,何飞,等. 全基因组水平紫花苜蓿WRKY转录因子家族鉴定与表达模式分析[J]. 草业学报,2024,33(4):154-170 MAO C Z,WANG L,KONG D D,et al. Bioinformatics analysis of WRKY gene family in barley genome[J]. Molecular Plant Breeding,2019,17(23):7623-7633 毛成志,王蕾,孔豆豆,等. 大麦全基因组WRKY基因家族生物信息学分析[J]. 分子植物育种,2019,17(23):7623-7633 CHEN Q,XU X Y,WANG J C,et al. Identification of a WRKY gene family based on full-length transcriptome sequences and analysis of response patterns under salt stress in Halogeton glomeratus[J]. Acta Prataculturae Sinica,2022,31(12):146-157 陈倩,徐晓芸,汪军成,等. 基于全长转录组的盐生草WRKY基因家族的鉴定及其盐胁迫响应模式分析[J]. 草业学报,2022,31(12):146-157 SHUI D J,SUN J,XIONG Z L,et al. Identification and pathogenic response of tomato WRKY transcription factors[J]. Fujian Journal of Agricultural Sciences,2023,38(3):281-293 水德聚,孙继,熊自立,等. 番茄WRKY转录因子比较鉴定及细菌胁迫响应[J]. 福建农业学报,2023,38(3):281-293 CHEN X J,LI C,WANG H,et al. WRKY transcription factors: evolution,binding,and action[J]. Phytopathology Research,2019,1(1):13 JIANG Y Q,DEYHOLOS M K. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses[J]. Plant Molecular Biology,2009,69(1):91-105 LIU W,JIANG L P,CHI Y J,et al. Identification and expression analysis of WRKY gene family in Coptis chinensis[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2022,24(4):1369-1380 刘微,蒋莉萍,池玉洁,等. 黄连WRKY基因家族鉴定及表达分析[J]. 世界科学技术-中医药现代化,2022,24(4):1369-1380 CANNON S B,MITRA A,BAUMGARTEN A,et al. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana[J]. BMC Plant Biology,2004,4:10 ZHANG S S,RUAN S M,YANG W Z. Identification of WRKY gene family and analysis of drought stress expression in Poncirus Polyandra[J]. Journal of Fruit Science,2025,42(3):508-525 张珊珊,阮孙美,杨文忠. 富民枳WRKY基因家族的鉴定和干旱胁迫表达分析[J]. 果树学报,2025,42(3):508-525 YANG J T,LI P F,XIAO Q Q. Identification and functional analysis of WRKY gene family of Lonicera japonica thunb[J]. Journal of Southwest University (Natural Science Edition),2023,45(7):87-96 杨教童,李鹏飞,肖巧巧. 金银花WRKY基因家族的鉴定与功能分析[J]. 西南大学学报(自然科学版),2023,45(7):87-96 YU Y P,JIANG Y J,SUN X Y,et al. Identification of the WRKY gene family and analysis of its expression in response to drought stress in centipedegrass[J]. Acta Agrestia Sinica,2024,32(5):1378-1391 于元平,蒋宇佳,孙向一,等. 假俭草WRKY基因家族鉴定及其响应干旱胁迫表达分析[J]. 草地学报,2024,32(5):1378-1391 HE F,ZHANG L X,ZHAO G Q,et al. Genome-wide identification and expression analysis of the NAC gene family in alfalfa revealed its potential roles in response to multiple abiotic stresses[J]. International Journal of Molecular Sciences,2022,23(17):10015)
Identification of WRKY Family Genes and Expression Analysis under Alkaline Salt Stress in Bermudagrass
MA Shi-rui, YU Zheng-fa, YU Qi-kun, YU Rui, ZHU Li-li, ZHANG Zhi-yang, LI Pei-ying
Affiliations
1 College of Grassland science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China; 2 Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China; 3 Key Laboratory of Grassland Resources and Ecology of the Ministry of Education of the Western Arid Zone, Urumqi, Xinjiang 830052, China
Bermudagrass has important application value in landscaping and ecological restoration because of its strong drought resistance, saline-alkali resistance, and developed rhizomes and stolons. However, the molecular mechanism of its response to alkaline salt stress is still unclear. The WRKY gene family plays an important role in plant growth, development and defense against biotic and abiotic stresses, but it has not been fully identified in bermudagrass. Therefore, based on genomic data, this study systematically identified the physical and chemical properties, chromosome distribution, cis-acting elements, gene structure, conserved motifs and phylogenetic trees of WRKY gene family members in bermudagrass. At the same time, based on transcriptome data, the expression pattern of its family members under alkaline salt stress was analyzed. The results showed that 129 WRKY transcription factors were identified in the bermudagrass genome and named according to their location on the chromosome. Phylogenetic analysis was carried out by comparing with Arabidopsis WRKY protein, and the above transcription factors were divided into three families : I, II and III, which were unevenly distributed on 18 chromosomes with the largest number of chromosomes 7 (22).Collinearity analysis showed that fragment replication mainly drove the evolution of CdWRKYs in bermudagrass. Based on the transcriptome data, 11 differentially expressed CdWRKYs under alkaline salt stress were screened out. The qRT-PCR analysis showed that the expression levels of CdWRKY59, CdWRKY81, CdWRKY113 and CdWRKY126 reached the peak at 3 h, and their expression folds were 99.6-312.3 times than the control. It is speculated that these WRKY family members might play an important role in alkaline salt stress tolerance. The results of this study analyzed the role of WRKY genes in the response of bermudagrass to alkaline salt stress, and laid a foundation for further functional analysis of WRKY gene family.
