Article(id=1309565369086476348, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309565023480021997, articleNumber=null, orderNo=null, doi=10.11733/j.issn.1007-0435.2026.01.010, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1739116800000, receivedDateStr=2025-02-10, revisedDate=1755792000000, revisedDateStr=2025-08-22, acceptedDate=null, acceptedDateStr=null, onlineDate=1790154314538, onlineDateStr=2026-09-23, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790154314538, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790154314538, creator=13701087609, updateTime=1790154314538, updator=13701087609, issue=Issue{id=1309565023480021997, tenantId=1146029695717560320, journalId=1308397196074385509, year='2026', volume='34', issue='1', pageStart='1', pageEnd='376', issueExtLink='null', onlineDate='null', pubDate='1768406400000', pubDateStr='2026-01-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790154232139, creator='13701087609', updateTime=1790156721646, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309575465308880991, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309565023480021997, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309575465308880992, tenantId=1146029695717560320, journalId=1308397196074385509, issueId=1309565023480021997, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=98, endPage=106, ext={EN=ArticleExt(id=1309565369552044094, articleId=1309565369086476348, tenantId=1146029695717560320, journalId=1308397196074385509, language=EN, title=Effects of Artificial Grassland Establishment on Soil-Microbial Stoichiometric Characteristics in the Three-River-Source Region, columnId=null, journalTitle=Acta Agrestia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=Soil-microbial biomass stoichiometric characteristics serve as a robust indicator of ecosystem nutrient cycling efficiency, yet the mechanisms by which different artificial grassland restoration modes influence these traits remain unclear. This study investigated 3-year-old artificial grasslands dominated by tall-grass species (SF), short-grass species (XF), and a mixed planting of tall- and short-grass species (SFXF) in the Three-River-Source Region, with severely degraded alpine grassland (CK) as the control. Soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), microbial biomass carbon (MBC), nitrogen (MBN), and phosphorus (MBP) in the 0-10 cm layer were measured to analyze their stoichiometric ratios and microbial quotients. The impacts of different restoration modes on soil-microbial stoichiometric balance were further elucidated.The results demonstrated that all three artificial grassland establishment modes significantly enhanced SOC, MBN, and MBP contents. Specifically, the SF treatment increased soil TN, whereas the XF treatment reduced TN and decreased TP by 50.42%. MBN incresed by 24.36% and 35.59% under SF and XF treatments, respectively. The SFXF treatment decreased the microbial C-quotient by 78.40%, while the XF treatment elevated the microbial N-quotient by 49.61%, and the SFXF treatment reduced it by 11.33%. After restoration, the stoichiometric imbalances in soil-microbial biomass (Cimb:Nimb, Cimb:Pimb, and Nimb:Pimb) increased across all treatments, with the most pronounced escalation observed in the SFXF treatment. Redundancy analysis (RDA) revealed that soil N:P and C:P ratios were key drivers of microbial quotient variations. In conclusion, the SFXF mixed planting exerted the most substantial influence on soil-microbial C-N-P