1 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan Province 410221, China; 2 Key laboratory of Genetic Breeding and Microbial Processing for Bast Fiber Crops, MARA, Changsha, Hunan Province 410221, China; 3 Yuelushan Laboratory, Changsha, Hunan Province 410128, China
Glutathione S-transferases （GSTs） play an important role in the regulation of plants against stressful environments， but the systematic studies on Ramie were lack. In this paper， a total of 43 BnGSTs gene families were identified by bioinformatic analyses， most of which were predicted to be located in the cytoplasm and belonged to the plant-specific Tau and Phi class. Inter-species covariance analysis revealed that BnGSTs genes represented more orthologous genes in dicots than monocots； intra-species covariance analysis revealed that there were five pairs of paralogous genes among BnGSTs， all of which were derived from the whole genome or fragmentary gene duplication， and all of them were found that Ka/Ks ratios were less than 1， indicating that they were subjected to purifying selection. Cis-acting element prediction revealed that the upstream of these BnGSTs genes was rich in light-responsive， phytohormone-regulated and stress-responsive elements combined with transcriptome expression data， nine tissue-specific candidate genes were screened with significant （P<0.05） changes on expression patterns in roots， stems and leaves under cadmium stress. Two genes， BnGST22 and BnGST12， were identified as having significant up-regulation and high relative expression， which could be used as target genes for further functional verification. This study further enriched the functional gene pool of ramie and provided a theoretical basis for the use of anti-stress resources and variety selection.
DIXON D， LAPTHORN A， EDWARDS R. Plant glutathione transferases[J]. Genome Biology， 2002， 3（3）： reviews 3004. YAN H F， MAO P S， XIA F S. Research progress in plant antioxidant glutathione（review）[J]. Acta Agrestia Sinica， 2013， 21（3）： 428-434 闫慧芳， 毛培胜， 夏方山. 植物抗氧化剂谷胱甘肽研究进展[J]. 草地学报， 2013， 21（3）： 428-434 NUTRICATI E， MICELI A， BLANDO F， et al. Characterization of two Arabidopsis thaliana glutathione S-transferases[J]. Plant Cell Reports， 2006， 25： 997-1005 MCGONIGLE B， KEELER S J， LAU S C， et al. A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize[J]. Plant Physiology， 2000， 124： 1105-1120 SORANZO N， SARI GORLA M， MIZZI L， et al. Organisation and structural evolution of the rice glutathione S-transferase gene family[J]. Molecular Genetics and Genomics， 2004， 271： 511-521 CAO Q， LV W， JIANG H， et al. Genome-wide identification of glutathione S-transferase gene family members in tea plant （Camellia sinensis） and their response to environmental stress[J]. International Journal of Biological Macromolecules， 2022， 205： 749-760 JIANG W， WANG T， ZHANG M， et al. Genome-wide identification of glutathione S-transferase family from Dendrobium officinale and the functional characterization of DoGST5 in cadmium tolerance[J]. International Journal of Molecular Sciences， 2024， 25： 8439 WANG L， FU H， ZHAO J， et al. Genome-wide identification and expression profiling of glutathione S-transferase gene family in foxtail millet （Setaria italica L.）[J]. Plants， 2023， 12： 1138 NIANIOU-OBEIDAT I， MADESIS P， KISSOUDIS C， et al. Plant glutathione transferase-mediated stress tolerance： functions and biotechnological applications[J]. Plant Cell Reports， 2017， 36： 791 - 805 GILL S S， TUTEJA N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry， 2010， 48： 909-930 KUMAR S， ASIF M H， CHAKRABARTY D， et al. Expression of a rice Lambda class of glutathione S-transferase， OsGSTL2， in Arabidopsis provides tolerance to heavy metal and other abiotic stresses[J]. Journal of Hazardous Materials， 2013， 248-249： 228-237 SUN Y， TIAN Z， ZUO D， et al. GhUBC10‐2 mediates GhGSTU17 degradation to regulate salt tolerance in cotton （Gossypium hirsutum）[J]. Plant， Cell & Environment， 2024， 47： 1606-1624 CHEN J， JIANG H， HSIEH E， et al. Drought and salt stress tolerance of an Arabidopsis