CHAN Z L,SHI H T,WANG Y P. Response of Bermuda grass to abiotic stress[J]. Pratacultural Science,2013,30(8):1182-1187 产祝龙,施海涛,王艳平. 狗牙根抗非生物胁迫的研究进展[J]. 草业科学,2013,30(8):1182-1187 EI B,SHI D S,YANG G,et al. Preliminary research report on the native Cynodon dactylon in Xinjiang[J]. Journal of Xinjiang Agricultural University,1998,21(2):124-127 阿不来提,石定燧,杨光,等. 新疆野生狗牙根研究初报[J]. 新疆农业大学学报,1998,21(2):124-127 HARLAN J R , WET J M J D , RAWAL K M . Cytogenetic studies in Cynodon L. C. rich. (Gramineae)[J] East African Agricultural and Forestry Journal, 1970,10(3): 220-226 LI S,LI P Y,LI W,et al. Effect of drought stress on proline and betaine metabolic pathway in Cynodon dactylon[J]. Chinese Journal of Grassland,2024,46(8):45-52 李硕,李培英,李雯,等. 干旱胁迫下不同抗性狗牙根脯氨酸和甜菜碱代谢变化[J]. 中国草地学报,2024,46(8):45-52 ZHU S B,WUERGULI T,TANG G M,et al. Research progress on saline-alkali land changes and its treatment measures in Xinjiang[J]. Shandong Agricultural Sciences,2023,55(3):158-165 朱生堡,乌尔古丽·托尔逊,唐光木,等. 新疆盐碱地变化及其治理措施研究进展[J]. 山东农业科学,2023,55(3):158-165 CHEN J B,LI D D,HAO D L,et al. Effects of pH value and nitrogen form on growth and sodium and potassium ion regulation of Cynodon dactylon under NaCl stress[J]. Journal of Plant Resources and Environment,2024,33(2):30-40 陈静波,李丹丹,郝东利,等. NaCl胁迫下pH值和氮素形态对狗牙根生长及钠钾离子调控的影响[J]. 植物资源与环境学报,2024,33(2):30-40 ISHIGURO S,NAKAMURA K. Characterization of a cDNA encoding a novel DNA-binding protein,SPF1 that recognizes SP8 sequences in the 5’ upstream regions of genes coding for sporamin and β-amylase from sweet potato[J]. Molecular and General Genetics MGG,1994,244(6):563-571 WANG Q S,WANG M H,ZHANG X Z,et al. WRKY gene family evolution in Arabidopsis thaliana[J]. Genetica,2011,139(8):973-983 EULGEM T,RUSHTON P J,ROBATZEK S,et al. The WRKY superfamily of plant transcription factors[J]. Trends in Plant Science,2000,5(5):199-206 FLOWERS T J,MUNNS R,COLMER T D. Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes[J]. Annals of Botany,2015,115(3):419-431 WANG R Y,WEN W W,ZHAO E H,et al. Cloning of alfalfa MsWRKY11 gene and analysis of its salt tolerance function[J]. Acta Prataculturae Sinica,2021,30(11):157-169 王如月,文武武,赵恩华,等. 紫花苜蓿MsWRKY11基因的克隆及其耐盐功能分析[J]. 草业学报,2021,30(11):157-169 YU Y A,WU Y X,HE L Y. A wheat WRKY transcription factor TaWRKY17 enhances tolerance to salt stress in transgenic Arabidopsis and wheat plant[J]. Plant Molecular Biology,2023,113(4):171-191 HUANG S Z,HU L J,ZHANG S H,et al. Rice OsWRKY50 mediates ABA-dependent seed germination and seedling growth,and ABA-independent salt stress tolerance[J]. International Journal of Molecular Sciences,2021,22(16):8625 SHAO A,XU X,AMOMBO E,et al. CdWRKY2 transcription factor modulates salt oversensitivity in bermudagrass [Cynodon dactylon (L.) Pers[J]. Frontiers in Plant Science,2023,14:1164534 HUANG X B,AMEE M,CHEN L. Bermudagrass CdWRKY50 gene negatively regulates plants’ response to salt stress[J]. Environmental and Experimental Botany,2021,188:104513 CHEN C J,CHEN H,ZHANG Y,et al. TBtools: an integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant,2020,13(8):1194-1202 GUIXIA XU C G. Divergence of duplicate genes in exon-intron structure[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(4):1187-1192 SZKLARCZYK D,GABLE A L,LYON D,et al. STRING v11: protein–protein association networks with increased coverage,supporting functional discovery in genome-wide experimental datasets[J]. Nucleic Acids Research,2019,47(D1):D607-D613 TANG L S. Morphological,physiological and molecular response mechanism of Cynodon dactylon in Xinjiang under alkaline salt stress[D]. Urumqi:Xinjiang Agricultural University,2024:7-18 汤丽斯. 