cycling. For practical management, supplemental nitrogen fertilization is recommended to optimize plant growth and improve soil quality., authors=WANG Shou-xing1,2, A di-Ha-ze3,4, OU Li-peng1,2, LI Cheng-xian1,2, NING Xiao-chun1,2, WANG Yan-he1,2, WEI Dai-jun1,2, YANG Ming-xin1,2, GU Qiang1,2, ZHANG Zhong-hua3, ZHOU Hua-kun3, authorsList=WANG Shou-xing, A di-Ha-ze, OU Li-peng, LI Cheng-xian, NING Xiao-chun, WANG Yan-he, WEI Dai-jun, YANG Ming-xin, GU Qiang, ZHANG Zhong-hua, ZHOU Hua-kun, authorCompany=1 Xining Natural Resources Comprehensive Survey Center, China Geological Survey, Xining, Qinghai Province 810000, China; 2 Yellow River Source Water Resources and Ecological System Process Field Scientific Observation Research Station, Maduo, Qinghai Province 813500, China; 3 Qinghai Provincial Key Laboratory of Cold Regions Restoration Ecology, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China; 4 University of Chinese Academy of Sciences, Beijing 100049, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309565369455575101, articleId=1309565369086476348, tenantId=1146029695717560320, journalId=1308397196074385509, language=CN, title=三江源区人工草地建植对土壤-微生物化学计量特征的影响, columnId=1309565024058835950, journalTitle=草地学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=土壤-微生物生物量化学计量特征可有效表征生态系统养分循环效率,但不同人工草地恢复模式对其影响机制尚不明确。本研究以三江源区建植3年的上繁草(SF)、下繁草(XF)及上繁草+下繁草(SFXF)人工草地为研究对象,并以重度退化高寒草地(CK)为对照,采集0~10 cm土层样品,测定土壤有机碳、全氮、全磷及微生物量碳、氮、磷含量,分析其化学计量比与微生物熵的变化。研究结果表明,三种人工草地建植模式均提高了土壤有机碳、微生物量氮和磷含量。SF处理增加了土壤全氮含量,而XF处理则降低了全氮含量,并使全磷含量减少了50.42%。微生物量氮在SF和XF处理分别增加了24.36%和35.59%。SFXF处理使微生物熵碳降低了78.40%,而XF处理提高了微生物熵氮49.61%,SFXF处理则降低了微生物熵氮11.33%。三种人工草地建植后,土壤微生物化学计量不平衡性(Cimb:Nimb、Cimb:Pimb和Nimb:Pimb)均上升,其中SFXF处理的增幅最大。冗余分析表明,土壤氮磷比和碳磷比是调控微生物熵变化的关键因子。综上,SFXF混播模式对土壤-微生物系统碳氮磷循环的影响最为显著,在实际管理中建议适当增加氮素供给以优化植物生长并提升土壤质量。, authors=王守兴1,2, 阿的哈则3,4, 欧立鹏1,2, 李成先1,2, 宁晓春1,2, 王雁鹤1,2, 魏代军1,2, 杨明新1,2, 谷强1,2, 张中华3, 周华坤3, authorsList=王守兴, 阿的哈则, 欧立鹏, 李成先, 宁晓春, 王雁鹤, 魏代军, 杨明新, 谷强, 张中华, 周华坤, authorCompany=1 中国地质调查局西宁自然资源综合调查中心, 青海 西宁 810000; 2 黄河源水资源与生态系统过程野外科学观测研究站, 青海 玛多 813500; 3 中国科学院西北高原生物研究所, 青海省恢复生态学重点实验室, 青海 西宁 810000; 4 中国科学院大学, 北京 100049, correspAuthors=null, authorNote=王守兴(1991-),男,汉族,青海湟中人,工程师,本科,主要从事生态学研方向研究,E-mail:wangshx717@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=pqi0Xbqvp0pztbLT3f2Bzw==, 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aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1768406400000, fullTextJson=null, articleText=null, reference=KWABIAH A B,PALM C A,STOSKOPF N C,et al. Response of soil microbial biomass dynamics to quality of plant materials with emphasis on P availability[J]. Soil Biology and Biochemistry,2003,35(2):207-216 ZHOU X,SUN H,PUMPANEN J,et al. The impact of wildfire on microbial C∶N∶P stoichiometry and the fungal-to-bacterial ratio in permafrost soil[J]. Biogeochemistry,2019,142(1):1-17[LinkOut] MALÝ S,FIALA P,REININGER D,et al. The relationships among microbial parameters and the rate of organic matter mineralization in forest soils,as influenced by forest type[J]. Pedobiologia,2014,57(4/5/6):235-244 KASCHUK G,ALBERTON O,HUNGRIA M. Three decades of soil microbial biomass studies in Brazilian ecosystems: lessons learned about soil quality and indications for improving sustainability[J]. Soil Biology and Biochemistry,2010,42(1):1-13 WANG Q K,WANG S L,FENG Z W,et al. Active soil organic matter and its relationship with soil quality[J]. Acta Ecologica Sinica,2005,25(3):513-519 王清奎,汪思龙,冯宗炜,等. 土壤活性有机质及其与土壤质量的关系[J]. 