glutathione s-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid[J]. Plant Physiology， 2012， 158： 340-351 TAKESAWA T， ITO M， KANZAKI H， et al. Over-expression of ζ glutathione S-transferase in transgenic rice enhances germination and growth at low temperature[J]. Molecular Breeding， 2002， 9： 93-101 HAN Q， CHEN R， YANG Y， et al. A glutathione S-transferase gene from Lilium regale Wilson confers transgenic tobacco resistance to Fusarium oxysporum[J]. Scientia Horticulturae， 2016， 198： 370-378 GEORGE S， VENKATARAMAN G， PARIDA A. A chloroplast-localized and auxin-induced glutathione S-transferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco[J]. Journal of Plant Physiology， 2010， 167： 311-318 SECKIN DINLER B， CETINKAYA H， SECGIN Z. The regulation of glutathione s-transferases by gibberellic acid application in salt treated maize leaves[J]. Physiology and Molecular Biology of Plants， 2023， 29： 69-85 LOYALL L， UCHIDA K， BRAUN S， et al. Glutathione and a UV light-induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures[J]. The Plant Cell， 2000， 12： 1939-1950 JIANG H， LIU M， CHEN I， et al. A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development[J]. Plant Physiology， 2010， 154： 1646-1658 REHMAN M， GANG D， LIU Q， et al. Ramie， a multipurpose crop： potential applications， constraints and improvement strategies[J]. Industrial Crops and Products， 2019， 137： 300-307 WU Z， TANG Q， WANG Y， et al. Ramie （Boehmeria Nivea） as phytoremediation crop for heavy metal-contaminated paddy soil in southern China： variety comparison， Cd accumulation， and assessment of fiber recycling[J]. Journal of Natural Fibers， 2021， 19： 11078-11091 ZHANG X Y， HE S， QU H Y， et al. Bioinformatic and Expression Profiling Analysis of the CCCH Gene Family in Ramie[J]. Acta Agrestia Sinica， 2024， 32（3）： 703-713 张晓洋， 何思， 瞿宏悦， 等. 苎麻CCCH基因家族生物信息学及表达谱分析[J]. 草地学报， 2024， 32（3）： 703-713 LIU T， FU Y， LI G， et al. Transcriptomic and physiological responses of Qingye Ramie to drought stress[J]. Agronomy， 2024， 14： 301 AN X， CHEN J， ZHANG J， et al. Transcriptome profiling and identification of transcription factors in ramie （Boehmeria nivea L. Gaud.） in response to PEG treatment， using Illumina paired-end sequencing technology[J]. International Journal of Molecular Sciences， 2015， 16： 3493-3511 BAO Y， HUANG X， REHMAN M， et al. Identification and expression analysis of the PIN and AUX/LAX gene families in ramie （Boehmeria nivea L. Gaud.）[J]. Agronomy， 2019， 9： 435 BAO Y， ZOU Y， AN X， et al. Overexpression of a ramie （Boehmeria nivea L. Gaud.） Group I WRKY gene， BnWRKY49， increases drought resistance in Arabidopsis thaliana[J]. Plants， 2024， 13： 379 MA Y， JIE H， ZHAO L， et al. BnXTH1 regulates cadmium tolerance by modulating vacuolar compartmentalization and the cadmium binding capacity of cell walls in ramie （Boehmeria nivea）[J]. Journal of Hazardous Materials， 2024， 470： 134172 ZHU S J， SHI W J. Cloning and expression analysis of a glutathione-s-transferase gene BnGSTU1 from ramie （Boehmeria nivea （L.） Gaudich.）[J]. Journal of Plant Genetic Resources， 2018， 19（6）： 1197-1204 朱守晶， 史文娟. 苎麻谷胱甘肽-S-转移酶基因BnGSTU1的克隆和表达分析[J]. 植物遗传资源学报， 2018， 19（6）： 1197-1204 ZHANG Y， SHI C Y WANG Y M， et al. Expression characteristics of Bn-glutathione transferases gene in different ramie （Boehmeria nivea） varieties under cadmium stress[J]. Molecular Plant Breeding， 2022， 20（21）： 6975-6985 张英， 石朝艳， 汪娅梅， 等. 不同苎麻品种镉胁迫下谷胱甘肽硫转移酶基因的表达特征[J]. 分子植物育种， 2022， 20（21）： 6975-6985 LAMESCH P， DREHER K， SWARBRECK D， et al. Using the Arabidopsis Information Resource （TAIR） to find information about Arabidopsis genes[J]. Current Protocols in Bioinformatics， 2010， 30： 1-51 CHEN K， MING Y， LUAN M， et al. The chromosome-level assembly of ramie （Boehmeria nivea L.） genome provides insights into molecular regulation of fiber fineness[J]. Journal of Natural Fibers， 2023， 20： 2168819 MISTRY J， CHUGURANSKY S， WILLIAMS L， et al. Pfam： The protein families database in 2021[J]. Nucleic Acids Research， 2020， 49： D412-D419 JOHNSON M， ZARETSKAYA I， RAYTSELIS Y， et al. NCBI BLAST： a better