碱性盐胁迫下新疆狗牙根形态、生理及分子响应机制研究[D]. 乌鲁木齐:新疆农业大学,2024:7-18 TANG L S,YU Q K,LI W,et al. Evaluation and identification of reference genes for qRT-PCR analysis in bermudagrass roots under alkaline salt stress[J]. Physiology and Molecular Biology of Plants,2025,31(5):729-738 ROSS C A,LIU Y,SHEN Q J. The WRKY gene family in rice (Oryza sativa)[J]. Journal of Integrative Plant Biology,2007,49(6):827-842 JIANG M,YIN L F,ZHANG Z X,et al. Identification and analysis of WRKY gene family members of Brachypodium bicolor[J]. Journal of Triticeae Crops,2012,32(6):1013-1020 蒋明,尹龙飞,张志仙,等. 二穗短柄草WRKY基因家族成员的鉴定和分析[J]. 麦类作物学报,2012,32(6):1013-1020 LI X Y,LIU H,HE F,et al. Identification and expression pattern of the WRKY transcription factor family in Medicago sativa[J]. Acta Prataculturae Sinica,2024,33(4):154-170 李显炀,刘昊,何飞,等. 全基因组水平紫花苜蓿WRKY转录因子家族鉴定与表达模式分析[J]. 草业学报,2024,33(4):154-170 MAO C Z,WANG L,KONG D D,et al. Bioinformatics analysis of WRKY gene family in barley genome[J]. Molecular Plant Breeding,2019,17(23):7623-7633 毛成志,王蕾,孔豆豆,等. 大麦全基因组WRKY基因家族生物信息学分析[J]. 分子植物育种,2019,17(23):7623-7633 CHEN Q,XU X Y,WANG J C,et al. Identification of a WRKY gene family based on full-length transcriptome sequences and analysis of response patterns under salt stress in Halogeton glomeratus[J]. Acta Prataculturae Sinica,2022,31(12):146-157 陈倩,徐晓芸,汪军成,等. 基于全长转录组的盐生草WRKY基因家族的鉴定及其盐胁迫响应模式分析[J]. 草业学报,2022,31(12):146-157 SHUI D J,SUN J,XIONG Z L,et al. Identification and pathogenic response of tomato WRKY transcription factors[J]. Fujian Journal of Agricultural Sciences,2023,38(3):281-293 水德聚,孙继,熊自立,等. 番茄WRKY转录因子比较鉴定及细菌胁迫响应[J]. 福建农业学报,2023,38(3):281-293 CHEN X J,LI C,WANG H,et al. WRKY transcription factors: evolution,binding,and action[J]. Phytopathology Research,2019,1(1):13 JIANG Y Q,DEYHOLOS M K. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses[J]. Plant Molecular Biology,2009,69(1):91-105 LIU W,JIANG L P,CHI Y J,et al. Identification and expression analysis of WRKY gene family in Coptis chinensis[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2022,24(4):1369-1380 刘微,蒋莉萍,池玉洁,等. 黄连WRKY基因家族鉴定及表达分析[J]. 世界科学技术-中医药现代化,2022,24(4):1369-1380 CANNON S B,MITRA A,BAUMGARTEN A,et al. The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana[J]. BMC Plant Biology,2004,4:10 ZHANG S S,RUAN S M,YANG W Z. Identification of WRKY gene family and analysis of drought stress expression in Poncirus Polyandra[J]. Journal of Fruit Science,2025,42(3):508-525 张珊珊,阮孙美,杨文忠. 富民枳WRKY基因家族的鉴定和干旱胁迫表达分析[J]. 果树学报,2025,42(3):508-525 YANG J T,LI P F,XIAO Q Q. Identification and functional analysis of WRKY gene family of Lonicera japonica thunb[J]. Journal of Southwest University (Natural Science Edition),2023,45(7):87-96 杨教童,李鹏飞,肖巧巧. 金银花WRKY基因家族的鉴定与功能分析[J]. 西南大学学报(自然科学版),2023,45(7):87-96 YU Y P,JIANG Y J,SUN X Y,et al. Identification of the WRKY gene family and analysis of its expression in response to drought stress in centipedegrass[J]. Acta Agrestia Sinica,2024,32(5):1378-1391 于元平,蒋宇佳,孙向一,等. 假俭草WRKY基因家族鉴定及其响应干旱胁迫表达分析[J]. 草地学报,2024,32(5):1378-1391 HE F,ZHANG L X,ZHAO G Q,et al. Genome-wide identification and expression analysis of the NAC gene family in alfalfa revealed its potential roles in response to multiple abiotic stresses[J]. International Journal of Molecular Sciences,2022,23(17):10015