生态学报,2005,25(3):513-519 YU H,LIU Y,DENG Y,et al. Effects of the transformation from natural alpine grassland to mixed artificial grassland on the characteristics of soil microbial community[J]. Environmental Science,2023,44(5):2928-2935 于皓,刘悦,邓晔,等. 天然高寒草地转变为混播人工草地对土壤微生物群落特征的影响[J]. 环境科学,2023,44(5):2928-2935 LI S X,YAO T,WANG L D,et al. Changes of soil microbial biomass during the restoration of the degraded alpine grasslands in eastern Qilian Mountains[J]. Acta Agrestia Sinica,2023,31(12):3668-3675 李双雄,姚拓,王理德,等. 祁连山东段退化高寒草地修复过程中土壤微生物生物量变化[J]. 草地学报,2023,31(12):3668-3675 A D,CHANG T,SU H Y,et al. Effects of monoculture and mixed-sown grasses on soil physicochemical properties and microbial biomass[J]. Acta Agrestia Sinica,2024,32(7):2072-2080 阿的哈则,常涛,苏洪烨,等. 单播和混播禾草对土壤理化性质和微生物生物量的影响[J]. 草地学报,2024,32(7):2072-2080 ZHOU Z H,WANG C K. Changes of the relationships between soil and microbes in carbon,nitrogen and phosphorus stoichiometry during ecosystem succession[J]. Chinese Journal of Plant Ecology,2016,40(12):1257-1266 周正虎,王传宽. 生态系统演替过程中土壤与微生物碳氮磷化学计量关系的变化[J]. 植物生态学报,2016,40(12):1257-1266 CHEN J,SEVEN J,ZILLA T,et al. Microbial C: N: P stoichiometry and turnover depend on nutrients availability in soil: a 14C,15N and 33P triple labelling study[J]. Soil Biology and Biochemistry,2019,131:206-216 JIANG S,WANG Q G. Response mechanism of soil microbial stoichiometry imbalance in a boreal forest: a review[J]. Hans Journal of Soil Science,2021(2):45-53 蒋帅,王庆贵. 北方森林土壤微生物化学计量不平衡响应机制研究进展[J]. 土壤科学,2021(2):45-53 HU Z D,LIU S R,LIU X L,et al. Soil and soil microbial biomass contents and C∶N∶P stoichiometry at different succession stages of natural secondary forest in sub-alpine area of western Sichuan,China[J]. Acta Ecologica Sinica,2021,41(12):4900-4912 胡宗达,刘世荣,刘兴良,等. 川西亚高山天然次生林不同演替阶段土壤-微生物生物量及其化学计量特征[J]. 生态学报,2021,41(12):4900-4912 JI J J,ZHENG W,YANG Z J,et al. Effects of subtropical forest conversion on soil microbial respiration and its entropy[J]. Acta Ecologica Sinica,2020,40(3):800-807 纪娇娇,郑蔚,杨智杰,等. 亚热带森林转换对土壤微生物呼吸及其熵值的影响[J]. 生态学报,2020,40(3):800-807 HU S L,DONG L G,BAI X X,et al. Soil microbial biomass and ecological stoichiometric characteristics of typical land use types in Loess Hilly Region[J]. Journal of Soil and Water Conservation,2024,38(3):298-305 胡斯乐,董立国,白晓雄,等. 黄土丘陵区典型土地利用类型土壤-微生物量及其生态化学计量特征[J]. 水土保持学报,2024,38(3):298-305 BANNING N C,GRANT C D,JONES D L,et al. Recovery of soil organic matter,organic matter turnover and nitrogen cycling in a post-mining forest rehabilitation chronosequence[J]. Soil Biology and Biochemistry,2008,40(8):2021-2031 ANDERSON T H. Microbial eco-physiological indicators to asses soil quality[J]. Agriculture,Ecosystems & Environment,2003,98(1/2/3):285-293 FAN Y X,YANG Y S,YANG Z J,et al. Seasonal dynamics and content of soil labile organic carbon of mid-subtropical evergreen broadleaved forest during natural succession[J]. Acta Ecologica Sinica,2013,33(18):5751-5759 范跃新,杨玉盛,杨智杰,等. 中亚热带常绿阔叶林不同演替阶段土壤活性有机碳含量及季节动态[J]. 生态学报,2013,33(18):5751-5759 FENG Q,BAI G Z,LI Z X,et al. Accelerate construction of new pattern of ecological protection in northwest China[J]. Bulletin of Chinese Academy of Sciences,2022,37(10):1457-1470 冯起,白光祖,李宗省,等. 加快构建西北地区生态保护新格局[J]. 中国科学院院刊,2022,37(10):1457-1470 MIEHE G,SCHLEUSS P M,SEEBER E,et al. The Kobresia pygmaea ecosystem of the Tibetan Highlands - Origin,functioning and degradation of the world’s largest pastoral alpine ecosystem: Kobresia pastures of Tibet[J]. Science of the Total Environment,2019,648:754-771 WU J S , LIN Q M, HUANG Q Y, et al. Methods for Measuring Soil Microbial Biomass and Their Applications [M]. Beijing: China Meteorological Press, 2006: 50-110 吴金水,林启美,黄巧云,等. 