web interface[J]. Nucleic Acids Research， 2008， 36： W5-W9 MISTRY J， FINN RD， EDDY SR， et al. Challenges in homology search： HMMER3 and convergent evolution of coiled-coil regions[J]. Nucleic Acids Research， 2013， 41： e121 ARTIMO P， JONNALAGEDDA M， ARNOLD K， et al. ExPASy： SIB bioinformatics resource portal[J]. Nucleic Acids Research， 2012， 40： W597-W603 YU C， CHEN Y， LU C， et al. Prediction of protein subcellular localization[J]. Proteins： Structure， Function， and Bioinformatics， 2006， 64： 643-651 GEOURJON C， DELÉAGE G. SOPMA： significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J]. Bioinformatics， 1995， 11： 681-684 CHEN C， WU Y， LI J， et al. TBtools-II： A “one for all， all for one” bioinformatics platform for biological big-data mining[J]. Molecular Plant， 2023， 16： 1733-1742 WANG Y， TANG H， DEBARRY J D， et al. MCScanX： a toolkit for detection and evolutionary analysis of gene synteny and collinearity[J]. Nucleic Acids Research， 2012， 40（7）： e49 WANG D， ZHANG Y， ZHANG Z， et al. KaKs_Calculator 2.0： A toolkit incorporating gamma-series methods and sliding window strategies[J]. Genomics， Proteomics & Bioinformatics， 2010， 8： 77-80 TAMURA K， STECHER G， KUMAR S. MEGA11： Molecular Evolutionary Genetics Analysis Version 11[J]. Molecular Biology and Evolution， 2021， 38： 3022-3027 SUBRAMANIAN B， GAO S， LERCHER M J， et al. Evolview v3： a webserver for visualization， annotation， and management of phylogenetic trees[J]. Nucleic Acids Research， 2019， 47： W270-W275 BAILEY T L， BODEN M， BUSKE F A， et al. MEME SUITE： tools for motif discovery and searching[J]. Nucleic Acids Research， 2009， 37： W202-W208 LESCOT M. PlantCARE， a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Research， 2002， 30： 325-327 KRYUCHKOVA-MOSTACCI N， ROBINSON-RECHAVI M. A benchmark of gene expression tissue-specificity metrics[J]. Briefings in Bioinformatics， 2017， 18（2）： 205-214 YANAI I， BENJAMIN H， SHMOISH M， et al. Genome-wide midrange transcription profiles reveal expression level relationships in human tissue specification[J]. Bioinformatics， 2005， 21： 650-659 SHAO D， ABUBAKAR AS， CHEN J， et al. Physiological， molecular， and morphological adjustment to waterlogging stress in ramie and selection of waterlogging-tolerant varieties[J]. Plant Physiology and Biochemistry， 2024， 216： 109101 MARRS K A. The functions and regulation of glutathione s-transferases in plants[J]. Annual Review of Plant Physiology and Plant Molecular Biology， 1996， 47： 127-158 HAYES J D， FLANAGAN J U， JOWSEY I R. Glutathione transferases[J]. Annual Review of Pharmacology and Toxicology， 2005， 45： 51-88 FROVA C. The plant glutathione transferase gene family： genomic structure， functions， expression and evolution[J]. Physiologia Plantarum， 2003， 119： 469-479 OAKLEY A J. Glutathione transferases： new functions[J]. Current Opinion in Structural Biology， 2005， 15： 716-723 KUMAR S， TRIVEDI P K. Glutathione S-transferases： Role in combating abiotic stresses including arsenic detoxification in plants[J]. Frontiers in Plant Science， 2018， 9： 751 CICERO L LO， MADESIS P， TSAFTARIS A， et al. Tobacco plants over-expressing the sweet orange tau glutathione transferases （CsGSTUs） acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses[J]. Phytochemistry， 2015， 116： 69-77 XU J， TIAN Y， XING X， et al. Over‐expression of AtGSTU19 provides tolerance to salt， drought and methyl viologen stresses in Arabidopsis[J]. Physiologia Plantarum， 2015， 156： 164-175 GUO R， LI S， GAO Y Q， et al. A novel OsGST gene encoding 9 glutathione reductase negatively regulates cadmium accumulation in rice[J]. Journal of Hazardous Materials， 2024， 476： 135126 ZHU S， LI W， YAN S， et al. Transcriptomic analysis of differentially expressed genes in Arabidopsis thaliana overexpressing BnMYB2 from Boehmeria nivea under cadmium stress[J]. Catalysts， 2023， 13： 662 ZHU S， SHI W， JIE Y， et al. A MYB transcription factor， BnMYB2， cloned from ramie （Boehmeria nivea） is involved in cadmium tolerance and accumulation[J]. PLoS One， 2020， 15： e0233375
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