土壤微生物生物量测定方法及其应用[M]. 北京:气象出版社,2006:50-110 MOOSHAMMER M,WANEK W,ZECHMEISTER-BOLTENSTERN S,et al. Stoichiometric imbalances between terrestrial decomposer communities and their resources: mechanisms and implications of microbial adaptations to their resources[J]. Frontiers in Microbiology,2014(5):22 DONG L J,LI J H,CHEN S,et al. Changes in soil organic carbon content and their causes during the degradation of alpine meadows in Zoigê Wetland[J]. Chinese Journal of Plant Ecology,2021,45(5):507-515 董利军,李金花,陈珊,等. 若尔盖湿地高寒草甸退化过程中土壤有机碳含量变化及成因分析[J]. 植物生态学报,2021,45(5):507-515 WANG S Q,YU G R. Ecological stoichiometry characteristics of ecosystem carbon,nitrogen and phosphorus elements[J]. Acta Ecologica Sinica,2008,28(8):3937-3947 王绍强,于贵瑞. 生态系统碳氮磷元素的生态化学计量学特征[J]. 生态学报,2008,28(8):3937-3947 MA R T,HU F N,LIU J F,et al. Shifts in soil nutrient concentrations and C: N: P stoichiometry during long-term natural vegetation restoration[J]. PeerJ,2020,8:e8382 YANG Y H,LUO Y Q. Carbon∶nitrogen stoichiometry in forest ecosystems during stand development[J]. Global Ecology and Biogeography,2011,20(2):354-361 ZHU Q L,XING X Y,ZHANG H,et al. Soil ecological stoichiometry under different vegetation area on loess hillygully region[J]. Acta Ecologica Sinica,2013,33(15):4674-4682 朱秋莲,邢肖毅,张宏,等. 黄土丘陵沟壑区不同植被区土壤生态化学计量特征[J]. 生态学报,2013,33(15):4674-4682 WANG W,SARDANS J,ZENG C,et al. Responses of soil nutrient concentrations and stoichiometry to different human land uses in a subtropical tidal wetland[J]. Geoderma,2014,232:459-470 LUO D,LIU S,SHI Z M,et al. Soil microbial community structure in Picea asperata plantations with different ages in subalpine of western Sichuan,Southwest China[J]. Chinese Journal of Applied Ecology,2017,28(2):519-527 罗达,刘顺,史作民,等. 川西亚高山不同林龄云杉人工林土壤微生物群落结构[J]. 应用生态学报,2017,28(2):519-527 BAUHUS J,PARÉ D,TÉ L CO ̂. Effects of tree species,stand age and soil type on soil microbial biomass and its activity in a southern boreal forest[J]. Soil Biology and Biochemistry,1998,30(8/9):1077-1089 KAISER C,FUCHSLUEGER L,KORANDA M,et al. Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground C allocation[J]. Ecology,2011,92(5):1036-1051 YU F M,LIU H,LIU K H,et al. Changes of soil enzyme activities in the early period restoration of subalpine coniferous forests in western Sichuan,China[J]. Ecology and Environmental Sciences,2012,21(1):64-68 于方明,刘华,刘可慧,等. 川西亚高山暗针叶林恢复初期土壤酶活性研究[J]. 生态环境学报,2012,21(1):64-68 CHEN X,LIU Y,TANG S Y,et al. Characteristics of substrate quality variation at different litter decomposition stages in subalpine forest of western Sichuan[J]. Acta Botanica Boreali-Occidentalia Sinica,2017,37(3):586-594 谌贤,刘洋,唐实玉,等. 川西亚高山森林凋落物不同分解阶段基质质量特征[J]. 西北植物学报,2017,37(3):586-594 LIU X F,LIN T C,VADEBONCOEUR M A,et al. Root litter inputs exert greater influence over soil C than does aboveground litter in a subtropical natural forest[J]. Plant and Soil,2019,444(1):489-499 CLEVELAND C C,LIPTZIN D. C: N: P stoichiometry in soil: is there a “redfield ratio” for the microbial biomass?[J]. Biogeochemistry,2007,85(3):235-252 ZHOU Z H,WANG C K. Reviews and syntheses: soil resources and climate jointly drive variations in microbial biomass carbon and nitrogen in China’s forest ecosystems[J]. Biogeosciences,2015,12(22):6751-6760 RONDINA A B L,TONON B C,LESCANO L E A M,et al. Plants of distinct successional stages have different strategies for nutrient acquisition in an Atlantic rain forest ecosystem[J]. International Journal of Plant Sciences,2019,180(3):186-199 LI J W,LIU Y L,HAI X Y,et al. Dynamics of soil microbial C: N: P stoichiometry and its driving mechanisms following natural vegetation restoration after farmland abandonment[J]. Science of The Total Environment,2019,693:133613 WANG B R,YANG J J,AN S S,et al. Effects of vegetation and topography features on ecological stoichiometry of soil and soil microbial biomass in the hilly-gully region of the Loess Plateau,China[J]. Chinese Journal of Applied Ecology,2018,29(1):247-259 王宝荣,杨佳佳,安韶山,等. 黄土丘陵区植被与地形特征对土壤和土壤微生物生物量生态化学计量特征的影响[J]. 应用生态学报,2018,29(1):247-259 BUI E N,HENDERSON B L. C: N: P stoichiometry in Australian soils with respect to vegetation and environmental factors[J]. Plant and Soil,2013,373(1):553-568)
Effects of Artificial Grassland Establishment on Soil-Microbial Stoichiometric Characteristics in the Three-River-Source Region
WANG Shou-xing1,2, A di-Ha-ze3,4, OU Li-peng1,2, LI Cheng-xian1,2, NING Xiao-chun1,2, WANG Yan-he1,2, WEI Dai-jun1,2, YANG Ming-xin1,2, GU Qiang1,2, ZHANG Zhong-hua3, ZHOU Hua-kun3
Affiliations
1 Xining Natural Resources Comprehensive Survey Center, China Geological Survey, Xining, Qinghai Province 810000, China; 2 Yellow River Source Water Resources and Ecological System Process Field Scientific Observation Research Station, Maduo, Qinghai Province 813500, China; 3 Qinghai Provincial Key Laboratory of Cold Regions Restoration Ecology, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810008, China; 4 University of Chinese Academy of Sciences, Beijing 100049, China
Soil-microbial biomass stoichiometric characteristics serve as a robust indicator of ecosystem nutrient cycling efficiency, yet the mechanisms by which different artificial grassland restoration modes influence these traits remain unclear. This study investigated 3-year-old artificial grasslands dominated by tall-grass species (SF), short-grass species (XF), and a mixed planting of tall- and short-grass species (SFXF) in the Three-River-Source Region, with severely degraded alpine grassland (CK) as the control. Soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), microbial biomass carbon (MBC), nitrogen (MBN), and phosphorus (MBP) in the 0-10 cm layer were measured to analyze their stoichiometric ratios and microbial quotients. The impacts of different restoration modes on soil-microbial stoichiometric balance were further elucidated.The results demonstrated that all three artificial grassland establishment modes significantly enhanced SOC, MBN, and MBP contents. Specifically, the SF treatment increased soil TN, whereas the XF treatment reduced TN and decreased TP by 50.42%. MBN incresed by 24.36% and 35.59% under SF and XF treatments, respectively. The SFXF treatment decreased the microbial C-quotient by 78.40%, while the XF treatment elevated the microbial N-quotient by 49.61%, and the SFXF treatment reduced it by 11.33%. After restoration, the stoichiometric imbalances in soil-microbial biomass (Cimb:Nimb, Cimb:Pimb, and Nimb:Pimb) increased across all treatments, with the most pronounced escalation observed in the SFXF treatment. Redundancy analysis (RDA) revealed that soil N:P and C:P ratios were key drivers of microbial quotient variations. In conclusion, the SFXF mixed planting exerted the most substantial influence on soil-microbial C-N-P cycling. For practical management, supplemental nitrogen fertilization is recommended to optimize plant growth and improve soil quality.
WANG Shou-xing, A di-Ha-ze, OU Li-peng, LI Cheng-xian, NING Xiao-chun, WANG Yan-he, WEI Dai-jun, YANG Ming-xin, GU Qiang, ZHANG Zhong-hua, ZHOU Hua-kun.
Effects of Artificial Grassland Establishment on Soil-Microbial Stoichiometric Characteristics in the Three-River-Source Region[J].
Acta Agrestia Sinica,
2026
, 34
(1)
: 98
-106
.
DOI: 10.11733/j.issn.1007-0435.2026.01.010
KWABIAH A B,PALM C A,STOSKOPF N C,et al. Response of soil microbial biomass dynamics to quality of plant materials with emphasis on P availability[J]. Soil Biology and Biochemistry,2003,35(2):207-216 ZHOU X,SUN H,PUMPANEN J,et al. The impact of wildfire on microbial C∶N∶P stoichiometry and the fungal-to-bacterial ratio in permafrost soil[J]. Biogeochemistry,2019,142(1):1-17[LinkOut] MALÝ S,FIALA P,REININGER D,et al. The relationships among microbial parameters and the rate of organic matter mineralization in forest soils,as influenced by forest type[J]. Pedobiologia,2014,57(4/5/6):235-244 KASCHUK G,ALBERTON O,HUNGRIA M. Three decades of soil microbial biomass studies in Brazilian ecosystems: lessons learned about soil quality and indications for improving sustainability[J]. Soil Biology and Biochemistry,2010,42(1):1-13 WANG Q K,WANG S L,FENG Z W,et al. Active soil organic matter and its relationship with soil quality[J]. Acta Ecologica Sinica,2005,25(3):513-519 王清奎,汪思龙,冯宗炜,等. 土壤活性有机质及其与土壤质量的关系[J]. 生态学报,2005,25(3):513-519 YU H,LIU Y,DENG Y,et al. Effects of the transformation from natural alpine grassland to mixed artificial grassland on the characteristics of soil microbial community[J]. Environmental Science,2023,44(5):2928-2935 于皓,刘悦,邓晔,等. 天然高寒草地转变为混播人工草地对土壤微生物群落特征的影响[J]. 环境科学,2023,44(5):2928-2935 LI S X,YAO T,WANG L D,et al. Changes of soil microbial biomass during the restoration of the degraded alpine grasslands in eastern Qilian Mountains[J]. Acta Agrestia Sinica,2023,31(12):3668-3675 李双雄,姚拓,王理德,等. 祁连山东段退化高寒草地修复过程中土壤微生物生物量变化[J]. 草地学报,2023,31(12):3668-3675 A D,CHANG T,SU H Y,et al. Effects of monoculture and mixed-sown grasses on soil physicochemical properties and microbial biomass[J]. Acta Agrestia Sinica,2024,32(7):2072-2080 阿的哈则,常涛,苏洪烨,等. 单播和混播禾草对土壤理化性质和微生物生物量的影响[J]. 草地学报,2024,32(7):2072-2080 ZHOU Z H,WANG C K. Changes of the relationships between soil and microbes in carbon,nitrogen and phosphorus stoichiometry during ecosystem succession[J]. Chinese Journal of Plant Ecology,2016,40(12):1257-1266 周正虎,王传宽. 生态系统演替过程中土壤与微生物碳氮磷化学计量关系的变化[J]. 植物生态学报,2016,40(12):1257-1266 CHEN J,SEVEN J,ZILLA T,et al. Microbial C: N: P stoichiometry and turnover depend on nutrients availability in soil: a 14C,15N and 33P triple labelling study[J]. Soil Biology and Biochemistry,2019,131:206-216 JIANG S,WANG Q G. Response mechanism of soil microbial stoichiometry imbalance in a boreal forest: a review[J]. Hans Journal of Soil Science,2021(2):45-53 蒋帅,王庆贵. 北方森林土壤微生物化学计量不平衡响应机制研究进展[J]. 土壤科学,2021(2):45-53 HU Z D,LIU S R,LIU X L,et al. Soil and soil microbial biomass contents and C∶N∶P stoichiometry at different succession stages of natural secondary forest in sub-alpine area of western Sichuan,China[J]. Acta Ecologica Sinica,2021,41(12):4900-4912 胡宗达,刘世荣,刘兴良,等. 川西亚高山天然次生林不同演替阶段土壤-微生物生物量及其化学计量特征[J]. 生态学报,2021,41(12):4900-4912 JI J J,ZHENG W,YANG Z J,et al. Effects of subtropical forest conversion on soil microbial respiration and its entropy[J]. Acta Ecologica Sinica,2020,40(3):800-807 纪娇娇,郑蔚,杨智杰,等. 亚热带森林转换对土壤微生物呼吸及其熵值的影响[J]. 生态学报,2020,40(3):800-807 HU S L,DONG L G,BAI X X,et al. Soil microbial biomass and ecological stoichiometric characteristics of typical land use types in Loess Hilly Region[J]. Journal of Soil and Water Conservation,2024,38(3):298-305 胡斯乐,董立国,白晓雄,等. 黄土丘陵区典型土地利用类型土壤-微生物量及其生态化学计量特征[J]. 水土保持学报,2024,38(3):298-305 BANNING N C,GRANT C D,JONES D L,et al. Recovery of soil organic matter,organic matter turnover and nitrogen cycling in a post-mining forest rehabilitation chronosequence[J]. Soil Biology and Biochemistry,2008,40(8):2021-2031 ANDERSON T H. Microbial eco-physiological indicators to asses soil quality[J]. Agriculture,Ecosystems & Environment,2003,98(1/2/3):285-293 FAN Y X,YANG Y S,YANG Z J,et al. Seasonal dynamics and content of soil labile organic carbon of mid-subtropical evergreen broadleaved forest during natural succession[J]. Acta Ecologica Sinica,2013,33(18):5751-5759 范跃新,杨玉盛,杨智杰,等. 中亚热带常绿阔叶林不同演替阶段土壤活性有机碳含量及季节动态[J]. 生态学报,2013,33(18):5751-5759 FENG Q,BAI G Z,LI Z X,et al. Accelerate construction of new pattern of ecological protection in northwest China[J]. Bulletin of Chinese Academy of Sciences,2022,37(10):1457-1470 冯起,白光祖,李宗省,等. 加快构建西北地区生态保护新格局[J]. 中国科学院院刊,2022,37(10):1457-1470 MIEHE G,SCHLEUSS P M,SEEBER E,et al. The Kobresia pygmaea ecosystem of the Tibetan Highlands - Origin,functioning and degradation of the world’s largest pastoral alpine ecosystem: Kobresia pastures of Tibet[J]. Science of the Total Environment,2019,648:754-771 WU J S , LIN Q M, HUANG Q Y, et al. Methods for Measuring Soil Microbial Biomass and Their Applications [M]. Beijing: China Meteorological Press, 2006: 50-110 吴金水,林启美,黄巧云,等. 土壤微生物生物量测定方法及其应用[M]. 北京:气象出版社,2006:50-110 MOOSHAMMER M,WANEK W,ZECHMEISTER-BOLTENSTERN S,et al. Stoichiometric imbalances between terrestrial decomposer communities and their resources: mechanisms and implications of microbial adaptations to their resources[J]. Frontiers in Microbiology,2014(5):22 DONG L J,LI J H,CHEN S,et al. Changes in soil organic carbon content and their causes during the degradation of alpine meadows in Zoigê Wetland[J]. Chinese Journal of Plant Ecology,2021,45(5):507-515 董利军,李金花,陈珊,等. 若尔盖湿地高寒草甸退化过程中土壤有机碳含量变化及成因分析[J]. 植物生态学报,2021,45(5):507-515 WANG S Q,YU G R. Ecological stoichiometry characteristics of ecosystem carbon,nitrogen and phosphorus elements[J]. Acta Ecologica Sinica,2008,28(8):3937-3947 王绍强,于贵瑞. 生态系统碳氮磷元素的生态化学计量学特征[J]. 生态学报,2008,28(8):3937-3947 MA R T,HU F N,LIU J F,et al. Shifts in soil nutrient concentrations and C: N: P stoichiometry during long-term natural vegetation restoration[J]. PeerJ,2020,8:e8382 YANG Y H,LUO Y Q. Carbon∶nitrogen stoichiometry in forest ecosystems during stand development[J]. Global Ecology and Biogeography,2011,20(2):354-361 ZHU Q L,XING X Y,ZHANG H,et al. Soil ecological stoichiometry under different vegetation area on loess hillygully region[J]. Acta Ecologica Sinica,2013,33(15):4674-4682 朱秋莲,邢肖毅,张宏,等. 黄土丘陵沟壑区不同植被区土壤生态化学计量特征[J]. 生态学报,2013,33(15):4674-4682 WANG W,SARDANS J,ZENG C,et al. Responses of soil nutrient concentrations and stoichiometry to different human land uses in a subtropical tidal wetland[J]. Geoderma,2014,232:459-470 LUO D,LIU S,SHI Z M,et al. Soil microbial community structure in Picea asperata plantations with different ages in subalpine of western Sichuan,Southwest China[J]. Chinese Journal of Applied Ecology,2017,28(2):519-527 罗达,刘顺,史作民,等. 川西亚高山不同林龄云杉人工林土壤微生物群落结构[J]. 应用生态学报,2017,28(2):519-527 BAUHUS J,PARÉ D,TÉ L CO ̂. Effects of tree species,stand age and soil type on soil microbial biomass and its activity in a southern boreal forest[J]. Soil Biology and Biochemistry,1998,30(8/9):1077-1089 KAISER C,FUCHSLUEGER L,KORANDA M,et al. Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground C allocation[J]. Ecology,2011,92(5):1036-1051 YU F M,LIU H,LIU K H,et al. Changes of soil enzyme activities in the early period restoration of subalpine coniferous forests in western Sichuan,China[J]. Ecology and Environmental Sciences,2012,21(1):64-68 于方明,刘华,刘可慧,等. 川西亚高山暗针叶林恢复初期土壤酶活性研究[J]. 生态环境学报,2012,21(1):64-68 CHEN X,LIU Y,TANG S Y,et al. Characteristics of substrate quality variation at different litter decomposition stages in subalpine forest of western Sichuan[J]. Acta Botanica Boreali-Occidentalia Sinica,2017,37(3):586-594 谌贤,刘洋,唐实玉,等. 川西亚高山森林凋落物不同分解阶段基质质量特征[J]. 西北植物学报,2017,37(3):586-594 LIU X F,LIN T C,VADEBONCOEUR M A,et al. Root litter inputs exert greater influence over soil C than does aboveground litter in a subtropical natural forest[J]. Plant and Soil,2019,444(1):489-499 CLEVELAND C C,LIPTZIN D. C: N: P stoichiometry in soil: is there a “redfield ratio” for the microbial biomass?[J]. Biogeochemistry,2007,85(3):235-252 ZHOU Z H,WANG C K. Reviews and syntheses: soil resources and climate jointly drive variations in microbial biomass carbon and nitrogen in China’s forest ecosystems[J]. Biogeosciences,2015,12(22):6751-6760 RONDINA A B L,TONON B C,LESCANO L E A M,et al. Plants of distinct successional stages have different strategies for nutrient acquisition in an Atlantic rain forest ecosystem[J]. International Journal of Plant Sciences,2019,180(3):186-199 LI J W,LIU Y L,HAI X Y,et al. Dynamics of soil microbial C: N: P stoichiometry and its driving mechanisms following natural vegetation restoration after farmland abandonment[J]. Science of The Total Environment,2019,693:133613 WANG B R,YANG J J,AN S S,et al. Effects of vegetation and topography features on ecological stoichiometry of soil and soil microbial biomass in the hilly-gully region of the Loess Plateau,China[J]. Chinese Journal of Applied Ecology,2018,29(1):247-259 王宝荣,杨佳佳,安韶山,等. 黄土丘陵区植被与地形特征对土壤和土壤微生物生物量生态化学计量特征的影响[J]. 应用生态学报,2018,29(1):247-259 BUI E N,HENDERSON B L. C: N: P stoichiometry in Australian soils with respect to vegetation and environmental factors[J]. Plant and Soil,2013,373(1):553-568